CF835163

Overview
NameCF835163
Unique NameCF835163
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length657
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CF835163_ssr113 microsatellite CF835163_ssr113:113..128. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig10947 contig Csv1_Contig10947:93..750. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK1_ELAUM (S-adenosylmethionine synthetase 1 OS=Elaeagnus umbellata GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 251.906 bits (642), Expect = 2.145e-66
Identity = 125/145 (86.21%), Postives = 130/145 (89.66%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVA+GLARRC+VQVSYAIGVPEPLSVFVDSYGTG+IPDKEIL IVKENFDFRPGMI+INLDLKRGGNGRFLKTA YGHFGRDDPDFTWEIVKPLKWEK Q+
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCLVQVSYAIGVPEPLSVFVDSYGTGQIPDKEILNIVKENFDFRPGMITINLDLKRGGNGRFLKTAGYGHFGRDDPDFTWEIVKPLKWEKPQS 393
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK2_SOLLC (S-adenosylmethionine synthetase 2 OS=Solanum lycopersicum GN=SAM2 PE=2 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 2.801e-66
Identity = 125/145 (86.21%), Postives = 130/145 (89.66%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVA+GLARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEIL IVKENFDFRPGMISINLDL RGGNGRFLKTAAYGHFGRDDPDFTWE+VKPLKW+K +A
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILNIVKENFDFRPGMISINLDLLRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWDKPEA 393
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK2_CATRO (S-adenosylmethionine synthetase 2 OS=Catharanthus roseus GN=SAMS2 PE=1 SV=1)

HSP 1 Score: 251.136 bits (640), Expect = 3.658e-66
Identity = 125/145 (86.21%), Postives = 132/145 (91.03%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKENFDFRPGMI+INLDLKRGGN RFLKTAAYGHFGR+DPDFTWE+VKPLK+EK++A
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKENFDFRPGMIAINLDLKRGGNSRFLKTAAYGHFGREDPDFTWEVVKPLKFEKVEA 393
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_SOLBR (S-adenosylmethionine synthetase OS=Solanum brevidens GN=SAMS PE=2 SV=1)

HSP 1 Score: 250.751 bits (639), Expect = 4.778e-66
Identity = 125/144 (86.81%), Postives = 129/144 (89.58%), Query Frame = -3 
Query:  224 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQ 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPD+EILKIVKENFDFRPGM+SINLDLKRGGNGRFLKTAAYGHFGRDD DFTWE+VKPLKWE  Q
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDREILKIVKENFDFRPGMMSINLDLKRGGNGRFLKTAAYGHFGRDDADFTWEVVKPLKWENPQ 392
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_PHALU (S-adenosylmethionine synthetase OS=Phaseolus lunatus GN=SAMS PE=2 SV=1)

HSP 1 Score: 250.366 bits (638), Expect = 6.240e-66
Identity = 125/142 (88.03%), Postives = 128/142 (90.14%), Query Frame = -3 
Query:  230 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEK 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVAS LARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGN RFLKTAAYGHFGR+DPDFTWE+VKPLKWEK
Sbjct:  250 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASELARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNSRFLKTAAYGHFGREDPDFTWEVVKPLKWEK 391
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK3_ACTCH (S-adenosylmethionine synthetase 3 (Fragment) OS=Actinidia chinensis GN=SAM3 PE=2 SV=1)

HSP 1 Score: 249.98 bits (637), Expect = 8.150e-66
Identity = 125/144 (86.81%), Postives = 130/144 (90.28%), Query Frame = -3 
Query:  224 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQ 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE+FDFRPGMI+I+LDLKRGGNGRFLKTAAYGHFGRDD DFTWE+VKPLKWEK Q
Sbjct:  216 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKESFDFRPGMIAIHLDLKRGGNGRFLKTAAYGHFGRDDADFTWEVVKPLKWEKPQ 359
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK1_ORYSJ (S-adenosylmethionine synthetase 1 OS=Oryza sativa subsp. japonica GN=SAM1 PE=2 SV=1)

HSP 1 Score: 249.98 bits (637), Expect = 8.150e-66
Identity = 123/145 (84.83%), Postives = 129/145 (88.97%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAY+ RQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVD+YGTG+IPDKEILKIVKENFDFRPGMI INLDLK+GGNGR+LKTAAYGHFGRDDPDFTWE+VKPLKWEK  A
Sbjct:  252 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYVARQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDTYGTGRIPDKEILKIVKENFDFRPGMIIINLDLKKGGNGRYLKTAAYGHFGRDDPDFTWEVVKPLKWEKPSA 396
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK1_ORYSI (S-adenosylmethionine synthetase 1 OS=Oryza sativa subsp. indica GN=SAM1 PE=2 SV=2)

HSP 1 Score: 249.98 bits (637), Expect = 8.150e-66
Identity = 123/145 (84.83%), Postives = 129/145 (88.97%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAY+ RQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVD+YGTG+IPDKEILKIVKENFDFRPGMI INLDLK+GGNGR+LKTAAYGHFGRDDPDFTWE+VKPLKWEK  A
Sbjct:  252 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYVARQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDTYGTGRIPDKEILKIVKENFDFRPGMIIINLDLKKGGNGRYLKTAAYGHFGRDDPDFTWEVVKPLKWEKPSA 396
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK5_POPTR (S-adenosylmethionine synthetase 5 OS=Populus trichocarpa GN=METK5 PE=2 SV=1)

HSP 1 Score: 249.595 bits (636), Expect = 1.064e-65
Identity = 126/146 (86.30%), Postives = 132/146 (90.41%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWE-KLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEIL+IVKE+FDFRPGMISINLDLKRGGN RFLKTAAYGHFGRDDPDFTWE+VKPLKW+ K+QA
Sbjct:  250 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILQIVKESFDFRPGMISINLDLKRGGNSRFLKTAAYGHFGRDDPDFTWEVVKPLKWDNKVQA 395
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK3_POPTR (S-adenosylmethionine synthetase 3 OS=Populus trichocarpa GN=METK3 PE=2 SV=1)

HSP 1 Score: 249.595 bits (636), Expect = 1.064e-65
Identity = 126/146 (86.30%), Postives = 132/146 (90.41%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWE-KLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEIL+IVKE+FDFRPGMISINLDLKRGGN RFLKTAAYGHFGRDDPDFTWE+VKPLKW+ K+QA
Sbjct:  250 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILQIVKESFDFRPGMISINLDLKRGGNSRFLKTAAYGHFGRDDPDFTWEVVKPLKWDNKVQA 395
The following BLAST results are available for this feature:
BLAST of CF835163 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK1_ELAUM2.145e-6686.21S-adenosylmethionine synthetase 1 OS=Elaeagnus umb... [more]
METK2_SOLLC2.801e-6686.21S-adenosylmethionine synthetase 2 OS=Solanum lycop... [more]
METK2_CATRO3.658e-6686.21S-adenosylmethionine synthetase 2 OS=Catharanthus ... [more]
METK_SOLBR4.778e-6686.81S-adenosylmethionine synthetase OS=Solanum brevide... [more]
METK_PHALU6.240e-6688.03S-adenosylmethionine synthetase OS=Phaseolus lunat... [more]
METK3_ACTCH8.150e-6686.81S-adenosylmethionine synthetase 3 (Fragment) OS=Ac... [more]
METK1_ORYSJ8.150e-6684.83S-adenosylmethionine synthetase 1 OS=Oryza sativa ... [more]
METK1_ORYSI8.150e-6684.83S-adenosylmethionine synthetase 1 OS=Oryza sativa ... [more]
METK5_POPTR1.064e-6586.30S-adenosylmethionine synthetase 5 OS=Populus trich... [more]
METK3_POPTR1.064e-6586.30S-adenosylmethionine synthetase 3 OS=Populus trich... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06M13_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06M13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835163 ID=CF835163; Name=CF835163; organism=Citrus sinensis; type=EST; length=657bp
TTGGTTTACCCTTAAGAGAATTTCAGAACAATTCATATTCGCCTCTCCCC
CCATTATGCCTTGGATTGAGAATCCATTAAACATATTTATTAAAAAAAAA
GGGGAAAAGAAGAAGAAAGAAAGAAAGAAAACATTCATGTAACTGAAACA
GAAGTAAAAAAGAAAAGTAGACATATATAAGCACCAATGAGAGGCCAGCA
GCTCATCCTTGATTAGTTTAAGCTTGAAGTTTCTCCCACTTGAGTGGCTT
AACAATCTCCCAAGTGAAATCAGGATCATCTCTGCCAAAGTGCCCATAAG
CGGCAGTCTTCAAGAACCTGCCGTTGCCACCCCTCTTGAGATCAAGATTA
ATAGAAATCATCCCAGGCCTAAAATCAAAATTCTCCTTCACAATCTTCAA
AATTTCCTTGTCAGGGATCTTCCCAGTCCCATAACTATCAACAAAAACTG
ACAAAGGCTCGGGCACACCAATAGCGTAAGAAACCTGAACAATGCATCTC
CTGGCAAGCCCGCTTGCCACAATACTCTTAGCAGCTTGCCTTACAATGTA
AGCACCGCTTCTATCAACCTTGGTAGGATCTTTCCCTGAGAAAGCCCCAC
CACCGTGTGCCCCCCAGCCACCATAAGTATCAATAATAATCTTCCGGCCG
GTGAGCC
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