CF835164

Overview
NameCF835164
Unique NameCF835164
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length548
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CF835164_ssr33 microsatellite CF835164_ssr33:33..42. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig12594 contig Csv1_Contig12594:11..558. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK2_VITVI (S-adenosylmethionine synthetase 2 OS=Vitis vinifera GN=METK2 PE=3 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 7.061e-61
Identity = 112/119 (94.12%), Postives = 114/119 (95.80%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDPDSKVACETCTKTNM MVFGEITTKANVDYEKIVRDTCR IGF S DVGLDAD+CKVLVNIEQQSPDIAQGVHGHLTKRPEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLQQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCREIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK2_PETHY (S-adenosylmethionine synthetase 2 OS=Petunia hybrida GN=SAM2 PE=2 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 7.061e-61
Identity = 111/119 (93.28%), Postives = 115/119 (96.64%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQVSDA+LDACL QDP+SKVACETCTKT+M MVFGEITTKA VDYEKIVRDTCR IGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTK+PEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTSMVMVFGEITTKATVDYEKIVRDTCRGIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKKPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK_POPDE (S-adenosylmethionine synthetase OS=Populus deltoides GN=METK PE=2 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 9.223e-61
Identity = 111/118 (94.07%), Postives = 116/118 (98.31%), Query Frame = 1 
Query:  193 ETFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            ETFLFTSESVNEGHPDKLCDQ+SDAVLDACLAQDPDSKVACETCTKTNM MVFGEITTKA+VDYEKIVRDTCR+IGFTSADVGLDAD+CKVLVNIEQQSPDIAQGVHGH +KRPEEIG
Sbjct:    3 ETFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKADVDYEKIVRDTCRNIGFTSADVGLDADNCKVLVNIEQQSPDIAQGVHGHFSKRPEEIG 120
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK3_POPTR (S-adenosylmethionine synthetase 3 OS=Populus trichocarpa GN=METK3 PE=2 SV=1)

HSP 1 Score: 232.646 bits (592), Expect = 9.223e-61
Identity = 111/118 (94.07%), Postives = 116/118 (98.31%), Query Frame = 1 
Query:  193 ETFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            ETFLFTSESVNEGHPDKLCDQ+SDAVLDACLAQDPDSKVACETCTKTNM MVFGEITTKA+VDYEKIVRDTCR+IGFTSADVGLDAD+CKVLVNIEQQSPDIAQGVHGH +KRPEEIG
Sbjct:    3 ETFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKADVDYEKIVRDTCRNIGFTSADVGLDADNCKVLVNIEQQSPDIAQGVHGHFSKRPEEIG 120
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK_WHEAT (S-adenosylmethionine synthetase OS=Triticum aestivum GN=SAMS PE=2 SV=1)

HSP 1 Score: 232.261 bits (591), Expect = 1.205e-60
Identity = 110/119 (92.44%), Postives = 114/119 (95.80%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            ++TFLFTSESVNEGHPDKLCDQ+SDAVLDACLA+DPDSKVACETCTKTNM MVFGEITTKANVDYEKIVRDTCR IGF S DVGLDADHCKVLVNIEQQSPDIAQGVHGH TKRPEEIG
Sbjct:    4 VDTFLFTSESVNEGHPDKLCDQISDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRGIGFVSNDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIG 122
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK_CARPA (S-adenosylmethionine synthetase OS=Carica papaya GN=SAMS PE=2 SV=1)

HSP 1 Score: 232.261 bits (591), Expect = 1.205e-60
Identity = 112/119 (94.12%), Postives = 114/119 (95.80%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACLAQDPDSKVACETCTKTNM MVFGEITTKA+VDYEKIVRDTCRSIGF S DVGLDAD CKVLVNIEQQSPDIAQGVHGH TKRPEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKADVDYEKIVRDTCRSIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK2_ELAUM (S-adenosylmethionine synthetase 2 OS=Elaeagnus umbellata GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 232.261 bits (591), Expect = 1.205e-60
Identity = 111/119 (93.28%), Postives = 115/119 (96.64%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACLAQDPDSKVACETC+KTNM MVFGEITTKANVDYEKIVRDTCR+IGF S DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TKRPEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVACETCSKTNMVMVFGEITTKANVDYEKIVRDTCRTIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHFTKRPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK1_TOBAC (S-adenosylmethionine synthetase 1 OS=Nicotiana tabacum GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 232.261 bits (591), Expect = 1.205e-60
Identity = 111/119 (93.28%), Postives = 115/119 (96.64%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQVSDA+LDACL QDP+SKVACETCTKTNM MVFGEITTKA VDYEKIVRDTCR IGFTSADVGLDAD+CKVLVNIEQQSPDIAQGVHGHLTK+PEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRGIGFTSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK1_ELAUM (S-adenosylmethionine synthetase 1 OS=Elaeagnus umbellata GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 232.261 bits (591), Expect = 1.205e-60
Identity = 111/119 (93.28%), Postives = 115/119 (96.64%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACLAQDPDSKVACETC+KTNM MVFGEITTKANVDYEKIVRDTCR+IGF S DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TKRPEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVACETCSKTNMVMVFGEITTKANVDYEKIVRDTCRAIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHFTKRPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK1_CATRO (S-adenosylmethionine synthetase 1 OS=Catharanthus roseus GN=SAMS1 PE=1 SV=1)

HSP 1 Score: 232.261 bits (591), Expect = 1.205e-60
Identity = 112/119 (94.12%), Postives = 114/119 (95.80%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDPDSKVACETCTKTNM MVFGEITTKA VDYEKIVRDTCRSIGF S DVGLDAD+CKVLVNIEQQSPDIAQGVHGHLTKRPEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRSIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 119
The following BLAST results are available for this feature:
BLAST of CF835164 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK2_VITVI7.061e-6194.12S-adenosylmethionine synthetase 2 OS=Vitis vinifer... [more]
METK2_PETHY7.061e-6193.28S-adenosylmethionine synthetase 2 OS=Petunia hybri... [more]
METK_POPDE9.223e-6194.07S-adenosylmethionine synthetase OS=Populus deltoid... [more]
METK3_POPTR9.223e-6194.07S-adenosylmethionine synthetase 3 OS=Populus trich... [more]
METK_WHEAT1.205e-6092.44S-adenosylmethionine synthetase OS=Triticum aestiv... [more]
METK_CARPA1.205e-6094.12S-adenosylmethionine synthetase OS=Carica papaya G... [more]
METK2_ELAUM1.205e-6093.28S-adenosylmethionine synthetase 2 OS=Elaeagnus umb... [more]
METK1_TOBAC1.205e-6093.28S-adenosylmethionine synthetase 1 OS=Nicotiana tab... [more]
METK1_ELAUM1.205e-6093.28S-adenosylmethionine synthetase 1 OS=Elaeagnus umb... [more]
METK1_CATRO1.205e-6094.12S-adenosylmethionine synthetase 1 OS=Catharanthus ... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06M13_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06M13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835164 ID=CF835164; Name=CF835164; organism=Citrus sinensis; type=EST; length=548bp
GAAACTACAAATCCTTGCTGCCGTTTTACCTCCTCTCTCTCTTCTCTCAG
CGTATGCATATCGGCTTTCTCTCGTTCTTCTGGGTACTTACCATTTTCGT
ATATCACCACCTTCAATTCCATTTGTACTCGCACCACGGCCTCCTGCAAA
GCGAAAGCTGCAGCTTCAGGTTAAATCAAATTAAAGAAAATGGAAACATT
TCTTTTCACATCAGAATCAGTGAACGAGGGTCACCCGGACAAGCTGTGTG
ACCAAGTTTCGGACGCAGTACTGGACGCGTGTTTGGCTCAGGATCCTGAC
AGCAAAGTTGCTTGTGAAACATGCACAAAGACCAACATGGACATGGTTTT
TGGAGAAATTACAACCAAGGCCAATGTGGATTACGAGAAAATCGTGCGTG
ATACATGCAGATCGATTGGGTTCACATCGGCCGATGTGGGGCTTGATGCT
GATCACTGCAAAGTTCTTGTTAATATTGAGCAGCAAAGCCCTGACATTGC
TCAGGGTGTTCATGGGCATCTCACAAAGCGGCCTGAGGAGATTGGTGC
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