EY736404

Overview
NameEY736404
Unique NameEY736404
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length700
Libraries
Library NameType
Sweet orange fruit, development stadium (6 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY736404 vs. ExPASy Swiss-Prot
Match: 6PGD_HAEIN (6-phosphogluconate dehydrogenase, decarboxylating OS=Haemophilus influenzae GN=gnd PE=3 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 1.127e-21
Identity = 49/112 (43.75%), Postives = 65/112 (58.04%), Query Frame = 2 
Query:  110 IGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKKEGDLPLFGFLDPESFVNSIXKPRVXIMXC*AGAPCDEXHXTLSAYMEKGDXIIDGGYEWYENTERGEKA 445
            IG+ GLAVMGQNL LN+ + GF +  YNRTTSKVDE ++ A K  +  + G    E     + KPR  ++   AG   D+    L  ++E+GD IIDGG   Y +T R  KA
Sbjct:    7 IGVIGLAVMGQNLILNMNDHGFKVVAYNRTTSKVDEFLQGAAKGTN--IIGAYSLEDLAAKLEKPRKVMLMVRAGDVVDQFIEALLPHLEEGDIIIDGGNSNYPDTNRRVKA 116

HSP 2 Score: 40.4318 bits (93), Expect = 1.127e-21
Identity = 24/79 (30.38%), Postives = 41/79 (51.90%), Query Frame = 3 
Query:  456 GFALPGMGISSGEESAKNGSSLMPGRSFQGYNLLKTFS*RRGFKS*YTAP-CELQLQRGSGYFGKIILLGLKSGYSPLL 689
            G    G G+S GEE A++G S+MPG + + +  +K        K+    P C+     G+G+F K++  G++ G   L+
Sbjct:  121 GIRFIGSGVSGGEEGARHGPSIMPGGNQEAWQYVKPIFQAISAKTEQGEPCCDWVGGEGAGHFVKMVHNGIEYGDMQLI 199
[Close]
BLAST of EY736404 vs. ExPASy Swiss-Prot
Match: 6PGD1_YEAST (6-phosphogluconate dehydrogenase, decarboxylating 1 OS=Saccharomyces cerevisiae GN=GND1 PE=1 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.466e-21
Identity = 45/107 (42.06%), Postives = 61/107 (57.01%), Query Frame = 2 
Query:  113 GLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKKEGDLPLFGFLDPESFVNSIXKPRVXIMXC*AGAPCDEXHXTLSAYMEKGDXIIDGGYEWYENTER 433
            GL GLAVMGQNL LN A+ GF +  YNRT SKVD  +  A +     + G    E F++ + +PR  ++   AGAP D     +   +EKGD IIDGG   + ++ R
Sbjct:    6 GLIGLAVMGQNLILNAADHGFTVCAYNRTQSKVDHFL--ANEAKGKSIIGATSIEDFISKLKRPRKVMLLVKAGAPVDALINQIVPLLEKGDIIIDGGNSHFPDSNR 110

HSP 2 Score: 42.3578 bits (98), Expect = 1.466e-21
Identity = 26/78 (33.33%), Postives = 39/78 (50.00%), Query Frame = 3 
Query:  456 GFALPGMGISSGEESAKNGSSLMPGRSFQGYNLLKTFS*RRGFKS*YTAPCELQLQRGSGYFGKIILLGLKSGYSPLL 689
            G    G G+S GEE A+ G SLMPG S + +  +K        KS     CE     G+G++ K++  G++ G   L+
Sbjct:  119 GILFVGSGVSGGEEGARYGPSLMPGGSEEAWPHIKNIFQSISAKSDGEPCCEWVGPAGAGHYVKMVHNGIEYGDMQLI 196
[Close]
BLAST of EY736404 vs. ExPASy Swiss-Prot
Match: 6PGD_BUCAI (6-phosphogluconate dehydrogenase, decarboxylating OS=Buchnera aphidicola subsp. Acyrthosiphon pisum GN=gnd PE=3 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 4.201e-21
Identity = 45/113 (39.82%), Postives = 69/113 (61.06%), Query Frame = 2 
Query:  107 RIGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKKEGDLPLFGFLDPESFVNSIXKPRVXIMXC*AGAPCDEXHXTLSAYMEKGDXIIDGGYEWYENT-ERGEK 442
            +IG+ G+AVMG+NLALNI  K + +S++NRT S  +E   + KK+  +P F   D   F++S+ KPR  ++   +G   DE    +  Y+EK D +ID G  +Y++T  R EK
Sbjct:    5 QIGVVGMAVMGRNLALNIESKNYSVSIFNRTRSVTEEVFNQNKKKNIVPYFSIKD---FIDSLLKPRCILLMVQSGKATDETIKMILPYLEKEDILIDAGNTFYKDTIRRNEK 114

HSP 2 Score: 36.965 bits (84), Expect = 4.201e-21
Identity = 24/74 (32.43%), Postives = 38/74 (51.35%), Query Frame = 3 
Query:  471 GMGISSGEESAKNGSSLMPGRSFQGYNLLKTFS*RRGFKS*YTAPCELQL-QRGSGYFGKIILLGLKSGYSPLL 689
            GMG+S GE  A NG S+MPG   + Y L+     +   K     PC   +   G+G++ K++  G++ G   L+
Sbjct:  124 GMGVSGGELGALNGPSIMPGGQKEAYKLVLPMLEKISAKF-KGEPCVSYIGPNGAGHYVKMVHNGIEYGDMQLI 196
[Close]
BLAST of EY736404 vs. ExPASy Swiss-Prot
Match: 6PGD_STAAW (6-phosphogluconate dehydrogenase, decarboxylating OS=Staphylococcus aureus (strain MW2) GN=gnd PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.203e-20
Identity = 47/113 (41.59%), Postives = 68/113 (60.18%), Query Frame = 2 
Query:  107 RIGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKKEGDLPLFGFLDPESFVNSIXKPRVXIMXC*AGAPCDEXHXTLSAYMEKGDXIIDGGYEWYENTERGEKA 445
            +IG+ GLAVMG+NLA NI  +G+ +SV+NR++ K D  VE +K +   P +     E FVNS+ KPR  ++   AG   D    +L   ++ GD +IDGG   Y++T R  KA
Sbjct:    4 QIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEESKGKNIHPTYSL---EEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNTNYQDTIRRNKA 113

HSP 2 Score: 35.8094 bits (81), Expect = 1.203e-20
Identity = 22/67 (32.84%), Postives = 34/67 (50.75%), Query Frame = 3 
Query:  471 GMGISSGEESAKNGSSLMPGRSFQGYNLLKTFS*RRGFKS*YTAPCELQL-QRGSGYFGKIILLGLK 668
            GMG+S GE  A  G SLMPG   + YN +         K+   A C   +   G+G++ K++  G++
Sbjct:  123 GMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAAKAKDGASCVTYIGPNGAGHYVKMVHNGIE 189
[Close]
BLAST of EY736404 vs. ExPASy Swiss-Prot
Match: 6PGD_STAAS (6-phosphogluconate dehydrogenase, decarboxylating OS=Staphylococcus aureus (strain MSSA476) GN=gnd PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.203e-20
Identity = 47/113 (41.59%), Postives = 68/113 (60.18%), Query Frame = 2 
Query:  107 RIGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKKEGDLPLFGFLDPESFVNSIXKPRVXIMXC*AGAPCDEXHXTLSAYMEKGDXIIDGGYEWYENTERGEKA 445
            +IG+ GLAVMG+NLA NI  +G+ +SV+NR++ K D  VE +K +   P +     E FVNS+ KPR  ++   AG   D    +L   ++ GD +IDGG   Y++T R  KA
Sbjct:    4 QIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEESKGKNIHPTYSL---EEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNTNYQDTIRRNKA 113

HSP 2 Score: 35.8094 bits (81), Expect = 1.203e-20
Identity = 22/67 (32.84%), Postives = 34/67 (50.75%), Query Frame = 3 
Query:  471 GMGISSGEESAKNGSSLMPGRSFQGYNLLKTFS*RRGFKS*YTAPCELQL-QRGSGYFGKIILLGLK 668
            GMG+S GE  A  G SLMPG   + YN +         K+   A C   +   G+G++ K++  G++
Sbjct:  123 GMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAAKAKDGASCVTYIGPNGAGHYVKMVHNGIE 189
[Close]
BLAST of EY736404 vs. ExPASy Swiss-Prot
Match: 6PGD_STAAR (6-phosphogluconate dehydrogenase, decarboxylating OS=Staphylococcus aureus (strain MRSA252) GN=gnd PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.203e-20
Identity = 47/113 (41.59%), Postives = 68/113 (60.18%), Query Frame = 2 
Query:  107 RIGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKKEGDLPLFGFLDPESFVNSIXKPRVXIMXC*AGAPCDEXHXTLSAYMEKGDXIIDGGYEWYENTERGEKA 445
            +IG+ GLAVMG+NLA NI  +G+ +SV+NR++ K D  VE +K +   P +     E FVNS+ KPR  ++   AG   D    +L   ++ GD +IDGG   Y++T R  KA
Sbjct:    4 QIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEESKGKNIHPTYSL---EEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNTNYQDTIRRNKA 113

HSP 2 Score: 35.8094 bits (81), Expect = 1.203e-20
Identity = 22/67 (32.84%), Postives = 34/67 (50.75%), Query Frame = 3 
Query:  471 GMGISSGEESAKNGSSLMPGRSFQGYNLLKTFS*RRGFKS*YTAPCELQL-QRGSGYFGKIILLGLK 668
            GMG+S GE  A  G SLMPG   + YN +         K+   A C   +   G+G++ K++  G++
Sbjct:  123 GMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAAKAKDGASCVTYIGPNGAGHYVKMVHNGIE 189
[Close]
BLAST of EY736404 vs. ExPASy Swiss-Prot
Match: 6PGD_STAAN (6-phosphogluconate dehydrogenase, decarboxylating OS=Staphylococcus aureus (strain N315) GN=gnd PE=1 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.203e-20
Identity = 47/113 (41.59%), Postives = 68/113 (60.18%), Query Frame = 2 
Query:  107 RIGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKKEGDLPLFGFLDPESFVNSIXKPRVXIMXC*AGAPCDEXHXTLSAYMEKGDXIIDGGYEWYENTERGEKA 445
            +IG+ GLAVMG+NLA NI  +G+ +SV+NR++ K D  VE +K +   P +     E FVNS+ KPR  ++   AG   D    +L   ++ GD +IDGG   Y++T R  KA
Sbjct:    4 QIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEESKGKNIHPTYSL---EEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNTNYQDTIRRNKA 113

HSP 2 Score: 35.8094 bits (81), Expect = 1.203e-20
Identity = 22/67 (32.84%), Postives = 34/67 (50.75%), Query Frame = 3 
Query:  471 GMGISSGEESAKNGSSLMPGRSFQGYNLLKTFS*RRGFKS*YTAPCELQL-QRGSGYFGKIILLGLK 668
            GMG+S GE  A  G SLMPG   + YN +         K+   A C   +   G+G++ K++  G++
Sbjct:  123 GMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAAKAKDGASCVTYIGPNGAGHYVKMVHNGIE 189
[Close]
BLAST of EY736404 vs. ExPASy Swiss-Prot
Match: 6PGD_STAAC (6-phosphogluconate dehydrogenase, decarboxylating OS=Staphylococcus aureus (strain COL) GN=gnd PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.203e-20
Identity = 47/113 (41.59%), Postives = 68/113 (60.18%), Query Frame = 2 
Query:  107 RIGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKKEGDLPLFGFLDPESFVNSIXKPRVXIMXC*AGAPCDEXHXTLSAYMEKGDXIIDGGYEWYENTERGEKA 445
            +IG+ GLAVMG+NLA NI  +G+ +SV+NR++ K D  VE +K +   P +     E FVNS+ KPR  ++   AG   D    +L   ++ GD +IDGG   Y++T R  KA
Sbjct:    4 QIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEESKGKNIHPTYSL---EEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNTNYQDTIRRNKA 113

HSP 2 Score: 35.8094 bits (81), Expect = 1.203e-20
Identity = 22/67 (32.84%), Postives = 34/67 (50.75%), Query Frame = 3 
Query:  471 GMGISSGEESAKNGSSLMPGRSFQGYNLLKTFS*RRGFKS*YTAPCELQL-QRGSGYFGKIILLGLK 668
            GMG+S GE  A  G SLMPG   + YN +         K+   A C   +   G+G++ K++  G++
Sbjct:  123 GMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAAKAKDGASCVTYIGPNGAGHYVKMVHNGIE 189
[Close]
BLAST of EY736404 vs. ExPASy Swiss-Prot
Match: 6PGD_SALTY (6-phosphogluconate dehydrogenase, decarboxylating OS=Salmonella typhimurium GN=gnd PE=3 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.203e-20
Identity = 45/109 (41.28%), Postives = 68/109 (62.39%), Query Frame = 2 
Query:  107 RIGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKKEGDLPLFGFLDPESFVNSIXKPRVXIMXC*AGAPCDEXHXTLSAYMEKGDXIIDGGYEWYENTER 433
            +IG+ G+AVMG+NLALNI  +G+ +SV+NR+  K +E +  A+  G   L  +   + FV S+  PR  ++   AGA  D    +L  Y+EKGD IIDGG  ++++T R
Sbjct:    5 QIGVVGMAVMGRNLALNIESRGYTVSVFNRSREKTEEVI--AENPGK-KLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLEKGDIIIDGGNTFFQDTIR 110

HSP 2 Score: 38.5058 bits (88), Expect = 1.203e-20
Identity = 24/79 (30.38%), Postives = 38/79 (48.10%), Query Frame = 3 
Query:  456 GFALPGMGISSGEESAKNGSSLMPGRSFQGYNLLKTFS*RRGFKS*YTAPCELQL-QRGSGYFGKIILLGLKSGYSPLL 689
            GF   G G+S GEE A  G S+MPG     Y L+     +    +    PC   +   G+G++ K++  G++ G   L+
Sbjct:  119 GFNFIGTGVSGGEEGALKGPSIMPGGQKDAYELVAPILTKIAAVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLI 197
[Close]
BLAST of EY736404 vs. ExPASy Swiss-Prot
Match: 6PGD_KLEPN (6-phosphogluconate dehydrogenase, decarboxylating OS=Klebsiella pneumoniae GN=gnd PE=3 SV=2)

HSP 1 Score: 82.4185 bits (202), Expect = 1.203e-20
Identity = 44/109 (40.37%), Postives = 68/109 (62.39%), Query Frame = 2 
Query:  107 RIGLAGLAVMGQNLALNIAEKGFPISVYNRTTSKVDETVERAKKEGDLPLFGFLDPESFVNSIXKPRVXIMXC*AGAPCDEXHXTLSAYMEKGDXIIDGGYEWYENTER 433
            +IG+ G+AVMG+NLALNI  +G+ +SV+NR+  K +E +  A+  G   L  +   + FV S+  PR  ++   AGA  D    +L  Y++KGD IIDGG  ++++T R
Sbjct:    5 QIGVVGMAVMGRNLALNIESRGYTVSVFNRSREKTEEVI--AENTGK-KLVPYYTVQEFVESLETPRRILLMVKAGAGTDSAIDSLKPYLDKGDIIIDGGNTFFQDTIR 110

HSP 2 Score: 38.891 bits (89), Expect = 1.203e-20
Identity = 24/79 (30.38%), Postives = 39/79 (49.37%), Query Frame = 3 
Query:  456 GFALPGMGISSGEESAKNGSSLMPGRSFQGYNLLKTFS*RRGFKS*YTAPCELQL-QRGSGYFGKIILLGLKSGYSPLL 689
            GF   G G+S GEE A  G S+MPG   + Y L+     +    +    PC   +   G+G++ K++  G++ G   L+
Sbjct:  119 GFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILKQIAAVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLI 197
The following BLAST results are available for this feature:
BLAST of EY736404 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 54
Match NameE-valueIdentityDescription
6PGD_HAEIN1.127e-2143.756-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD1_YEAST1.466e-2142.066-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_BUCAI4.201e-2139.826-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_STAAW1.203e-2041.596-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_STAAS1.203e-2041.596-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_STAAR1.203e-2041.596-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_STAAN1.203e-2041.596-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_STAAC1.203e-2041.596-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_SALTY1.203e-2041.286-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_KLEPN1.203e-2040.376-phosphogluconate dehydrogenase, decarboxylating ... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-705-015-H07-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY736404 ID=EY736404; Name=EY736404; organism=Citrus sinensis; type=EST; length=700bp
CACGCGTCCGCTGAATTTTCCTTCTCTTGTTTTTGCATCAGATTTTAGAA
GAAGAAGAAGAAGAATTTTTTAAGAGGAAGATATGGTTGAGGGCAAACAA
CTTACGAGAATAGGCCTTGCTGGTCTGGCGGTCATGGGCCAAAATCTCGC
CCTCAACATTGCTGAAAAAGGCTTTCCCATTTCTGTTTATAACCGAACTA
CCTCCAAAGTTGATGAGACAGTTGAAAGAGCTAAAAAGGAGGGAGATCTG
CCTTTATTTGGTTTCCTTGATCCTGAATCGTTTGTCAATTCAATCCANAA
ACCTCGGGTGANTATCATGNCTTGTTAAGCTGGTGCACCCTGTGACGAAA
NNCATNAGACCCTCTCCGCTTACATGGAGAAAGGTGATNGCATCATTGAT
GGGGGGTATGAGTGGTATGAAAACACCGAGAGAGGAGAAAAAGCATGGCT
GAATTGGGTTTGCTCTACCTGGAATGGGAATTTCAAGTGGTGAAGAAAGT
GCCAAAAATGGTTCCTCCTTGATGCCTGGAAGGTCATTCCAGGGTTACAA
TTTATTGAAAACATTCTCTTAAAGGCGCGGGTTCAAATCCTGATATACGG
CCCCGTGTGAACTTCAATTACAAAGGGGGTCTGGGTATTTTGGGAAAATA
ATCCTATTGGGATTGAAATCGGGATATTCCCCCTTATTGCCAAGGGGTTT
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