CF834867

Overview
NameCF834867
Unique NameCF834867
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length594
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2387 contig Csv1_Contig2387:467..1060. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR1_MOUSE (Pyrroline-5-carboxylate reductase 1, mitochondrial OS=Mus musculus GN=Pycr1 PE=1 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 6.946e-26
Identity = 45/89 (50.56%), Postives = 55/89 (61.80%), Query Frame = -3 
Query:  137 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAA 403
            L GSGPAY F              LPR LA+ L +Q +LGAA M+  S +HP QLKD+V SPGG TI  +H LE  GFR +L+NAV A+
Sbjct:  173 LSGSGPAYAFTALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLDSEQHPSQLKDNVCSPGGATIHALHVLESGGFRSLLINAVEAS 261

HSP 2 Score: 53.1434 bits (126), Expect = 6.946e-26
Identity = 28/58 (48.28%), Postives = 34/58 (58.62%), Query Frame = -2 
Query:  414 RFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 587
            + IR M NTP  V E  TV + G  A  EDG L+ +L GSVG     +E L DA+TGL
Sbjct:  116 KVIRCMTNTPVVVREGVTVYATGTHAQVEDGRLVEQLMGSVGFCTEVEEDLIDAVTGL 173
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR2_MACFA (Pyrroline-5-carboxylate reductase 2 OS=Macaca fascicularis GN=PYCR2 PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.176e-25
Identity = 45/89 (50.56%), Postives = 55/89 (61.80%), Query Frame = -3 
Query:  137 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAA 403
            L GSGPAY F              LPR LA+ L +Q +LGAA M+  S +HP QLKD+V SPGG TI  +H LE  GFR +L+NAV A+
Sbjct:  173 LSGSGPAYAFMALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLDSEQHPCQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEAS 261

HSP 2 Score: 53.9138 bits (128), Expect = 1.176e-25
Identity = 28/58 (48.28%), Postives = 36/58 (62.07%), Query Frame = -2 
Query:  414 RFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 587
            + IR M NTP  V E ATV ++G  A  EDG+L+ +L  SVG     +E L DA+TGL
Sbjct:  116 KVIRCMTNTPVVVREGATVYAMGTHALVEDGQLLEQLMSSVGFCTEVEEDLIDAVTGL 173
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR1_BOVIN (Pyrroline-5-carboxylate reductase 1, mitochondrial OS=Bos taurus GN=PYCR1 PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 1.530e-25
Identity = 45/89 (50.56%), Postives = 55/89 (61.80%), Query Frame = -3 
Query:  137 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAA 403
            L GSGPAY F              LPR LA+ L +Q +LGAA M+  S +HPGQLKD+V SPGG TI  +H LE  GFR +L+ AV A+
Sbjct:  173 LSGSGPAYAFTALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLDSEQHPGQLKDNVCSPGGATIHALHVLESGGFRSLLIKAVEAS 261

HSP 2 Score: 51.9878 bits (123), Expect = 1.530e-25
Identity = 28/58 (48.28%), Postives = 34/58 (58.62%), Query Frame = -2 
Query:  414 RFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 587
            + IR M NTP  V E ATV + G  A  EDG L+ +L  SVG     +E L DA+TGL
Sbjct:  116 KVIRCMTNTPVVVREGATVYATGTHAQVEDGRLLEQLMSSVGFCTEVEEDLIDAVTGL 173
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR2_PONAB (Pyrroline-5-carboxylate reductase 2 OS=Pongo abelii GN=PYCR2 PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 1.992e-25
Identity = 45/89 (50.56%), Postives = 55/89 (61.80%), Query Frame = -3 
Query:  137 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAA 403
            L GSGPAY F              LPR LA+ L +Q +LGAA M+  S +HP QLKD+V SPGG TI  +H LE  GFR +L+NAV A+
Sbjct:  173 LSGSGPAYAFMALDALADGGVKMGLPRRLAIQLGAQALLGAAKMLLDSEQHPCQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEAS 261

HSP 2 Score: 52.7582 bits (125), Expect = 1.992e-25
Identity = 28/58 (48.28%), Postives = 35/58 (60.34%), Query Frame = -2 
Query:  414 RFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 587
            + IR M NTP  V E ATV + G  A  EDG+L+ +L  SVG     +E L DA+TGL
Sbjct:  116 KVIRCMTNTPVVVQEGATVYATGTHALVEDGQLLEQLMSSVGFCTEVEEDLIDAVTGL 173
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR2_HUMAN (Pyrroline-5-carboxylate reductase 2 OS=Homo sapiens GN=PYCR2 PE=1 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 1.992e-25
Identity = 45/89 (50.56%), Postives = 55/89 (61.80%), Query Frame = -3 
Query:  137 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAA 403
            L GSGPAY F              LPR LA+ L +Q +LGAA M+  S +HP QLKD+V SPGG TI  +H LE  GFR +L+NAV A+
Sbjct:  173 LSGSGPAYAFMALDALADGGVKMGLPRRLAIQLGAQALLGAAKMLLDSEQHPCQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEAS 261

HSP 2 Score: 52.7582 bits (125), Expect = 1.992e-25
Identity = 28/58 (48.28%), Postives = 35/58 (60.34%), Query Frame = -2 
Query:  414 RFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 587
            + IR M NTP  V E ATV + G  A  EDG+L+ +L  SVG     +E L DA+TGL
Sbjct:  116 KVIRCMTNTPVVVQEGATVYATGTHALVEDGQLLEQLMSSVGFCTEVEEDLIDAVTGL 173
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR_SYNY3 (Pyrroline-5-carboxylate reductase OS=Synechocystis sp. (strain PCC 6803) GN=proC PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 7.498e-21
Identity = 43/87 (49.43%), Postives = 54/87 (62.07%), Query Frame = -3 
Query:  137 GSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAA 397
            GSGPAY+               LPR +A  LA QTVLG A ++ ++ +HP Q+KD V SPGGTTIAG+  LEK GFR  ++ AV AA
Sbjct:  171 GSGPAYVALMIEALADGGVLAGLPRAIAQKLALQTVLGTAELIKETEEHPAQIKDKVTSPGGTTIAGVAVLEKMGFRSAIIEAVRAA 257

HSP 2 Score: 40.0466 bits (92), Expect = 7.498e-21
Identity = 19/56 (33.93%), Postives = 28/56 (50.00%), Query Frame = -2 
Query:  414 IRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 581
            IR MPNTP+ VG   T ++       +       +F +VG +    E L DA+TG+
Sbjct:  114 IRAMPNTPATVGAGMTAIAANKMVEPDQLAKAKAIFSAVGNVVEVPENLMDAVTGV 169
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR3_DANRE (Pyrroline-5-carboxylate reductase 3 OS=Danio rerio GN=pycrl PE=2 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 1.104e-18
Identity = 40/90 (44.44%), Postives = 54/90 (60.00%), Query Frame = -3 
Query:  134 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAA 403
            L GSG A+++              +P  LA  +A+QT+LGA  ++  SGK P +LK +V +PGGTTI GIH LEK GFR   + AV AA+
Sbjct:  186 LSGSGVAFVYVFAEALADGAVKMGMPSTLARRIAAQTILGAGVLLRDSGKLPAELKAEVCTPGGTTIHGIHALEKGGFRAAAIGAVEAAS 275

HSP 2 Score: 38.5058 bits (88), Expect = 1.104e-18
Identity = 21/56 (37.50%), Postives = 29/56 (51.79%), Query Frame = -2 
Query:  414 IRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 581
            IR+MPN P  + E A ++S G  A E++  L+  L G  G +    E   DA  GL
Sbjct:  131 IRIMPNLPCMLLEGALLLSCGSHAGEQEETLLKTLLGPCGLVEFGPESWIDAHVGL 186
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR3_XENLA (Pyrroline-5-carboxylate reductase 3 OS=Xenopus laevis GN=pycrl PE=2 SV=2)

HSP 1 Score: 79.337 bits (194), Expect = 1.438e-18
Identity = 40/87 (45.98%), Postives = 53/87 (60.92%), Query Frame = -3 
Query:  137 GSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAA 397
            GSG AY++              +P  LA  + +QT+LGA  M+ +S +HP  L+ DV +PGGTTI G+HELEK G R  +MNAV AA
Sbjct:  178 GSGVAYVYTFAEALADGAVKMGMPSALARQIVAQTLLGAGKMLLQSEEHPASLRADVCTPGGTTIFGLHELEKGGLRAAVMNAVEAA 264

HSP 2 Score: 34.2686 bits (77), Expect = 1.438e-18
Identity = 19/59 (32.20%), Postives = 28/59 (47.46%), Query Frame = -2 
Query:  414 SRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 590
            S+ IR+MPN P  + E A V S G  A E + ++   L  + G      +   D  TG+
Sbjct:  118 SKVIRMMPNLPCVLQEGAIVFSRGRCAGEVEADVFESLVRTCGLCVEVPQSCIDIHTGV 176
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR1_BACSU (Pyrroline-5-carboxylate reductase 1 OS=Bacillus subtilis GN=proH PE=3 SV=5)

HSP 1 Score: 67.0106 bits (162), Expect = 2.426e-18
Identity = 34/90 (37.78%), Postives = 53/90 (58.89%), Query Frame = -3 
Query:  134 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAA 403
            + GSGPAY +              L ++L+  + +QT+LGAA M+ ++G+HP  L+D++ SP GTT AG+  L+KSG    +  A+  AA
Sbjct:  186 IAGSGPAYFYYLMEFIEKTGEEAGLDKQLSRSIGAQTLLGAAKMLMETGEHPEILRDNITSPNGTTAAGLQALKKSGGGEAISQAIKHAA 275

HSP 2 Score: 45.8246 bits (107), Expect = 2.426e-18
Identity = 21/56 (37.50%), Postives = 34/56 (60.71%), Query Frame = -2 
Query:  414 IRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 581
            +RVMPNT S +G +AT ++LG   +E+  +L   L G +G+++   E   D  TG+
Sbjct:  131 VRVMPNTSSMIGASATAIALGKYVSEDLKKLAEALLGCMGEVYTIQENQMDIFTGI 186
[Close]
BLAST of CF834867 vs. ExPASy Swiss-Prot
Match: P5CR3_MACFA (Pyrroline-5-carboxylate reductase 3 OS=Macaca fascicularis GN=PYCRL PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 5.346e-18
Identity = 39/89 (43.82%), Postives = 52/89 (58.43%), Query Frame = -3 
Query:  137 LLGSGPAYIFXXXXXXXXXXXXXXLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAA 403
            L GSG A++               +P  LA  +A+QT+LG A M+   G+HP QL+ DV +PGGTTI G+H LE+ G R   M+AV AA
Sbjct:  176 LSGSGVAFVCAFSEALAEGAIKMGMPSSLAHRIAAQTLLGTAKMLLHEGQHPAQLRSDVCTPGGTTIYGLHALEQGGLRAATMSAVEAA 264

HSP 2 Score: 36.5798 bits (83), Expect = 5.346e-18
Identity = 20/60 (33.33%), Postives = 30/60 (50.00%), Query Frame = -2 
Query:  414 HSRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 593
            ++R +RV+PN P  V E A VM+ G      + +L+  L  + G+     E   D  TGL
Sbjct:  117 NTRVLRVLPNLPCVVQEGAIVMARGRHVGSSETKLLQHLLEACGRCEEVPEAYVDIHTGL 176
The following BLAST results are available for this feature:
BLAST of CF834867 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 35
Match NameE-valueIdentityDescription
P5CR1_MOUSE6.946e-2650.56Pyrroline-5-carboxylate reductase 1, mitochondrial... [more]
P5CR2_MACFA1.176e-2550.56Pyrroline-5-carboxylate reductase 2 OS=Macaca fasc... [more]
P5CR1_BOVIN1.530e-2550.56Pyrroline-5-carboxylate reductase 1, mitochondrial... [more]
P5CR2_PONAB1.992e-2550.56Pyrroline-5-carboxylate reductase 2 OS=Pongo abeli... [more]
P5CR2_HUMAN1.992e-2550.56Pyrroline-5-carboxylate reductase 2 OS=Homo sapien... [more]
P5CR_SYNY37.498e-2149.43Pyrroline-5-carboxylate reductase OS=Synechocystis... [more]
P5CR3_DANRE1.104e-1844.44Pyrroline-5-carboxylate reductase 3 OS=Danio rerio... [more]
P5CR3_XENLA1.438e-1845.98Pyrroline-5-carboxylate reductase 3 OS=Xenopus lae... [more]
P5CR1_BACSU2.426e-1837.78Pyrroline-5-carboxylate reductase 1 OS=Bacillus su... [more]
P5CR3_MACFA5.346e-1843.82Pyrroline-5-carboxylate reductase 3 OS=Macaca fasc... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06E05_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06E05, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF834867 ID=CF834867; Name=CF834867; organism=Citrus sinensis; type=EST; length=594bp
ATGCAACTGCCCCTTTCAATATCCATACAGAAAACAAAAAAAAGGAGAGG
GTTAATCGCCATACTAGAAGTGGAAGAGCTGAGAAACCGGAATCAAAATG
AATTAGCTCTAGGACAGTTCTCGGCTTCGCGTGAGCAGCAGCAACAACAG
CATTCATTAATATCCCACGAAATCCACTTTTCTCCAACTCATGAATACCA
GCAATAGTAGTCCCACCAGGCGATGCAACATCATCTTTTAGCTGACCAGG
ATGCTTCCCACTTTTAGTGACCATTGATGCTGCTCCTAATACTGTCTGAG
AAGCTAGACCAAGTGCAAGTTCTCGCGGTAGCCCTGCAGCCACAGCTGCA
TCAGCCAAAGCTTCAATTGCTAAAAATATATATGCTGGACCGCTGCCAAG
AAGCCACATACCTCAGGCCCGTGATTGCATCAAACAATTTCTCATCCGCT
CGCCATATCTTGCCAACTGATCCGAATAATTTACCGATTAGTTCCCCATC
CTCTTCTGTTGCAGTCCCTCCCAAGCTCATAACTGTTGCAGCCTCACCTA
CAGCAGAAGGTGTATTAGGCATAACTCTAATAAACCGGCTGTGA
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