CF835164

Overview
NameCF835164
Unique NameCF835164
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length548
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CF835164_ssr33 microsatellite CF835164_ssr33:33..42. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig12594 contig Csv1_Contig12594:11..558. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK5_BRAJU (S-adenosylmethionine synthetase 5 OS=Brassica juncea GN=MSAMS5 PE=2 SV=1)

HSP 1 Score: 228.409 bits (581), Expect = 1.739e-59
Identity = 110/119 (92.44%), Postives = 112/119 (94.12%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDPDSKVACETCTKTNM MVFGEITTKA VDYEKIVRDTCRSIGF S DVGLDAD CKVLVNIEQQSPDIAQGVHGH TKRPE+IG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK3_CATRO (S-adenosylmethionine synthetase 3 OS=Catharanthus roseus GN=SAMS3 PE=1 SV=1)

HSP 1 Score: 228.409 bits (581), Expect = 1.739e-59
Identity = 109/119 (91.60%), Postives = 114/119 (95.80%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQVSDA+LDACL QDP+SKVACETCTKTNM MVFGEITTKA V+YEKIVRDTCR IGFTS DVGLDAD+CKVLVNIEQQSPDIAQGVHGHLTK+PEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKATVNYEKIVRDTCRGIGFTSPDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK3_BRAJU (S-adenosylmethionine synthetase 3 OS=Brassica juncea GN=MSAMS3 PE=2 SV=1)

HSP 1 Score: 228.409 bits (581), Expect = 1.739e-59
Identity = 110/119 (92.44%), Postives = 112/119 (94.12%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDPDSKVACETCTKTNM MVFGEITTKA VDYEKIVRDTCRSIGF S DVGLDAD CKVLVNIEQQSPDIAQGVHGH TKRPE+IG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK3_ATRNU (S-adenosylmethionine synthetase 3 OS=Atriplex nummularia GN=SAMS3 PE=1 SV=1)

HSP 1 Score: 228.409 bits (581), Expect = 1.739e-59
Identity = 108/119 (90.76%), Postives = 115/119 (96.64%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            ++TFLFTSESVNEGHPDKLCDQ+SDAVLDACLAQDP+SKVACETCTKTN+ MVFGEITTKANVDYEKIVR TCR IGF SADVGLDAD+CKVLVNIEQQSPDIAQGVHGHLT+RPEEIG
Sbjct:    5 VDTFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPESKVACETCTKTNLVMVFGEITTKANVDYEKIVRQTCRDIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTRRPEEIG 123
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK1_BRAJU (S-adenosylmethionine synthetase 1 OS=Brassica juncea GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 228.409 bits (581), Expect = 1.739e-59
Identity = 110/119 (92.44%), Postives = 112/119 (94.12%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDPDSKVACETCTKTNM MVFGEITTKA VDYEKIVRDTCRSIGF S DVGLDAD CKVLVNIEQQSPDIAQGVHGH TKRPE+IG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK1_ACTCH (S-adenosylmethionine synthetase 1 OS=Actinidia chinensis GN=SAM1 PE=2 SV=1)

HSP 1 Score: 228.409 bits (581), Expect = 1.739e-59
Identity = 108/119 (90.76%), Postives = 114/119 (95.80%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            M+TFLFTSESVNEGHPDKLCDQVSDA+LDACL QDP+SKVACE+CTKTNM MVFGEITTKA V+YEKIVRDTCR IGFTS DVGLDADHCKVLVNIEQQSPDIAQGVHGHLTK+PEEIG
Sbjct:    1 MDTFLFTSESVNEGHPDKLCDQVSDAILDACLKQDPESKVACESCTKTNMVMVFGEITTKAQVNYEKIVRDTCRGIGFTSPDVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKKPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK3_PICSI (S-adenosylmethionine synthetase 3 OS=Picea sitchensis GN=METK3 PE=2 SV=1)

HSP 1 Score: 228.024 bits (580), Expect = 2.272e-59
Identity = 109/119 (91.60%), Postives = 112/119 (94.12%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDPDSKVACETCTKTNM MVFGEITTKA+VDYE+IVR TCR IGF S DVGLDADHCKVLVNIEQQSPDIAQGVHGH TKRPEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLTQDPDSKVACETCTKTNMVMVFGEITTKADVDYEQIVRKTCREIGFISDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK2_PINCO (S-adenosylmethionine synthetase 2 OS=Pinus contorta GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 228.024 bits (580), Expect = 2.272e-59
Identity = 109/119 (91.60%), Postives = 112/119 (94.12%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDPDSKVACETCTKTNM MVFGEITTKA+VDYE+IVR TCR IGF S DVGLDADHCKVLVNIEQQSPDIAQGVHGH TKRPEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLTQDPDSKVACETCTKTNMVMVFGEITTKADVDYEQIVRKTCREIGFISDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK2_ARATH (S-adenosylmethionine synthetase 2 OS=Arabidopsis thaliana GN=SAM2 PE=1 SV=1)

HSP 1 Score: 228.024 bits (580), Expect = 2.272e-59
Identity = 109/119 (91.60%), Postives = 112/119 (94.12%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDPDSKVACETCTKTNM MVFGEITTKA +DYEKIVRDTCRSIGF S DVGLDAD CKVLVNIEQQSPDIAQGVHGH TKRPE+IG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATIDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK2_ACTCH (S-adenosylmethionine synthetase 2 OS=Actinidia chinensis GN=SAM2 PE=2 SV=1)

HSP 1 Score: 228.024 bits (580), Expect = 2.272e-59
Identity = 108/119 (90.76%), Postives = 114/119 (95.80%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            ME+FLFTSESVNEGHPDKLCDQVSDA+LDACL QDP+SKVACETCTKTNM MVFGEITTKA V+YEKIVRDTCR IGFTS DVGLDADHCKVLVNIEQQSPDIAQGVHGHL+K+PEEIG
Sbjct:    1 MESFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVNYEKIVRDTCRGIGFTSPDVGLDADHCKVLVNIEQQSPDIAQGVHGHLSKKPEEIG 119
The following BLAST results are available for this feature:
BLAST of CF835164 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK5_BRAJU1.739e-5992.44S-adenosylmethionine synthetase 5 OS=Brassica junc... [more]
METK3_CATRO1.739e-5991.60S-adenosylmethionine synthetase 3 OS=Catharanthus ... [more]
METK3_BRAJU1.739e-5992.44S-adenosylmethionine synthetase 3 OS=Brassica junc... [more]
METK3_ATRNU1.739e-5990.76S-adenosylmethionine synthetase 3 OS=Atriplex numm... [more]
METK1_BRAJU1.739e-5992.44S-adenosylmethionine synthetase 1 OS=Brassica junc... [more]
METK1_ACTCH1.739e-5990.76S-adenosylmethionine synthetase 1 OS=Actinidia chi... [more]
METK3_PICSI2.272e-5991.60S-adenosylmethionine synthetase 3 OS=Picea sitchen... [more]
METK2_PINCO2.272e-5991.60S-adenosylmethionine synthetase 2 OS=Pinus contort... [more]
METK2_ARATH2.272e-5991.60S-adenosylmethionine synthetase 2 OS=Arabidopsis t... [more]
METK2_ACTCH2.272e-5990.76S-adenosylmethionine synthetase 2 OS=Actinidia chi... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06M13_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06M13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835164 ID=CF835164; Name=CF835164; organism=Citrus sinensis; type=EST; length=548bp
GAAACTACAAATCCTTGCTGCCGTTTTACCTCCTCTCTCTCTTCTCTCAG
CGTATGCATATCGGCTTTCTCTCGTTCTTCTGGGTACTTACCATTTTCGT
ATATCACCACCTTCAATTCCATTTGTACTCGCACCACGGCCTCCTGCAAA
GCGAAAGCTGCAGCTTCAGGTTAAATCAAATTAAAGAAAATGGAAACATT
TCTTTTCACATCAGAATCAGTGAACGAGGGTCACCCGGACAAGCTGTGTG
ACCAAGTTTCGGACGCAGTACTGGACGCGTGTTTGGCTCAGGATCCTGAC
AGCAAAGTTGCTTGTGAAACATGCACAAAGACCAACATGGACATGGTTTT
TGGAGAAATTACAACCAAGGCCAATGTGGATTACGAGAAAATCGTGCGTG
ATACATGCAGATCGATTGGGTTCACATCGGCCGATGTGGGGCTTGATGCT
GATCACTGCAAAGTTCTTGTTAATATTGAGCAGCAAAGCCCTGACATTGC
TCAGGGTGTTCATGGGCATCTCACAAAGCGGCCTGAGGAGATTGGTGC
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