CF834799

Overview
NameCF834799
Unique NameCF834799
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length702
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig3195 contig Csv1_Contig3195:981..1683. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of CF834799 vs. ExPASy Swiss-Prot
Match: IF4A3_BOVIN (Eukaryotic initiation factor 4A-III OS=Bos taurus GN=EIF4A3 PE=2 SV=3)

HSP 1 Score: 217.624 bits (553), Expect = 5.053e-56
Identity = 103/154 (66.88%), Postives = 128/154 (83.12%), Query Frame = 3
Query:    6 LRRYEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNVVIEELPSNVADLL 467
            + R EWK +TLCDLY+TL ITQ+VIF NT+RKVDWLT+KMR  + TVS+ HGDM Q  R+ IM+EFRSG+SRVLI+TD+ ARG+DV QVSL+INYDLP   E Y+HRIGRSGR+GRKGVAINFV  DD R+L DI+++Y+  I+E+P NVADL+
Sbjct:  258 VEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNRELYIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI 411          
BLAST of CF834799 vs. ExPASy Swiss-Prot
Match: I4A3B_XENLA (Eukaryotic initiation factor 4A-III-B OS=Xenopus laevis GN=eif4a3-B PE=2 SV=1)

HSP 1 Score: 217.624 bits (553), Expect = 5.053e-56
Identity = 103/154 (66.88%), Postives = 128/154 (83.12%), Query Frame = 3
Query:    6 LRRYEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNVVIEELPSNVADLL 467
            + R EWK +TLCDLY+TL ITQ+VIF NT+RKVDWLT+KMR  + TVS+ HGDM Q  R+ IM+EFRSG+SRVLI+TD+ ARG+DV QVSL+INYDLP   E Y+HRIGRSGR+GRKGVAINFV  DD R+L DI+++Y+  I+E+P NVADL+
Sbjct:  261 VEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNRELYIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI 414          
BLAST of CF834799 vs. ExPASy Swiss-Prot
Match: FAL1_AJECN (ATP-dependent RNA helicase FAL1 OS=Ajellomyces capsulata (strain NAm1 / WU24) GN=FAL1 PE=3 SV=1)

HSP 1 Score: 216.468 bits (550), Expect = 1.126e-55
Identity = 103/149 (69.13%), Postives = 125/149 (83.89%), Query Frame = 3
Query:    6 LRRYEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNVVIEELPSN 452
            + + EWK +TLCDLY+TL ITQ+VIF NTRRKVDWLTDKMR  + TVS+ HG+M Q  RD IM++FR G+SRVLI+TD+ ARGIDVQQVSLVINYDLP+  ENY+HRIGRSGRFGRKGVAINFVT +D R+L DI+ +Y+  I+E+P N
Sbjct:  245 VEKEEWKFDTLCDLYDTLTITQAVIFCNTRRKVDWLTDKMREANFTVSSMHGEMPQKERDSIMQDFRQGNSRVLISTDVWARGIDVQQVSLVINYDLPSNRENYIHRIGRSGRFGRKGVAINFVTSEDVRILRDIELYYSTQIDEMPMN 393          
BLAST of CF834799 vs. ExPASy Swiss-Prot
Match: I4A3A_XENLA (Eukaryotic initiation factor 4A-III-A OS=Xenopus laevis GN=eif4a3-A PE=2 SV=1)

HSP 1 Score: 214.927 bits (546), Expect = 3.276e-55
Identity = 102/154 (66.23%), Postives = 127/154 (82.47%), Query Frame = 3
Query:    6 LRRYEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNVVIEELPSNVADLL 467
            + R EWK +TLCDLY+TL ITQ+VIF NT+RKVDWLT+KMR  + TVS+ HGDM Q  R+ IM+EFRSG+SRVLI+TD+ ARG+DV QVSL+INYDLP   E Y+HRIGRSGR+G KGVAINFV  DD R+L DI+++Y+  I+E+P NVADL+
Sbjct:  262 VEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNRELYIHRIGRSGRYGGKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI 415          
BLAST of CF834799 vs. ExPASy Swiss-Prot
Match: FAL1_YARLI (ATP-dependent RNA helicase FAL1 OS=Yarrowia lipolytica GN=FAL1 PE=3 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 2.123e-54
Identity = 101/150 (67.33%), Postives = 121/150 (80.67%), Query Frame = 3
Query:   18 EWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNVVIEELPSNVADLL 467
            EWK +TLCDLY+TL ITQ+VIF NT++KVDWLT +M+  + TV + HGDM Q  RD IM EFRSG SRVLI+TD+ ARGIDVQQVSLVINYDLP   ENY+HRIGRSGRFGRKGVAINF T DD   L DI+++Y+  I+E+P NV D++
Sbjct:  248 EWKFDTLCDLYDTLTITQAVIFCNTKKKVDWLTQQMKDNNFTVCSMHGDMAQKDRDSIMNEFRSGRSRVLISTDVWARGIDVQQVSLVINYDLPPNRENYIHRIGRSGRFGRKGVAINFATNDDITTLRDIEQYYSTQIDEMPVNVTDMM 397          
BLAST of CF834799 vs. ExPASy Swiss-Prot
Match: FAL1_USTMA (ATP-dependent RNA helicase FAL1 OS=Ustilago maydis GN=FAL1 PE=3 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 2.123e-54
Identity = 101/154 (65.58%), Postives = 123/154 (79.87%), Query Frame = 3
Query:    6 LRRYEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNVVIEELPSNVADLL 467
            + + EWK +TLCDLY+TL ITQ+VIF NTRRKVDWL+ KM+  +  VS+ HG+M Q  RD +M EFR GSSRVLITTD+ ARGID+  +SLVINYDLPT  ENY+HRIGRSGRFGRKGVAINFVT DD R L DI++FY+  I+E+P  + D+L
Sbjct:  244 VEKEEWKFDTLCDLYDTLTITQAVIFCNTRRKVDWLSAKMKENNFQVSSMHGEMQQKERDEVMAEFRQGSSRVLITTDVWARGIDIANISLVINYDLPTNRENYIHRIGRSGRFGRKGVAINFVTVDDVRTLRDIEQFYSTQIDEMPVKLEDML 397          
BLAST of CF834799 vs. ExPASy Swiss-Prot
Match: IF4A_AJECN (ATP-dependent RNA helicase eIF4A OS=Ajellomyces capsulata (strain NAm1 / WU24) GN=TIF1 PE=3 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 1.797e-53
Identity = 101/128 (78.91%), Postives = 112/128 (87.50%), Query Frame = 3
Query:    6 LRRYEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDD 389
            + + EWKL+TL DLYET+ ITQ+VIF NTRRKVDWLTDK+ +RD TVSA HGDM+Q  RD+IM+EFRSGSSRVLI TDLLARGIDVQQVSLVINYDLP   ENY+HRIGR GRFGRKGVAINFVT DD
Sbjct:  245 VEKEEWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMEQQQRDVIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADD 372          
BLAST of CF834799 vs. ExPASy Swiss-Prot
Match: FAL1_DEBHA (ATP-dependent RNA helicase FAL1 OS=Debaryomyces hansenii GN=FAL1 PE=3 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 2.347e-53
Identity = 99/150 (66.00%), Postives = 125/150 (83.33%), Query Frame = 3
Query:   18 EWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNVVIEELPSNVADLL 467
            +WK +TLCDLY++L ITQ+VIF NT+ KV+WLTD+M+  + TV A HGDM Q+ RD IM +FR+G+SRVLI+TD+ ARGIDVQQVSLVINYDLPT  ENY+HRIGRSGRFGRKGVAIN VT++D   L DI++FY + I+E+P NV D++
Sbjct:  250 DWKFDTLCDLYDSLTITQAVIFCNTKVKVNWLTDQMKKANFTVVAMHGDMKQDERDSIMNDFRTGNSRVLISTDVWARGIDVQQVSLVINYDLPTDKENYVHRIGRSGRFGRKGVAINLVTKEDVDELRDIERFYRIRIKEMPVNVNDIM 399          
BLAST of CF834799 vs. ExPASy Swiss-Prot
Match: IF4A_YARLI (ATP-dependent RNA helicase eIF4A OS=Yarrowia lipolytica GN=TIF1 PE=3 SV=1)

HSP 1 Score: 207.994 bits (528), Expect = 4.004e-53
Identity = 97/149 (65.10%), Postives = 125/149 (83.89%), Query Frame = 3
Query:   21 WKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNVVIEELPSNVADLL 467
            +K E LCDLYET+ ++Q+VIF NTRRKVD+LT  +   D TVS+ HG+ +Q+ RD+IM+ FR+GSSR+LITTDLLARGIDVQQVSLVIN+DLP+  ENY+HRIGR GRFGRKGVAINFVT +D  ML ++++FY+  I E+P+N+ADL+
Sbjct:  247 YKFEVLCDLYETINVSQAVIFCNTRRKVDYLTQALTEADFTVSSMHGETEQSQRDVIMKAFRTGSSRILITTDLLARGIDVQQVSLVINFDLPSNRENYIHRIGRGGRFGRKGVAINFVTSEDHGMLKELERFYSTEIVEMPTNIADLI 395          
BLAST of CF834799 vs. ExPASy Swiss-Prot
Match: FAL1_PICST (ATP-dependent RNA helicase FAL1 OS=Pichia stipitis GN=FAL1 PE=3 SV=1)

HSP 1 Score: 207.994 bits (528), Expect = 4.004e-53
Identity = 96/150 (64.00%), Postives = 123/150 (82.00%), Query Frame = 3
Query:   18 EWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTRDDERMLFDIQKFYNVVIEELPSNVADLL 467
            +WK +TLCDLY+ L ITQ+VIF NT+ KV+WLTD+MR ++ TV + HGDM Q+ RD IM +FR+G+SRVLI+TD+ ARGIDVQQVSLVINYDLPT  ENY+HRIGRSGRFGRKGVAIN +T++D   L D +K+Y+  I E+P N+ D++
Sbjct:  250 DWKFDTLCDLYDNLTITQAVIFCNTKLKVNWLTDQMRKQNFTVVSMHGDMKQDERDSIMNDFRTGNSRVLISTDVWARGIDVQQVSLVINYDLPTDKENYIHRIGRSGRFGRKGVAINLITKEDVATLRDFEKYYSTKIREMPMNINDIM 399          
The following BLAST results are available for this feature:
BLAST of CF834799 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
IF4A3_BOVIN5.053e-5666.88Eukaryotic initiation factor 4A-III OS=Bos taurus ... [more]
I4A3B_XENLA5.053e-5666.88Eukaryotic initiation factor 4A-III-B OS=Xenopus l... [more]
FAL1_AJECN1.126e-5569.13ATP-dependent RNA helicase FAL1 OS=Ajellomyces cap... [more]
I4A3A_XENLA3.276e-5566.23Eukaryotic initiation factor 4A-III-A OS=Xenopus l... [more]
FAL1_YARLI2.123e-5467.33ATP-dependent RNA helicase FAL1 OS=Yarrowia lipoly... [more]
FAL1_USTMA2.123e-5465.58ATP-dependent RNA helicase FAL1 OS=Ustilago maydis... [more]
IF4A_AJECN1.797e-5378.91ATP-dependent RNA helicase eIF4A OS=Ajellomyces ca... [more]
FAL1_DEBHA2.347e-5366.00ATP-dependent RNA helicase FAL1 OS=Debaryomyces ha... [more]
IF4A_YARLI4.004e-5365.10ATP-dependent RNA helicase eIF4A OS=Yarrowia lipol... [more]
FAL1_PICST4.004e-5364.00ATP-dependent RNA helicase FAL1 OS=Pichia stipitis... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06C04_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06C04, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF834799 ID=CF834799; Name=CF834799; organism=Citrus sinensis; type=EST; length=702bp
CAATGTTGAGACGGTATGAATGGAAGCTTGAAACACTCTGTGATCTTTAT
GAGACTCTGGCCATAACCCAGAGTGTCATCTTTGTGAACACTCGGCGAAA
GGTCGACTGGCTTACGGACAAGATGCGAAGCCGTGACCATACAGTCTCTG
CCACTCATGGGGACATGGATCAGAACACCCGAGATATCATTATGCGTGAA
TTTCGCTCTGGTTCTTCTCGTGTTCTCATCACCACTGATCTGTTGGCTCG
TGGTATTGATGTGCAGCAAGTTTCACTTGTCATAAATTATGATCTGCCGA
CTCAACCAGAAAACTACCTTCACCGTATTGGACGAAGTGGTCGATTTGGA
AGAAAGGGTGTTGCCATTAATTTTGTGACAAGGGATGATGAGAGAATGCT
TTTTGATATTCAAAAGTTCTATAACGTGGTCATTGAGGAGCTGCCTTCAA
ACGTTGCTGACCTTCTTTGAGGAGGTTCTGTTTTTGTAAGTTTGTCGAGG
CAAGAAGGGGTTTCCTGATTTTGCAAGTACTGTTTAATGTGGTGGTTCTT
TTCTCCCCTTTTTTTTTTTTTTTGGGTTCTTCTTCCTTTCCGTCTAGTGT
CTTAAATTAATGTTGTTCTCAAACATATCCGACCATTTAATCATATGGGT
CATGCACATTTGCAAGCTGCACTGTGGTGGGTTTATAATTATTGAAATTT
TT
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