CF835073

Overview
NameCF835073
Unique NameCF835073
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length706
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDI_HORVU (Protein disulfide-isomerase OS=Hordeum vulgare GN=PDI PE=1 SV=2)

HSP 1 Score: 90.5077 bits (223), Expect = 9.395e-18
Identity = 53/161 (32.92%), Postives = 87/161 (54.04%), Query Frame = -3
Query:  195 IADEDLAKPFLTLFGLEESKNTVVTAFDNKAISKFLLESDLTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHP-KLQVEEYPTLLFY-PAGDKAN 671
            I D + ++     FGL+  +  ++   D    SK  L+  +    I  +      G LTP+ KS+PIP+  N  V++VV     D+V  S K+VL+E Y PWC  C+  +  +++ A   +  +++VIAK+DA+ N+ P +  V+ YPTL F  P+G K +
Sbjct:  311 IGDIESSQGAFQYFGLKVDQAPLILIQDGD--SKKFLKEHVEAGQIVAWLKDYFDGKLTPFRKSEPIPEANNEPVKVVVADNVHDVVFKSGKNVLIEFYAPWCGHCKKLAPILDEAAATLQSEEDVVIAKMDATENDVPGEFDVQGYPTLYFVTPSGKKVS 469          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: EUG1_YEAST (Protein disulfide-isomerase EUG1 OS=Saccharomyces cerevisiae GN=EUG1 PE=1 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 3.570e-17
Identity = 52/161 (32.30%), Postives = 83/161 (51.55%), Query Frame = -3
Query:   63 GTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKG----LDNLVIAKIDASANEHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQ---------LKEKDQSPKDEQLKERDQAPKDEL 506
            GT  P +KS+ IP    +NV  +VGKT DD+V +  KDVL++ Y  WC+  +  +   E++A          D ++IA++D+ AN+     V  YPT+  YPAG+ + PI  +   + +++  FIKE          + +K    KD ++   D    DEL
Sbjct:  358 GTAKPIVKSEEIPKEQKSNVYKIVGKTHDDIVHDDDKDVLVKYYATWCIHSKRFAPIYEEIANVLASDESVRDKILIAEVDSGANDILSFPVTGYPTIALYPAGNNSKPIIFNKIRNLEDVFEFIKESGTHHIDGQAIYDKLHQAKDSEVSTEDTV-HDEL 517          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDI1_CAEEL (Protein disulfide-isomerase 1 OS=Caenorhabditis elegans GN=pdi-1 PE=3 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 2.314e-16
Identity = 55/204 (26.96%), Postives = 101/204 (49.51%), Query Frame = -3
Query:   99 RERFMFTAVDIADEDLAK--PFLTLFGLEESKNTVVTAFDNKAISKFLLESDLTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNA-NVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLKEKDQSPKDEQ 701
            R + +F  +D+  E+ A+   FL +       N +V+  D   + KF  +      + E F +  L G     LK+Q +P++ NA  V+++V   F+++ L+  K V ++ Y PWC  C+      ++LA+ ++   N+VIAK+DA+ NE   ++V  +PTL  +PAG  + P+      + +    F+ +      +S    Q
Sbjct:  283 RAKIVFVLLDVDVEENARILEFLGVDAKNTPANRIVSLADQ--VEKFKPQEG---EDFEAFTNSYLEGKSAQDLKAQDLPEDWNALPVKVLVASNFNEIALDETKTVFVKFYAPWCGHCKQLVPVWDELAEKYESNPNVVIAKLDATLNELADVKVNSFPTLKLWPAG-SSTPVDYDGDRNLEKFEEFVNKYAGSASESETASQ 480          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDI_ALTAL (Protein disulfide-isomerase (Fragment) OS=Alternaria alternata PE=1 SV=2)

HSP 1 Score: 78.5666 bits (192), Expect = 3.694e-14
Identity = 42/117 (35.90%), Postives = 68/117 (58.12%), Query Frame = -3
Query:  219 ESDLTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKG--LDNLV-IAKIDASANEHPKLQVEEYPTLLF 560
            E+ +T   I +F    L G + P +KS+PIP++ +  V +VV   + D+V+++ KDVL+E Y PWC  C+  + + E+L + +    L  LV IAK+DA+ N+ P       P+ LF
Sbjct:  201 EAKITEKEIGKFVDDFLAGKIDPSIKSEPIPESNDGPVTVVVAHNYKDVVIDNDKDVLVEFYAPWCGHCKALAPKYEELGQLYASDELSKLVTIAKVDATLNDVPDEIQGFLPSSLF 317          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDILT_HUMAN (Protein disulfide-isomerase-like protein of the testis OS=Homo sapiens GN=PDILT PE=1 SV=2)

HSP 1 Score: 76.6406 bits (187), Expect = 1.404e-13
Identity = 50/215 (23.26%), Postives = 105/215 (48.84%), Query Frame = -3
Query:   63 RERFMFTAVDIADEDLAKPFLTLFGLEESKNTVVTAFDNKAISKFLLESD-LTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNAN-VQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLKEKDQSPKDEQLKERDQAPKDEL 701
            + + +F  VD ADE         F + E     V   +  + +++ + SD +T  ++++F    L    T +  S+ IP   +   V+ +VGK F+ +V +  KDV +  Y PW  +C+     +E+L + ++    ++IAKID +AN+   + ++ YP    +P+G +   +      + K  + F++  +K K +   +    E+++  ++E+
Sbjct:  304 QNKILFILVD-ADEPRNGRVFKYFRVTEVDIPSVQILNLSSDARYKMPSDDITYESLKKFGRSFLSKNATKHQSSEEIPKYWDQGLVKQLVGKNFNVVVFDKEKDVFVMFYAPWSKKCKMLFPLLEELGRKYQNHSTIIIAKIDVTANDIQLMYLDRYPFFRLFPSGSQ-QAVLYKGEHTLKGFSDFLESHIKTKIEDEDELLSVEQNEVIEEEV 516          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDILT_RAT (Protein disulfide-isomerase-like protein of the testis OS=Rattus norvegicus GN=Pdilt PE=1 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.833e-13
Identity = 52/230 (22.61%), Postives = 108/230 (46.96%), Query Frame = -3
Query:   18 RERFMFTAVDIADEDLAKPFLTLFGLEESKNTVVTAFDNKAISKFLLESD-LTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNAN-VQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLKEKDQSPKDEQLKERDQAPKDEL*IL*KGWNIETLTEK 701
            + + +F  V+ +DE   K     F +       V   +  + +++ + +D +T  ++++FC+  L  T   +  S+ IP   +   V+ +VGK F+ +V +  KDV +  Y PW  +C      +E+L   ++    ++IAKID +AN+      E+YP    +P  D    +      + K    F++  +K + +   +    E+++  ++E+    +  +I+ L EK
Sbjct:  301 QNKILFVLVN-SDEPKNKRIFEYFQISRVNVPSVQILNLSSDARYKMPTDNITFESLKKFCNSFLSRTAKKHKSSEEIPKYWDQEPVKKLVGKNFNVVVFDKEKDVFVMFYAPWSEKCRVLLPLLEELGIKYQNHSTVIIAKIDITANDIQLANPEQYPFFRLFPT-DSQEAVMYKGEHTMKGFCDFLESHVKVRIEEDDELLYIEQNEVAEEEVLAEPEMQHIDKLPEK 528          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: BS2_TRYBB (Bloodstream-specific protein 2 OS=Trypanosoma brucei brucei GN=BS2 PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.833e-13
Identity = 47/164 (28.66%), Postives = 82/164 (50.00%), Query Frame = -3
Query:   69 LTPSNIEEFCSRLLHGTLTPYLKSQPIPD-NTNANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASAN--EHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLKEKDQSPKDEQLKERDQAPKD 551
            +T  ++E+F      G + P +KS P+P+  T      +V KT     L S KD+L+  + PWC  C+  +   +K+AK F   D L++A++DA+AN        V  +PT+ F P G K  P+      S +N+  F+++ +     S K   + E  ++ ++
Sbjct:  316 VTRESLEKFILEFAAGRVEPTIKSLPVPEVETVDGKTTIVAKTMQKH-LTSGKDMLILFFAPWCGHCKNFAPTFDKIAKEFDATD-LIVAELDATANYVNSSTFTVTAFPTVFFVPNGGK--PVVFEGERSFENVYEFVRKHVTTFKVSEKPANVTEEKKSEEE 475          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDILT_MOUSE (Protein disulfide-isomerase-like protein of the testis OS=Mus musculus GN=Pdilt PE=1 SV=2)

HSP 1 Score: 75.8702 bits (185), Expect = 2.395e-13
Identity = 54/224 (24.11%), Postives = 105/224 (46.88%), Query Frame = -3
Query:   48 RERFMFTAVDIADEDLAKPFLTLFGLEESKNTVVTAFDNKAISKFLLESD-LTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNAN-VQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAF----IKEQLKEKDQSPKDEQLKERDQAPKDEL*IL*K 701
            + + +F  V+ ADE   K     F +       V   +  +  ++ + +D +   ++++FC+  L  T   +  S+ IP   +   V+ +VGK F+ +VL+  KDV +  Y PW  +C      +E+L   ++    ++IAKID +AN+      E+YP    +P  D    +      + K    F    +K +++E+D+    EQ +E +   + E+ ++ K
Sbjct:  301 QNKILFVLVN-ADEPKNKRIFEYFQISRVNVPSVQILNLSSDGRYKMPTDDINFESLKKFCNSFLSKTAKKHKASEEIPKYWDQGPVKKLVGKNFNVVVLDKEKDVFVMFYAPWSEKCRVLLPLLEELGIKYQNHSTVIIAKIDITANDIQLANPEQYPFFRLFPT-DSQEAVMYKGEHTMKGFCDFLESHVKVRIEEEDELLYIEQNEEEEVLAEPEIQLIEK 522          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDIA6_MEDSA (Probable protein disulfide-isomerase A6 OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 9.099e-13
Identity = 41/109 (37.61%), Postives = 63/109 (57.80%), Query Frame = -3
Query:  135 ANVQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEH--PKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQ 455
            ++V ++  +TF+++VL+  KDVL+E Y PWC  C++ +   EK+A  FK  D++VIA +DA        K  V  +PTL F+P G+KA       R    +  AFI E+
Sbjct:  147 SHVVVLTPETFNEVVLDGTKDVLVEFYAPWCGHCKSLAPIYEKVAAVFKSEDDVVIANLDADKYRDLAEKYDVSGFPTLKFFPKGNKAGEDYGGGRDLD-DFVAFINEK 254          
BLAST of CF835073 vs. ExPASy Swiss-Prot
Match: PDILT_MACFA (Protein disulfide-isomerase-like protein of the testis OS=Macaca fascicularis GN=PDILT PE=2 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 1.188e-12
Identity = 48/215 (22.33%), Postives = 104/215 (48.37%), Query Frame = -3
Query:   63 RERFMFTAVDIADEDLAKPFLTLFGLEESKNTVVTAFDNKAISKFLLESD-LTPSNIEEFCSRLLHGTLTPYLKSQPIPDNTNAN-VQIVVGKTFDDLVLNSHKDVLLEVYTPWCVRCETTSKQIEKLAKHFKGLDNLVIAKIDASANEHPKLQVEEYPTLLFYPAGDKANPIKVSARSSSKNIAAFIKEQLKEKDQSPKDEQLKERDQAPKDEL 701
            + + +F  V+ ADE   +  +  F + E     V   +  + +++ + SD +T  N+++F    L      +  S+ IP + +   V+ +VGK F+ +V +  KDV +  Y PW  +C+     +E+L + ++    ++IAKID +AN+   + ++ YP    +P  D    +      + K  + F++  +K   +   +    E+++  ++E+
Sbjct:  304 QNKILFILVN-ADEPRNRRVIEYFRVTEVDIPSVQILNLSSDARYKMPSDDITYENLKKFGRSFLSKNAKKHQSSEEIPKHWDQGLVKQLVGKNFNIVVFDKEKDVFVMFYAPWSKKCKMLFPLLEELGRKYQNHSTIIIAKIDITANDIQLVYLDRYPFFRLFPT-DSQQAVLYKGEHTLKGFSDFLESYIKTSIEDEDELLSVEQNEVIEEEV 516          
The following BLAST results are available for this feature:
BLAST of CF835073 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 51
Match NameE-valueIdentityDescription
PDI_HORVU9.395e-1832.92Protein disulfide-isomerase OS=Hordeum vulgare GN=... [more]
EUG1_YEAST3.570e-1732.30Protein disulfide-isomerase EUG1 OS=Saccharomyces ... [more]
PDI1_CAEEL2.314e-1626.96Protein disulfide-isomerase 1 OS=Caenorhabditis el... [more]
PDI_ALTAL3.694e-1435.90Protein disulfide-isomerase (Fragment) OS=Alternar... [more]
PDILT_HUMAN1.404e-1323.26Protein disulfide-isomerase-like protein of the te... [more]
PDILT_RAT1.833e-1322.61Protein disulfide-isomerase-like protein of the te... [more]
BS2_TRYBB1.833e-1328.66Bloodstream-specific protein 2 OS=Trypanosoma bruc... [more]
PDILT_MOUSE2.395e-1324.11Protein disulfide-isomerase-like protein of the te... [more]
PDIA6_MEDSA9.099e-1337.61Probable protein disulfide-isomerase A6 OS=Medicag... [more]
PDILT_MACFA1.188e-1222.33Protein disulfide-isomerase-like protein of the te... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06J24_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06J24, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835073 ID=CF835073; Name=CF835073; organism=Citrus sinensis; type=EST; length=706bp
TTTTTTTTTTTTTTTCCTTTTTCTGTTAAAGTTTCAATATTCCACCCCTT
TTATAAGATCTATAATTCATCTTTGGGAGCTTGATCCCTTTCTTTCAATT
GTTCATCTTTTGGGGATTGATCTTTTTCTTTCAATTGTTCCTTGATAAAT
GCTGCTATGTTCTTTGAGCTGGACCTTGCTGAAACTTTGATCGGATTTGC
TTTATCACCAGCTGGGTAGAATAAAAGTGTTGGGTACTCTTCAACCTGCA
GTTTTGGATGCTCATTTGCAGAAGCATCTATCTTTGCAATGACTAGATTG
TCCAAACCCTTGAAGTGCTTAGCCAGCTTTTCGATTTGCTTGCTAGTTGT
CTCACATCTGACACACCATGGTGTATATACCTCTAGAAGAACATCCTTGT
GACTGTTCAAAACCAAGTCGTCAAATGTCTTTCCCACAACAATCTGCACG
TTTGCGTTTGTGTTATCTGGTATTGGCTGTGATTTCAAGTAAGGCGTCAG
AGTACCATGCAAAAGCCTTGAGCAAAACTCTTCTATGTTGCTTGGCGTGA
GATCTGACTCCAACAAAAACTTTGAAATGGCTTTGTTATCAAAGGCAGTC
ACCACAGTGTTCTTTGATTCTTCAAGCCCAAATAAAGTTAAAAATGGCTT
CGCCAGGTCTTCATCTGCGATATCTACTGCTGTAAACATGAATCTTTCCC
TTGAAA
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