CF834295

Overview
NameCF834295
Unique NameCF834295
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length598
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_SHIFL (S-adenosylmethionine synthetase OS=Shigella flexneri GN=metK PE=3 SV=2)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 61/117 (52.14%), Postives = 80/117 (68.38%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E  D RP  +   LDL    +  + + AAYGHFGR+   F WE
Sbjct:  258 HGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_SHIF8 (S-adenosylmethionine synthetase OS=Shigella flexneri serotype 5b (strain 8401) GN=metK PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 61/117 (52.14%), Postives = 80/117 (68.38%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E  D RP  +   LDL    +  + + AAYGHFGR+   F WE
Sbjct:  258 HGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_SHIDS (S-adenosylmethionine synthetase OS=Shigella dysenteriae serotype 1 (strain Sd197) GN=metK PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 61/117 (52.14%), Postives = 80/117 (68.38%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E  D RP  +   LDL    +  + + AAYGHFGR+   F WE
Sbjct:  258 HGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_SHIBS (S-adenosylmethionine synthetase OS=Shigella boydii serotype 4 (strain Sb227) GN=metK PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 61/117 (52.14%), Postives = 80/117 (68.38%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E  D RP  +   LDL    +  + + AAYGHFGR+   F WE
Sbjct:  258 HGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_SHIB3 (S-adenosylmethionine synthetase OS=Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) GN=metK PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 61/117 (52.14%), Postives = 80/117 (68.38%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E  D RP  +   LDL    +  + + AAYGHFGR+   F WE
Sbjct:  258 HGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_PSYA2 (S-adenosylmethionine synthetase OS=Psychrobacter arcticus (strain DSM 17307 / 273-4) GN=metK PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 60/121 (49.59%), Postives = 84/121 (69.42%), Query Frame = -2 
Query:  235 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPD--FTWEVV 591
            HGG AF GKDP KVDR+ AY VR  AK+IVA+G+A RC VQVSYAIGV EP S+ ++++GT KI + EI+++++   D RP  +T  L+L +     + + A++GHFGR   +  FTWE +
Sbjct:  259 HGGGAFSGKDPSKVDRSAAYAVRYVAKNIVAAGIADRCEVQVSYAIGVAEPTSISINTFGTNKISNDEIIRLIRTHFDLRPYGITRMLNLLQ---PMYQQTASFGHFGRQGSETAFTWEKI 376
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_MYCPA (S-adenosylmethionine synthetase OS=Mycobacterium paratuberculosis GN=metK PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 62/117 (52.99%), Postives = 78/117 (66.67%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY +R  AK+IVA+GLA R  VQV+YAIG   P+ LFV+++GT  +   +I KIV E  D RPG +  +LDL R     + + AAYGHFGR D +  WE
Sbjct:  278 HGGGAFSGKDPSKVDRSAAYAMRWVAKNIVAAGLAERVEVQVAYAIGKAAPVGLFVETFGTATVDPVKIEKIVPEVFDLRPGAIIRDLDLLR---PIYAQTAAYGHFGRTDVELPWE 391
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_MARAV (S-adenosylmethionine synthetase OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=metK PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 63/128 (49.22%), Postives = 83/128 (64.84%), Query Frame = -2 
Query:  214 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFT--WEVVKPLKWEK 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA +C +QVSYAIGV +P S+ ++++GTGKI D +I+ +V+   D RP  +T  LDL    +  +   AAYGHFGRD  + T   +      WEK
Sbjct:  259 HGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLAEKCEIQVSYAIGVAQPTSISLNTFGTGKISDDKIIDLVRAHFDLRPYAITNMLDLL---HPMYRATAAYGHFGRDPYEMTVGGKTFTAFPWEK 383
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_KLEP7 (S-adenosylmethionine synthetase OS=Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) GN=metK PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 61/117 (52.14%), Postives = 80/117 (68.38%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E  D RP  +   LDL    +  + + AAYGHFGR+   F WE
Sbjct:  258 HGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_KLEP3 (S-adenosylmethionine synthetase OS=Klebsiella pneumoniae (strain 342) GN=metK PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 61/117 (52.14%), Postives = 80/117 (68.38%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E  D RP  +   LDL    +  + + AAYGHFGR+   F WE
Sbjct:  258 HGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
The following BLAST results are available for this feature:
BLAST of CF834295 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_SHIFL1.172e-2552.14S-adenosylmethionine synthetase OS=Shigella flexne... [more]
METK_SHIF81.172e-2552.14S-adenosylmethionine synthetase OS=Shigella flexne... [more]
METK_SHIDS1.172e-2552.14S-adenosylmethionine synthetase OS=Shigella dysent... [more]
METK_SHIBS1.172e-2552.14S-adenosylmethionine synthetase OS=Shigella boydii... [more]
METK_SHIB31.172e-2552.14S-adenosylmethionine synthetase OS=Shigella boydii... [more]
METK_PSYA21.172e-2549.59S-adenosylmethionine synthetase OS=Psychrobacter a... [more]
METK_MYCPA1.172e-2552.99S-adenosylmethionine synthetase OS=Mycobacterium p... [more]
METK_MARAV1.172e-2549.22S-adenosylmethionine synthetase OS=Marinobacter aq... [more]
METK_KLEP71.172e-2552.14S-adenosylmethionine synthetase OS=Klebsiella pneu... [more]
METK_KLEP31.172e-2552.14S-adenosylmethionine synthetase OS=Klebsiella pneu... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_05E14_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa05E14, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF834295 ID=CF834295; Name=CF834295; organism=Citrus sinensis; type=EST; length=598bp
GGAACATTAAACCAAAACCAAAAAAGACCTCGGGGAAAGAAGAAAAAGAA
AGGAATTGAATGGGGAACATGTATGAGTTCCGAACACAGGAGATTTGCTT
AGTATTACAAAGCAGCAGCAAGATAATATTCAAATTATTCATCCACTACC
AATAGAAGCAAACTTGAACAGGAGGGATAGTTTGCACTTTTCAACTCAAA
TTTAAGCTTGAGGCTTTTCCCACTTGAGGGGCTTCACAACTTCCCAAGTG
AAGTCTGGGTCATCCCTGCCAAAGTGTCCATAGGCAGCAGGCTTCAAGAA
CCTGCCATTACCGCCCCTTTTGAGATCCAAATTGATGGTCATCATTCCTG
GCCTGAAATCAGAGCTTTCCTTCACAATTTTGAGGATCTCCTTGTCAGGA
ATCTTGCCGGTTCCATATGAGTCCACAAAAAGAGACAATGGCTCAGGCAC
ACCTATGGCATACGAGACTTGGACAATGCACCTGGGAGCAAGCCCGCTTG
CAACAATGCTCTTAGCAGCCTGCCTAACAATGTATGCACCAGTCCTATCC
ACCTTAGGAGGGTCTTTTCCAAAGAAAGCACAGCCACCATGGGCTCCC
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