EY736438

Overview
NameEY736438
Unique NameEY736438
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length749
Libraries
Library NameType
Sweet orange fruit, development stadium (6 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLNA4_MAIZE (Glutamine synthetase root isozyme 4 OS=Zea mays GN=GLN5 PE=2 SV=1)

HSP 1 Score: 250.751 bits (639), Expect = 3.457e-67
Identity = 119/154 (77.27%), Postives = 133/154 (86.36%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            T+KIIAEYIWIGGSGMD+RSKARTLPGPV+DPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCD YTPAGEPIPTNKR++AA+IFS  +V  EEPWYGIEQE    +   N  LG P  G+PG QGPYY  G+G ++++G
Sbjct:   16 TEKIIAEYIWIGGSGMDLRSKARTLPGPVTDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDCYTPAGEPIPTNKRYSAAKIFSSPEVAAEEPWYGIEQEYTLLQKDTNWPLGWPIGGFPGPQGPYY-CGIGAEKSFG 168

HSP 2 Score: 26.1794 bits (56), Expect = 3.457e-67
Identity = 13/23 (56.52%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ D HYKA L AG+
Sbjct:  162 GAEKSFGRDIVDAHYKACLYAGI 184
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLNA2_PHAVU (Glutamine synthetase PR-2 OS=Phaseolus vulgaris PE=2 SV=1)

HSP 1 Score: 251.136 bits (640), Expect = 4.508e-67
Identity = 119/154 (77.27%), Postives = 132/154 (85.71%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            T+KIIAEYIW+GGSGMD+RSKARTLPGPV DP+KLPKWNYDGSST QAPG+DSEVILYPQAIFKDPFRRGNNILV+CD YTPAGEP+PTNKR+ AA+IFSH DVV E PWYGIEQE    +  +N  LG P  GYPG QGPYY  GVG D+A+G
Sbjct:   16 TEKIIAEYIWVGGSGMDLRSKARTLPGPVDDPAKLPKWNYDGSSTDQAPGDDSEVILYPQAIFKDPFRRGNNILVICDVYTPAGEPLPTNKRYDAAKIFSHPDVVAEVPWYGIEQEYTLLQKDVNWPLGWPLGGYPGPQGPYY-CGVGADKAYG 168

HSP 2 Score: 25.409 bits (54), Expect = 4.508e-67
Identity = 12/23 (52.17%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ D HYKA + AG+
Sbjct:  162 GADKAYGRDIVDAHYKACVYAGI 184
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLN11_ORYSJ (Glutamine synthetase cytosolic isozyme 1-1 OS=Oryza sativa subsp. japonica GN=GLN1-1 PE=1 SV=1)

HSP 1 Score: 250.366 bits (638), Expect = 4.508e-67
Identity = 119/154 (77.27%), Postives = 132/154 (85.71%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            T+KIIAEYIWIGGSGMD+RSKARTL GPV+DPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFR+GNNILVMCD YTPAGEPIPTNKRH AA+IFS  +V  EEPWYGIEQE    +  +N  LG P  G+PG QGPYY  G+G D+++G
Sbjct:   16 TEKIIAEYIWIGGSGMDLRSKARTLSGPVTDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRKGNNILVMCDCYTPAGEPIPTNKRHNAAKIFSSPEVASEEPWYGIEQEYTLLQKDINWPLGWPVGGFPGPQGPYY-CGIGADKSFG 168

HSP 2 Score: 26.1794 bits (56), Expect = 4.508e-67
Identity = 13/23 (56.52%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ D HYKA L AG+
Sbjct:  162 GADKSFGRDIVDSHYKACLYAGI 184
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLNA1_VITVI (Glutamine synthetase cytosolic isozyme 1 OS=Vitis vinifera GN=GS1-1 PE=2 SV=1)

HSP 1 Score: 249.21 bits (635), Expect = 5.879e-67
Identity = 117/154 (75.97%), Postives = 129/154 (83.77%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            T+K+I EYIW+GGSGMD+RSKARTL GPVSDP+KLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCD YTPAGEPIPTNKR  AA+IFSH DV  E PWYGIEQE    +  +   +G P  G+PG QGPYY  G+G D+AWG
Sbjct:   16 TEKVIVEYIWVGGSGMDLRSKARTLSGPVSDPAKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDTYTPAGEPIPTNKRCNAAKIFSHPDVAAEVPWYGIEQEYTLLQKEVKWPIGWPVGGFPGPQGPYY-CGIGADKAWG 168

HSP 2 Score: 26.9498 bits (58), Expect = 5.879e-67
Identity = 13/23 (56.52%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ D HYKA L AG+
Sbjct:  162 GADKAWGRDIVDAHYKACLYAGI 184
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLNA3_PEA (Glutamine synthetase root isozyme A OS=Pisum sativum GN=GS3A PE=2 SV=1)

HSP 1 Score: 249.21 bits (635), Expect = 7.666e-67
Identity = 117/154 (75.97%), Postives = 132/154 (85.71%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            T+KIIAEYIW+GGSG+D+RSKARTLPGPVSDP+KLPKWNYDGSST QAPG+DSEVILYPQAIFKDPFRRGNNILV+CD YTPAGEP+PTNKR+ AA+IFSH DV  E PWYGIEQE    +  +N  LG P  GYPG QGPYY  G+G D+A+G
Sbjct:   16 TEKIIAEYIWVGGSGIDIRSKARTLPGPVSDPAKLPKWNYDGSSTNQAPGKDSEVILYPQAIFKDPFRRGNNILVICDVYTPAGEPLPTNKRYNAAKIFSHPDVAAEVPWYGIEQEYTLLQKDINWPLGWPIGGYPGKQGPYY-CGIGADKAYG 168

HSP 2 Score: 26.5646 bits (57), Expect = 7.666e-67
Identity = 13/23 (56.52%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ D HYKA L AG+
Sbjct:  162 GADKAYGRDIVDAHYKACLFAGI 184
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLN14_ARATH (Glutamine synthetase cytosolic isozyme 1-4 OS=Arabidopsis thaliana GN=GLN1-4 PE=1 SV=1)

HSP 1 Score: 249.21 bits (635), Expect = 7.666e-67
Identity = 119/154 (77.27%), Postives = 132/154 (85.71%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            TD+IIAEYIWIGGSG+DMRSKARTLPGPV+DPS+LPKWNYDGSSTGQAPG+DSEVI+YPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAA+IF    VV EE WYGIEQE    +  +   +G P  G+PG QGPYY  GVG D+A+G
Sbjct:   16 TDQIIAEYIWIGGSGLDMRSKARTLPGPVTDPSQLPKWNYDGSSTGQAPGDDSEVIIYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAAKIFEDPSVVAEETWYGIEQEYTLLQKDIKWPVGWPVGGFPGPQGPYY-CGVGADKAFG 168

HSP 2 Score: 26.5646 bits (57), Expect = 7.666e-67
Identity = 13/23 (56.52%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ D HYKA L AG+
Sbjct:  162 GADKAFGRDIVDSHYKACLYAGI 184
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLNA_LUPLU (Glutamine synthetase nodule isozyme OS=Lupinus luteus PE=3 SV=1)

HSP 1 Score: 249.21 bits (635), Expect = 7.667e-67
Identity = 117/154 (75.97%), Postives = 134/154 (87.01%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            T+KIIAEYIW+GGSG+D+RSKARTL GPV+DPSKLPKWNYDGSSTGQAPG+DSEVIL+PQAIFKDPFRRGNNILVMCD YTPAGEPIPTNKRHAAA+IFSH DVV EEPW+GIEQE    +  ++  +G P  G+PG QGPYY  G G ++A+G
Sbjct:   16 TEKIIAEYIWVGGSGVDLRSKARTLSGPVNDPSKLPKWNYDGSSTGQAPGKDSEVILWPQAIFKDPFRRGNNILVMCDTYTPAGEPIPTNKRHAAAKIFSHPDVVAEEPWFGIEQEYTLLQKDIHWPIGWPLGGFPGPQGPYY-CGTGAEKAFG 168

HSP 2 Score: 26.5646 bits (57), Expect = 7.667e-67
Identity = 13/23 (56.52%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ D HYKA L AG+
Sbjct:  162 GAEKAFGRDIVDSHYKACLYAGI 184
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLNA4_PEA (Glutamine synthetase root isozyme B OS=Pisum sativum GN=GS3B PE=3 SV=1)

HSP 1 Score: 248.825 bits (634), Expect = 9.996e-67
Identity = 117/154 (75.97%), Postives = 132/154 (85.71%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            T+KIIAEYIW+GGSG+D+RSKARTLPGPVSDP+KLPKWNYDGSST QAPG+DSEVILYPQAIFKDPFRRGNNILV+CD YTPAGEP+PTNKR+ AA+IFSH DV  E PWYGIEQE    +  +N  LG P  GYPG QGPYY  G+G D+A+G
Sbjct:   16 TEKIIAEYIWVGGSGIDIRSKARTLPGPVSDPAKLPKWNYDGSSTDQAPGKDSEVILYPQAIFKDPFRRGNNILVICDVYTPAGEPLPTNKRYNAAKIFSHPDVAAEVPWYGIEQEYTLLQKDINWPLGWPIGGYPGKQGPYY-CGIGADKAYG 168

HSP 2 Score: 26.5646 bits (57), Expect = 9.996e-67
Identity = 13/23 (56.52%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ D HYKA L AG+
Sbjct:  162 GADKAYGRDIVDAHYKACLFAGI 184
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLNA3_MEDSA (Glutamine synthetase nodule isozyme OS=Medicago sativa GN=GS1 PE=2 SV=1)

HSP 1 Score: 248.054 bits (632), Expect = 1.700e-66
Identity = 116/154 (75.32%), Postives = 131/154 (85.06%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            ++KIIAEYIW+GGSGMD+RSKARTLPGPVSDP+KLPKWNYDGSST QAPG+DSEVILYPQAIFKDPFR+GNNILV+CD YTPAGEP+PTNKRH AA+IFSH DV  E PWYGIEQE    +   N  LG P  G+PG QGPYY  G+G D+A+G
Sbjct:   16 SEKIIAEYIWVGGSGMDLRSKARTLPGPVSDPAKLPKWNYDGSSTNQAPGQDSEVILYPQAIFKDPFRQGNNILVICDVYTPAGEPLPTNKRHNAAKIFSHPDVAAEVPWYGIEQEYTLLQKDTNWPLGWPIGGFPGPQGPYY-CGIGADKAYG 168

HSP 2 Score: 26.5646 bits (57), Expect = 1.700e-66
Identity = 13/23 (56.52%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ D HYKA L AG+
Sbjct:  162 GADKAYGRDIVDAHYKACLYAGI 184
[Close]
BLAST of EY736438 vs. ExPASy Swiss-Prot
Match: GLNA2_SOYBN (Glutamine synthetase cytosolic isozyme 2 OS=Glycine max PE=2 SV=1)

HSP 1 Score: 246.899 bits (629), Expect = 4.916e-66
Identity = 117/154 (75.97%), Postives = 130/154 (84.42%), Query Frame = 2 
Query:  128 TDKIIAEYIWIGGSGMDMRSKARTLPGPVSDPSKLPKWNYDGSSTGQAPGEDSEVILYPQAIFKDPFRRGNNILVMCDAYTPAGEPIPTNKRHAAARIFSHSDVVGEEPWYGIEQE*PYCRTMLNGLLGGPSVGYPGAQGPYYWWGVGPDQAWG 589
            TDK+IAEYIW+GGSGMDMRSKARTL GPV DPSKLPKWNYDGSSTGQAPG+DSEVILYPQAIFKDPFRRG+NILVMCDAYTPAGEPIPTNKR+ AA+IF H DV  EEPWYG+EQE    +  +   LG P  G+PG QGPYY  G G ++A+G
Sbjct:   16 TDKVIAEYIWVGGSGMDMRSKARTLSGPVKDPSKLPKWNYDGSSTGQAPGQDSEVILYPQAIFKDPFRRGSNILVMCDAYTPAGEPIPTNKRNNAAKIFGHPDVAAEEPWYGLEQEYTLLQKDVQWPLGWPLGGFPGPQGPYY-CGTGANKAFG 168

HSP 2 Score: 26.1794 bits (56), Expect = 4.916e-66
Identity = 12/23 (52.17%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  570 GRTKLGGRDMGDPHYKAGL*AGM 638
            G  K  GRD+ D HYKA + AG+
Sbjct:  162 GANKAFGRDIVDSHYKACIYAGI 184
The following BLAST results are available for this feature:
BLAST of EY736438 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 87
Match NameE-valueIdentityDescription
GLNA4_MAIZE3.457e-6777.27Glutamine synthetase root isozyme 4 OS=Zea mays GN... [more]
GLNA2_PHAVU4.508e-6777.27Glutamine synthetase PR-2 OS=Phaseolus vulgaris PE... [more]
GLN11_ORYSJ4.508e-6777.27Glutamine synthetase cytosolic isozyme 1-1 OS=Oryz... [more]
GLNA1_VITVI5.879e-6775.97Glutamine synthetase cytosolic isozyme 1 OS=Vitis ... [more]
GLNA3_PEA7.666e-6775.97Glutamine synthetase root isozyme A OS=Pisum sativ... [more]
GLN14_ARATH7.666e-6777.27Glutamine synthetase cytosolic isozyme 1-4 OS=Arab... [more]
GLNA_LUPLU7.667e-6775.97Glutamine synthetase nodule isozyme OS=Lupinus lut... [more]
GLNA4_PEA9.996e-6775.97Glutamine synthetase root isozyme B OS=Pisum sativ... [more]
GLNA3_MEDSA1.700e-6675.32Glutamine synthetase nodule isozyme OS=Medicago sa... [more]
GLNA2_SOYBN4.916e-6675.97Glutamine synthetase cytosolic isozyme 2 OS=Glycin... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-705-016-D01-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY736438 ID=EY736438; Name=EY736438; organism=Citrus sinensis; type=EST; length=749bp
CCACGCGTCCGCATCCCTTCAATCACAGTAATCTCATTACCAGTTTCTTT
GTTGTGTTAAGAGACTAAAGAGTCTTCGAAACATGTCTCTGCTCTCAGAT
CTCCTTAACCTTAACCTCTCAGAATCCACTGACAAGATCATCGCTGAGTA
CATATGGATCGGTGGATCTGGCATGGACATGAGGAGCAAAGCAAGGACTC
TTCCCGGTCCCGTTAGTGATCCTTCCAAGCTGCCTAAGTGGAATTATGAT
GGTTCCAGCACAGGCCAAGCACCTGGAGAAGATAGTGAAGTGATCCTCTA
TCCTCAAGCTATTTTCAAGGATCCATTCAGGAGAGGCAACAATATTCTTG
TTATGTGTGATGCTTACACTCCTGCGGGGGAACCAATTCCAACAAACAAA
AGACATGCAGCTGCCAGGATTTTCAGCCATTCTGACGTTGTGGGTGAAGA
ACCATGGTATGGTATTGAGCAGGAATAACCTTACTGCAGAACGATGTTAA
ATGGCCTCTTGGGTGGCCCATCCGTGGGTTATCCTGGGGCTCAGGGACCA
TACTACTGGTGGGGTGTGGGGCCGGACCAAGCTTGGGGGGCGCGACATGG
GGGACCCCCATTACAAGGCCGGTCTATAGGCGGGGATGAGCGTTGGGGGG
GGGGAGGGGGAAAGTTGGCCGGGGCCAGGGGGAGTTCCAAGTGCGCTCCC
CGCGCGGGGATAGTCCGCGGAGGAGTGGGTGGGGCGGGTGCGGGCGGCC
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