CF832535

Overview
NameCF832535
Unique NameCF832535
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length651
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_BORBZ (Ribosome-recycling factor OS=Borrelia burgdorferi (strain ZS7) GN=frr PE=3 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 4.145e-30
Identity = 62/139 (44.60%), Postives = 99/139 (71.22%), Query Frame = -2
Query:  222 MLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 638
            + DKI ++Y+G    +  ++ I  P++  ++IQP+DK+ L  IE+AI++SDL M P++DG VIR+ +P LTSERR+++ K   K AEE K++ RNIR+D     +K +KE +++ED++K +  D+QK TD Y+KKID+I
Sbjct:   35 IFDKIFIQYHGQRTPITQVSSIRIPEARLVVIQPWDKTILNKIEQAILNSDLSMNPSSDGSVIRIKVPALTSERRQDIVKHAKKIAEEHKISTRNIRQDLNNKVKKQEKESEITEDSLKRILDDIQKSTDIYIKKIDAI 173          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_BORBU (Ribosome-recycling factor OS=Borrelia burgdorferi GN=frr PE=3 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 4.145e-30
Identity = 64/139 (46.04%), Postives = 97/139 (69.78%), Query Frame = -2
Query:  222 MLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 638
            + D+I ++Y+     +  ++ I  P++  ++IQP+DKS L  IE+AI++SDL M P+NDG VIR+ +P LTSERR+E+ K   K AEE K++ RNIR+D     +K +KE +++ED +K +  D+QK TD Y+KKIDSI
Sbjct:   35 IFDRICIQYHDRRTPITQVSSIRIPEARLVVIQPWDKSILNKIEQAILNSDLSMNPSNDGSVIRIKVPNLTSERRQEIVKHAKKIAEEHKISTRNIRQDLNNKVKKQEKESEITEDCLKRILDDIQKSTDMYIKKIDSI 173          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_BORAP (Ribosome-recycling factor OS=Borrelia afzelii (strain PKo) GN=frr PE=3 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 4.145e-30
Identity = 62/139 (44.60%), Postives = 99/139 (71.22%), Query Frame = -2
Query:  222 MLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 638
            + DKI ++Y+G    +  ++ I  P++  ++IQP+DK+ L  IE+AI++SDL M P++DG VIR+ +P LTSERR+++ K   K AEE K++ RNIR+D     +K +KE +++ED++K +  D+QK TD Y+KKID+I
Sbjct:   35 IFDKIFIQYHGQRTPITQVSSIRIPEARLVVIQPWDKTILNKIEQAILNSDLSMNPSSDGSVIRIKVPALTSERRQDIVKHAKKIAEEHKISTRNIRQDLNNKVKKQEKESEITEDSLKRILDDIQKSTDIYIKKIDAI 173          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_VIBF1 (Ribosome-recycling factor OS=Vibrio fischeri (strain ATCC 700601 / ES114) GN=frr PE=3 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 5.414e-30
Identity = 67/143 (46.85%), Postives = 95/143 (66.43%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            ++P++L  I VEYYG+   L  +A +   DS +L I  +DK     +EKAI+ SDLG+ P + G VIR+ LP LT ERRK+L K+V  +AE G+VA+RNIRRDA    + L KEK++SED+ +    ++QKLTD  +K ID +
Sbjct:   32 AHPSLLSGISVEYYGAQTPLNQVANVVAEDSRTLAITVFDKELAGLVEKAIMMSDLGLNPMSAGTVIRVPLPPLTEERRKDLVKIVRGEAENGRVAIRNIRRDANGDVKALLKEKEISEDDDRRAQDEIQKLTDAAVKSIDEV 174          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_TREDE (Ribosome-recycling factor OS=Treponema denticola GN=frr PE=3 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 5.414e-30
Identity = 63/143 (44.06%), Postives = 94/143 (65.73%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            ++ A+ DKI V  YG    L  +A I+ P++  ++IQP+DK  L  IEKA++ +DL + P NDG+VIR+ +P LT +RRK+L+K     AE  +V+VRNIRRD +   +KLQK+ K+SED +K      QK TD Y+ +I+ +
Sbjct:   32 ASSALFDKIRVNCYGESTPLNQLANISIPEARLVVIQPWDKGLLVEIEKAVLQADLSVNPTNDGKVIRIAIPPLTEDRRKDLAKKAKTIAENSRVSVRNIRRDGIDEAKKLQKDGKISEDQLKTAEDAFQKSTDAYIAEINKV 174          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_SALTO (Ribosome-recycling factor OS=Salinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440) GN=frr PE=3 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 5.414e-30
Identity = 62/145 (42.76%), Postives = 93/145 (64.14%), Query Frame = -2
Query:  216 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSIYK 650
            +N AM  KI ++YYGSP  L  +A I  P+   ++I+PYD S   ++EKAI  SDLG+ PNN+G  +R+ LPQ+T ERR+E+ KV   + EE KVAVRN+RR A +  ++L K  ++ ED+ +    +L  LT  ++  +D + K
Sbjct:   32 ANAAMFSKIVIDYYGSPTPLPQMASIGVPEPRMVIIKPYDTSQTNAMEKAIRDSDLGVNPNNEGNQLRILLPQMTEERRREMIKVARHKGEEAKVAVRNVRRKAKEELDRLVKAGEVGEDDGRRAEKELDDLTQRFVGTVDELIK 176          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_RHOPT (Ribosome-recycling factor OS=Rhodopseudomonas palustris (strain TIE-1) GN=frr PE=3 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 5.414e-30
Identity = 62/143 (43.36%), Postives = 100/143 (69.93%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            ++ +ML+ ++V+ YGS + L  +A ++ P+   + +Q +DKS +K++EK IV S+LG++P  +G+VIRL +P+L  ERRKEL KV  K AE  +VAVR++RRD L   +KL+K  ++SED+ K L  ++QK TD  + +ID +
Sbjct:   33 ASASMLEPVQVDAYGSHMPLNQVATVSVPEPRLISVQVWDKSMVKAVEKGIVDSNLGLSPATEGQVIRLRIPELNEERRKELVKVAHKYAEAARVAVRHVRRDGLDTIKKLEKAHEISEDDQKRLDQEVQKATDAAIAEIDQL 175          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_RHOPA (Ribosome-recycling factor OS=Rhodopseudomonas palustris GN=frr PE=3 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 5.414e-30
Identity = 62/143 (43.36%), Postives = 100/143 (69.93%), Query Frame = -2
Query:  222 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSI 650
            ++ +ML+ ++V+ YGS + L  +A ++ P+   + +Q +DKS +K++EK IV S+LG++P  +G+VIRL +P+L  ERRKEL KV  K AE  +VAVR++RRD L   +KL+K  ++SED+ K L  ++QK TD  + +ID +
Sbjct:   33 ASASMLEPVQVDAYGSHMPLNQVATVSVPEPRLISVQVWDKSMVKAVEKGIVDSNLGLSPATEGQVIRLRIPELNEERRKELVKVAHKYAEAARVAVRHVRRDGLDTIKKLEKAHEISEDDQKRLDQEVQKATDAAIAEIDQL 175          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_PSYA2 (Ribosome-recycling factor OS=Psychrobacter arcticus (strain DSM 17307 / 273-4) GN=frr PE=3 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 5.414e-30
Identity = 61/142 (42.96%), Postives = 99/142 (69.72%), Query Frame = -2
Query:  225 SNPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDS 650
            ++P ML  + V YYGS   L  +A +N  DS +LL+QP+D++ +++I+KAI  +DLG+ P    +VIR+ +P LT E R+++ K+   +AE  +V++RNIRRD +   ++L KEK++SED+    S D+QK+TD+Y++ IDS
Sbjct:   32 AHPGMLSGVMVSYYGSDTPLNQVASVNVEDSRTLLVQPFDRTMVQAIDKAIREADLGLNPMT-ADVIRVPMPALTEETRRDMQKLARGEAENSRVSIRNIRRDMMNDIKELAKEKEISEDDEHRASDDIQKITDKYIETIDS 172          
BLAST of CF832535 vs. ExPASy Swiss-Prot
Match: RRF_NOCSJ (Ribosome-recycling factor OS=Nocardioides sp. (strain BAA-499 / JS614) GN=frr PE=3 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 5.414e-30
Identity = 64/144 (44.44%), Postives = 96/144 (66.67%), Query Frame = -2
Query:  216 NPAMLDKIEVEYYGSPVSLKSIAQINTPDSSSLLIQPYDKSSLKSIEKAIVSSDLGMTPNNDGEVIRLTLPQLTSERRKELSKVVAKQAEEGKVAVRNIRRDALKAYEKLQKEKKLSEDNVKDLSSDLQKLTDEYMKKIDSIYK 647
            NP+M +KI V+YYGSP  L+ +A    P++  +LI PYD+ ++ +I KAI  SDLG+ P +DG+VIR   P+LT ERRKE  K+   +AEEG+VAVRN+RR A +  E+L+K+ ++ +D++      L  LT ++   ID + K
Sbjct:   33 NPSMFNKIVVDYYGSPTPLQQLASFTAPEARIILIAPYDQGAMHNILKAIRDSDLGVNPADDGKVIRCVFPELTEERRKEYIKISRHKAEEGRVAVRNLRRTAKQHLERLEKDGEVGQDDLTGAEKRLDGLTKKHTDAIDEMLK 176          
The following BLAST results are available for this feature:
BLAST of CF832535 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
RRF_BORBZ4.145e-3044.60Ribosome-recycling factor OS=Borrelia burgdorferi ... [more]
RRF_BORBU4.145e-3046.04Ribosome-recycling factor OS=Borrelia burgdorferi ... [more]
RRF_BORAP4.145e-3044.60Ribosome-recycling factor OS=Borrelia afzelii (str... [more]
RRF_VIBF15.414e-3046.85Ribosome-recycling factor OS=Vibrio fischeri (stra... [more]
RRF_TREDE5.414e-3044.06Ribosome-recycling factor OS=Treponema denticola G... [more]
RRF_SALTO5.414e-3042.76Ribosome-recycling factor OS=Salinispora tropica (... [more]
RRF_RHOPT5.414e-3043.36Ribosome-recycling factor OS=Rhodopseudomonas palu... [more]
RRF_RHOPA5.414e-3043.36Ribosome-recycling factor OS=Rhodopseudomonas palu... [more]
RRF_PSYA25.414e-3042.96Ribosome-recycling factor OS=Psychrobacter arcticu... [more]
RRF_NOCSJ5.414e-3044.44Ribosome-recycling factor OS=Nocardioides sp. (str... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_02A06_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa02A06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF832535 ID=CF832535; Name=CF832535; organism=Citrus sinensis; type=EST; length=651bp
TTTTTTTTTTTTTTTTTACAAAGAGTCTAGAATAACTAATTTCCCCCTTT
CATTTCATAACAAAATATCCACCCGTGTCTCCCATTATTATCAAAAAATG
CCATTCTATGGGCAAACCAAAAAATAAAGAGTGCATTCTGAGATTCACTA
GAGAGCTCACCCTTACATTTGTGTGTAAACAATCATTAAACCTTCAACAA
CTCCTTCTCTTTCTGTTTGTAGATGCTATCAATCTTCTTCATATACTCAT
CTGTTAGTTTCTGTAAATCACTTGACAAGTCCTTCACATTGTCTTCAGAG
AGCTTCTTCTCCTTCTGAAGTTTTTCATAAGCTTTTAACGCATCTCTTCT
TATATTCCTAACTGCCACCTTCCCTTCTTCAGCCTGTTTGGCCACAACCT
TTGACAGTTCCTTCCTCCTTTCGGATGTCAATTGAGGAAGGGTTAACCGT
ATGACTTCACCATCATTGTTTGGGGTCATGCCAAGATCAGAGCTCACTAT
GGCCTTCTCTATAGACTTTAAGCTGGATTTGTCATATGGTTGAATCAATA
GAGAACTTGAATCAGGGGTATTGATTTGAGCTATGCTCTTCAAACTTACT
GGGCTTCCATAGTATTCCACCTCAATCTTATCTAGCATAGCTGGATTCGA
T
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