EY728898

Overview
NameEY728898
Unique NameEY728898
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length917
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY728898 vs. ExPASy Swiss-Prot
Match: RL18_THEEB (50S ribosomal protein L18 OS=Thermosynechococcus elongatus (strain BP-1) GN=rplR PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 8.040e-24
Identity = 33/67 (49.25%), Postives = 50/67 (74.63%), Query Frame = 3 
Query:  219 RTKREDRTARHSRIRKKIDGTPERPRLCVFRSNKHLYVQVIDDTKMHTLASASTMQKPISEELDYSS 419
            +T+   R +RH RIR+K+ GT +RPRL VFRS++H+Y QVIDDT+ HTL +AS+++  + ++L   S
Sbjct:    3 QTRTAARQSRHQRIRRKVKGTSDRPRLAVFRSHQHIYAQVIDDTRHHTLVAASSLEPELRQKLGKGS 69

HSP 2 Score: 56.6102 bits (135), Expect = 8.040e-24
Identity = 27/44 (61.36%), Postives = 32/44 (72.73%), Query Frame = 2 
Query:  443 VGEMIAKSCLEKGITKVAFDRGGYPYHGRIQALADAAREHGLQF 574
            VG +IA+     GI +V FDRGG  YHGR++ALADAARE GL F
Sbjct:   77 VGRLIAERAKAAGIERVVFDRGGNIYHGRVKALADAAREGGLDF 120
[Close]
BLAST of EY728898 vs. ExPASy Swiss-Prot
Match: RL18_ACIC1 (50S ribosomal protein L18 OS=Acidothermus cellulolyticus (strain ATCC 43068 / 11B) GN=rplR PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.028e-23
Identity = 34/58 (58.62%), Postives = 44/58 (75.86%), Query Frame = 3 
Query:  213 RPRTKREDRTARHSRIRKKIDGTPERPRLCVFRSNKHLYVQVIDDTKMHTLASASTMQ 386
            R  T+   R  RH R+RKKI GTPERPRL V RS +H++VQ++DDT+ HTLASAS+ +
Sbjct:   11 RGSTRTAARMRRHFRVRKKISGTPERPRLVVHRSARHVFVQIVDDTRGHTLASASSFE 68

HSP 2 Score: 57.7658 bits (138), Expect = 1.028e-23
Identity = 26/45 (57.78%), Postives = 33/45 (73.33%), Query Frame = 2 
Query:  434 AKKVGEMIAKSCLEKGITKVAFDRGGYPYHGRIQALADAAREHGL 568
            A++VG++IA+     GIT V FDR GY YHGR+ A+AD ARE GL
Sbjct:   82 ARRVGQLIAERAKAAGITTVVFDRAGYRYHGRVAAIADGAREGGL 126
[Close]
BLAST of EY728898 vs. ExPASy Swiss-Prot
Match: RL18_CLOCE (50S ribosomal protein L18 OS=Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) GN=rplR PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.037e-23
Identity = 31/47 (65.96%), Postives = 38/47 (80.85%), Query Frame = 2 
Query:  434 AKKVGEMIAKSCLEKGITKVAFDRGGYPYHGRIQALADAAREHGLQF 574
            AK+VG+++A   ++KGI KV FDRGGY YHGRI+ LADAARE GL F
Sbjct:   76 AKEVGKLVASKAIDKGIKKVVFDRGGYIYHGRIKELADAAREAGLDF 122

HSP 2 Score: 63.1586 bits (152), Expect = 1.037e-23
Identity = 30/49 (61.22%), Postives = 38/49 (77.55%), Query Frame = 3 
Query:  237 RTARHSRIRKKIDGTPERPRLCVFRSNKHLYVQVIDDTKMHTLASASTM 383
            R  +H R+RKKI GT ERPR+ VFRS  ++YVQ+IDDT  +TL SAST+
Sbjct:   11 RLRKHLRVRKKITGTAERPRMNVFRSLNNIYVQIIDDTTGNTLVSASTL 59
[Close]
BLAST of EY728898 vs. ExPASy Swiss-Prot
Match: RL18_CLOBM (50S ribosomal protein L18 OS=Clostridium botulinum (strain Loch Maree / Type A3) GN=rplR PE=3 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.046e-23
Identity = 30/55 (54.55%), Postives = 40/55 (72.73%), Query Frame = 3 
Query:  225 KREDRTARHSRIRKKIDGTPERPRLCVFRSNKHLYVQVIDDTKMHTLASASTMQK 389
            + + R  RH R+RKKI GT ERPRL V+RS KH+Y Q+IDD +  TL +AS+ +K
Sbjct:    7 RSQSRERRHMRVRKKIFGTAERPRLSVYRSEKHIYAQLIDDVEGKTLVAASSSEK 61

HSP 2 Score: 65.855 bits (159), Expect = 1.046e-23
Identity = 31/47 (65.96%), Postives = 37/47 (78.72%), Query Frame = 2 
Query:  434 AKKVGEMIAKSCLEKGITKVAFDRGGYPYHGRIQALADAAREHGLQF 574
            AK VG+MIA+  LEKG+ KV FDRGG+ YHGRI+ LA+ ARE GL F
Sbjct:   73 AKLVGKMIAEKALEKGLKKVVFDRGGFIYHGRIKELAEGAREAGLDF 119
[Close]
BLAST of EY728898 vs. ExPASy Swiss-Prot
Match: RL18_DEHSC (50S ribosomal protein L18 OS=Dehalococcoides sp. (strain CBDB1) GN=rplR PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 1.362e-23
Identity = 33/61 (54.10%), Postives = 40/61 (65.57%), Query Frame = 3 
Query:  228 REDRTARHSRIRKKIDGTPERPRLCVFRSNKHLYVQVIDDTKMHTLASASTMQKPISEELD 410
            RE R  RH R+RKK+ GT  RPRLCVFRS +++Y QVI+D    TL  AST    +  ELD
Sbjct:    7 REARIVRHERLRKKVSGTEARPRLCVFRSIENIYTQVINDNCGSTLVQASTKDAELKAELD 67

HSP 2 Score: 63.5438 bits (153), Expect = 1.362e-23
Identity = 28/44 (63.64%), Postives = 36/44 (81.82%), Query Frame = 2 
Query:  443 VGEMIAKSCLEKGITKVAFDRGGYPYHGRIQALADAAREHGLQF 574
            +G +IAK  LE GI++V FDRGGY YHGR++ALA+AAR  GL+F
Sbjct:   78 IGNLIAKRSLEAGISEVVFDRGGYKYHGRVKALAEAARSGGLKF 121
[Close]
BLAST of EY728898 vs. ExPASy Swiss-Prot
Match: RL18_DEHSB (50S ribosomal protein L18 OS=Dehalococcoides sp. (strain BAV1) GN=rplR PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 1.362e-23
Identity = 33/61 (54.10%), Postives = 40/61 (65.57%), Query Frame = 3 
Query:  228 REDRTARHSRIRKKIDGTPERPRLCVFRSNKHLYVQVIDDTKMHTLASASTMQKPISEELD 410
            RE R  RH R+RKK+ GT  RPRLCVFRS +++Y QVI+D    TL  AST    +  ELD
Sbjct:    7 REARIVRHERLRKKVSGTEARPRLCVFRSIENIYTQVINDNCGSTLVQASTKDAELKAELD 67

HSP 2 Score: 63.5438 bits (153), Expect = 1.362e-23
Identity = 28/44 (63.64%), Postives = 36/44 (81.82%), Query Frame = 2 
Query:  443 VGEMIAKSCLEKGITKVAFDRGGYPYHGRIQALADAAREHGLQF 574
            +G +IAK  LE GI++V FDRGGY YHGR++ALA+AAR  GL+F
Sbjct:   78 IGNLIAKRSLEAGISEVVFDRGGYKYHGRVKALAEAARSGGLKF 121
[Close]
BLAST of EY728898 vs. ExPASy Swiss-Prot
Match: RL18_DEHE1 (50S ribosomal protein L18 OS=Dehalococcoides ethenogenes (strain 195) GN=rplR PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 1.362e-23
Identity = 33/61 (54.10%), Postives = 40/61 (65.57%), Query Frame = 3 
Query:  228 REDRTARHSRIRKKIDGTPERPRLCVFRSNKHLYVQVIDDTKMHTLASASTMQKPISEELD 410
            RE R  RH R+RKK+ GT  RPRLCVFRS +++Y QVI+D    TL  AST    +  ELD
Sbjct:    7 REARIVRHERLRKKVSGTEARPRLCVFRSIENIYTQVINDNCGSTLVQASTKDAELKAELD 67

HSP 2 Score: 63.5438 bits (153), Expect = 1.362e-23
Identity = 28/44 (63.64%), Postives = 36/44 (81.82%), Query Frame = 2 
Query:  443 VGEMIAKSCLEKGITKVAFDRGGYPYHGRIQALADAAREHGLQF 574
            +G +IAK  LE GI++V FDRGGY YHGR++ALA+AAR  GL+F
Sbjct:   78 IGNLIAKRSLEAGISEVVFDRGGYKYHGRVKALAEAARSGGLKF 121
[Close]
BLAST of EY728898 vs. ExPASy Swiss-Prot
Match: RL18_OENOB (50S ribosomal protein L18 OS=Oenococcus oeni (strain BAA-331 / PSU-1) GN=rplR PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.773e-23
Identity = 35/60 (58.33%), Postives = 44/60 (73.33%), Query Frame = 3 
Query:  210 TRPRTKREDRTARHSRIRKKIDGTPERPRLCVFRSNKHLYVQVIDDTKMHTLASASTMQK 389
            T+P  K + R  RH+R+R KI GT +RPRL VFRSN ++Y QVIDD    TLASAST++K
Sbjct:    3 TKP-DKNKTRQKRHARVRGKISGTAQRPRLSVFRSNSNIYAQVIDDVAGVTLASASTLEK 61

HSP 2 Score: 62.003 bits (149), Expect = 1.773e-23
Identity = 28/47 (59.57%), Postives = 36/47 (76.60%), Query Frame = 2 
Query:  434 AKKVGEMIAKSCLEKGITKVAFDRGGYPYHGRIQALADAAREHGLQF 574
            A KVG+ +AK  + K IT+V FDR GY YHGR+++LAD ARE GL+F
Sbjct:   72 ASKVGQSVAKLAIAKKITEVVFDRSGYLYHGRVRSLADGAREAGLKF 118
[Close]
BLAST of EY728898 vs. ExPASy Swiss-Prot
Match: RL18_CLOB6 (50S ribosomal protein L18 OS=Clostridium botulinum (strain 657 / Type Ba4) GN=rplR PE=3 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.773e-23
Identity = 30/55 (54.55%), Postives = 40/55 (72.73%), Query Frame = 3 
Query:  225 KREDRTARHSRIRKKIDGTPERPRLCVFRSNKHLYVQVIDDTKMHTLASASTMQK 389
            + + R  RH R+RKKI GT ERPRL V+RS KH+Y Q+IDD +  TL +AS+ +K
Sbjct:    7 RSQSRERRHMRVRKKIFGTAERPRLSVYRSEKHIYAQLIDDIEGKTLVAASSAEK 61

HSP 2 Score: 65.4698 bits (158), Expect = 1.773e-23
Identity = 30/47 (63.83%), Postives = 37/47 (78.72%), Query Frame = 2 
Query:  434 AKKVGEMIAKSCLEKGITKVAFDRGGYPYHGRIQALADAAREHGLQF 574
            AK VG+M+A+  LEKG+ KV FDRGG+ YHGRI+ LA+ ARE GL F
Sbjct:   73 AKLVGKMVAEKALEKGLKKVVFDRGGFIYHGRIKELAEGAREAGLDF 119
[Close]
BLAST of EY728898 vs. ExPASy Swiss-Prot
Match: RL18_PROMT (50S ribosomal protein L18 OS=Prochlorococcus marinus (strain NATL2A) GN=rplR PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.286e-23
Identity = 28/67 (41.79%), Postives = 44/67 (65.67%), Query Frame = 3 
Query:  222 TKREDRTARHSRIRKKIDGTPERPRLCVFRSNKHLYVQVIDDTKMHTLASASTMQKPISEELDYSSG 422
            ++++    RH R+R+ + GT  RPRL VFRSN H+Y Q+IDD   +T+ +AST+ K +   L  ++G
Sbjct:    5 SRKQQTQKRHKRLRRNLSGTESRPRLAVFRSNNHIYAQIIDDDAQNTICAASTLDKDLKASLKVNAG 71

HSP 2 Score: 61.6178 bits (148), Expect = 2.286e-23
Identity = 30/51 (58.82%), Postives = 37/51 (72.55%), Query Frame = 2 
Query:  422 SHDFAKKVGEMIAKSCLEKGITKVAFDRGGYPYHGRIQALADAAREHGLQF 574
            S D +  VGE++AK  L KGI +V FDRGG  YHGR++ALA+AAR  GL F
Sbjct:   72 SCDASTAVGELVAKKALSKGIKQVIFDRGGNIYHGRVKALAEAARVAGLNF 122
The following BLAST results are available for this feature:
BLAST of EY728898 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
RL18_THEEB8.040e-2449.2550S ribosomal protein L18 OS=Thermosynechococcus e... [more]
RL18_ACIC11.028e-2358.6250S ribosomal protein L18 OS=Acidothermus cellulol... [more]
RL18_CLOCE1.037e-2365.9650S ribosomal protein L18 OS=Clostridium celluloly... [more]
RL18_CLOBM1.046e-2354.5550S ribosomal protein L18 OS=Clostridium botulinum... [more]
RL18_DEHSC1.362e-2354.1050S ribosomal protein L18 OS=Dehalococcoides sp. (... [more]
RL18_DEHSB1.362e-2354.1050S ribosomal protein L18 OS=Dehalococcoides sp. (... [more]
RL18_DEHE11.362e-2354.1050S ribosomal protein L18 OS=Dehalococcoides ethen... [more]
RL18_OENOB1.773e-2358.3350S ribosomal protein L18 OS=Oenococcus oeni (stra... [more]
RL18_CLOB61.773e-2354.5550S ribosomal protein L18 OS=Clostridium botulinum... [more]
RL18_PROMT2.286e-2341.7950S ribosomal protein L18 OS=Prochlorococcus marin... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-010-A10-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY728898 ID=EY728898; Name=EY728898; organism=Citrus sinensis; type=EST; length=917bp
CCACGCGTCCGCCCACGCGTCCGAACGCTATCCGTTTGGTTCCGAAAGTG
GAAGAAGGAAAGAAAATGGCAGCTGGTGCGAGCTCAGTTTGGATGCTTAA
TCAGAGTTCATTTCTAGGATCATGTCAGCAACTTTCGAGTGTCTCAAAAT
TAGTGGCAAGCAGCAGGCCAGGGCCAGTACCGCCATCTCTTTTCATCGTC
GAAGCCTCGACCAGACCAAGAACCAAAAGAGAGGACCGCACTGCCCGCCA
TTCTCGCATCAGAAAGAAGATTGATGGGACTCCAGAGAGGCCGAGGCTGT
GTGTTTTCCGCTCCAACAAGCATCTCTATGTCCAGGTGATTGATGATACT
AAGATGCATACACTTGCTTCAGCTTCGACGATGCAGAAGCCCATCTCTGA
GGAGCTTGACTATAGTTCTGGTCCCACGATTTTGCAAAGAAGGTGGGTGA
AATGATTGCAAAGTCATGTTTGGAGAAGGGGATCACAAAAGTAGCCTTTG
ATCGAGGTGGGTACCCATACCATGGACGGATACAAGCCCTAGCCGATGCA
GCTCGGGAACATGGTCTTCAATTCTAAGAACTATGTAGTCAATTGTTCAT
TTTTTCCAAAAATCCATTTCTCTGTTCTGTTCTTTGAACTTAATGGGTGG
AAGAAAGCATCAATGTGTGAACAAGATACAATTTCTGACCATCATATGTT
TAGAAGCTCTCTTGTNCAGTTTATCTTTTTGTGAAGTCAGATTGGTTTAG
TTGTTATCGTGCTCTANNNNNNNNNNNNNNNNNANNNNNNNNNNANNNNN
AAGGGGCGGCGCTCTAAGGATCCANCTTAGTACCCGTCATGCACGTCATA
CCCTCCTAAGTGTCCCTAAATCAATCCTGGGCGTGTTTACACGTCTGACT
GGAAAACCGGGGTTCCA
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