EY735761

Overview
NameEY735761
Unique NameEY735761
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length847
Libraries
Library NameType
Sweet orange fruit, development stadium (6 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY735761 vs. ExPASy Swiss-Prot
Match: GSK3B_MOUSE (Glycogen synthase kinase-3 beta OS=Mus musculus GN=Gsk3b PE=1 SV=2)

HSP 1 Score: 94.7449 bits (234), Expect = 6.708e-19
Identity = 51/101 (50.50%), Postives = 60/101 (59.41%), Query Frame = 3 
Query:  351 DLQVNPHTHQLKICDFGSAKMLVCSLFGKKFWEFLKYYC*CLENHDC*DSFSNLQVPGEPNISYICSRYYRAPELIFGATEYTTAIDMWSIGCVLAXLLLG 653
            +L ++P T  LK+CDFGSAK LV                                  GEPN+SYICSRYYRAPELIFGAT+YT++ID+WS GCVLA LLLG
Sbjct:  186 NLLLDPDTAVLKLCDFGSAKQLV---------------------------------RGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLG 253
[Close]
BLAST of EY735761 vs. ExPASy Swiss-Prot
Match: GSK3B_HUMAN (Glycogen synthase kinase-3 beta OS=Homo sapiens GN=GSK3B PE=1 SV=2)

HSP 1 Score: 94.7449 bits (234), Expect = 6.708e-19
Identity = 51/101 (50.50%), Postives = 60/101 (59.41%), Query Frame = 3 
Query:  351 DLQVNPHTHQLKICDFGSAKMLVCSLFGKKFWEFLKYYC*CLENHDC*DSFSNLQVPGEPNISYICSRYYRAPELIFGATEYTTAIDMWSIGCVLAXLLLG 653
            +L ++P T  LK+CDFGSAK LV                                  GEPN+SYICSRYYRAPELIFGAT+YT++ID+WS GCVLA LLLG
Sbjct:  186 NLLLDPDTAVLKLCDFGSAKQLV---------------------------------RGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLG 253
[Close]
BLAST of EY735761 vs. ExPASy Swiss-Prot
Match: GSK3_SCHPO (Protein kinase gsk3 OS=Schizosaccharomyces pombe GN=gsk3 PE=1 SV=3)

HSP 1 Score: 92.4337 bits (228), Expect = 3.329e-18
Identity = 49/101 (48.51%), Postives = 59/101 (58.42%), Query Frame = 3 
Query:  351 DLQVNPHTHQLKICDFGSAKMLVCSLFGKKFWEFLKYYC*CLENHDC*DSFSNLQVPGEPNISYICSRYYRAPELIFGATEYTTAIDMWSIGCVLAXLLLG 653
            +L ++P    LK+CDFGSAK+LV                                  GEPN+SYICSRYYRAPELIFGAT+YT AID+WS GCV+A L+LG
Sbjct:  162 NLLLDPENGILKLCDFGSAKILVA---------------------------------GEPNVSYICSRYYRAPELIFGATDYTHAIDIWSTGCVMAELMLG 229
[Close]
BLAST of EY735761 vs. ExPASy Swiss-Prot
Match: MDS1_YEAST (Serine/threonine-protein kinase MDS1/RIM11 OS=Saccharomyces cerevisiae GN=MDS1 PE=1 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 4.348e-18
Identity = 50/101 (49.50%), Postives = 56/101 (55.45%), Query Frame = 3 
Query:  351 DLQVNPHTHQLKICDFGSAKMLVCSLFGKKFWEFLKYYC*CLENHDC*DSFSNLQVPGEPNISYICSRYYRAPELIFGATEYTTAIDMWSIGCVLAXLLLG 653
            +L V+P T  LK+CDFGSAK L                                  P EPN+SYICSRYYRAPELIFGAT YT  ID+WS GCV+A LLLG
Sbjct:  169 NLLVDPETWSLKLCDFGSAKQLK---------------------------------PTEPNVSYICSRYYRAPELIFGATNYTNQIDIWSSGCVMAELLLG 236
[Close]
BLAST of EY735761 vs. ExPASy Swiss-Prot
Match: SGG_DROME (Protein kinase shaggy OS=Drosophila melanogaster GN=sgg PE=1 SV=2)

HSP 1 Score: 91.6633 bits (226), Expect = 5.678e-18
Identity = 50/101 (49.50%), Postives = 57/101 (56.44%), Query Frame = 3 
Query:  351 DLQVNPHTHQLKICDFGSAKMLVCSLFGKKFWEFLKYYC*CLENHDC*DSFSNLQVPGEPNISYICSRYYRAPELIFGATEYTTAIDMWSIGCVLAXLLLG 653
            +L ++P T  LK+CDFGSAK L+                                  GEPN+SYICSRYYRAPELIFGA  YTT ID+WS GCVLA LLLG
Sbjct:  737 NLLLDPETAVLKLCDFGSAKQLL---------------------------------HGEPNVSYICSRYYRAPELIFGAINYTTKIDVWSAGCVLAELLLG 804
[Close]
BLAST of EY735761 vs. ExPASy Swiss-Prot
Match: GSK3_DICDI (Glycogen synthase kinase-3 OS=Dictyostelium discoideum GN=gskA PE=1 SV=2)

HSP 1 Score: 91.6633 bits (226), Expect = 5.678e-18
Identity = 55/132 (41.67%), Postives = 71/132 (53.79%), Query Frame = 3 
Query:  282 IFLKIFVW-LLNIVSKISSLNS*CDLQVNPH-------THQLKICDFGSAKMLVCSLFGKKFWEFLKYYC*CLENHDC*DSFSNLQVPGEPNISYICSRYYRAPELIFGATEYTTAIDMWSIGCVLAXLLLG 653
            IF+K++++ L   ++ I SL   C   + P        T  LK+CDFGSAK+L+                                  GE N+SYICSR+YRAPELIFG+T YTT ID+WS+GCVLA LLLG
Sbjct:  154 IFVKLYIYQLCRSINYIHSLGI-CHRDIKPQNLLLDTSTSTLKLCDFGSAKILI---------------------------------KGETNVSYICSRHYRAPELIFGSTNYTTTIDVWSLGCVLAELLLG 251
[Close]
BLAST of EY735761 vs. ExPASy Swiss-Prot
Match: GSK3H_DROME (Putative glycogen synthase kinase-3 homolog OS=Drosophila melanogaster GN=gskt PE=2 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 9.686e-18
Identity = 54/131 (41.22%), Postives = 70/131 (53.44%), Query Frame = 3 
Query:  285 FLKIFVW-LLNIVSKISSLNS*CDLQVNPH-------THQLKICDFGSAKMLVCSLFGKKFWEFLKYYC*CLENHDC*DSFSNLQVPGEPNISYICSRYYRAPELIFGATEYTTAIDMWSIGCVLAXLLLG 653
            F++++++ LL  +  + SL   C   + P        T  LK+CDFGSAK L+                                  GEPN+SYICSRYYRAPELIFG+T+YTT IDMWS GCV++ LLLG
Sbjct:  134 FVRLYMYQLLRSMGYLHSLGF-CHRDIKPQNMLLDSETGVLKLCDFGSAKQLIS---------------------------------GEPNVSYICSRYYRAPELIFGSTDYTTKIDMWSAGCVMSELLLG 230
[Close]
BLAST of EY735761 vs. ExPASy Swiss-Prot
Match: GSK3_CAEBR (Glycogen synthase kinase-3 OS=Caenorhabditis briggsae GN=gsk-3 PE=3 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.071e-16
Identity = 55/132 (41.67%), Postives = 73/132 (55.30%), Query Frame = 3 
Query:  282 IFLKIFVW-LLNIVSKISSLNS*C-------DLQVNPHTHQLKICDFGSAKMLVCSLFGKKFWEFLKYYC*CLENHDC*DSFSNLQVPGEPNISYICSRYYRAPELIFGATEYTTAIDMWSIGCVLAXLLLG 653
            I++K++++ LL  ++ I S+   C       +L ++P T  LK+CDFGSAK LV +                                 EPN+SYICSRYYRAPELIFGAT YT +ID+WS G V+A LLLG
Sbjct:  136 IYVKLYMYQLLRSLAYIHSIGI-CHRDIKPQNLLIDPETGILKLCDFGSAKYLVRN---------------------------------EPNVSYICSRYYRAPELIFGATNYTNSIDVWSAGTVIAELLLG 233
[Close]
BLAST of EY735761 vs. ExPASy Swiss-Prot
Match: GSK31_SCHPO (Protein kinase gsk31 OS=Schizosaccharomyces pombe GN=gsk31 PE=1 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.071e-16
Identity = 47/101 (46.53%), Postives = 56/101 (55.45%), Query Frame = 3 
Query:  351 DLQVNPHTHQLKICDFGSAKMLVCSLFGKKFWEFLKYYC*CLENHDC*DSFSNLQVPGEPNISYICSRYYRAPELIFGATEYTTAIDMWSIGCVLAXLLLG 653
            +L V+  T  LK+CDFGSAK+LV                                 P EPN+SYICSRYYRAPEL+FGAT YTT ID+WS  CV+A L +G
Sbjct:  155 NLLVDYKTGILKLCDFGSAKVLV---------------------------------PSEPNVSYICSRYYRAPELVFGATHYTTKIDVWSAACVIAELFIG 222
[Close]
BLAST of EY735761 vs. ExPASy Swiss-Prot
Match: GSK3_CAEEL (Glycogen synthase kinase-3 OS=Caenorhabditis elegans GN=gsk-3 PE=1 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 2.386e-16
Identity = 54/132 (40.91%), Postives = 73/132 (55.30%), Query Frame = 3 
Query:  282 IFLKIFVW-LLNIVSKISSLNS*C-------DLQVNPHTHQLKICDFGSAKMLVCSLFGKKFWEFLKYYC*CLENHDC*DSFSNLQVPGEPNISYICSRYYRAPELIFGATEYTTAIDMWSIGCVLAXLLLG 653
            I++K++++ LL  ++ I S+   C       +L ++P +  LK+CDFGSAK LV +                                 EPN+SYICSRYYRAPELIFGAT YT +ID+WS G V+A LLLG
Sbjct:  136 IYVKLYMYQLLRSLAYIHSIGI-CHRDIKPQNLLIDPESGVLKLCDFGSAKYLVRN---------------------------------EPNVSYICSRYYRAPELIFGATNYTNSIDVWSAGTVMAELLLG 233
The following BLAST results are available for this feature:
BLAST of EY735761 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 33
Match NameE-valueIdentityDescription
GSK3B_MOUSE6.708e-1950.50Glycogen synthase kinase-3 beta OS=Mus musculus GN... [more]
GSK3B_HUMAN6.708e-1950.50Glycogen synthase kinase-3 beta OS=Homo sapiens GN... [more]
GSK3_SCHPO3.329e-1848.51Protein kinase gsk3 OS=Schizosaccharomyces pombe G... [more]
MDS1_YEAST4.348e-1849.50Serine/threonine-protein kinase MDS1/RIM11 OS=Sacc... [more]
SGG_DROME5.678e-1849.50Protein kinase shaggy OS=Drosophila melanogaster G... [more]
GSK3_DICDI5.678e-1841.67Glycogen synthase kinase-3 OS=Dictyostelium discoi... [more]
GSK3H_DROME9.686e-1841.22Putative glycogen synthase kinase-3 homolog OS=Dro... [more]
GSK3_CAEBR1.071e-1641.67Glycogen synthase kinase-3 OS=Caenorhabditis brigg... [more]
GSK31_SCHPO1.071e-1646.53Protein kinase gsk31 OS=Schizosaccharomyces pombe ... [more]
GSK3_CAEEL2.386e-1640.91Glycogen synthase kinase-3 OS=Caenorhabditis elega... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-705-004-G02-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY735761 ID=EY735761; Name=EY735761; organism=Citrus sinensis; type=EST; length=847bp
CACGCGTCCGCTTAATTGGTCTGAGCTCTGTTGTAGACTAATTAGTTTTC
TGTTAGTGAGAAAAATTTGTTTCGATGAGCAGATGTAAGGTTTTGATAGT
AAGCTCCTTGGCACTTATTGCTAGGTATGCACACAAGACCGCCTTTCTAA
AAAAATAAAAAATTAAAATGACGTGGGTGTAAAATTTCTTTAAGGTTTTA
CTCGAAATTTAATAGTGTTGTTATAGGTTTTTGCTTGTGAAAGTTTGAGA
TGATGATCTAGATATGCTTCATTATCATGCTATCTTTCTTAAAATTTTTG
TTTGGTTATTGAATATAGTATCAAAAATATCTAGTTTGAATTCGTAATGT
GATTTGCAGGTCAATCCCCATACACACCAGTTAAAGATATGTGACTTTGG
AAGTGCAAAGATGTTGGTATGTTCTTTATTTGGGAAAAAGTTTTGGGAAT
TTTTGAAGTACTATTGCTAATGCTTGGAAAATCATGACTGCTGAGATTCA
TTCTCAAATCTCCAGGTGCCAGGTGAACCCAACATATCGTATATCTGCTC
AAGATATTACAGGGCCCCGGAACTTATATTTGGGGCTACAGAATACACAA
CTGCCATTGATATGTGGTCCATTGGATGTGTCTTGGCTGANCTTCTTCTT
GGGACACGTTGGTGTGTGCTTTTTATTTTCATCTGGAATCTGTTTGAATG
TTGGTGTCAAATTTCACCCGCCCTTGATAATTTGTCAGGGGGGGCCTATT
GGGAGAGGTTCCAGGAAGGCGATGAGCTTAACCTGGGGGTTCAAGAATTC
GATGAGGTGGTCGGAAGGGCCCGAGGTGGAGAGGGGGGTGCGGGGCG
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