CF835163

Overview
NameCF835163
Unique NameCF835163
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length657
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CF835163_ssr113 microsatellite CF835163_ssr113:113..128. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig10947 contig Csv1_Contig10947:93..750. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK1_BETVU (S-adenosylmethionine synthetase 1 OS=Beta vulgaris GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 238.039 bits (606), Expect = 3.205e-62
Identity = 119/145 (82.07%), Postives = 128/145 (88.28%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYI RQAAKSIVASGLARRCIVQ+SYAIGVPEPLSVFVD+YGTGKIPDK+ILKIVKE FDFRPGMI+INLDL +GG+ R+LKTAAYGHFGRDDPDFTWE+VKPLKW+K QA
Sbjct:  253 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQAAKSIVASGLARRCIVQISYAIGVPEPLSVFVDTYGTGKIPDKDILKIVKETFDFRPGMIAINLDLLKGGS-RYLKTAAYGHFGRDDPDFTWEVVKPLKWDKPQA 396
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK1_PEA (S-adenosylmethionine synthetase 1 (Fragment) OS=Pisum sativum GN=SAMS1 PE=2 SV=2)

HSP 1 Score: 237.269 bits (604), Expect = 5.467e-62
Identity = 119/142 (83.80%), Postives = 124/142 (87.32%), Query Frame = -3 
Query:  230 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEK 655
            LTGRKIIIDTY            GKDPTKVDR GAYIVRQAAKSIVASGLARR IVQ+  AIGVPEPLSVFVD+YGTGKIPD+EILKIVKE FDFRPGMISINLDL RGGNGRFLKTAAYGHFGR+DPDFTWE+VKPLKWEK
Sbjct:  224 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRRGAYIVRQAAKSIVASGLARRAIVQLLRAIGVPEPLSVFVDTYGTGKIPDREILKIVKETFDFRPGMISINLDLLRGGNGRFLKTAAYGHFGREDPDFTWEVVKPLKWEK 365
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK2_HORVU (S-adenosylmethionine synthetase 2 OS=Hordeum vulgare GN=SAM2 PE=2 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 1.218e-61
Identity = 118/145 (81.38%), Postives = 127/145 (87.59%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYI RQAAKSI+ASGLARRCIVQ+SYAIGVPEPLSVFVDSYGTGKIPD+EILK+VKENFDFRPGMISINLDLK+GG  RF+KTAAYGHFGRDD DFTWE+VKPLK++K  A
Sbjct:  251 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQAAKSIIASGLARRCIVQISYAIGVPEPLSVFVDSYGTGKIPDREILKLVKENFDFRPGMISINLDLKKGGK-RFIKTAAYGHFGRDDADFTWEVVKPLKFDKASA 394
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK3_ATRNU (S-adenosylmethionine synthetase 3 OS=Atriplex nummularia GN=SAMS3 PE=1 SV=1)

HSP 1 Score: 234.958 bits (598), Expect = 2.713e-61
Identity = 119/145 (82.07%), Postives = 127/145 (87.59%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYI RQAAKSIVA+GLARRCIVQ+SYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE+FDFRPGMI+INLDL +GG+ R+LKTAAYGHFGRDD DFTWE VKPLKWEK QA
Sbjct:  253 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQAAKSIVAAGLARRCIVQISYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKESFDFRPGMIAINLDLLKGGS-RYLKTAAYGHFGRDDADFTWETVKPLKWEKPQA 396
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK1_HORVU (S-adenosylmethionine synthetase 1 OS=Hordeum vulgare GN=SAM1 PE=2 SV=1)

HSP 1 Score: 234.958 bits (598), Expect = 2.713e-61
Identity = 117/145 (80.69%), Postives = 127/145 (87.59%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LT RKIIIDTY            GKDPTKVDRSGAYI RQAAKSI+ASGLARRCIVQ+SYAIGVPEPLSVFVDSYGTGKIPD+EILK+VKENFDFRPGMI+INLDLK+GGN RF+KTAAYGHFGRDD DFTWE+VKPLK++K  A
Sbjct:  251 LTARKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQAAKSIIASGLARRCIVQISYAIGVPEPLSVFVDSYGTGKIPDREILKLVKENFDFRPGMITINLDLKKGGN-RFIKTAAYGHFGRDDADFTWEVVKPLKFDKASA 394
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK4_ATRNU (S-adenosylmethionine synthetase 4 OS=Atriplex nummularia GN=SAMS4 PE=1 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 3.544e-61
Identity = 119/145 (82.07%), Postives = 126/145 (86.90%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYI RQAAKSIVA+GLARRCIVQ+SYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE FDFRPGMI+INLDL +GG+ R+LKTAAYGHFGRDD DFTWE VKPLKWEK QA
Sbjct:  253 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQAAKSIVAAGLARRCIVQISYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKETFDFRPGMIAINLDLLKGGS-RYLKTAAYGHFGRDDADFTWETVKPLKWEKPQA 396
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK2_ATRNU (S-adenosylmethionine synthetase 2 OS=Atriplex nummularia GN=SAMS2 PE=1 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 3.544e-61
Identity = 119/145 (82.07%), Postives = 126/145 (86.90%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYI RQAAKSIVA+GLARRCIVQ+SYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE FDFRPGMI+INLDL +GG+ R+LKTAAYGHFGRDD DFTWE VKPLKWEK QA
Sbjct:  253 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQAAKSIVAAGLARRCIVQISYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKETFDFRPGMIAINLDLLKGGS-RYLKTAAYGHFGRDDADFTWETVKPLKWEKPQA 396
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK1_ATRNU (S-adenosylmethionine synthetase 1 OS=Atriplex nummularia GN=SAMS1 PE=1 SV=2)

HSP 1 Score: 234.572 bits (597), Expect = 3.544e-61
Identity = 119/145 (82.07%), Postives = 126/145 (86.90%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYI RQAAKSIVA+GLARRCIVQ+SYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE FDFRPGMI+INLDL +GG+ R+LKTAAYGHFGRDD DFTWE VKPLKWEK QA
Sbjct:  253 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIARQAAKSIVAAGLARRCIVQISYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKETFDFRPGMIAINLDLLKGGS-RYLKTAAYGHFGRDDADFTWETVKPLKWEKPQA 396
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK1_DAUCA (S-adenosylmethionine synthetase 1 OS=Daucus carota GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 1.031e-60
Identity = 128/193 (66.32%), Postives = 131/193 (67.88%), Query Frame = -3 
Query:  221 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAA------------------------------------------------YGHFGRDDPDFTWEIVKPLKWEKLQA 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPD+EIL+IVKE FDFRPGMISINLDLKRGGNGRFLKTAA                                                YGHFGRDDPDFTWE+VKPLKWEK QA
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDREILQIVKETFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGTGKIPDREILKIVKETFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWEVVKPLKWEKPQA 441
[Close]
BLAST of CF835163 vs. ExPASy Swiss-Prot
Match: METK_CAMSI (S-adenosylmethionine synthetase OS=Camellia sinensis GN=SAM PE=2 SV=1)

HSP 1 Score: 231.876 bits (590), Expect = 2.297e-60
Identity = 116/133 (87.22%), Postives = 120/133 (90.23%), Query Frame = -3 
Query:  257 LTGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKEILKIVKENFDFRPGMISINLDLKRGGNGRFLKTAAYGHFGRDDPDFTWE 655
            LTGRKIIIDTY            GKDPTKVDRSGAYIVRQAAKSIVA+GLARRCIVQVSYAIGVPEPLSVFVD+YGTGKIPDKEILKIVKE+FDFRPGMI+INLDLKRGGN RFLKTAAYGHFGRDDPDFTWE
Sbjct:  249 LTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILKIVKESFDFRPGMIAINLDLKRGGNSRFLKTAAYGHFGRDDPDFTWE 381
The following BLAST results are available for this feature:
BLAST of CF835163 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK1_BETVU3.205e-6282.07S-adenosylmethionine synthetase 1 OS=Beta vulgaris... [more]
METK1_PEA5.467e-6283.80S-adenosylmethionine synthetase 1 (Fragment) OS=Pi... [more]
METK2_HORVU1.218e-6181.38S-adenosylmethionine synthetase 2 OS=Hordeum vulga... [more]
METK3_ATRNU2.713e-6182.07S-adenosylmethionine synthetase 3 OS=Atriplex numm... [more]
METK1_HORVU2.713e-6180.69S-adenosylmethionine synthetase 1 OS=Hordeum vulga... [more]
METK4_ATRNU3.544e-6182.07S-adenosylmethionine synthetase 4 OS=Atriplex numm... [more]
METK2_ATRNU3.544e-6182.07S-adenosylmethionine synthetase 2 OS=Atriplex numm... [more]
METK1_ATRNU3.544e-6182.07S-adenosylmethionine synthetase 1 OS=Atriplex numm... [more]
METK1_DAUCA1.031e-6066.32S-adenosylmethionine synthetase 1 OS=Daucus carota... [more]
METK_CAMSI2.297e-6087.22S-adenosylmethionine synthetase OS=Camellia sinens... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06M13_f Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06M13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835163 ID=CF835163; Name=CF835163; organism=Citrus sinensis; type=EST; length=657bp
TTGGTTTACCCTTAAGAGAATTTCAGAACAATTCATATTCGCCTCTCCCC
CCATTATGCCTTGGATTGAGAATCCATTAAACATATTTATTAAAAAAAAA
GGGGAAAAGAAGAAGAAAGAAAGAAAGAAAACATTCATGTAACTGAAACA
GAAGTAAAAAAGAAAAGTAGACATATATAAGCACCAATGAGAGGCCAGCA
GCTCATCCTTGATTAGTTTAAGCTTGAAGTTTCTCCCACTTGAGTGGCTT
AACAATCTCCCAAGTGAAATCAGGATCATCTCTGCCAAAGTGCCCATAAG
CGGCAGTCTTCAAGAACCTGCCGTTGCCACCCCTCTTGAGATCAAGATTA
ATAGAAATCATCCCAGGCCTAAAATCAAAATTCTCCTTCACAATCTTCAA
AATTTCCTTGTCAGGGATCTTCCCAGTCCCATAACTATCAACAAAAACTG
ACAAAGGCTCGGGCACACCAATAGCGTAAGAAACCTGAACAATGCATCTC
CTGGCAAGCCCGCTTGCCACAATACTCTTAGCAGCTTGCCTTACAATGTA
AGCACCGCTTCTATCAACCTTGGTAGGATCTTTCCCTGAGAAAGCCCCAC
CACCGTGTGCCCCCCAGCCACCATAAGTATCAATAATAATCTTCCGGCCG
GTGAGCC
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