CK934252

Overview
NameCK934252
Unique NameCK934252
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length725
Libraries
Library NameType
Developing fruit peel at 38 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig10723 contig Csv1_Contig10723:176..901. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CK934252 vs. ExPASy Swiss-Prot
Match: UBIQ_LEIMA (Ubiquitin OS=Leishmania major GN=UB-EP52 PE=3 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 2.177e-33
Identity = 71/76 (93.42%), Postives = 74/76 (97.37%), Query Frame = 1 
Query:   61 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 288
            MQIFVKTLTGKTI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76
[Close]
BLAST of CK934252 vs. ExPASy Swiss-Prot
Match: UBIQ_LEITA (Ubiquitin OS=Leishmania tarentolae GN=UB-EP52 PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 7.004e-32
Identity = 69/76 (90.79%), Postives = 72/76 (94.74%), Query Frame = 1 
Query:   61 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 288
            MQIFVKTLTG TI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFA KQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGTTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFADKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76
[Close]
BLAST of CK934252 vs. ExPASy Swiss-Prot
Match: UBIL_NPVOP (Ubiquitin-like protein OS=Orgyia pseudotsugata multicapsid polyhedrosis virus GN=V-UBI PE=3 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 2.754e-28
Identity = 61/80 (76.25%), Postives = 69/80 (86.25%), Query Frame = 1 
Query:   61 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGTMIK 300
            MQIFVKTLTGKTIT+E E  DT+  VK KI DKEG+P DQQRLI+AGKQLED +TLADYNIQKESTLH+VLRLRGG  ++
Sbjct:    1 MQIFVKTLTGKTITVETEPGDTVGQVKQKIADKEGVPVDQQRLIYAGKQLEDAQTLADYNIQKESTLHMVLRLRGGNGLR 80
[Close]
BLAST of CK934252 vs. ExPASy Swiss-Prot
Match: UBIL_NPVAC (Ubiquitin-like protein OS=Autographa californica nuclear polyhedrosis virus GN=V-UBI PE=3 SV=2)

HSP 1 Score: 122.479 bits (306), Expect = 2.332e-27
Identity = 58/76 (76.32%), Postives = 68/76 (89.47%), Query Frame = 1 
Query:   61 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 288
            MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRLIFAGKQLED +T+ADYNIQKESTLH+VLRLRGG
Sbjct:    1 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 76
[Close]
BLAST of CK934252 vs. ExPASy Swiss-Prot
Match: UBIQ_LUMTE (Ubiquitin (Fragment) OS=Lumbricus terrestris PE=1 SV=2)

HSP 1 Score: 120.553 bits (301), Expect = 8.860e-27
Identity = 60/63 (95.24%), Postives = 62/63 (98.41%), Query Frame = 1 
Query:   94 TITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLR 282
            TITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLR
Sbjct:    1 TITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR 63
[Close]
BLAST of CK934252 vs. ExPASy Swiss-Prot
Match: RUB1_DESAN (NEDD8-like protein RUB1 OS=Deschampsia antarctica GN=RUB1 PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 2.114e-20
Identity = 48/74 (64.86%), Postives = 59/74 (79.73%), Query Frame = 1 
Query:   67 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 288
            I VKTLTGK I +++E +DTID VK ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRVKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 94.3597 bits (233), Expect = 6.800e-19
Identity = 51/76 (67.11%), Postives = 52/76 (68.42%), Query Frame = 1 
Query:  289 TMIKVKTLTGKXXXXXXXXXXXXXXXXXXXXXXXGIPPVQQRLIYAGKQLADDKTARDYYIEGGSVLHLVLALRGG 516
            TMIKVKTLTGK                       GIPPVQQRLIYAGKQLADDKTA+DY IEGGSVLHLVLALRGG
Sbjct:    1 TMIKVKTLTGKEIEIDIEPTDTIDRVKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76
[Close]
BLAST of CK934252 vs. ExPASy Swiss-Prot
Match: NEDD8_DROME (NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 2.114e-20
Identity = 46/79 (58.23%), Postives = 60/79 (75.95%), Query Frame = 1 
Query:   61 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGTMI 297
            M I VKTLTGK I +++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG  I
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDSI 79

HSP 2 Score: 80.4925 bits (197), Expect = 1.016e-14
Identity = 42/75 (56.00%), Postives = 48/75 (64.00%), Query Frame = 1 
Query:  292 MIKVKTLTGKXXXXXXXXXXXXXXXXXXXXXXXGIPPVQQRLIYAGKQLADDKTARDYYIEGGSVLHLVLALRGG 516
            +IKVKTLTGK                       GIPP QQRLI++GKQ+ DDKTA DY ++GGSVLHLVLALRGG
Sbjct:    2 LIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGG 76
[Close]
BLAST of CK934252 vs. ExPASy Swiss-Prot
Match: RUB2_ORYSJ (NEDD8-like protein RUB2 OS=Oryza sativa subsp. japonica GN=RUB2 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 2.761e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 1 
Query:   67 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 288
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 94.3597 bits (233), Expect = 6.800e-19
Identity = 51/76 (67.11%), Postives = 52/76 (68.42%), Query Frame = 1 
Query:  289 TMIKVKTLTGKXXXXXXXXXXXXXXXXXXXXXXXGIPPVQQRLIYAGKQLADDKTARDYYIEGGSVLHLVLALRGG 516
            TMIKVKTLTGK                       GIPPVQQRLIYAGKQLADDKTA+DY IEGGSVLHLVLALRGG
Sbjct:    1 TMIKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76
[Close]
BLAST of CK934252 vs. ExPASy Swiss-Prot
Match: RUB1_ORYSJ (NEDD8-like protein RUB1 OS=Oryza sativa subsp. japonica GN=RUB1 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 2.761e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 1 
Query:   67 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 288
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 94.3597 bits (233), Expect = 6.800e-19
Identity = 51/76 (67.11%), Postives = 52/76 (68.42%), Query Frame = 1 
Query:  289 TMIKVKTLTGKXXXXXXXXXXXXXXXXXXXXXXXGIPPVQQRLIYAGKQLADDKTARDYYIEGGSVLHLVLALRGG 516
            TMIKVKTLTGK                       GIPPVQQRLIYAGKQLADDKTA+DY IEGGSVLHLVLALRGG
Sbjct:    1 TMIKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76
[Close]
BLAST of CK934252 vs. ExPASy Swiss-Prot
Match: RUB1_ARATH (NEDD8-like protein RUB1 OS=Arabidopsis thaliana GN=RUB1 PE=1 SV=2)

HSP 1 Score: 98.9821 bits (245), Expect = 2.761e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = 1 
Query:   67 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 288
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 94.3597 bits (233), Expect = 6.800e-19
Identity = 51/76 (67.11%), Postives = 52/76 (68.42%), Query Frame = 1 
Query:  289 TMIKVKTLTGKXXXXXXXXXXXXXXXXXXXXXXXGIPPVQQRLIYAGKQLADDKTARDYYIEGGSVLHLVLALRGG 516
            TMIKVKTLTGK                       GIPPVQQRLIYAGKQLADDKTA+DY IEGGSVLHLVLALRGG
Sbjct:    1 TMIKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76
The following BLAST results are available for this feature:
BLAST of CK934252 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 93
Match NameE-valueIdentityDescription
UBIQ_LEIMA2.177e-3393.42Ubiquitin OS=Leishmania major GN=UB-EP52 PE=3 SV=1[more]
UBIQ_LEITA7.004e-3290.79Ubiquitin OS=Leishmania tarentolae GN=UB-EP52 PE=3... [more]
UBIL_NPVOP2.754e-2876.25Ubiquitin-like protein OS=Orgyia pseudotsugata mul... [more]
UBIL_NPVAC2.332e-2776.32Ubiquitin-like protein OS=Autographa californica n... [more]
UBIQ_LUMTE8.860e-2795.24Ubiquitin (Fragment) OS=Lumbricus terrestris PE=1 ... [more]
RUB1_DESAN2.114e-2064.86NEDD8-like protein RUB1 OS=Deschampsia antarctica ... [more]
NEDD8_DROME2.114e-2058.23NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=... [more]
RUB2_ORYSJ2.761e-2063.51NEDD8-like protein RUB2 OS=Oryza sativa subsp. jap... [more]
RUB1_ORYSJ2.761e-2063.51NEDD8-like protein RUB1 OS=Oryza sativa subsp. jap... [more]
RUB1_ARATH2.761e-2063.51NEDD8-like protein RUB1 OS=Arabidopsis thaliana GN... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004261_E05 Developing fruit peel at 38 DAFB Citrus sinensis cDNA clone P38DAB10004_IVF_E05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK934252 ID=CK934252; Name=CK934252; organism=Citrus sinensis; type=EST; length=725bp
GGGGAAAGCTTTTTCGTTTTTCTTTTTCAAATCGAAGAGAAAGGAAAAAG
CAATCCTAATATGCAGATCTTTGTGAAGACTTTGACTGGGAAGACGATTA
CTCTCGAGGTTGAGAGCAGTGACACCATCGATAATGTTAAGGCTAAGATT
CAGGACAAGGAAGGCATTCCTCCCGATCAGCAGAGACTGATTTTTGCTGG
TAAACAATTGGAAGATGGCAGAACGCTTGCAGATTATAACATTCAAAAGG
AGTCGACGCTTCACTTGGTCCTGAGGCTCAGGGGAGGAACTATGATCAAG
GTGAAGACTCTCACAGGAAAGGAAATTGAAATTGATATTGAGCCAACTGA
TACCATCGAAAGGATCAAGGAAAGAGTGGAGGAAAAAGAAGGAATTCCTC
CAGTGCAGCAAAGGCTTATTTATGCTGGTAAGCAGCTTGCAGATGACAAG
ACAGCTCGCGACTACTACATTGAGGGTGGCTCCGTGCTTCATCTTGTTCT
TGCGCTGAGGGGTGGCAGTCTTTAAATGCATCTTTTCATATGAAGAGAAT
TCTACCTTATTCTGGCCTTATTTGGTTAAAGAAGTTGATTAACTTGTATG
AGTTTGAAAATAGAGAACATATTCTAATCAATTTCTCTGGGTTTAACATT
GATGTTTCTTCTTTTTTTGCTTAATATATTTTCCTATTATGCTTAACAAA
AAAAAAAAAAAAAAAAAAAAAAAAA
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