CK932981

Overview
NameCK932981
Unique NameCK932981
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length867
Libraries
Library NameType
Developing fruit juice sac at 38 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CK932981_ssr25 microsatellite CK932981_ssr25:25..36. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig7157 contig Csv1_Contig7157:95..957. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CK932981 vs. ExPASy Swiss-Prot
Match: ARF4_XENLA (ADP-ribosylation factor 4 OS=Xenopus laevis GN=arf4 PE=2 SV=2)

HSP 1 Score: 305.064 bits (780), Expect = 3.406e-82
Identity = 147/180 (81.67%), Postives = 162/180 (90.00%), Query Frame = 3 
Query:   90 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 629
            MGL+ + LFSRLF KK+MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNI FTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ EA +EL +ML EDELRDAVLLVFANKQDLPNAM  +E+TDKL L +LR R WY+Q+TCAT G GLYEGLDWLSN ++ +
Sbjct:    1 MGLTISSLFSRLFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERIQEAAEELQKMLQEDELRDAVLLVFANKQDLPNAMAISEMTDKLTLQTLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR 180
[Close]
BLAST of CK932981 vs. ExPASy Swiss-Prot
Match: ARF5_RAT (ADP-ribosylation factor 5 OS=Rattus norvegicus GN=Arf5 PE=1 SV=2)

HSP 1 Score: 303.523 bits (776), Expect = 9.911e-82
Identity = 145/180 (80.56%), Postives = 162/180 (90.00%), Query Frame = 3 
Query:   90 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 629
            MGL+ + LFSR+F KK+MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNI FTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+RV E+ DEL +ML EDELRDAVLLVFANKQD+PNAM  +E+TDKLGL  LR R WY+Q+TCAT G GLY+GLDWLS+ ++ +
Sbjct:    1 MGLTVSALFSRIFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVQESADELQKMLQEDELRDAVLLVFANKQDMPNAMPVSELTDKLGLQHLRSRTWYVQATCATQGTGLYDGLDWLSHELSKR 180
[Close]
BLAST of CK932981 vs. ExPASy Swiss-Prot
Match: ARF5_MOUSE (ADP-ribosylation factor 5 OS=Mus musculus GN=Arf5 PE=2 SV=2)

HSP 1 Score: 303.523 bits (776), Expect = 9.911e-82
Identity = 145/180 (80.56%), Postives = 162/180 (90.00%), Query Frame = 3 
Query:   90 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 629
            MGL+ + LFSR+F KK+MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNI FTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+RV E+ DEL +ML EDELRDAVLLVFANKQD+PNAM  +E+TDKLGL  LR R WY+Q+TCAT G GLY+GLDWLS+ ++ +
Sbjct:    1 MGLTVSALFSRIFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVQESADELQKMLQEDELRDAVLLVFANKQDMPNAMPVSELTDKLGLQHLRSRTWYVQATCATQGTGLYDGLDWLSHELSKR 180
[Close]
BLAST of CK932981 vs. ExPASy Swiss-Prot
Match: ARF5_HUMAN (ADP-ribosylation factor 5 OS=Homo sapiens GN=ARF5 PE=1 SV=2)

HSP 1 Score: 303.523 bits (776), Expect = 9.911e-82
Identity = 145/180 (80.56%), Postives = 162/180 (90.00%), Query Frame = 3 
Query:   90 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 629
            MGL+ + LFSR+F KK+MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNI FTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+RV E+ DEL +ML EDELRDAVLLVFANKQD+PNAM  +E+TDKLGL  LR R WY+Q+TCAT G GLY+GLDWLS+ ++ +
Sbjct:    1 MGLTVSALFSRIFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVQESADELQKMLQEDELRDAVLLVFANKQDMPNAMPVSELTDKLGLQHLRSRTWYVQATCATQGTGLYDGLDWLSHELSKR 180
[Close]
BLAST of CK932981 vs. ExPASy Swiss-Prot
Match: ARF4_PONAB (ADP-ribosylation factor 4 OS=Pongo abelii GN=ARF4 PE=2 SV=3)

HSP 1 Score: 301.982 bits (772), Expect = 2.884e-81
Identity = 145/180 (80.56%), Postives = 161/180 (89.44%), Query Frame = 3 
Query:   90 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 629
            MGL+ + LFSRLF KK+MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNI FTVWDVGGQD+IRPLW+HYFQNTQGLIFVVDSNDR+R+ E  DEL +ML  DELRDAVLL+FANKQDLPNAM  +E+TDKLGL SLR R WY+Q+TCAT G GLYEGLDWLSN ++ +
Sbjct:    1 MGLTISSLFSRLFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDRIRPLWKHYFQNTQGLIFVVDSNDRERIQEVADELQKMLLVDELRDAVLLLFANKQDLPNAMAISEMTDKLGLQSLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR 180
[Close]
BLAST of CK932981 vs. ExPASy Swiss-Prot
Match: ARF4_HUMAN (ADP-ribosylation factor 4 OS=Homo sapiens GN=ARF4 PE=1 SV=3)

HSP 1 Score: 301.982 bits (772), Expect = 2.884e-81
Identity = 145/180 (80.56%), Postives = 161/180 (89.44%), Query Frame = 3 
Query:   90 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 629
            MGL+ + LFSRLF KK+MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNI FTVWDVGGQD+IRPLW+HYFQNTQGLIFVVDSNDR+R+ E  DEL +ML  DELRDAVLL+FANKQDLPNAM  +E+TDKLGL SLR R WY+Q+TCAT G GLYEGLDWLSN ++ +
Sbjct:    1 MGLTISSLFSRLFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDRIRPLWKHYFQNTQGLIFVVDSNDRERIQEVADELQKMLLVDELRDAVLLLFANKQDLPNAMAISEMTDKLGLQSLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR 180
[Close]
BLAST of CK932981 vs. ExPASy Swiss-Prot
Match: ARF2_YEAST (ADP-ribosylation factor 2 OS=Saccharomyces cerevisiae GN=ARF2 PE=1 SV=3)

HSP 1 Score: 301.982 bits (772), Expect = 2.884e-81
Identity = 142/181 (78.45%), Postives = 165/181 (91.16%), Query Frame = 3 
Query:   90 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANKA 632
            MGL  +KLFS LF  KEMRILMVGLD AGKTT+LYKLKLGE++TTIPTIGFNVETV+YKNISFTVWDVGGQD+IR LWRHY++NT+G+IFV+DSNDR R+ EAR+ + RMLNEDELR+AV LVFANKQDLP AM+AAEIT+KLGLHS+R R W+IQSTCATSGEGLYEGL+WLSNN+ N++
Sbjct:    1 MGLYASKLFSNLFGNKEMRILMVGLDGAGKTTVLYKLKLGEVITTIPTIGFNVETVQYKNISFTVWDVGGQDRIRSLWRHYYRNTEGVIFVIDSNDRSRIGEAREVMQRMLNEDELRNAVWLVFANKQDLPEAMSAAEITEKLGLHSIRNRPWFIQSTCATSGEGLYEGLEWLSNNLKNQS 181
[Close]
BLAST of CK932981 vs. ExPASy Swiss-Prot
Match: ARF5_CHICK (ADP-ribosylation factor 5 OS=Gallus gallus GN=ARF5 PE=2 SV=2)

HSP 1 Score: 301.597 bits (771), Expect = 3.766e-81
Identity = 143/180 (79.44%), Postives = 163/180 (90.56%), Query Frame = 3 
Query:   90 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 629
            MGL+ + +FSR+F KK+MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNI FTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+RV E+ +EL +ML EDELRDAVLLVFANKQD+PNAM  +E+TDKLGL +LR R WY+Q+TCAT G GLY+GLDWLS+ ++ +
Sbjct:    1 MGLTVSAIFSRIFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVQESAEELQKMLQEDELRDAVLLVFANKQDMPNAMVVSELTDKLGLQALRSRTWYVQATCATQGTGLYDGLDWLSHELSKR 180
[Close]
BLAST of CK932981 vs. ExPASy Swiss-Prot
Match: ARF4_RAT (ADP-ribosylation factor 4 OS=Rattus norvegicus GN=Arf4 PE=2 SV=2)

HSP 1 Score: 299.671 bits (766), Expect = 1.431e-80
Identity = 145/180 (80.56%), Postives = 160/180 (88.89%), Query Frame = 3 
Query:   90 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 629
            MGL+ + LFSRLF KK+MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNI FTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ E    L +ML EDEL+DAVLL+FANKQDLPNAM  +E+TDKLGL SLR R WY+Q+TCAT G GLYEGLDWLSN ++ +
Sbjct:    1 MGLTISSLFSRLFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERIQEGAAVLQKMLLEDELQDAVLLLFANKQDLPNAMAISEMTDKLGLQSLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR 180
[Close]
BLAST of CK932981 vs. ExPASy Swiss-Prot
Match: ARF4_MOUSE (ADP-ribosylation factor 4 OS=Mus musculus GN=Arf4 PE=2 SV=2)

HSP 1 Score: 299.671 bits (766), Expect = 1.431e-80
Identity = 145/180 (80.56%), Postives = 160/180 (88.89%), Query Frame = 3 
Query:   90 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 629
            MGL+ + LFSRLF KK+MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNI FTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ E    L +ML EDEL+DAVLL+FANKQDLPNAM  +E+TDKLGL SLR R WY+Q+TCAT G GLYEGLDWLSN ++ +
Sbjct:    1 MGLTISSLFSRLFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERIQEGAAVLQKMLLEDELQDAVLLLFANKQDLPNAMAISEMTDKLGLQSLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR 180
The following BLAST results are available for this feature:
BLAST of CK932981 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 217
Match NameE-valueIdentityDescription
ARF4_XENLA3.406e-8281.67ADP-ribosylation factor 4 OS=Xenopus laevis GN=arf... [more]
ARF5_RAT9.911e-8280.56ADP-ribosylation factor 5 OS=Rattus norvegicus GN=... [more]
ARF5_MOUSE9.911e-8280.56ADP-ribosylation factor 5 OS=Mus musculus GN=Arf5 ... [more]
ARF5_HUMAN9.911e-8280.56ADP-ribosylation factor 5 OS=Homo sapiens GN=ARF5 ... [more]
ARF4_PONAB2.884e-8180.56ADP-ribosylation factor 4 OS=Pongo abelii GN=ARF4 ... [more]
ARF4_HUMAN2.884e-8180.56ADP-ribosylation factor 4 OS=Homo sapiens GN=ARF4 ... [more]
ARF2_YEAST2.884e-8178.45ADP-ribosylation factor 2 OS=Saccharomyces cerevis... [more]
ARF5_CHICK3.766e-8179.44ADP-ribosylation factor 5 OS=Gallus gallus GN=ARF5... [more]
ARF4_RAT1.431e-8080.56ADP-ribosylation factor 4 OS=Rattus norvegicus GN=... [more]
ARF4_MOUSE1.431e-8080.56ADP-ribosylation factor 4 OS=Mus musculus GN=Arf4 ... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004349_B12 Developing fruit juice sac at 38 DAFB Citrus sinensis cDNA clone JSJune0004_IIIF_B12 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK932981 ID=CK932981; Name=CK932981; organism=Citrus sinensis; type=EST; length=867bp
GGGAAGGACAGCTCGTATTTTTATAGAGAGAGAGAGGGGCAGTAGATCTG
CACCAAGGCAAAAACCGCTCTCTTCCTCAGTTGTGAAAAATGGGGCTGTC
TTTCACCAAGCTTTTCAGCAGGCTTTTTGCTAAGAAAGAGATGAGAATTC
TCATGGTAGGTCTCGATGCAGCTGGTAAGACTACTATCCTGTACAAGCTT
AAGCTCGGAGAGATCGTCACCACCATTCCTACCATTGGATTTAATGTAGA
GACCGTTGAGTATAAGAACATCAGCTTTACTGTCTGGGATGTCGGGGGTC
AAGACAAGATCCGACCTTTGTGGAGACATTATTTCCAAAACACCCAAGGA
CTCATCTTTGTGGTTGACAGTAATGATCGCGACCGTGTGGTAGAAGCTAG
GGATGAGTTGCACAGAATGTTGAATGAGGATGAATTGAGAGATGCTGTGC
TGCTTGTATTTGCTAACAAGCAAGATCTTCCAAATGCAATGAATGCTGCT
GAGATAACTGACAAGCTTGGCCTTCACTCTCTTCGCCAGCGCCACTGGTA
TATCCAGAGCACATGTGCCACTTCTGGTGAAGGGCTGTATGAGGGACTTG
ACTGGCTCTCAAACAACATTGCAAACAAGGCATAGACAACTAAATTTTGA
GTTTTGGGTTGCCGCAACTGCTGAAATACAGAGTTAACAACACATCTCAA
GTGTTCTTGCTTTCCTTCCTGTAATAGCTTCGTTGCCCGTTTTACTGTGT
TTGTTGTTTCCCTTGCACACGTTGAGATTTGTTTCTTGTAGCTTCAGAAT
NNNTCTTGAATGTTTATCTTTCCTCATGGTGTCCTCTCGAAAAAAAAAAA
AAAAAAAAAAAAAAAAA
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