CF834984

Overview
NameCF834984
Unique NameCF834984
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length675
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig5829 contig Csv1_Contig5829:711..1384. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA_RICAH (Serine hydroxymethyltransferase OS=Rickettsia akari (strain Hartford) GN=glyA PE=3 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 5.741e-38
Identity = 83/192 (43.23%), Postives = 114/192 (59.38%), Query Frame = 2 
Query:   56 LVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAV-FGDSSALAPGGVRIGTPAMTSRGLLEKDFEQIGEFL 628
            LVA  E  +P  Y H+VT+TTHK+LRGPR G++                   +   KIN A+FP LQGGP  H + A AVA  ++  P +K+Y KQ+ +NA AL + L  +GY ++TGGT+NH+VL DLR  G+TG       D   IT NKNA+ F ++S     G+R+GTPA T+RG  E+DF  +G  +
Sbjct:  208 LVATGEHQSPISYAHVVTSTTHKTLRGPRGGLVLSDDE--------------EIGKKINSALFPGLQGGPLMHIVAAKAVAFLESLQPEYKSYIKQIISNAKALASSLQERGYDILTGGTDNHIVLVDLRKNGITGKLAANSLDNAGITCNKNAIPFDETSPFITSGIRLGTPACTTRGFKEQDFVSVGHMV 385
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA_RHOPB (Serine hydroxymethyltransferase OS=Rhodopseudomonas palustris (strain BisB18) GN=glyA PE=3 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 5.741e-38
Identity = 93/207 (44.93%), Postives = 112/207 (54.11%), Query Frame = 2 
Query:   56 LVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAV-FGDSSALAPGGVRIGTPAMTSRGLLEKDFEQIGEFLHRAVTLTLEIQKEYG 673
            LVA    ANP  + H+VTTTTHKSLRGPR G+I                       K N A+FP LQGGP  H I A AVA K+A  P FK YAK V  NA AL   L G G+ +V+GGT+NHL+L DLRP  L GN  EK      IT NKN + +         G+R+GTPA T+RG    +F+Q+G  +  A  L    Q E G
Sbjct:  226 LVAGGVHANPVPHAHVVTTTTHKSLRGPRGGLILTNDEA--------------LAKKFNSAIFPGLQGGPLMHVIAAKAVAFKEALQPDFKVYAKNVVENAKALAETLRGHGFDIVSGGTDNHLMLVDLRPKSLKGNVSEKALVRAGITCNKNGIPYDPEKPFVTSGIRLGTPAATTRGFGVAEFQQVGGMI--AEVLNAIAQSEDG 416
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA_LYSSC (Serine hydroxymethyltransferase OS=Lysinibacillus sphaericus (strain C3-41) GN=glyA PE=3 SV=1)

HSP 1 Score: 157.532 bits (397), Expect = 5.741e-38
Identity = 85/192 (44.27%), Postives = 113/192 (58.85%), Query Frame = 2 
Query:   56 LVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAV-FGDSSALAPGGVRIGTPAMTSRGLLEKDFEQIGEFL 628
            LVA  E  +P  Y   VT+TTHK+LRGPR G+I                A  ++E K+N +VFP +QGGP  H I A AVA  +   P FK YAKQ+K NA AL   L  +G  +V+GGT+NHL+L +++ LGLTG   E   D   IT NKN + +   S     G+RIGTPA+TSRG  E+D +++G  +
Sbjct:  206 LVAVGEHQSPVPYADFVTSTTHKTLRGPRGGLIL---------------ASKEWEQKLNKSVFPGIQGGPLMHVIAAKAVAFGEVLQPEFKDYAKQIKLNAKALAEVLIAEGVEIVSGGTDNHLLLLNVKSLGLTGKVAEHALDEVGITTNKNTIPYDTESPFVTSGIRIGTPAVTSRGFKEEDMKEVGAII 382
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA_CHLCH (Serine hydroxymethyltransferase OS=Chlorobium chlorochromatii (strain CaD3) GN=glyA PE=3 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 7.011e-38
Identity = 87/201 (43.28%), Postives = 114/201 (56.72%), Query Frame = 2 
Query:   56 LVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIF----YRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAV-FGDSSALAPGGVRIGTPAMTSRGLLEKDFEQIGEFLHRAVT 643
            L+AA    NP ++CH VTTTTHK+LRGPR GMI     +               V    + ++  V P +QGGP  H I   AVA  +A  PAFK YA QV  NA  + +     GY++V+GGT+NHL+L DLR   +TG + E L     ITVNKN V F D S     G+RIGTPAMT+RG+ E +  +I E + + +T
Sbjct:  206 LIAAGLLPNPLQHCHFVTTTTHKTLRGPRGGMIMMGSDFENPLGITIKTKTGSRVKMMSEVMDAEVMPGIQGGPLMHIIAGKAVAFGEALQPAFKEYAAQVMKNASTMASRFMELGYTIVSGGTKNHLMLLDLRNKNVTGKEAENLLHEAGITVNKNMVPFDDKSPFVTSGIRIGTPAMTTRGMKEAESRRIAELIDQVIT 406

HSP 2 Score: 21.557 bits (44), Expect = 7.011e-38
Identity = 8/15 (53.33%), Postives = 12/15 (80.00%), Query Frame = 1 
Query:    1 VADPLRALLLCDMAH 45
            +AD + ALL+ D+AH
Sbjct:  188 IADKVGALLMADIAH 202
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA_GLOVI (Serine hydroxymethyltransferase OS=Gloeobacter violaceus GN=gylA PE=3 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 7.014e-38
Identity = 92/197 (46.70%), Postives = 116/197 (58.88%), Query Frame = 2 
Query:   56 LVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAV-FGDSSALAPGGVRIGTPAMTSRGLLEKDFEQIGEFLHRAVT 643
            LV A    NP  +C +VTTTTHK+LRGPR G+I  R           + A+    DK   AVFP  QGGP  H I A AVA  +A  P FK YA  V ANA AL   LT +G +LV+GGT+NHL+L DLR + LTG + + L    NIT NKN + F   S     G+R+G+PAMT+RGL   +F +IGE +   +T
Sbjct:  208 LVVAGVHPNPIPHCDVVTTTTHKTLRGPRGGLILTR-----------DEALGKRFDK---AVFPGTQGGPLEHVIAAKAVAFGEALQPEFKTYAADVVANARALAERLTARGLTLVSGGTDNHLMLVDLRSVDLTGKQADLLMSDVNITTNKNTIPFDPQSPFVTSGLRLGSPAMTTRGLGTTEFGEIGEIIANRLT 390

HSP 2 Score: 22.3274 bits (46), Expect = 7.014e-38
Identity = 9/17 (52.94%), Postives = 13/17 (76.47%), Query Frame = 1 
Query:    1 VADPLRALLLCDMAHIS 51
            +AD + A LL D+AHI+
Sbjct:  190 IADEVGAYLLADIAHIA 206
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA_CHLSY (Serine hydroxymethyltransferase OS=Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl) GN=glyA PE=3 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 7.015e-38
Identity = 85/192 (44.27%), Postives = 115/192 (59.90%), Query Frame = 2 
Query:   56 LVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGD-SSALAPGGVRIGTPAMTSRGLLEKDFEQIGEFL 628
            LVAA E  +P  + H++TTTTHK+LRGPR G+I                   DF  ++N +VFP  QGGP  H I   AVA  +A  P F+ YA Q++ NA AL   L  +G +LV+GGT+NHL+L DLR  GLTG + ++  D   ITVNKNA+  D    +   G+RIGTPA+T+RG+ E +  QI  ++
Sbjct:  205 LVAAGEHPSPVGHAHVITTTTHKTLRGPRGGLILMGD---------------DFAKQLNSSVFPGTQGGPLMHVIAGKAVAFGEALRPEFRQYAAQIRRNARALAEGLMAQGLTLVSGGTDNHLMLVDLRSTGLTGAQAQRALDKAAITVNKNAIPDDPQPPMKTSGIRIGTPAVTTRGMREPEMAQIAAWI 381

HSP 2 Score: 23.483 bits (49), Expect = 7.015e-38
Identity = 9/17 (52.94%), Postives = 14/17 (82.35%), Query Frame = 1 
Query:    1 VADPLRALLLCDMAHIS 51
            +AD + ALL+ D+AHI+
Sbjct:  187 IADEVGALLMADIAHIA 203
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA_CHLAA (Serine hydroxymethyltransferase OS=Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) GN=glyA PE=3 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 7.015e-38
Identity = 85/192 (44.27%), Postives = 115/192 (59.90%), Query Frame = 2 
Query:   56 LVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGD-SSALAPGGVRIGTPAMTSRGLLEKDFEQIGEFL 628
            LVAA E  +P  + H++TTTTHK+LRGPR G+I                   DF  ++N +VFP  QGGP  H I   AVA  +A  P F+ YA Q++ NA AL   L  +G +LV+GGT+NHL+L DLR  GLTG + ++  D   ITVNKNA+  D    +   G+RIGTPA+T+RG+ E +  QI  ++
Sbjct:  205 LVAAGEHPSPVGHAHVITTTTHKTLRGPRGGLILMGD---------------DFAKQLNSSVFPGTQGGPLMHVIAGKAVAFGEALRPEFRQYAAQIRRNARALAEGLMAQGLTLVSGGTDNHLMLVDLRSTGLTGAQAQRALDKAAITVNKNAIPDDPQPPMKTSGIRIGTPAVTTRGMREPEMAQIAAWI 381

HSP 2 Score: 23.483 bits (49), Expect = 7.015e-38
Identity = 9/17 (52.94%), Postives = 14/17 (82.35%), Query Frame = 1 
Query:    1 VADPLRALLLCDMAHIS 51
            +AD + ALL+ D+AHI+
Sbjct:  187 IADEVGALLMADIAHIA 203
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA_OLICO (Serine hydroxymethyltransferase OS=Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) GN=glyA PE=3 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 7.498e-38
Identity = 87/192 (45.31%), Postives = 109/192 (56.77%), Query Frame = 2 
Query:   56 LVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAV-FGDSSALAPGGVRIGTPAMTSRGLLEKDFEQIGEFL 628
            LVA    A+P  + H+ TTTTHKSLRGPR G+I                   D   KIN A+FP LQGGP  H I A AVA K+A  P FK YAK +  NA AL   L G G+ +V+GGT+NHL+L DLRP GL GN  E+      +T NKN + F         G+R+GTPA T+RG    +F+Q+G  +
Sbjct:  219 LVAGGVHASPVPHAHVTTTTTHKSLRGPRGGLILTNDE--------------DIAKKINSAIFPGLQGGPLMHVIAAKAVAFKEALQPDFKVYAKNIVENARALAETLRGHGFEIVSGGTDNHLMLVDLRPKGLKGNISERALVRSGLTCNKNGIPFDPEKPFVTSGLRLGTPATTTRGFGVAEFKQVGALI 396
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA_CYAA5 (Serine hydroxymethyltransferase OS=Cyanothece sp. (strain ATCC 51142) GN=glyA PE=3 SV=1)

HSP 1 Score: 156.762 bits (395), Expect = 9.137e-38
Identity = 87/192 (45.31%), Postives = 113/192 (58.85%), Query Frame = 2 
Query:   56 LVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAV-FGDSSALAPGGVRIGTPAMTSRGLLEKDFEQIGEFL 628
            LVA+    NP  YC +VTTTTHK+LRGPR G+I                   +   + + AVFP  QGGP    I A AVA  +A  P FK Y+ QV ANA AL N L  +G+ LV+GGT+NHL+L DLR + +TG + +KL    NIT NKN V F   S     G+R+G+PAMT+RGL  ++F +IG  +
Sbjct:  209 LVASGHHPNPLPYCDVVTTTTHKTLRGPRGGLIMTNNP--------------ELGKQFDKAVFPGTQGGPLEQVIAAKAVAFGEALKPEFKVYSGQVIANAQALANQLNQRGFKLVSGGTDNHLMLVDLRCIDMTGKEADKLVSEINITANKNTVPFDPESPFVTSGLRLGSPAMTTRGLGVEEFREIGNII 386

HSP 2 Score: 21.557 bits (44), Expect = 9.137e-38
Identity = 8/17 (47.06%), Postives = 13/17 (76.47%), Query Frame = 1 
Query:    1 VADPLRALLLCDMAHIS 51
            +AD + A L+ D+AHI+
Sbjct:  191 IADEVGAYLMADIAHIA 207
[Close]
BLAST of CF834984 vs. ExPASy Swiss-Prot
Match: GLYA_PELPB (Serine hydroxymethyltransferase OS=Pelodictyon phaeoclathratiforme (strain DSM 5477 / BU-1) GN=glyA PE=3 SV=1)

HSP 1 Score: 156.762 bits (395), Expect = 9.792e-38
Identity = 88/201 (43.78%), Postives = 111/201 (55.22%), Query Frame = 2 
Query:   56 LVAAQEAANPFEYCHIVTTTTHKSLRGPRAGMIF----YRXXXXXXXXXXXEGAVYDFEDKINFAVFPSLQGGPHNHQIGALAVALKQASTPAFKAYAKQVKANAVALGNYLTGKGYSLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAV-FGDSSALAPGGVRIGTPAMTSRGLLEKDFEQIGEFLHRAVT 643
            L+AA    NP  +CH VTTTTHK+LRGPR GMI     +               +    + ++  V P +QGGP  H I   AVA  +A  PAF+ YA QV  NA A+    T  GY +V+GGT+NHL+L DLR   +TG   E L     ITVNKN V F D S     G+R+GTPAMT+RG+ E D   I E + R +T
Sbjct:  206 LIAAGLLGNPLPHCHFVTTTTHKTLRGPRGGMIMMGSDFENPMGITIKTKTGSRLKMMSEVMDAEVMPGIQGGPLMHIIAGKAVAFGEALQPAFRDYAAQVIKNAAAMAEKFTELGYKIVSGGTKNHLMLLDLRSKNVTGKVAENLLHSAGITVNKNMVPFDDKSPFVTSGIRVGTPAMTTRGMNEADSVLIAELIDRVIT 406
The following BLAST results are available for this feature:
BLAST of CF834984 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_RICAH5.741e-3843.23Serine hydroxymethyltransferase OS=Rickettsia akar... [more]
GLYA_RHOPB5.741e-3844.93Serine hydroxymethyltransferase OS=Rhodopseudomona... [more]
GLYA_LYSSC5.741e-3844.27Serine hydroxymethyltransferase OS=Lysinibacillus ... [more]
GLYA_CHLCH7.011e-3843.28Serine hydroxymethyltransferase OS=Chlorobium chlo... [more]
GLYA_GLOVI7.014e-3846.70Serine hydroxymethyltransferase OS=Gloeobacter vio... [more]
GLYA_CHLSY7.015e-3844.27Serine hydroxymethyltransferase OS=Chloroflexus au... [more]
GLYA_CHLAA7.015e-3844.27Serine hydroxymethyltransferase OS=Chloroflexus au... [more]
GLYA_OLICO7.498e-3845.31Serine hydroxymethyltransferase OS=Oligotropha car... [more]
GLYA_CYAA59.137e-3845.31Serine hydroxymethyltransferase OS=Cyanothece sp. ... [more]
GLYA_PELPB9.792e-3843.78Serine hydroxymethyltransferase OS=Pelodictyon pha... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06H15_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06H15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF834984 ID=CF834984; Name=CF834984; organism=Citrus sinensis; type=EST; length=675bp
GTTGCTGATCCCTTAAGGGCCCTTTTGCTCTGTGATATGGCTCATATCAG
TCGCTCTTGTTGCTGCTCAGGAAGCTGCAAACCCCTTTGAGTACTGTCAC
ATAGTCACAACCACTACTCACAAGAGCTTGCGGGGTCCTAGGGCTGGTAT
GATCTTCTACCGGAAGGGTCCTAAACCACCCAAGAAGGGCCAACCAGAGG
GTGCGGTTTATGACTTTGAAGACAAGATCAACTTTGCTGTCTTCCCATCC
CTTCAAGGTGGTCCTCACAACCACCAGATTGGTGCTCTAGCTGTTGCCCT
GAAACAGGCCTCGACCCCTGCATTCAAGGCCTATGCGAAACAAGTCAAGG
CCAATGCAGTTGCACTTGGGAACTACCTGACGGGCAAGGGATACAGCCTT
GTCACTGGTGGAACTGAGAACCACCTTGTTCTCTGGGATCTACGCCCTCT
TGGATTGACTGGCAATAAGGTTGAGAAGCTCTGTGACCTTTGCAACATTA
CTGTGAACAAGAATGCTGTGTTTGGTGACAGCAGTGCCTTGGCCCCTGGA
GGAGTAAGAATCGGTACTCCTGCCATGACTTCCAGAGGTTTGTTGGAGAA
GGACTTCGAGCAAATTGGAGAGTTTCTTCACCGCGCTGTGACCCTCACGT
TGGAAATTCAAAAGGAGTATGGAAA
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