CF835163

Overview
NameCF835163
Unique NameCF835163
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length657
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CF835163_ssr113 microsatellite CF835163_ssr113:113..128. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig10947 contig Csv1_Contig10947:93..750. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_ECO7I (S-adenosylmethionine synthetase OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=metK PE=3 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 1.041e-28
Identity = 69/133 (51.88%), Postives = 89/133 (66.92%), Query Frame = -3 
Query:  257 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 655
            LTGRKII+DTY            GKDP+KVDRS AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  242 LTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_ECO5E (S-adenosylmethionine synthetase OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=metK PE=3 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 1.041e-28
Identity = 69/133 (51.88%), Postives = 89/133 (66.92%), Query Frame = -3 
Query:  257 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 655
            LTGRKII+DTY            GKDP+KVDRS AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  242 LTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_ECO57 (S-adenosylmethionine synthetase OS=Escherichia coli O157:H7 GN=metK PE=3 SV=2)

HSP 1 Score: 126.716 bits (317), Expect = 1.041e-28
Identity = 69/133 (51.88%), Postives = 89/133 (66.92%), Query Frame = -3 
Query:  257 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 655
            LTGRKII+DTY            GKDP+KVDRS AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  242 LTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_ECO55 (S-adenosylmethionine synthetase OS=Escherichia coli (strain 55989 / EAEC) GN=metK PE=3 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 1.041e-28
Identity = 69/133 (51.88%), Postives = 89/133 (66.92%), Query Frame = -3 
Query:  257 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 655
            LTGRKII+DTY            GKDP+KVDRS AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  242 LTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_ECO27 (S-adenosylmethionine synthetase OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=metK PE=3 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 1.041e-28
Identity = 69/133 (51.88%), Postives = 89/133 (66.92%), Query Frame = -3 
Query:  257 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 655
            LTGRKII+DTY            GKDP+KVDRS AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  242 LTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_ECO24 (S-adenosylmethionine synthetase OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=metK PE=3 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 1.041e-28
Identity = 69/133 (51.88%), Postives = 89/133 (66.92%), Query Frame = -3 
Query:  257 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 655
            LTGRKII+DTY            GKDP+KVDRS AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  242 LTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_CITK8 (S-adenosylmethionine synthetase OS=Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) GN=metK PE=3 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 1.041e-28
Identity = 69/133 (51.88%), Postives = 89/133 (66.92%), Query Frame = -3 
Query:  257 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 655
            LTGRKII+DTY            GKDP+KVDRS AY  R  AK+IVA+GLA RC +QVSYAIGV EP S+ V+++GT K+P +++  +V+E FD RP  +   LDL    +  + +TAAYGHFGR+   F WE
Sbjct:  242 LTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFFDLRPYGLIQMLDLL---HPIYKETAAYGHFGRE--HFPWE 369
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK2_YEAST (S-adenosylmethionine synthetase 2 OS=Saccharomyces cerevisiae GN=SAM2 PE=1 SV=3)

HSP 1 Score: 126.716 bits (317), Expect = 1.041e-28
Identity = 70/140 (50.00%), Postives = 92/140 (65.71%), Query Frame = -3 
Query:  236 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKW 655
            LTGRKII+D Y            GKD +KVDRS AY  R  AKS+VA+GL +R  VQ SYAIG+ EPLS+ VD+YGT    D EI++I+K+NFD RPG++   LDL R     +L TA+YGHF   + +++WE  K L++
Sbjct:  250 LTGRKIIVDAYGGASSVGGGAFSGKDYSKVDRSAAYAARWVAKSLVAAGLCKRVQVQFSYAIGIAEPLSLHVDTYGTATKSDDEIIEIIKKNFDLRPGVLVKELDLAR---PIYLPTASYGHF--TNQEYSWEKPKKLEF 384
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_SYNSC (S-adenosylmethionine synthetase OS=Synechococcus sp. (strain CC9605) GN=metK PE=3 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 1.359e-28
Identity = 71/140 (50.71%), Postives = 90/140 (64.29%), Query Frame = -3 
Query:  251 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMI-----SINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIV 655
            LTGRKII+DTY            GKDPTKVDRS AY  R  AK +VA+GLA R  VQ+SYAIGV +P+S+ V+S+GT  + +  +  +V+E+FD RPG I       NL  +RGG   +  TAAYGHFGR+D +  WE V
Sbjct:  260 LTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKCLVAAGLAERAEVQLSYAIGVAKPVSILVESFGTSALANDALTALVQEHFDLRPGAIIETFGLRNLPQQRGG-CFYQDTAAYGHFGRNDLNAPWEDV 398
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_PEDPA (S-adenosylmethionine synthetase OS=Pediococcus pentosaceus (strain ATCC 25745 / 183-1w) GN=metK PE=3 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 1.359e-28
Identity = 71/145 (48.97%), Postives = 94/145 (64.83%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKII+DTY            GKD TKVDRS +Y  R  AK+IVA+ LA R  VQ++YAIGV EP+SV VD++GTG + ++++ + V++ FD RP  I   LDLKR     + +TAAYGHFGR D +  WE     K +K++A
Sbjct:  253 LTGRKIIVDTYGGAARHGGGAFSGKDATKVDRSASYAARYIAKNIVAAKLAERVEVQIAYAIGVAEPVSVMVDTFGTGTVSEEKLTQAVRKIFDLRPAGIIEMLDLKR---PIYKQTAAYGHFGRTDVELPWE-----KTDKVEA 389
The following BLAST results are available for this feature:
BLAST of CF835163 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_ECO7I1.041e-2851.88S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_ECO5E1.041e-2851.88S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_ECO571.041e-2851.88S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_ECO551.041e-2851.88S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_ECO271.041e-2851.88S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_ECO241.041e-2851.88S-adenosylmethionine synthetase OS=Escherichia col... [more]
METK_CITK81.041e-2851.88S-adenosylmethionine synthetase OS=Citrobacter kos... [more]
METK2_YEAST1.041e-2850.00S-adenosylmethionine synthetase 2 OS=Saccharomyces... [more]
METK_SYNSC1.359e-2850.71S-adenosylmethionine synthetase OS=Synechococcus s... [more]
METK_PEDPA1.359e-2848.97S-adenosylmethionine synthetase OS=Pediococcus pen... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06M13_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06M13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835163 ID=CF835163; Name=CF835163; organism=Citrus sinensis; type=EST; length=657bp
TTGGTTTACCCTTAAGAGAATTTCAGAACAATTCATATTCGCCTCTCCCC
CCATTATGCCTTGGATTGAGAATCCATTAAACATATTTATTAAAAAAAAA
GGGGAAAAGAAGAAGAAAGAAAGAAAGAAAACATTCATGTAACTGAAACA
GAAGTAAAAAAGAAAAGTAGACATATATAAGCACCAATGAGAGGCCAGCA
GCTCATCCTTGATTAGTTTAAGCTTGAAGTTTCTCCCACTTGAGTGGCTT
AACAATCTCCCAAGTGAAATCAGGATCATCTCTGCCAAAGTGCCCATAAG
CGGCAGTCTTCAAGAACCTGCCGTTGCCACCCCTCTTGAGATCAAGATTA
ATAGAAATCATCCCAGGCCTAAAATCAAAATTCTCCTTCACAATCTTCAA
AATTTCCTTGTCAGGGATCTTCCCAGTCCCATAACTATCAACAAAAACTG
ACAAAGGCTCGGGCACACCAATAGCGTAAGAAACCTGAACAATGCATCTC
CTGGCAAGCCCGCTTGCCACAATACTCTTAGCAGCTTGCCTTACAATGTA
AGCACCGCTTCTATCAACCTTGGTAGGATCTTTCCCTGAGAAAGCCCCAC
CACCGTGTGCCCCCCAGCCACCATAAGTATCAATAATAATCTTCCGGCCG
GTGAGCC
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