CF835164

Overview
NameCF835164
Unique NameCF835164
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length548
Libraries
Library NameType
Ruby Orange Ovary at Anthesis cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CF835164_ssr33 microsatellite CF835164_ssr33:33..42. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig12594 contig Csv1_Contig12594:11..558. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK_CAMSI (S-adenosylmethionine synthetase OS=Camellia sinensis GN=SAM PE=2 SV=1)

HSP 1 Score: 227.254 bits (578), Expect = 3.875e-59
Identity = 110/119 (92.44%), Postives = 113/119 (94.96%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACL QD DSKVACETCTKTNM MVFGEITTKA VDYEKIVRDTCR+IGF S DVGLDAD+CKVLVNIEQQSPDIAQGVHGHLTKRPEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDQDSKVACETCTKTNMVMVFGEITTKAAVDYEKIVRDTCRTIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK2_PICSI (S-adenosylmethionine synthetase 2 OS=Picea sitchensis GN=METK2 PE=2 SV=1)

HSP 1 Score: 227.254 bits (578), Expect = 3.875e-59
Identity = 108/119 (90.76%), Postives = 112/119 (94.12%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDPDSKVACETCTKTNM MVFGEITTKA++DYE+IVR TCR IGF S DVGLDADHCKVLVNIEQQSPDIAQGVHGH TKRPEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLIQDPDSKVACETCTKTNMVMVFGEITTKADIDYEQIVRKTCREIGFISDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK1_VITVI (S-adenosylmethionine synthetase 1 OS=Vitis vinifera GN=METK1 PE=3 SV=1)

HSP 1 Score: 227.254 bits (578), Expect = 3.875e-59
Identity = 108/119 (90.76%), Postives = 114/119 (95.80%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            M+TFLFTSESVNEGHPDKLCDQVSDA+LDACL QDP+SKVACETCTKTNM MVFGEITTKA V+YEKIVRDTCR IGF SADVGLDAD+CKVLVNIEQQSPDIAQGVHGHLTK+PEEIG
Sbjct:    1 MDTFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVNYEKIVRDTCRGIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK_DENCR (S-adenosylmethionine synthetase OS=Dendrobium crumenatum GN=SAMS PE=2 SV=1)

HSP 1 Score: 226.868 bits (577), Expect = 5.061e-59
Identity = 106/119 (89.08%), Postives = 114/119 (95.80%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            ++TFLFTSESVNEGHPDKLCDQ+SDA+LDACL QDPDSKVACETC+KTNM M+FGEITTKANVDYEKIVRDTCR+IGF S DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TKRPEEIG
Sbjct:    4 VDTFLFTSESVNEGHPDKLCDQISDAILDACLEQDPDSKVACETCSKTNMVMIFGEITTKANVDYEKIVRDTCRAIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHFTKRPEEIG 122
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK2_BRAJU (S-adenosylmethionine synthetase 2 OS=Brassica juncea GN=MSAMS2 PE=2 SV=1)

HSP 1 Score: 226.483 bits (576), Expect = 6.609e-59
Identity = 107/119 (89.92%), Postives = 114/119 (95.80%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            ME+FLFTSESVNEGHPDKLCDQ+SDA+LDACL QDP+SKVACETCTKTNM MVFGEITTKANVDYEKIVR+TCR IGF S DVGLDAD+CKVLVNIEQQSPDIAQGVHGHLTK+PEEIG
Sbjct:    1 MESFLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANVDYEKIVRETCREIGFISDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK1_POPTR (S-adenosylmethionine synthetase 1 OS=Populus trichocarpa GN=METK1 PE=2 SV=1)

HSP 1 Score: 226.483 bits (576), Expect = 6.609e-59
Identity = 108/119 (90.76%), Postives = 111/119 (93.28%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDPDSKVACETCTKTNM MVFGEITTK  +DYEKIVRDTCR+IGF S DVGLDAD CKVLVNIEQQSPDIAQGVHGH TKRPEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKGKIDYEKIVRDTCRNIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK4_ARATH (S-adenosylmethionine synthetase 4 OS=Arabidopsis thaliana GN=METK4 PE=1 SV=1)

HSP 1 Score: 226.098 bits (575), Expect = 8.632e-59
Identity = 106/119 (89.08%), Postives = 114/119 (95.80%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            ME+FLFTSESVNEGHPDKLCDQ+SDA+LDACL QDP+SKVACETCTKTNM MVFGEITTKANVDYE+IVR TCR IGF SADVGLDAD+CKVLVNIEQQSPDIAQGVHGHLTK+PEE+G
Sbjct:    1 MESFLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANVDYEQIVRKTCREIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEVG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK3_VITVI (S-adenosylmethionine synthetase 3 OS=Vitis vinifera GN=METK3 PE=3 SV=1)

HSP 1 Score: 226.098 bits (575), Expect = 8.632e-59
Identity = 107/119 (89.92%), Postives = 114/119 (95.80%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            M+TFLFTSESVNEGHPDKLCDQVSDA+LDACL QDP+SKVACETC+KTNM MVFGEITTKA VDYEKIVRDTCR IGF SADVGLDAD+CKVLVNIEQQSPDIAQGVHGHL+K+PEEIG
Sbjct:    1 MDTFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCSKTNMVMVFGEITTKAKVDYEKIVRDTCRGIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLSKKPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK3_PETHY (S-adenosylmethionine synthetase 3 OS=Petunia hybrida GN=SAM3 PE=2 SV=1)

HSP 1 Score: 225.713 bits (574), Expect = 1.127e-58
Identity = 108/119 (90.76%), Postives = 114/119 (95.80%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDP+SKVACETCTKTN+ MVFGEITTKANVDYEKIVRDTCR+IGF S DVGLDAD+CKVLV IEQQSPDIAQGVHGHLTK+PEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNLVMVFGEITTKANVDYEKIVRDTCRNIGFISDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLTKQPEEIG 119
[Close]
BLAST of CF835164 vs. ExPASy Swiss-Prot
Match: METK1_ARATH (S-adenosylmethionine synthetase 1 OS=Arabidopsis thaliana GN=SAM1 PE=1 SV=2)

HSP 1 Score: 225.713 bits (574), Expect = 1.127e-58
Identity = 109/119 (91.60%), Postives = 111/119 (93.28%), Query Frame = 1 
Query:  190 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMDMVFGEITTKANVDYEKIVRDTCRSIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKRPEEIG 546
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDPDSKVACETCTKTNM MVFGEITTKA VDYEKIVRDTCR+IGF S DVGLDAD CKVLVNIEQQSPDIAQGVHGH TK PEEIG
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKCPEEIG 119
The following BLAST results are available for this feature:
BLAST of CF835164 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_CAMSI3.875e-5992.44S-adenosylmethionine synthetase OS=Camellia sinens... [more]
METK2_PICSI3.875e-5990.76S-adenosylmethionine synthetase 2 OS=Picea sitchen... [more]
METK1_VITVI3.875e-5990.76S-adenosylmethionine synthetase 1 OS=Vitis vinifer... [more]
METK_DENCR5.061e-5989.08S-adenosylmethionine synthetase OS=Dendrobium crum... [more]
METK2_BRAJU6.609e-5989.92S-adenosylmethionine synthetase 2 OS=Brassica junc... [more]
METK1_POPTR6.609e-5990.76S-adenosylmethionine synthetase 1 OS=Populus trich... [more]
METK4_ARATH8.632e-5989.08S-adenosylmethionine synthetase 4 OS=Arabidopsis t... [more]
METK3_VITVI8.632e-5989.92S-adenosylmethionine synthetase 3 OS=Vitis vinifer... [more]
METK3_PETHY1.127e-5890.76S-adenosylmethionine synthetase 3 OS=Petunia hybri... [more]
METK1_ARATH1.127e-5891.60S-adenosylmethionine synthetase 1 OS=Arabidopsis t... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS02_06M13_r Ruby Orange Ovary at Anthesis cDNA Library Citrus sinensis cDNA clone CS_REa06M13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CF835164 ID=CF835164; Name=CF835164; organism=Citrus sinensis; type=EST; length=548bp
GAAACTACAAATCCTTGCTGCCGTTTTACCTCCTCTCTCTCTTCTCTCAG
CGTATGCATATCGGCTTTCTCTCGTTCTTCTGGGTACTTACCATTTTCGT
ATATCACCACCTTCAATTCCATTTGTACTCGCACCACGGCCTCCTGCAAA
GCGAAAGCTGCAGCTTCAGGTTAAATCAAATTAAAGAAAATGGAAACATT
TCTTTTCACATCAGAATCAGTGAACGAGGGTCACCCGGACAAGCTGTGTG
ACCAAGTTTCGGACGCAGTACTGGACGCGTGTTTGGCTCAGGATCCTGAC
AGCAAAGTTGCTTGTGAAACATGCACAAAGACCAACATGGACATGGTTTT
TGGAGAAATTACAACCAAGGCCAATGTGGATTACGAGAAAATCGTGCGTG
ATACATGCAGATCGATTGGGTTCACATCGGCCGATGTGGGGCTTGATGCT
GATCACTGCAAAGTTCTTGTTAATATTGAGCAGCAAAGCCCTGACATTGC
TCAGGGTGTTCATGGGCATCTCACAAAGCGGCCTGAGGAGATTGGTGC
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