CF834295

Overview
NameCF834295
Unique NameCF834295
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length598
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_FRAT1 (S-adenosylmethionine synthetase OS=Francisella tularensis subsp. tularensis (strain FSC 198) GN=metK PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 1.581e-30
Identity = 68/119 (57.14%), Postives = 87/119 (73.11%), Query Frame = -2 
Query:  235 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWEVV 591
            HGG AF GKDP KVDR+GAY+ R  AK+IVA+GLA +C VQV+YAIGV +P+SL V+++GTGKI D +I K+V E  D R G +  NLDL R     + K + YGHFGR+ P+FTWE +
Sbjct:  259 HGGGAFSGKDPSKVDRSGAYMGRYIAKNIVAAGLADKCEVQVAYAIGVAKPVSLMVNTFGTGKITDNQIEKLVAEVFDLRVGKIIENLDLLR---PIYRKTSNYGHFGRELPEFTWEKI 374
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_FERNB (S-adenosylmethionine synthetase OS=Fervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1) GN=metK PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 1.581e-30
Identity = 65/126 (51.59%), Postives = 91/126 (72.22%), Query Frame = -2 
Query:  223 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE---VVKPLK 591
            HGG AF GKDP KVDR+  Y  R  AK++VA+GLA + ++QV+YAIGV  P+S+ ++++GT K+ ++++ K+V+E  DFRPG +   LDL R     + K AAYGHFGR+DPDFTWE   +V+ LK
Sbjct:  269 HGGGAFSGKDPTKVDRSAHYFARYVAKNVVAAGLADKFMIQVAYAIGVARPVSIMINTFGTAKVDEEKLRKVVEEIFDFRPGAIIKKLDLLR---PIYRKTAAYGHFGRNDPDFTWERTDMVRELK 391
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_COXBU (S-adenosylmethionine synthetase OS=Coxiella burnetii GN=metK PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 1.581e-30
Identity = 65/117 (55.56%), Postives = 82/117 (70.09%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG  F GKDP KVDR+GAY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GTGKI +  I +++KE  D RPG +   L+L R     +   A YGHFGR++P+FTWE
Sbjct:  259 HGGGCFSGKDPSKVDRSGAYAARYVAKNIVAGGLADRCEIQVSYAIGVAEPTSISVETFGTGKIDEVRIQQLIKEHFDLRPGKIIEELNLLR---SIYKATATYGHFGREEPEFTWE 372
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_COXBR (S-adenosylmethionine synthetase OS=Coxiella burnetii (strain RSA 331 / Henzerling II) GN=metK PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 1.581e-30
Identity = 65/117 (55.56%), Postives = 82/117 (70.09%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG  F GKDP KVDR+GAY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GTGKI +  I +++KE  D RPG +   L+L R     +   A YGHFGR++P+FTWE
Sbjct:  259 HGGGCFSGKDPSKVDRSGAYAARYVAKNIVAGGLADRCEIQVSYAIGVAEPTSISVETFGTGKIDEVRIQQLIKEHFDLRPGKIIEELNLLR---SIYKATATYGHFGREEPEFTWE 372
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_COXB2 (S-adenosylmethionine synthetase OS=Coxiella burnetii (strain CbuG_Q212) GN=metK PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 1.581e-30
Identity = 65/117 (55.56%), Postives = 82/117 (70.09%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG  F GKDP KVDR+GAY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GTGKI +  I +++KE  D RPG +   L+L R     +   A YGHFGR++P+FTWE
Sbjct:  259 HGGGCFSGKDPSKVDRSGAYAARYVAKNIVAGGLADRCEIQVSYAIGVAEPTSISVETFGTGKIDEVRIQQLIKEHFDLRPGKIIEELNLLR---SIYKATATYGHFGREEPEFTWE 372
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_COXB1 (S-adenosylmethionine synthetase OS=Coxiella burnetii (strain CbuK_Q154) GN=metK PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 1.581e-30
Identity = 65/117 (55.56%), Postives = 82/117 (70.09%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG  F GKDP KVDR+GAY  R  AK+IVA GLA RC +QVSYAIGV EP S+ V+++GTGKI +  I +++KE  D RPG +   L+L R     +   A YGHFGR++P+FTWE
Sbjct:  259 HGGGCFSGKDPSKVDRSGAYAARYVAKNIVAGGLADRCEIQVSYAIGVAEPTSISVETFGTGKIDEVRIQQLIKEHFDLRPGKIIEELNLLR---SIYKATATYGHFGREEPEFTWE 372
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_ACIF5 (S-adenosylmethionine synthetase OS=Acidithiobacillus ferrooxidans (strain ATCC 53993) GN=metK PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 1.581e-30
Identity = 68/119 (57.14%), Postives = 85/119 (71.43%), Query Frame = -2 
Query:  241 GAHGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 597
            G+HGG AF GKDP KVDR+ +Y  R  AK+IVA+GLA RC VQV+YAIGV +P+SL VD++GTG I D  I  +V+E  D RP  +   LDL R     + K AAYGHFGR++P+FTWE
Sbjct:  259 GSHGGGAFSGKDPSKVDRSSSYAGRYVAKNIVAAGLARRCEVQVAYAIGVSQPVSLMVDTFGTGVIDDDRIAALVQEHFDLRPKGIIQMLDLLR---PIYAKTAAYGHFGREEPEFTWE 374
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_ACIF2 (S-adenosylmethionine synthetase OS=Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) GN=metK PE=3 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 1.581e-30
Identity = 68/119 (57.14%), Postives = 85/119 (71.43%), Query Frame = -2 
Query:  241 GAHGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 597
            G+HGG AF GKDP KVDR+ +Y  R  AK+IVA+GLA RC VQV+YAIGV +P+SL VD++GTG I D  I  +V+E  D RP  +   LDL R     + K AAYGHFGR++P+FTWE
Sbjct:  259 GSHGGGAFSGKDPSKVDRSSSYAGRYVAKNIVAAGLARRCEVQVAYAIGVSQPVSLMVDTFGTGVIDDDRIAALVQEHFDLRPKGIIQMLDLLR---PIYAKTAAYGHFGREEPEFTWE 374
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_NITEU (S-adenosylmethionine synthetase OS=Nitrosomonas europaea GN=metK PE=3 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 2.065e-30
Identity = 66/117 (56.41%), Postives = 83/117 (70.94%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA +C VQV+YAIGV +P+SL V ++GTGKIPD ++ +++    D RP  +   LDL R   G   K AAYGHFGR++P FTWE
Sbjct:  260 HGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLARKCEVQVAYAIGVAKPVSLMVQTFGTGKIPDGKLAELIARHFDLRPRAIIHELDLLRPIYG---KTAAYGHFGREEPSFTWE 373
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_DESAD (S-adenosylmethionine synthetase OS=Desulfovibrio salexigens (strain ATCC 14822 / DSM 2638 / NCIB 8403 / VKM B-1763) GN=metK PE=3 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 2.065e-30
Identity = 68/120 (56.67%), Postives = 87/120 (72.50%), Query Frame = -2 
Query:  241 GAHGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAA-YGHFGRDDPDFTWE 597
            G HGG AF GKDP KVDR+GAY+ R  AK+IVA+GLA R  VQV+YAIGV EP+S+   S+GTG++ D+ + K VKE  D RP  ++  LDL+R       KP+A YGHFGR++P+FTWE
Sbjct:  260 GNHGGGAFSGKDPSKVDRSGAYMARYIAKNIVAAGLAERAEVQVAYAIGVAEPVSVLATSHGTGEVSDETLTKAVKEVFDLRPWYISERLDLRR----PIYKPSACYGHFGRNNPNFTWE 375
The following BLAST results are available for this feature:
BLAST of CF834295 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_FRAT11.581e-3057.14S-adenosylmethionine synthetase OS=Francisella tul... [more]
METK_FERNB1.581e-3051.59S-adenosylmethionine synthetase OS=Fervidobacteriu... [more]
METK_COXBU1.581e-3055.56S-adenosylmethionine synthetase OS=Coxiella burnet... [more]
METK_COXBR1.581e-3055.56S-adenosylmethionine synthetase OS=Coxiella burnet... [more]
METK_COXB21.581e-3055.56S-adenosylmethionine synthetase OS=Coxiella burnet... [more]
METK_COXB11.581e-3055.56S-adenosylmethionine synthetase OS=Coxiella burnet... [more]
METK_ACIF51.581e-3057.14S-adenosylmethionine synthetase OS=Acidithiobacill... [more]
METK_ACIF21.581e-3057.14S-adenosylmethionine synthetase OS=Acidithiobacill... [more]
METK_NITEU2.065e-3056.41S-adenosylmethionine synthetase OS=Nitrosomonas eu... [more]
METK_DESAD2.065e-3056.67S-adenosylmethionine synthetase OS=Desulfovibrio s... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_05E14_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa05E14, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF834295 ID=CF834295; Name=CF834295; organism=Citrus sinensis; type=EST; length=598bp
GGAACATTAAACCAAAACCAAAAAAGACCTCGGGGAAAGAAGAAAAAGAA
AGGAATTGAATGGGGAACATGTATGAGTTCCGAACACAGGAGATTTGCTT
AGTATTACAAAGCAGCAGCAAGATAATATTCAAATTATTCATCCACTACC
AATAGAAGCAAACTTGAACAGGAGGGATAGTTTGCACTTTTCAACTCAAA
TTTAAGCTTGAGGCTTTTCCCACTTGAGGGGCTTCACAACTTCCCAAGTG
AAGTCTGGGTCATCCCTGCCAAAGTGTCCATAGGCAGCAGGCTTCAAGAA
CCTGCCATTACCGCCCCTTTTGAGATCCAAATTGATGGTCATCATTCCTG
GCCTGAAATCAGAGCTTTCCTTCACAATTTTGAGGATCTCCTTGTCAGGA
ATCTTGCCGGTTCCATATGAGTCCACAAAAAGAGACAATGGCTCAGGCAC
ACCTATGGCATACGAGACTTGGACAATGCACCTGGGAGCAAGCCCGCTTG
CAACAATGCTCTTAGCAGCCTGCCTAACAATGTATGCACCAGTCCTATCC
ACCTTAGGAGGGTCTTTTCCAAAGAAAGCACAGCCACCATGGGCTCCC
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