CF834295

Overview
NameCF834295
Unique NameCF834295
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length598
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_ECOBW (S-adenosylmethionine synthetase OS=Escherichia coli (strain K12 / BW2952) GN=metK PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 61/117 (52.14%), Postives = 80/117 (68.38%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E  D RP  +   LDL    +  + + AAYGHFGR+   F WE
Sbjct:  258 HGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_ECO8A (S-adenosylmethionine synthetase OS=Escherichia coli O8 (strain IAI1) GN=metK PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 61/117 (52.14%), Postives = 80/117 (68.38%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E  D RP  +   LDL    +  + + AAYGHFGR+   F WE
Sbjct:  258 HGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_ECO7I (S-adenosylmethionine synthetase OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=metK PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 61/117 (52.14%), Postives = 80/117 (68.38%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E  D RP  +   LDL    +  + + AAYGHFGR+   F WE
Sbjct:  258 HGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_ECO5E (S-adenosylmethionine synthetase OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=metK PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 61/117 (52.14%), Postives = 80/117 (68.38%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E  D RP  +   LDL    +  + + AAYGHFGR+   F WE
Sbjct:  258 HGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_ECO57 (S-adenosylmethionine synthetase OS=Escherichia coli O157:H7 GN=metK PE=3 SV=2)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 61/117 (52.14%), Postives = 80/117 (68.38%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E  D RP  +   LDL    +  + + AAYGHFGR+   F WE
Sbjct:  258 HGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_ECO55 (S-adenosylmethionine synthetase OS=Escherichia coli (strain 55989 / EAEC) GN=metK PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 61/117 (52.14%), Postives = 80/117 (68.38%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E  D RP  +   LDL    +  + + AAYGHFGR+   F WE
Sbjct:  258 HGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_ECO27 (S-adenosylmethionine synthetase OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=metK PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 61/117 (52.14%), Postives = 80/117 (68.38%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E  D RP  +   LDL    +  + + AAYGHFGR+   F WE
Sbjct:  258 HGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_ECO24 (S-adenosylmethionine synthetase OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=metK PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 61/117 (52.14%), Postives = 80/117 (68.38%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+ AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E  D RP  +   LDL    +  + + AAYGHFGR+   F WE
Sbjct:  258 HGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_CORJK (S-adenosylmethionine synthetase OS=Corynebacterium jeikeium (strain K411) GN=metK PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 61/117 (52.14%), Postives = 78/117 (66.67%), Query Frame = -2 
Query:  241 HGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 591
            HGG AF GKDP KVDR+GAY +R  AK+IVA+GLA R  VQV+YAIG   P+ L+V+++GT K P + I   V++  D RP  +   LDLKR     + + +AYGHFGR D D  WE
Sbjct:  279 HGGGAFSGKDPSKVDRSGAYAMRWVAKNIVAAGLADRAEVQVAYAIGRATPVGLYVETFGTEKAPVEAIQAAVRQVFDLRPAAILQELDLKR---PIYAQTSAYGHFGRTDLDLPWE 392
[Close]
BLAST of CF834295 vs. ExPASy Swiss-Prot
Match: METK_CLONN (S-adenosylmethionine synthetase OS=Clostridium novyi (strain NT) GN=metK PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.172e-25
Identity = 59/119 (49.58%), Postives = 81/119 (68.07%), Query Frame = -2 
Query:  241 GAHGGCAFFGKDPPKVDRTGAYIVRQAAKSIVASGLAPRCIVQVSYAIGVPEPLSLFVDSYGTGKIPDKEILKIVKESSDFRPGMMTINLDLKRGGNGRFLKPAAYGHFGRDDPDFTWE 597
            G HGG AF GKDP KVDR+ AY  R  AK++VA+G+A +  + ++YAIGV  P+SL V+++GT KI D +I+ IV +  D RPG +  +L+LKR     + + AAYGHFGR D +  WE
Sbjct:  264 GRHGGGAFSGKDPTKVDRSAAYAARWVAKNLVAAGVADKIEIGLAYAIGVARPVSLLVNTFGTEKIEDSKIVDIVNKVFDLRPGAIIRDLELKR---PIYRQTAAYGHFGRTDVELPWE 379
The following BLAST results are available for this feature:
BLAST of CF834295 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_ECOBW1.172e-2552.14S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_ECO8A1.172e-2552.14S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_ECO7I1.172e-2552.14S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_ECO5E1.172e-2552.14S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_ECO571.172e-2552.14S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_ECO551.172e-2552.14S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_ECO271.172e-2552.14S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_ECO241.172e-2552.14S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_CORJK1.172e-2552.14S-adenosylmethionine synthetase OS=Corynebacterium... [more]
METK_CLONN1.172e-2549.58S-adenosylmethionine synthetase OS=Clostridium nov... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_05E14_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa05E14, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF834295 ID=CF834295; Name=CF834295; organism=Citrus sinensis; type=EST; length=598bp
GGAACATTAAACCAAAACCAAAAAAGACCTCGGGGAAAGAAGAAAAAGAA
AGGAATTGAATGGGGAACATGTATGAGTTCCGAACACAGGAGATTTGCTT
AGTATTACAAAGCAGCAGCAAGATAATATTCAAATTATTCATCCACTACC
AATAGAAGCAAACTTGAACAGGAGGGATAGTTTGCACTTTTCAACTCAAA
TTTAAGCTTGAGGCTTTTCCCACTTGAGGGGCTTCACAACTTCCCAAGTG
AAGTCTGGGTCATCCCTGCCAAAGTGTCCATAGGCAGCAGGCTTCAAGAA
CCTGCCATTACCGCCCCTTTTGAGATCCAAATTGATGGTCATCATTCCTG
GCCTGAAATCAGAGCTTTCCTTCACAATTTTGAGGATCTCCTTGTCAGGA
ATCTTGCCGGTTCCATATGAGTCCACAAAAAGAGACAATGGCTCAGGCAC
ACCTATGGCATACGAGACTTGGACAATGCACCTGGGAGCAAGCCCGCTTG
CAACAATGCTCTTAGCAGCCTGCCTAACAATGTATGCACCAGTCCTATCC
ACCTTAGGAGGGTCTTTTCCAAAGAAAGCACAGCCACCATGGGCTCCC
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