CK934737

Overview
NameCK934737
Unique NameCK934737
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length864
Libraries
Library NameType
Developing fruit peel at 38 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACTB_STRPU (Actin, cytoskeletal 1B OS=Strongylocentrotus purpuratus GN=CYIB PE=3 SV=1)

HSP 1 Score: 444.506 bits (1142), Expect = 3.577e-124
Identity = 217/241 (90.04%), Postives = 229/241 (95.02%), Query Frame = 3 
Query:  141 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            +D+  LV DNG+GMVKAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYP+EHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIV DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTD LMKILTERGY FTTTAEREIVRD+KEKL YV LD+EQE++TA  SSS+EK+YELP
Sbjct:    4 DDVAALVIDNGSGMVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPMEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVFDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMQTAASSSSLEKSYELP 244
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACTA_STRPU (Actin, cytoskeletal 1A OS=Strongylocentrotus purpuratus GN=CYIA PE=3 SV=1)

HSP 1 Score: 444.506 bits (1142), Expect = 3.577e-124
Identity = 217/241 (90.04%), Postives = 229/241 (95.02%), Query Frame = 3 
Query:  141 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            +D+  LV DNG+GMVKAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYP+EHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIV DSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTD LMKILTERGY FTTTAEREIVRD+KEKL YV LD+EQE++TA  SSS+EK+YELP
Sbjct:    4 DDVAALVIDNGSGMVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPMEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVFDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMQTAASSSSLEKSYELP 244
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACT5C_ANOGA (Actin-5C OS=Anopheles gambiae GN=Act5C PE=2 SV=1)

HSP 1 Score: 444.506 bits (1142), Expect = 3.577e-124
Identity = 218/241 (90.46%), Postives = 228/241 (94.61%), Query Frame = 3 
Query:  141 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            E++  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTD LMKILTERGY FTTTAEREIVRD+KEKL YV LD+EQE+ TA  SSS+EK+YELP
Sbjct:    4 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELP 244
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACT4_BOMMO (Actin, cytoplasmic A4 OS=Bombyx mori GN=A4 PE=2 SV=1)

HSP 1 Score: 444.506 bits (1142), Expect = 3.577e-124
Identity = 218/241 (90.46%), Postives = 228/241 (94.61%), Query Frame = 3 
Query:  141 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            E++  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTD LMKILTERGY FTTTAEREIVRD+KEKL YV LD+EQE+ TA  SSS+EK+YELP
Sbjct:    4 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELP 244
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACT3_BOMMO (Actin, cytoplasmic A3 OS=Bombyx mori PE=3 SV=3)

HSP 1 Score: 444.506 bits (1142), Expect = 3.577e-124
Identity = 218/241 (90.46%), Postives = 228/241 (94.61%), Query Frame = 3 
Query:  141 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            E++  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTD LMKILTERGY FTTTAEREIVRD+KEKL YV LD+EQE+ TA  SSS+EK+YELP
Sbjct:    4 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELP 244
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACT3B_HELAM (Actin-A3b, cytoplasmic OS=Helicoverpa armigera GN=actA3b PE=2 SV=1)

HSP 1 Score: 444.506 bits (1142), Expect = 3.577e-124
Identity = 218/241 (90.46%), Postives = 228/241 (94.61%), Query Frame = 3 
Query:  141 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            E++  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTD LMKILTERGY FTTTAEREIVRD+KEKL YV LD+EQE+ TA  SSS+EK+YELP
Sbjct:    4 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELP 244
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACT3A_HELAM (Actin, cytoplasmic A3a OS=Helicoverpa armigera GN=actA3a PE=2 SV=1)

HSP 1 Score: 444.506 bits (1142), Expect = 3.577e-124
Identity = 218/241 (90.46%), Postives = 228/241 (94.61%), Query Frame = 3 
Query:  141 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            E++  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTD LMKILTERGY FTTTAEREIVRD+KEKL YV LD+EQE+ TA  SSS+EK+YELP
Sbjct:    4 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELP 244
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACT2_MOLOC (Actin, muscle-type OS=Molgula oculata PE=3 SV=1)

HSP 1 Score: 444.506 bits (1142), Expect = 3.577e-124
Identity = 219/246 (89.02%), Postives = 231/246 (93.90%), Query Frame = 3 
Query:  129 MADAEDIQP-LVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            M D E+ Q  LVCDNG+G+VKAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGI++NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSH VPIYEGYALPHAI+RLDLAGRDLTD LMKILTERGY F TTAEREIVRD+KEKL YV LD+EQE+ TA  SSS+EK+YELP
Sbjct:    1 MEDEEEEQTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELP 246
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACT2_LUMTE (Actin-2 OS=Lumbricus terrestris GN=ACT2 PE=2 SV=1)

HSP 1 Score: 444.506 bits (1142), Expect = 3.577e-124
Identity = 218/241 (90.46%), Postives = 228/241 (94.61%), Query Frame = 3 
Query:  141 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            ED+  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAILRLDLAGRDLTD LMKILTERGY FTTTAEREIVRD+KEKL YV LD+EQE+ TA  SSS+EK+YELP
Sbjct:    4 EDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMNTASASSSLEKSYELP 244
[Close]
BLAST of CK934737 vs. ExPASy Swiss-Prot
Match: ACT2_DROME (Actin-42A OS=Drosophila melanogaster GN=Act42A PE=1 SV=3)

HSP 1 Score: 444.506 bits (1142), Expect = 3.577e-124
Identity = 218/241 (90.46%), Postives = 228/241 (94.61%), Query Frame = 3 
Query:  141 EDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDALMKILTERGYMFTTTAEREIVRDMKEKLAYVVLDYEQELETAXXSSSVEKNYELP 863
            E++  LV DNG+GM KAGFAGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTD LMKILTERGY FTTTAEREIVRD+KEKL YV LD+EQE+ TA  SSS+EK+YELP
Sbjct:    4 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELP 244
The following BLAST results are available for this feature:
BLAST of CK934737 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 480
Match NameE-valueIdentityDescription
ACTB_STRPU3.577e-12490.04Actin, cytoskeletal 1B OS=Strongylocentrotus purpu... [more]
ACTA_STRPU3.577e-12490.04Actin, cytoskeletal 1A OS=Strongylocentrotus purpu... [more]
ACT5C_ANOGA3.577e-12490.46Actin-5C OS=Anopheles gambiae GN=Act5C PE=2 SV=1[more]
ACT4_BOMMO3.577e-12490.46Actin, cytoplasmic A4 OS=Bombyx mori GN=A4 PE=2 SV... [more]
ACT3_BOMMO3.577e-12490.46Actin, cytoplasmic A3 OS=Bombyx mori PE=3 SV=3[more]
ACT3B_HELAM3.577e-12490.46Actin-A3b, cytoplasmic OS=Helicoverpa armigera GN=... [more]
ACT3A_HELAM3.577e-12490.46Actin, cytoplasmic A3a OS=Helicoverpa armigera GN=... [more]
ACT2_MOLOC3.577e-12489.02Actin, muscle-type OS=Molgula oculata PE=3 SV=1[more]
ACT2_LUMTE3.577e-12490.46Actin-2 OS=Lumbricus terrestris GN=ACT2 PE=2 SV=1[more]
ACT2_DROME3.577e-12490.46Actin-42A OS=Drosophila melanogaster GN=Act42A PE=... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004227_D01 Developing fruit peel at 38 DAFB Citrus sinensis cDNA clone P38DAB10002_IIF_D01 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK934737 ID=CK934737; Name=CK934737; organism=Citrus sinensis; type=EST; length=864bp
GGGGACTACTACATTTTTAATCTCTCGGCAGTACCAAATACAGAGCGATA
GAGAGAATCGACACTCTCAGCTAGACTCCATCTTCGAATTTTTCTCTCGT
CGTCTCTTCAATCTAACTTTTATAAAAAATGGCCGATGCTGAGGATATTC
AACCCCTTGTTTGTGACAATGGAACTGGAATGGTGAAGGCTGGATTTGCT
GGAGATGATGCTCCAAGGGCAGTATTCCCTAGTATTGTTGGTAGGCCTCG
ACACACTGGAGTCATGGTTGGAATGGGGCAGAAGGATGCCTATGTTGGTG
ATGAGGCCCAATCGAAAAGAGGTATTCTTACTTTGAAATACCCAATTGAG
CACGGTATTGTAAGCAACTGGGATGATATGGAAAAGATCTGGCATCATAC
CTTCTACAACGAGCTTCGTGTTGCTCCTGAAGAGCACCCAGTGCTTCTCA
CTGAGGCACCTCTTAACCCCAAGGCCAACAGAGAGAAGATGACCCAAATT
ATGTTTGAAACTTTCAATGTGCCAGCCATGTACGTTGCCATCCAGGCTGT
TCTATCTCTTTATGCCAGTGGTCGTACAACTGGTATTGTGCTGGATTCTG
GTGATGGTGTGTCTCATACTGTACCAATTTATGAGGGATATGCCCTTCCT
CATGCCATTCTTCGTTTGGACTTGGCTGGTCGTGATCTCACTGATGCATT
GATGAAGATTCTTACTGAGAGAGGTTACATGTTTACCACCACAGCCGAAC
GGGAAATTGTCCGTGACATGAAGGAGAAGCTTGCTTATGTTGTCCTAGAC
TATGAGCAAGAACTTGAGACTGCCNNNNGCAGCTCATCTGTTGAGAAGAA
CTATGAGCTGCCTG
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