CF834984

Overview
NameCF834984
Unique NameCF834984
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length675
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig5829 contig Csv1_Contig5829:711..1384. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA_DESAH (Serine hydroxymethyltransferase OS=Desulfobacterium autotrophicum (strain ATCC 43914 / DSM 3382 / HRM2) GN=glyA PE=3 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 4.257e-33
Identity = 81/196 (41.33%), Postives = 113/196 (57.65%), Query Frame = 2 
Query:   56 LVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGD-SSALAPGGVRIGTPAMTSRGLLEKDFEQIGEFLHRAV 640
            LVAA    +P  +  IVTTTTHK+LRGPR G+I                +  +   KI+  +FP +QGGP  H I A AVALK+A +P+F  Y +QV ANA  L + L  +G  LV+ GT+NHL+L +L   G++G   E       ITVNKNAV  D    +   G+RIGTP +T+RG+ E + + + E + +A+
Sbjct:  206 LVAAGVHPSPVPHADIVTTTTHKTLRGPRGGLIL---------------SSNEIGPKISSQIFPGIQGGPLMHVIAAKAVALKEALSPSFAQYQRQVVANAATLADALMDRGMKLVSNGTDNHLMLLNLTHNGISGKDAENRLGAAGITVNKNAVPNDPRGPMITSGIRIGTPLVTTRGMGETEMDLVAELITQAL 386
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA_CHRSD (Serine hydroxymethyltransferase OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=glyA PE=3 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 4.257e-33
Identity = 85/197 (43.15%), Postives = 105/197 (53.30%), Query Frame = 2 
Query:   17 GPFCSVIWLISVALVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGD-SSALAPGGVRIGTPAMTSRGLLEKD 604
            G F  V       LVAA    +P  + H+VTTTTHK+LRGPR G+I                   + E K   AVFP  QGGP  H I A A+  K+A  P FKAY +QV  NA A+      +GY +V+GGTE+HL L  L   GLTG   +      +ITVNKNAV  D  S     G+RIGTPA+T+RG  E +
Sbjct:  194 GAFLLVDMAHIAGLVAAGVYPSPLPHAHVVTTTTHKTLRGPRGGLILSAENNA------------EIEKKFQSAVFPGSQGGPLMHVIAAKAICFKEAMEPDFKAYQQQVIDNAKAMAGVFIERGYDVVSGGTEDHLFLLSLVKQGLTGKDADAALGRAHITVNKNAVPNDPQSPFVTSGLRIGTPAVTTRGFGESE 378
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA1_BURTA (Serine hydroxymethyltransferase 1 OS=Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) GN=glyA1 PE=3 SV=2)

HSP 1 Score: 141.354 bits (355), Expect = 4.257e-33
Identity = 80/192 (41.67%), Postives = 109/192 (56.77%), Query Frame = 2 
Query:   56 LVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGD-SSALAPGGVRIGTPAMTSRGLLEKDFEQIGEFL 628
            L+AA    NP  +   VTTTTHKSLRGPR G+I  +                ++E +IN A+FP +QGGP  H I A AVA K+A +P FK Y ++V  NA  L   L  +G  +V+G TE+H++L DLR   +TG   E      +ITVNKNA+  D        GVR+G+PAMT+RG   ++ E +G  +
Sbjct:  208 LIAAGVYPNPVPHADFVTTTTHKSLRGPRGGVILMKA---------------EYEKQINSAIFPGIQGGPLMHVIAAKAVAFKEALSPEFKEYQQKVIENARVLAETLVKRGLRIVSGRTESHVMLVDLRAKNITGKAAEAALGNAHITVNKNAIPNDPEKPFVTSGVRLGSPAMTTRGFGPQEAELVGNLI 384
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA_HAES1 (Serine hydroxymethyltransferase OS=Haemophilus somnus (strain 129Pt) GN=glyA PE=3 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 4.652e-33
Identity = 81/192 (42.19%), Postives = 105/192 (54.69%), Query Frame = 2 
Query:   56 LVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGD-SSALAPGGVRIGTPAMTSRGLLEKDFEQIGEFL 628
            L+AA    +P  + H+VTTTTHK+L GPR G+I                   +   K+N AVFP+ QGGP  H I A AV  K+A  P FK Y  QV  NA A+      +GY++V+ GTENHL L DL   GLTG   +      NITVNKNAV  D        G+R+GTPA+T RG  E+D  ++  ++
Sbjct:  207 LIAAGVYPSPMPHAHVVTTTTHKTLAGPRGGLILANGNE-------------ELYKKLNSAVFPAGQGGPLVHVIAAKAVCFKEALEPEFKTYQAQVVKNAKAMVEVFKQRGYNVVSNGTENHLFLVDLVSHGLTGKAADAALSRANITVNKNAVPNDPQKPFVTSGIRVGTPAVTRRGFNEEDVAELAGWM 385

HSP 2 Score: 21.557 bits (44), Expect = 4.652e-33
Identity = 8/17 (47.06%), Postives = 12/17 (70.59%), Query Frame = 1 
Query:    1 VADPLRALLLCDMAHIS 51
            +AD + A L  DMAH++
Sbjct:  189 IADEVGAYLFVDMAHVA 205
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA3_PSEAE (Serine hydroxymethyltransferase 3 OS=Pseudomonas aeruginosa GN=glyA3 PE=3 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 4.652e-33
Identity = 81/192 (42.19%), Postives = 107/192 (55.73%), Query Frame = 2 
Query:   56 LVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGD-SSALAPGGVRIGTPAMTSRGLLEKDFEQIGEFL 628
            LVAA    NP  +  +VTTTTHK+LRGPR G+I  R             A  + E K+N AVFPS QGGP  H I A AV  K+A  P FK Y +QV  NA ++      +G+ +V+GGT+NHL L  L    +TG   +       ITVNKN+V  D  S     G+RIGTPA+T+RG  E +  ++  ++
Sbjct:  207 LVAAGVYPNPVPFADVVTTTTHKTLRGPRGGLILAR-------------ANEEIEKKLNSAVFPSAQGGPLEHVIAAKAVCFKEALQPEFKTYQQQVLKNAQSMAQVFLDRGFDVVSGGTQNHLFLLSLIKQDITGKDADAALGRAFITVNKNSVPNDPRSPFVTSGLRIGTPAVTTRGFKEAECRELAGWI 385

HSP 2 Score: 21.557 bits (44), Expect = 4.652e-33
Identity = 8/17 (47.06%), Postives = 12/17 (70.59%), Query Frame = 1 
Query:    1 VADPLRALLLCDMAHIS 51
            +AD + A L  DMAH++
Sbjct:  189 IADKVGAYLFVDMAHVA 205
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA_XYLFT (Serine hydroxymethyltransferase OS=Xylella fastidiosa (strain Temecula1 / ATCC 700964) GN=glyA PE=3 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 4.652e-33
Identity = 83/192 (43.23%), Postives = 111/192 (57.81%), Query Frame = 2 
Query:   56 LVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGD-SSALAPGGVRIGTPAMTSRGLLEKDFEQIGEFL 628
            LVAA    +P  + H+VT+TTHK+LRGPR G+I  +           E  V     K+   VFP +QGGP  H I A AVA K+A  PAFK Y +QV  NA A+   L  +GY +V+GGTENHL+L D+    ++G   E      +ITVNKNAV  D  S     G+R+GTPA+T+RG  E+D   +  ++
Sbjct:  207 LVAAGVYPSPLPHAHVVTSTTHKTLRGPRGGIIVAQAPQ--------EALV----KKLQSIVFPGIQGGPLMHVIAAKAVAFKEALEPAFKVYQQQVVKNAKAMAGTLMLRGYKIVSGGTENHLMLVDMIGRDVSGKDAEGALGQVHITVNKNAVPDDPRSPFVTSGLRLGTPAVTTRGYQEQDCVDLAHWI 386

HSP 2 Score: 23.0978 bits (48), Expect = 4.652e-33
Identity = 9/17 (52.94%), Postives = 13/17 (76.47%), Query Frame = 1 
Query:    1 VADPLRALLLCDMAHIS 51
            +AD + A LL DMAH++
Sbjct:  189 IADQVGAYLLVDMAHVA 205
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA_XYLF2 (Serine hydroxymethyltransferase OS=Xylella fastidiosa (strain M23) GN=glyA PE=3 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 4.652e-33
Identity = 83/192 (43.23%), Postives = 111/192 (57.81%), Query Frame = 2 
Query:   56 LVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGD-SSALAPGGVRIGTPAMTSRGLLEKDFEQIGEFL 628
            LVAA    +P  + H+VT+TTHK+LRGPR G+I  +           E  V     K+   VFP +QGGP  H I A AVA K+A  PAFK Y +QV  NA A+   L  +GY +V+GGTENHL+L D+    ++G   E      +ITVNKNAV  D  S     G+R+GTPA+T+RG  E+D   +  ++
Sbjct:  207 LVAAGVYPSPLPHAHVVTSTTHKTLRGPRGGIIVAQAPQ--------EALV----KKLQSIVFPGIQGGPLMHVIAAKAVAFKEALEPAFKVYQQQVVKNAKAMAGTLMLRGYKIVSGGTENHLMLVDMIGRDVSGKDAEGALGQVHITVNKNAVPDDPRSPFVTSGLRLGTPAVTTRGYQEQDCVDLAHWI 386

HSP 2 Score: 23.0978 bits (48), Expect = 4.652e-33
Identity = 9/17 (52.94%), Postives = 13/17 (76.47%), Query Frame = 1 
Query:    1 VADPLRALLLCDMAHIS 51
            +AD + A LL DMAH++
Sbjct:  189 IADQVGAYLLVDMAHVA 205
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA_BACCN (Serine hydroxymethyltransferase OS=Bacillus cereus subsp. cytotoxis (strain NVH 391-98) GN=glyA PE=3 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 4.653e-33
Identity = 82/192 (42.71%), Postives = 108/192 (56.25%), Query Frame = 2 
Query:   56 LVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAV-FGDSSALAPGGVRIGTPAMTSRGLLEKDFEQIGEFL 628
            LVAA    NP  + H VTTTTHK+LRGPR GMI                    F  +I+ ++FP +QGGP  H I A AVA  +A    FK YA+ +  NA  L   L  +G +LV+GGT+NHL+L D+R L +TG   E + D   ITVNKN + F  +S     GVRIGT A+TSRG   ++ ++I   +
Sbjct:  204 LVAAGLHPNPVPHAHFVTTTTHKTLRGPRGGMILCEEK---------------FAKQIDKSIFPGIQGGPLMHVIAAKAVAFGEALQDDFKTYAQNIIHNAQRLAEGLQKEGLTLVSGGTDNHLILIDVRNLNITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIGTAAVTSRGFGLEEMDEIASII 380

HSP 2 Score: 22.7126 bits (47), Expect = 4.653e-33
Identity = 9/17 (52.94%), Postives = 13/17 (76.47%), Query Frame = 1 
Query:    1 VADPLRALLLCDMAHIS 51
            +AD + A L+ DMAHI+
Sbjct:  186 IADEVGAYLMVDMAHIA 202
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA_BACC4 (Serine hydroxymethyltransferase OS=Bacillus cereus (strain B4264) GN=glyA PE=3 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 4.653e-33
Identity = 82/192 (42.71%), Postives = 108/192 (56.25%), Query Frame = 2 
Query:   56 LVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAV-FGDSSALAPGGVRIGTPAMTSRGLLEKDFEQIGEFL 628
            LVAA    NP  + H VTTTTHK+LRGPR GMI                    F  +I+ ++FP +QGGP  H I A AVA  +A    FK YA+ +  NA  L   L  +G +LV+GGT+NHL+L D+R L +TG   E + D   ITVNKN + F  +S     GVRIGT A+TSRG   ++ ++I   +
Sbjct:  204 LVAAGLHPNPVPHAHFVTTTTHKTLRGPRGGMILCEE---------------QFAKQIDKSIFPGIQGGPLMHVIAAKAVAFGEALQDDFKTYAQNIINNANRLAEGLQKEGLTLVSGGTDNHLILIDVRNLEITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIGTAAVTSRGFSLEEMDEIASLI 380

HSP 2 Score: 22.7126 bits (47), Expect = 4.653e-33
Identity = 9/17 (52.94%), Postives = 13/17 (76.47%), Query Frame = 1 
Query:    1 VADPLRALLLCDMAHIS 51
            +AD + A L+ DMAHI+
Sbjct:  186 IADEVGAYLMVDMAHIA 202
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA_CLOB8 (Serine hydroxymethyltransferase OS=Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) GN=glyA PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 4.653e-33
Identity = 77/196 (39.29%), Postives = 106/196 (54.08%), Query Frame = 2 
Query:   56 LVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDS-SALAPGGVRIGTPAMTSRGLLEKDFEQIGEFLHRAV 640
            LVAA    +P  Y   VTTTTHK+LRGPR G+I  +                 +   +N  +FP +QGGP  H I A AV  K+A  P+FK Y + V  N   L   L  +G+ +V+GGT+NH+ L DL    +TG + E L D   ITVNKN V  ++ S     G+RIGT A+T+RG ++ D  +I   +  A+
Sbjct:  206 LVAAGVHPSPVPYSDFVTTTTHKTLRGPRGGLILCKEK---------------YAQILNKNIFPGIQGGPLEHIIAAKAVCFKEALDPSFKTYGENVVENCKELAEQLIARGFKIVSGGTDNHVFLVDLNNKDITGKEAEALLDSVGITVNKNTVPNETRSPFVTSGIRIGTAAITTRGFVKDDMAEIAAVISEAI 386

HSP 2 Score: 24.6386 bits (52), Expect = 4.653e-33
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = 1 
Query:    1 VADPLRALLLCDMAHIS 51
            +AD + ALL+ DMAHI+
Sbjct:  188 IADEVGALLMVDMAHIA 204
The following BLAST results are available for this feature:
BLAST of CF834984 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_DESAH4.257e-3341.33Serine hydroxymethyltransferase OS=Desulfobacteriu... [more]
GLYA_CHRSD4.257e-3343.15Serine hydroxymethyltransferase OS=Chromohalobacte... [more]
GLYA1_BURTA4.257e-3341.67Serine hydroxymethyltransferase 1 OS=Burkholderia ... [more]
GLYA_HAES14.652e-3342.19Serine hydroxymethyltransferase OS=Haemophilus som... [more]
GLYA3_PSEAE4.652e-3342.19Serine hydroxymethyltransferase 3 OS=Pseudomonas a... [more]
GLYA_XYLFT4.652e-3343.23Serine hydroxymethyltransferase OS=Xylella fastidi... [more]
GLYA_XYLF24.652e-3343.23Serine hydroxymethyltransferase OS=Xylella fastidi... [more]
GLYA_BACCN4.653e-3342.71Serine hydroxymethyltransferase OS=Bacillus cereus... [more]
GLYA_BACC44.653e-3342.71Serine hydroxymethyltransferase OS=Bacillus cereus... [more]
GLYA_CLOB84.653e-3339.29Serine hydroxymethyltransferase OS=Clostridium bei... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06H15_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06H15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF834984 ID=CF834984; Name=CF834984; organism=Citrus sinensis; type=EST; length=675bp
GTTGCTGATCCCTTAAGGGCCCTTTTGCTCTGTGATATGGCTCATATCAG
TCGCTCTTGTTGCTGCTCAGGAAGCTGCAAACCCCTTTGAGTACTGTCAC
ATAGTCACAACCACTACTCACAAGAGCTTGCGGGGTCCTAGGGCTGGTAT
GATCTTCTACCGGAAGGGTCCTAAACCACCCAAGAAGGGCCAACCAGAGG
GTGCGGTTTATGACTTTGAAGACAAGATCAACTTTGCTGTCTTCCCATCC
CTTCAAGGTGGTCCTCACAACCACCAGATTGGTGCTCTAGCTGTTGCCCT
GAAACAGGCCTCGACCCCTGCATTCAAGGCCTATGCGAAACAAGTCAAGG
CCAATGCAGTTGCACTTGGGAACTACCTGACGGGCAAGGGATACAGCCTT
GTCACTGGTGGAACTGAGAACCACCTTGTTCTCTGGGATCTACGCCCTCT
TGGATTGACTGGCAATAAGGTTGAGAAGCTCTGTGACCTTTGCAACATTA
CTGTGAACAAGAATGCTGTGTTTGGTGACAGCAGTGCCTTGGCCCCTGGA
GGAGTAAGAATCGGTACTCCTGCCATGACTTCCAGAGGTTTGTTGGAGAA
GGACTTCGAGCAAATTGGAGAGTTTCTTCACCGCGCTGTGACCCTCACGT
TGGAAATTCAAAAGGAGTATGGAAA
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