CF834295

Overview
NameCF834295
Unique NameCF834295
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length598
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_FRASN (S-adenosylmethionine synthetase OS=Frankia sp. (strain EAN1pec) GN=metK PE=3 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 2.283e-29
Identity = 64/117 (54.70%), Postives = 82/117 (70.09%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY +R  AK++VA+GLA RC VQV+YAIG   P+ LFV+++GTGK+PD +I   V +  D RP  +  +LDL R     + + AAYGHFGR + DFTWE
Sbjct:  271 HGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLASRCEVQVAYAIGKAHPVGLFVETFGTGKVPDAQIQDAVTQVFDLRPAAIVRDLDLLR---PIYAQTAAYGHFGRPELDFTWE 384
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_BURVG (S-adenosylmethionine synthetase OS=Burkholderia vietnamiensis (strain G4 / LMG 22486) GN=metK PE=3 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 2.283e-29
Identity = 65/117 (55.56%), Postives = 84/117 (71.79%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA R ++QVSYAIGV EP S+ V+++GTG++ D  I K+V+E  D RP  +   LDL R     + K AAYGHFGR++P+F+WE
Sbjct:  261 HGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGTGRVSDAVITKLVREHFDLRPKGIIKMLDLLR---PIYEKTAAYGHFGREEPEFSWE 374
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_BURS3 (S-adenosylmethionine synthetase OS=Burkholderia sp. (strain 383) GN=metK PE=3 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 2.283e-29
Identity = 65/117 (55.56%), Postives = 84/117 (71.79%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA R ++QVSYAIGV EP S+ V+++GTG++ D  I K+V+E  D RP  +   LDL R     + K AAYGHFGR++P+F+WE
Sbjct:  261 HGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGTGRVSDAVITKLVREHFDLRPKGIIKMLDLLR---PIYEKTAAYGHFGREEPEFSWE 374
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_BURCM (S-adenosylmethionine synthetase OS=Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) GN=metK PE=3 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 2.283e-29
Identity = 65/117 (55.56%), Postives = 84/117 (71.79%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA R ++QVSYAIGV EP S+ V+++GTG++ D  I K+V+E  D RP  +   LDL R     + K AAYGHFGR++P+F+WE
Sbjct:  261 HGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGTGRVSDAVITKLVREHFDLRPKGIIKMLDLLR---PIYEKTAAYGHFGREEPEFSWE 374
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_BURCJ (S-adenosylmethionine synthetase OS=Burkholderia cepacia (strain J2315 / LMG 16656) GN=metK PE=3 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 2.283e-29
Identity = 65/117 (55.56%), Postives = 84/117 (71.79%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA R ++QVSYAIGV EP S+ V+++GTG++ D  I K+V+E  D RP  +   LDL R     + K AAYGHFGR++P+F+WE
Sbjct:  261 HGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGTGRVSDAVITKLVREHFDLRPKGIIKMLDLLR---PIYEKTAAYGHFGREEPEFSWE 374
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_BURCH (S-adenosylmethionine synthetase OS=Burkholderia cenocepacia (strain HI2424) GN=metK PE=3 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 2.283e-29
Identity = 65/117 (55.56%), Postives = 84/117 (71.79%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA R ++QVSYAIGV EP S+ V+++GTG++ D  I K+V+E  D RP  +   LDL R     + K AAYGHFGR++P+F+WE
Sbjct:  261 HGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGTGRVSDAVITKLVREHFDLRPKGIIKMLDLLR---PIYEKTAAYGHFGREEPEFSWE 374
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_BURCC (S-adenosylmethionine synthetase OS=Burkholderia cenocepacia (strain MC0-3) GN=metK PE=3 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 2.283e-29
Identity = 65/117 (55.56%), Postives = 84/117 (71.79%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA R ++QVSYAIGV EP S+ V+++GTG++ D  I K+V+E  D RP  +   LDL R     + K AAYGHFGR++P+F+WE
Sbjct:  261 HGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGTGRVSDAVITKLVREHFDLRPKGIIKMLDLLR---PIYEKTAAYGHFGREEPEFSWE 374
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_BURCA (S-adenosylmethionine synthetase OS=Burkholderia cenocepacia (strain AU 1054) GN=metK PE=3 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 2.283e-29
Identity = 65/117 (55.56%), Postives = 84/117 (71.79%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA R ++QVSYAIGV EP S+ V+++GTG++ D  I K+V+E  D RP  +   LDL R     + K AAYGHFGR++P+F+WE
Sbjct:  261 HGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGTGRVSDAVITKLVREHFDLRPKGIIKMLDLLR---PIYEKTAAYGHFGREEPEFSWE 374
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_BURA4 (S-adenosylmethionine synthetase OS=Burkholderia ambifaria (strain MC40-6) GN=metK PE=3 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 2.283e-29
Identity = 65/117 (55.56%), Postives = 84/117 (71.79%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA R ++QVSYAIGV EP S+ V+++GTG++ D  I K+V+E  D RP  +   LDL R     + K AAYGHFGR++P+F+WE
Sbjct:  261 HGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLASRALIQVSYAIGVAEPTSVMVNTFGTGRVSDAVITKLVREHFDLRPKGIIKMLDLLR---PIYEKTAAYGHFGREEPEFSWE 374
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_THENN (S-adenosylmethionine synthetase OS=Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E) GN=metK PE=3 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 2.982e-29
Identity = 59/117 (50.43%), Postives = 87/117 (74.36%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+  Y+ R  AK++VA+GLA + ++Q+SYAIGV +P+S+ +D++GT K+ ++++LK++ E  DFRPG +   L+L R     + K AAYGHFGR++ +FTWE
Sbjct:  268 HGGGAFSGKDPTKVDRSAHYMARYVAKNVVAAGLADKFLIQLSYAIGVAKPVSILIDTFGTAKVDEEKLLKVITEIFDFRPGAIIKKLNLLR---PIYRKTAAYGHFGRNEEEFTWE 381
The following BLAST results are available for this feature:
BLAST of CF834295 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_FRASN2.283e-2954.70S-adenosylmethionine synthetase OS=Frankia sp. (st... [more]
METK_BURVG2.283e-2955.56S-adenosylmethionine synthetase OS=Burkholderia vi... [more]
METK_BURS32.283e-2955.56S-adenosylmethionine synthetase OS=Burkholderia sp... [more]
METK_BURCM2.283e-2955.56S-adenosylmethionine synthetase OS=Burkholderia am... [more]
METK_BURCJ2.283e-2955.56S-adenosylmethionine synthetase OS=Burkholderia ce... [more]
METK_BURCH2.283e-2955.56S-adenosylmethionine synthetase OS=Burkholderia ce... [more]
METK_BURCC2.283e-2955.56S-adenosylmethionine synthetase OS=Burkholderia ce... [more]
METK_BURCA2.283e-2955.56S-adenosylmethionine synthetase OS=Burkholderia ce... [more]
METK_BURA42.283e-2955.56S-adenosylmethionine synthetase OS=Burkholderia am... [more]
METK_THENN2.982e-2950.43S-adenosylmethionine synthetase OS=Thermotoga neap... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_05E14_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa05E14, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF834295 ID=CF834295; Name=CF834295; organism=Citrus sinensis; type=EST; length=598bp
GGAACATTAAACCAAAACCAAAAAAGACCTCGGGGAAAGAAGAAAAAGAA
AGGAATTGAATGGGGAACATGTATGAGTTCCGAACACAGGAGATTTGCTT
AGTATTACAAAGCAGCAGCAAGATAATATTCAAATTATTCATCCACTACC
AATAGAAGCAAACTTGAACAGGAGGGATAGTTTGCACTTTTCAACTCAAA
TTTAAGCTTGAGGCTTTTCCCACTTGAGGGGCTTCACAACTTCCCAAGTG
AAGTCTGGGTCATCCCTGCCAAAGTGTCCATAGGCAGCAGGCTTCAAGAA
CCTGCCATTACCGCCCCTTTTGAGATCCAAATTGATGGTCATCATTCCTG
GCCTGAAATCAGAGCTTTCCTTCACAATTTTGAGGATCTCCTTGTCAGGA
ATCTTGCCGGTTCCATATGAGTCCACAAAAAGAGACAATGGCTCAGGCAC
ACCTATGGCATACGAGACTTGGACAATGCACCTGGGAGCAAGCCCGCTTG
CAACAATGCTCTTAGCAGCCTGCCTAACAATGTATGCACCAGTCCTATCC
ACCTTAGGAGGGTCTTTTCCAAAGAAAGCACAGCCACCATGGGCTCCC
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