CF834868

Overview
NameCF834868
Unique NameCF834868
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length779
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR_MYCTU (Pyrroline-5-carboxylate reductase OS=Mycobacterium tuberculosis GN=proC PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 6.726e-15
Identity = 49/111 (44.14%), Postives = 66/111 (59.46%), Query Frame = 3 
Query:  432 GSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTK---------SGKHPG--------QLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSREL 713
            GSGPAY FL +EAL D GV  GL R++A  LA+QT+ G+A+M+ +         +G+  G        +L+  V SPGGTT A + ELE+ GFR  +  AV AA  RS +L
Sbjct:  180 GSGPAYFFLLVEALVDAGVGVGLSRQVATDLAAQTMAGSAAMLLERMEQDQGGANGELMGLRVDLTASRLRAAVTSPGGTTAAALRELERGGFRMAVDAAVQAAKSRSEQL 290
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR_STAHJ (Pyrroline-5-carboxylate reductase OS=Staphylococcus haemolyticus (strain JCSC1435) GN=proC PE=3 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 8.193e-15
Identity = 30/97 (30.93%), Postives = 49/97 (50.52%), Query Frame = 3 
Query:  426 LLGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSRELS 716
            + GSGPA+++   E     G   GL ++         ++G + M+ +S     QL+ ++ S GGTT AG++ L +     I  + + AA  RS ELS
Sbjct:  170 ITGSGPAFLYHVFEQYVKAGTDLGLEKDQVEESIKNLIIGTSKMIERSDLSMEQLRKNITSKGGTTQAGLNALAQHDIEAIFKDCLNAAVHRSVELS 266

HSP 2 Score: 44.2838 bits (103), Expect = 8.193e-15
Identity = 24/92 (26.09%), Postives = 44/92 (47.83%), Query Frame = 2 
Query:  143 AMQIRPLLSRKKLLVSVAAGVKLKDLQEWTG-HSRFIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITGL 415
            A +I+P ++     +S+ AG+ +  +QE     +   R+MPNT + VG + T +S  G    +  E +  L  + G +   DE     +T +
Sbjct:   79 AQRIQPYINENNRFISIMAGLPINYIQEQLQVENPIARIMPNTNAQVGHSVTGISFSGNFGPKSKEEVNDLVNAFGSVIEVDEDHLHQVTAI 170
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR_TREPA (Pyrroline-5-carboxylate reductase OS=Treponema pallidum GN=proC PE=3 SV=2)

HSP 1 Score: 79.337 bits (194), Expect = 2.556e-14
Identity = 43/93 (46.24%), Postives = 57/93 (61.29%), Query Frame = 3 
Query:  426 LLGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRS 704
            + G  PA+    IEALAD GV  GL R+ A  LA+  + G A+++  SG HP QLKD V SP G+TI G+  LE+ G R  +++AV AA   S
Sbjct:  171 IAGCAPAFAAQFIEALADAGVRYGLARDQAYRLAAHMLEGTAALIQHSGVHPAQLKDRVCSPAGSTIRGVLALEEQGLRRAVIHAVRAALSSS 263
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR_PASMU (Pyrroline-5-carboxylate reductase OS=Pasteurella multocida GN=proC PE=3 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 6.650e-14
Identity = 33/95 (34.74%), Postives = 52/95 (54.74%), Query Frame = 3 
Query:  432 GSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTKSGKHP-GQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSREL 713
            GS PAY FL +EA+        +  + A  L  Q+ LGAA MV ++   P   L+++V S GGTT A +  L +   + I+  A+ A  +RS+++
Sbjct:  177 GSSPAYFFLFMEAMQQALCQMNMDEDTARLLVQQSALGAAKMVIENPDLPIATLRENVTSKGGTTAAALQVLNQQQLQHIVQQAMQACVERSQQM 271

HSP 2 Score: 40.817 bits (94), Expect = 6.650e-14
Identity = 27/81 (33.33%), Postives = 41/81 (50.62%), Query Frame = 2 
Query:  173 KKLLVSVAAGVKLKDLQEWTGHSR-FIRVMPNTPSAVGEAATVMSLGGTATEEDGELIGKLFGSVGK-IWRADEKLFDAIT 409
            +KL++S+AAGV +  LQ     ++  +RVMPNTP+ V E  + +    +   E  +    L  +VGK  W   E     IT
Sbjct:   93 QKLVISIAAGVSVTKLQSRLPTAKQIVRVMPNTPALVAEGMSGLFAQNSLKPEYKQFTQDLLNAVGKTCWVTQEADMHTIT 173
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR_STAS1 (Pyrroline-5-carboxylate reductase OS=Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) GN=proC PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 7.009e-13
Identity = 29/97 (29.90%), Postives = 48/97 (49.48%), Query Frame = 3 
Query:  426 LLGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSRELS 716
            + GSGPA+++   E     G   GL +          ++G + M+ +S     QL+ ++ S GGTT AG++ L +     +  + + AA  RS ELS
Sbjct:  170 ITGSGPAFLYHVFEQYVTAGTRLGLEKAQVEESIRNLIIGTSKMIERSELSMEQLRKNITSKGGTTQAGLNALSQHDLESVFEDCLRAAVDRSVELS 266

HSP 2 Score: 39.6614 bits (91), Expect = 7.009e-13
Identity = 34/128 (26.56%), Postives = 56/128 (43.75%), Query Frame = 2 
Query:   44 ESIGVKVLSDNNAXXXXXXXXXXXXXXXXXXXXAMQIRPLLSRKKLLVSVAAGVKLKDLQE-WTGHSRFIRVMPNTPSAVGEAATVMS----LGGTATEEDGELIGKLFGSVGKIWRADEKLFDAITG 412
            E +GV    D+ A                    A +I+P ++ K   +S+ AG+ +  ++E     +   R+MPNT + VG + T +S     G  + +E  ELI   FGS  ++   +     AITG
Sbjct:   46 EKLGVNYSYDDEALLKAADYVFLGTKPYDFESLAERIKPFITDKNKFISIMAGLPISYIREKLEADNPIARIMPNTNAHVGHSVTGISFSSNFGPNSKDEVDELI-NAFGSAIEVPEDNLHQVTAITG 172
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR_STAAW (Pyrroline-5-carboxylate reductase OS=Staphylococcus aureus (strain MW2) GN=proC PE=3 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 9.103e-13
Identity = 31/97 (31.96%), Postives = 48/97 (49.48%), Query Frame = 3 
Query:  426 LLGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSRELS 716
            + GSGPA+++   E     G   GL +E         ++G + M+ +S     QL+ ++ S GGTT AG+  L +     I  + + AA  RS ELS
Sbjct:  170 ITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSDLSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELS 266

HSP 2 Score: 37.7354 bits (86), Expect = 9.103e-13
Identity = 27/95 (28.42%), Postives = 49/95 (51.58%), Query Frame = 2 
Query:  143 AMQIRPLLSRKKLLVSVAAGVKLKDL-QEWTGHSRFIRVMPNTPSAVGEAATVMSLGGT----ATEEDGELIGKLFGSVGKIWRADEKLFDAITG 412
            A +I+P +++    +S+ AG+ +  + Q+    +   R+MPNT + VG + T +S        + +E  +L+ K FGSV ++         AITG
Sbjct:   79 ATRIKPHITKDNCFISIMAGIPIDYIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLV-KAFGSVIEVSEDHLHQVTAITG 172
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR_STAAS (Pyrroline-5-carboxylate reductase OS=Staphylococcus aureus (strain MSSA476) GN=proC PE=3 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 9.103e-13
Identity = 31/97 (31.96%), Postives = 48/97 (49.48%), Query Frame = 3 
Query:  426 LLGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSRELS 716
            + GSGPA+++   E     G   GL +E         ++G + M+ +S     QL+ ++ S GGTT AG+  L +     I  + + AA  RS ELS
Sbjct:  170 ITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSDLSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELS 266

HSP 2 Score: 37.7354 bits (86), Expect = 9.103e-13
Identity = 27/95 (28.42%), Postives = 49/95 (51.58%), Query Frame = 2 
Query:  143 AMQIRPLLSRKKLLVSVAAGVKLKDL-QEWTGHSRFIRVMPNTPSAVGEAATVMSLGGT----ATEEDGELIGKLFGSVGKIWRADEKLFDAITG 412
            A +I+P +++    +S+ AG+ +  + Q+    +   R+MPNT + VG + T +S        + +E  +L+ K FGSV ++         AITG
Sbjct:   79 ATRIKPHITKDNCFISIMAGIPIDYIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLV-KAFGSVIEVSEDHLHQVTAITG 172
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR_STAAR (Pyrroline-5-carboxylate reductase OS=Staphylococcus aureus (strain MRSA252) GN=proC PE=3 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 9.103e-13
Identity = 31/97 (31.96%), Postives = 48/97 (49.48%), Query Frame = 3 
Query:  426 LLGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSRELS 716
            + GSGPA+++   E     G   GL +E         ++G + M+ +S     QL+ ++ S GGTT AG+  L +     I  + + AA  RS ELS
Sbjct:  170 ITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSDLSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELS 266

HSP 2 Score: 37.7354 bits (86), Expect = 9.103e-13
Identity = 27/95 (28.42%), Postives = 49/95 (51.58%), Query Frame = 2 
Query:  143 AMQIRPLLSRKKLLVSVAAGVKLKDL-QEWTGHSRFIRVMPNTPSAVGEAATVMSLGGT----ATEEDGELIGKLFGSVGKIWRADEKLFDAITG 412
            A +I+P +++    +S+ AG+ +  + Q+    +   R+MPNT + VG + T +S        + +E  +L+ K FGSV ++         AITG
Sbjct:   79 ATRIKPHITKDNCFISIMAGIPIDYIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLV-KAFGSVIEVSEDHLHQVTAITG 172
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR_STAAN (Pyrroline-5-carboxylate reductase OS=Staphylococcus aureus (strain N315) GN=proC PE=1 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 9.103e-13
Identity = 31/97 (31.96%), Postives = 48/97 (49.48%), Query Frame = 3 
Query:  426 LLGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSRELS 716
            + GSGPA+++   E     G   GL +E         ++G + M+ +S     QL+ ++ S GGTT AG+  L +     I  + + AA  RS ELS
Sbjct:  170 ITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSDLSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELS 266

HSP 2 Score: 37.7354 bits (86), Expect = 9.103e-13
Identity = 27/95 (28.42%), Postives = 49/95 (51.58%), Query Frame = 2 
Query:  143 AMQIRPLLSRKKLLVSVAAGVKLKDL-QEWTGHSRFIRVMPNTPSAVGEAATVMSLGGT----ATEEDGELIGKLFGSVGKIWRADEKLFDAITG 412
            A +I+P +++    +S+ AG+ +  + Q+    +   R+MPNT + VG + T +S        + +E  +L+ K FGSV ++         AITG
Sbjct:   79 ATRIKPHITKDNCFISIMAGIPIDYIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLV-KAFGSVIEVSEDHLHQVTAITG 172
[Close]
BLAST of CF834868 vs. ExPASy Swiss-Prot
Match: P5CR_STAAM (Pyrroline-5-carboxylate reductase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) GN=proC PE=1 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 9.103e-13
Identity = 31/97 (31.96%), Postives = 48/97 (49.48%), Query Frame = 3 
Query:  426 LLGSGPAYIFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVTKSGKHPGQLKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSRELS 716
            + GSGPA+++   E     G   GL +E         ++G + M+ +S     QL+ ++ S GGTT AG+  L +     I  + + AA  RS ELS
Sbjct:  170 ITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSDLSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELS 266

HSP 2 Score: 37.7354 bits (86), Expect = 9.103e-13
Identity = 27/95 (28.42%), Postives = 49/95 (51.58%), Query Frame = 2 
Query:  143 AMQIRPLLSRKKLLVSVAAGVKLKDL-QEWTGHSRFIRVMPNTPSAVGEAATVMSLGGT----ATEEDGELIGKLFGSVGKIWRADEKLFDAITG 412
            A +I+P +++    +S+ AG+ +  + Q+    +   R+MPNT + VG + T +S        + +E  +L+ K FGSV ++         AITG
Sbjct:   79 ATRIKPHITKDNCFISIMAGIPIDYIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLV-KAFGSVIEVSEDHLHQVTAITG 172
The following BLAST results are available for this feature:
BLAST of CF834868 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 53
Match NameE-valueIdentityDescription
P5CR_MYCTU6.726e-1544.14Pyrroline-5-carboxylate reductase OS=Mycobacterium... [more]
P5CR_STAHJ8.193e-1530.93Pyrroline-5-carboxylate reductase OS=Staphylococcu... [more]
P5CR_TREPA2.556e-1446.24Pyrroline-5-carboxylate reductase OS=Treponema pal... [more]
P5CR_PASMU6.650e-1434.74Pyrroline-5-carboxylate reductase OS=Pasteurella m... [more]
P5CR_STAS17.009e-1329.90Pyrroline-5-carboxylate reductase OS=Staphylococcu... [more]
P5CR_STAAW9.103e-1331.96Pyrroline-5-carboxylate reductase OS=Staphylococcu... [more]
P5CR_STAAS9.103e-1331.96Pyrroline-5-carboxylate reductase OS=Staphylococcu... [more]
P5CR_STAAR9.103e-1331.96Pyrroline-5-carboxylate reductase OS=Staphylococcu... [more]
P5CR_STAAN9.103e-1331.96Pyrroline-5-carboxylate reductase OS=Staphylococcu... [more]
P5CR_STAAM9.103e-1331.96Pyrroline-5-carboxylate reductase OS=Staphylococcu... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06E05_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06E05, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF834868 ID=CF834868; Name=CF834868; organism=Citrus sinensis; type=EST; length=779bp
CTGCACCGCCGCCCTCTCTAATCTCAAACGCCGTGATGCTTTTGAGTCCA
TCGGCGTCAAAGTATTATCCGACAATAACGCCGTTGTTGAATACAGCGAT
GTGGTTGTTTTCTCTGTGAAACCGCAAGTAGTTAAAGATGTGGCCATGCA
GATAAGGCCGCTGTTATCACGGAAGAAGCTTTTGGTTTCTGTTGCTGCAG
GAGTGAAATTGAAAGACCTTCAGGAATGGACTGGTCACAGCCGGTTTATT
AGAGTTATGCCTAATACACCTTCTGCTGTAGGTGAGGCTGCAACAGTTAT
GAGCTTGGGAGGAACTGCAACAGAAGAGGATGGGGAACTAATCGGTAAAT
TATTCGGATCAGTTGGCAAGATATGGCGAGCGGATGAGAAATTGTTTGAT
GCAATCACGGGCCTGAGGTATGTGGCTTCTTGGCAGCGGTCCAGCATATA
TATTTTTAGCAATTGAAGCTTTGGCTGATGGAGGTGTGGCTGCAGGGCTA
CCGCGAGAACTTGCACTTGGTCTAGCTTCTCAGACAGTATTAGGAGCAGC
ATCAATGGTCACTAAAAGTGGGAAGCATCCTGGTCAGCTAAAAGATGATG
TTGCATCGCCTGGTGGGACTACTATTGCTGGTATTCATGAGTTGGAGAAA
AGTGGATTTCGTGGGATATTAATGAATGCTGTTGTTGCTGCTGCTAAGCG
AAGCCGAGAACTGTCCTAGAGCTAATTCATTTTGATTCCAGTTTCTCAGC
TCTTACACTTCTAGTATGGCGATTAAACC
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