CK739901 | Citrus Genome Database

Overview


Name	CK739901
Unique Name	CK739901
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	290

Libraries


Library Name	Type
Ridge pineapple sweet orange entire seedling	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: UBC5_YEAST (Ubiquitin-conjugating enzyme E2-16 kDa OS=Saccharomyces cerevisiae GN=UBC5 PE=1 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 6.428e-19
Identity = 30/49 (61.22%), Postives = 38/49 (77.55%), Query Frame = 3

Query:   84 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPF**PHMQEVY 230
            +SKRI KEL DL +DPP SCSAGPV +D++HWQA+IMGP   P+   V+
Sbjct:    3 SSKRIAKELSDLGRDPPASCSAGPVGDDLYHWQASIMGPSDSPYAGGVF 51

HSP 2 Score: 45.0542 bits (105), Expect = 6.428e-19
Identity = 17/24 (70.83%), Postives = 21/24 (87.50%), Query Frame = 2

Query:  209 APYAGGVFLVTIHFPPDYPFQAPK 280
            +PYAGGVF ++IHFP DYPF+ PK
Sbjct:   44 SPYAGGVFFLSIHFPTDYPFKPPK 67

[Close]

BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: UBC4_CANAL (Ubiquitin-conjugating enzyme E2 4 OS=Candida albicans GN=UBC4 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 6.837e-18
Identity = 28/40 (70.00%), Postives = 35/40 (87.50%), Query Frame = 3

Query:   81 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP 200
            M+ KRI KEL DL +DPP+SCSAGPV +D++HWQA+IMGP
Sbjct:    1 MSLKRINKELSDLGRDPPSSCSAGPVGDDLYHWQASIMGP 40

HSP 2 Score: 42.743 bits (99), Expect = 6.837e-18
Identity = 16/24 (66.67%), Postives = 20/24 (83.33%), Query Frame = 2

Query:  209 APYAGGVFLVTIHFPPDYPFQAPK 280
            +PYAGGVF ++IHFP DYP + PK
Sbjct:   43 SPYAGGVFFLSIHFPTDYPLKPPK 66

[Close]

BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: U2D2B_RAT (Ubiquitin-conjugating enzyme E2 D2B OS=Rattus norvegicus GN=Ube2d2b PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 8.890e-18
Identity = 32/40 (80.00%), Postives = 33/40 (82.50%), Query Frame = 3

Query:   81 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP 200
            MA KRI KEL DL +DPP  CSAGPV EDMFHWQATIMGP
Sbjct:    1 MALKRIHKELNDLAQDPPAQCSAGPVGEDMFHWQATIMGP 40

HSP 2 Score: 38.1206 bits (87), Expect = 8.890e-18
Identity = 14/24 (58.33%), Postives = 18/24 (75.00%), Query Frame = 2

Query:  209 APYAGGVFLVTIHFPPDYPFQAPK 280
            +PY GG F +TI FP +YPF+ PK
Sbjct:   43 SPYQGGAFFLTIDFPTEYPFKPPK 66

[Close]

BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: U2D2B_MOUSE (Ubiquitin-conjugating enzyme E2 D2B OS=Mus musculus GN=Ube2d2b PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 8.890e-18
Identity = 32/40 (80.00%), Postives = 33/40 (82.50%), Query Frame = 3

Query:   81 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP 200
            MA KRI KEL DL +DPP  CSAGPV EDMFHWQATIMGP
Sbjct:    1 MALKRIHKELNDLAQDPPAQCSAGPVGEDMFHWQATIMGP 40

HSP 2 Score: 38.1206 bits (87), Expect = 8.890e-18
Identity = 14/24 (58.33%), Postives = 18/24 (75.00%), Query Frame = 2

Query:  209 APYAGGVFLVTIHFPPDYPFQAPK 280
            +PY GG F +TI FP +YPF+ PK
Sbjct:   43 SPYQGGAFFLTIDFPTEYPFKPPK 66

[Close]

BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: UBC12_ARATH (Probable ubiquitin-conjugating enzyme E2 12 OS=Arabidopsis thaliana GN=UBC12 PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 3.304e-17
Identity = 33/51 (64.71%), Postives = 41/51 (80.39%), Query Frame = 3

Query:   81 MASKRILKELKDLQKDPPTSCSAGPVA-EDMFHWQATIMGPF**PHMQEVY 230
            MASKRI +EL+D+Q+ PP +CSAGPVA ED+FHWQATIMGP   P+   V+
Sbjct:    1 MASKRISRELRDMQRHPPANCSAGPVAEEDIFHWQATIMGPHDSPYSGGVF 51

HSP 2 Score: 38.1206 bits (87), Expect = 3.304e-17
Identity = 15/24 (62.50%), Postives = 19/24 (79.17%), Query Frame = 2

Query:  209 APYAGGVFLVTIHFPPDYPFQAPK 280
            +PY+GGVF V+I F  DYPF+ PK
Sbjct:   44 SPYSGGVFTVSIDFSSDYPFKPPK 67

[Close]

BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: COP10_ARATH (Constitutive photomorphogenesis protein 10 OS=Arabidopsis thaliana GN=COP10 PE=1 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 7.283e-12
Identity = 20/39 (51.28%), Postives = 29/39 (74.36%), Query Frame = 3

Query:   84 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP 200
            + KRI +E+ +L  DPP  CSAGP  ++++HW ATI+GP
Sbjct:   37 SGKRIQREMAELNIDPPPDCSAGPKGDNLYHWIATIIGP 75

HSP 2 Score: 37.7354 bits (86), Expect = 7.283e-12
Identity = 15/26 (57.69%), Postives = 18/26 (69.23%), Query Frame = 2

Query:  212 PYAGGVFLVTIHFPPDYPFQAPKGCF 289
            PY GG+F + I FP DYPF+ PK  F
Sbjct:   79 PYEGGIFFLDIIFPSDYPFKPPKLVF 104

[Close]

BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: UBCD2_DROME (Ubiquitin-conjugating enzyme E2-24 kDa OS=Drosophila melanogaster GN=UbcD2 PE=1 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 1.217e-11
Identity = 19/39 (48.72%), Postives = 30/39 (76.92%), Query Frame = 3

Query:   84 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP 200
            ++KRI KEL ++  DPP +CSAGP  ++++ W +TI+GP
Sbjct:   87 SAKRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGP 125

HSP 2 Score: 38.5058 bits (88), Expect = 1.217e-11
Identity = 15/25 (60.00%), Postives = 18/25 (72.00%), Query Frame = 2

Query:  215 YAGGVFLVTIHFPPDYPFQAPKGCF 289
            Y GGVF + IHF P+YPF+ PK  F
Sbjct:  130 YEGGVFFLDIHFSPEYPFKPPKVTF 154

[Close]

BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: UB2E2_MOUSE (Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus GN=Ube2e2 PE=2 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 3.476e-11
Identity = 19/39 (48.72%), Postives = 31/39 (79.49%), Query Frame = 3

Query:   84 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP 200
            ++KRI KEL ++  DPP +CSAGP  ++++ W++TI+GP
Sbjct:   56 SAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGP 94

HSP 2 Score: 36.1946 bits (82), Expect = 3.476e-11
Identity = 15/25 (60.00%), Postives = 17/25 (68.00%), Query Frame = 2

Query:  215 YAGGVFLVTIHFPPDYPFQAPKGCF 289
            Y GGVF + I F PDYPF+ PK  F
Sbjct:   99 YEGGVFFLDITFSPDYPFKPPKVTF 123

[Close]

BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: UB2E2_HUMAN (Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens GN=UBE2E2 PE=1 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 3.476e-11
Identity = 19/39 (48.72%), Postives = 31/39 (79.49%), Query Frame = 3

Query:   84 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP 200
            ++KRI KEL ++  DPP +CSAGP  ++++ W++TI+GP
Sbjct:   56 SAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGP 94

HSP 2 Score: 36.1946 bits (82), Expect = 3.476e-11
Identity = 15/25 (60.00%), Postives = 17/25 (68.00%), Query Frame = 2

Query:  215 YAGGVFLVTIHFPPDYPFQAPKGCF 289
            Y GGVF + I F PDYPF+ PK  F
Sbjct:   99 YEGGVFFLDITFSPDYPFKPPKVTF 123

[Close]

BLAST of CK739901 vs. ExPASy Swiss-Prot
Match: UB2E1_MOUSE (Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus GN=Ube2e1 PE=2 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 3.481e-11
Identity = 20/39 (51.28%), Postives = 31/39 (79.49%), Query Frame = 3

Query:   84 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP 200
            ++KRI KEL D+  DPP +CSAGP  ++++ W++TI+GP
Sbjct:   48 SAKRIQKELADITLDPPPNCSAGPKGDNIYEWRSTILGP 86

HSP 2 Score: 34.6538 bits (78), Expect = 3.481e-11
Identity = 14/25 (56.00%), Postives = 17/25 (68.00%), Query Frame = 2

Query:  215 YAGGVFLVTIHFPPDYPFQAPKGCF 289
            Y GGVF + I F P+YPF+ PK  F
Sbjct:   91 YEGGVFFLDITFTPEYPFKPPKVTF 115

The following BLAST results are available for this feature:

BLAST of CK739901 vs. ExPASy Swiss-Prot

BLAST of CK739901 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 41


Match Name	E-value	Identity	Description
UBC5_YEAST	6.428e-19	61.22	Ubiquitin-conjugating enzyme E2-16 kDa OS=Saccharo...	[more]
UBC4_CANAL	6.837e-18	70.00	Ubiquitin-conjugating enzyme E2 4 OS=Candida albic...	[more]
U2D2B_RAT	8.890e-18	80.00	Ubiquitin-conjugating enzyme E2 D2B OS=Rattus norv...	[more]
U2D2B_MOUSE	8.890e-18	80.00	Ubiquitin-conjugating enzyme E2 D2B OS=Mus musculu...	[more]
UBC12_ARATH	3.304e-17	64.71	Probable ubiquitin-conjugating enzyme E2 12 OS=Ara...	[more]
COP10_ARATH	7.283e-12	51.28	Constitutive photomorphogenesis protein 10 OS=Arab...	[more]
UBCD2_DROME	1.217e-11	48.72	Ubiquitin-conjugating enzyme E2-24 kDa OS=Drosophi...	[more]
UB2E2_MOUSE	3.476e-11	48.72	Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus...	[more]
UB2E2_HUMAN	3.476e-11	48.72	Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens...	[more]
UB2E1_MOUSE	3.481e-11	51.28	Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus...	[more]

Pages

Properties


Property Name	Value
Genbank description	USDA-FP_6293 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE61F04 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK739901 ID=CK739901; Name=CK739901; organism=Citrus sinensis; type=EST; length=290bp
CGCTCTCCGATCCTGTTCTCTGCCGCGCAAGGTCTTAAGGTTCGTTGTAG
TTTCAAATTTTAATAATCCCCTGAAGAGTCATGGCTTCAAAGCGTATTTT
GAAGGAACTCAAGGATTTGCAGAAGGATCCTCCCACGTCTTGCAGCGCTG
GCCCTGTGGCTGAAGACATGTTCCACTGGCAAGCAACAATTATGGGGCCC
TTCTGATAGCCCCATATGCAGGAGGTGTATTTTTAGTTACCATTCATTTT
CCTCCTGATTATCCCTTTCAAGCCCCCAAAGGTTGCTTTT