CK934737

Overview
NameCK934737
Unique NameCK934737
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length864
Libraries
Library NameType
Developing fruit peel at 38 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACTE_STRPU (Actin, cytoskeletal 3A OS=Strongylocentrotus purpuratus GN=CYIIIA PE=3 SV=1)

HSP 1 Score: 444.891 bits (1143), Expect = 2.738e-124
Identity = 217/241 (90.04%), Postives = 229/241 (95.02%), Query Frame = 3 
Query:  141 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            +D+  LV DNG+GMVKAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIV DSGDGVSHTVPIYEGYALPHAI+RLDLAGRDLTD LMKILTERGY FTTTAEREIVRD+KEKL YV LD+EQE++TA  SSS+EK+YELP
Sbjct:    4 DDVAALVVDNGSGMVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVFDSGDGVSHTVPIYEGYALPHAIIRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMQTAASSSSLEKSYELP 244
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACT1_HELTB (Actin CyI, cytoplasmic OS=Heliocidaris tuberculata PE=3 SV=1)

HSP 1 Score: 444.891 bits (1143), Expect = 2.738e-124
Identity = 218/241 (90.46%), Postives = 228/241 (94.61%), Query Frame = 3 
Query:  141 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            +D+  LV DNG+GMVKAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIV DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTD LMKILTERGY FTTTAEREIVRD+KEKL YV LD+EQE+ TA  SSS+EK+YELP
Sbjct:    4 DDVAALVIDNGSGMVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVFDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMSTAASSSSLEKSYELP 244
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACT1_HELER (Actin CyI, cytoplasmic OS=Heliocidaris erythrogramma PE=3 SV=1)

HSP 1 Score: 444.891 bits (1143), Expect = 2.738e-124
Identity = 218/241 (90.46%), Postives = 228/241 (94.61%), Query Frame = 3 
Query:  141 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            +D+  LV DNG+GMVKAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIV DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTD LMKILTERGY FTTTAEREIVRD+KEKL YV LD+EQE+ TA  SSS+EK+YELP
Sbjct:    4 DDVAALVIDNGSGMVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVFDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMSTAASSSSLEKSYELP 244
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACT_HYDAT (Actin, non-muscle 6.2 OS=Hydra attenuata PE=3 SV=1)

HSP 1 Score: 444.506 bits (1142), Expect = 3.577e-124
Identity = 219/245 (89.39%), Postives = 233/245 (95.10%), Query Frame = 3 
Query:  129 MADAEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            MAD +++  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAI+RLDLAGRDLTD LMKILTERGY FTTTAEREIVRD+KEKL+YV LD+EQE++TA  SSS+EK+YELP
Sbjct:    1 MAD-DEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAIIRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLSYVALDFEQEMQTAASSSSLEKSYELP 244
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACTC_HELTI (Actin, cytoplasmic OS=Helisoma trivolvis PE=3 SV=1)

HSP 1 Score: 444.506 bits (1142), Expect = 3.577e-124
Identity = 216/241 (89.63%), Postives = 229/241 (95.02%), Query Frame = 3 
Query:  141 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            ED+  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIV+DSGDGV+HTVPIYEGYALPHAI+RLDLAGRDLTD LMKILTERGY FTTTAEREIVRD+KEKL YV LD+EQE++TA  SSS+EK+YELP
Sbjct:    4 EDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVIDSGDGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMQTASTSSSLEKSYELP 244
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACTC_BRAFL (Actin, cytoplasmic OS=Branchiostoma floridae PE=2 SV=1)

HSP 1 Score: 444.506 bits (1142), Expect = 3.577e-124
Identity = 218/241 (90.46%), Postives = 228/241 (94.61%), Query Frame = 3 
Query:  141 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            +D+  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTD LMKILTERGY FTTTAEREIVRD+KEKL YV LD+EQE+ TA  SSS+EK+YELP
Sbjct:    3 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMSTAASSSSLEKSYELP 243
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACTC_BIOPF (Actin, cytoplasmic OS=Biomphalaria pfeifferi PE=3 SV=1)

HSP 1 Score: 444.506 bits (1142), Expect = 3.577e-124
Identity = 217/241 (90.04%), Postives = 228/241 (94.61%), Query Frame = 3 
Query:  141 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            ED+  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+RLDLAGRDLTD LMKILTERGY FTTTAEREIVRD+KEKL YV LD+EQE+ TA  SSS+EK+YELP
Sbjct:    4 EDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMHTAATSSSLEKSYELP 244
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACTC_BIOGL (Actin, cytoplasmic OS=Biomphalaria glabrata PE=2 SV=2)

HSP 1 Score: 444.506 bits (1142), Expect = 3.577e-124
Identity = 216/241 (89.63%), Postives = 229/241 (95.02%), Query Frame = 3 
Query:  141 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            ED+  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIV+DSGDGV+HTVPIYEGYALPHAI+RLDLAGRDLTD LMKILTERGY FTTTAEREIVRD+KEKL YV LD+EQE++TA  SSS+EK+YELP
Sbjct:    4 EDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMQTASTSSSLEKSYELP 244
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACTC_BIOAL (Actin, cytoplasmic OS=Biomphalaria alexandrina PE=3 SV=1)

HSP 1 Score: 444.506 bits (1142), Expect = 3.577e-124
Identity = 216/241 (89.63%), Postives = 229/241 (95.02%), Query Frame = 3 
Query:  141 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            ED+  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIV+DSGDGV+HTVPIYEGYALPHAI+RLDLAGRDLTD LMKILTERGY FTTTAEREIVRD+KEKL YV LD+EQE++TA  SSS+EK+YELP
Sbjct:    4 EDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMQTASTSSSLEKSYELP 244
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACTB_XENBO (Actin, cytoplasmic 1 OS=Xenopus borealis GN=actb PE=3 SV=1)

HSP 1 Score: 444.506 bits (1142), Expect = 3.577e-124
Identity = 220/245 (89.80%), Postives = 231/245 (94.29%), Query Frame = 3 
Query:  129 MADAEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            MAD +DI  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIV+DSGDGV+HTVPIYEGYALPHAILRLDLAGRDLTD LMKILTERGY FTTTAEREIVRD+KEKL YV LD+EQE+ TA  SSS+EK+YELP
Sbjct:    1 MAD-DDIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELP 244
The following BLAST results are available for this feature:
BLAST of CK934737 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 480
Match NameE-valueIdentityDescription
ACTE_STRPU2.738e-12490.04Actin, cytoskeletal 3A OS=Strongylocentrotus purpu... [more]
ACT1_HELTB2.738e-12490.46Actin CyI, cytoplasmic OS=Heliocidaris tuberculata... [more]
ACT1_HELER2.738e-12490.46Actin CyI, cytoplasmic OS=Heliocidaris erythrogram... [more]
ACT_HYDAT3.577e-12489.39Actin, non-muscle 6.2 OS=Hydra attenuata PE=3 SV=1[more]
ACTC_HELTI3.577e-12489.63Actin, cytoplasmic OS=Helisoma trivolvis PE=3 SV=1[more]
ACTC_BRAFL3.577e-12490.46Actin, cytoplasmic OS=Branchiostoma floridae PE=2 ... [more]
ACTC_BIOPF3.577e-12490.04Actin, cytoplasmic OS=Biomphalaria pfeifferi PE=3 ... [more]
ACTC_BIOGL3.577e-12489.63Actin, cytoplasmic OS=Biomphalaria glabrata PE=2 S... [more]
ACTC_BIOAL3.577e-12489.63Actin, cytoplasmic OS=Biomphalaria alexandrina PE=... [more]
ACTB_XENBO3.577e-12489.80Actin, cytoplasmic 1 OS=Xenopus borealis GN=actb P... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004227_D01 Developing fruit peel at 38 DAFB Citrus sinensis cDNA clone P38DAB10002_IIF_D01 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK934737 ID=CK934737; Name=CK934737; organism=Citrus sinensis; type=EST; length=864bp
GGGGACTACTACATTTTTAATCTCTCGGCAGTACCAAATACAGAGCGATA
GAGAGAATCGACACTCTCAGCTAGACTCCATCTTCGAATTTTTCTCTCGT
CGTCTCTTCAATCTAACTTTTATAAAAAATGGCCGATGCTGAGGATATTC
AACCCCTTGTTTGTGACAATGGAACTGGAATGGTGAAGGCTGGATTTGCT
GGAGATGATGCTCCAAGGGCAGTATTCCCTAGTATTGTTGGTAGGCCTCG
ACACACTGGAGTCATGGTTGGAATGGGGCAGAAGGATGCCTATGTTGGTG
ATGAGGCCCAATCGAAAAGAGGTATTCTTACTTTGAAATACCCAATTGAG
CACGGTATTGTAAGCAACTGGGATGATATGGAAAAGATCTGGCATCATAC
CTTCTACAACGAGCTTCGTGTTGCTCCTGAAGAGCACCCAGTGCTTCTCA
CTGAGGCACCTCTTAACCCCAAGGCCAACAGAGAGAAGATGACCCAAATT
ATGTTTGAAACTTTCAATGTGCCAGCCATGTACGTTGCCATCCAGGCTGT
TCTATCTCTTTATGCCAGTGGTCGTACAACTGGTATTGTGCTGGATTCTG
GTGATGGTGTGTCTCATACTGTACCAATTTATGAGGGATATGCCCTTCCT
CATGCCATTCTTCGTTTGGACTTGGCTGGTCGTGATCTCACTGATGCATT
GATGAAGATTCTTACTGAGAGAGGTTACATGTTTACCACCACAGCCGAAC
GGGAAATTGTCCGTGACATGAAGGAGAAGCTTGCTTATGTTGTCCTAGAC
TATGAGCAAGAACTTGAGACTGCCNNNNGCAGCTCATCTGTTGAGAAGAA
CTATGAGCTGCCTG
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