EY727817

Overview
NameEY727817
Unique NameEY727817
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length987
Libraries
Library NameType
Sweet orange fruit, development stadium (4 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY727817 vs. ExPASy Swiss-Prot
Match: DPHB_METMP (Probable diphthine synthase OS=Methanococcus maripaludis GN=dphB PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 6.309e-25
Identity = 50/110 (45.45%), Postives = 73/110 (66.36%), Query Frame = 1 
Query:   97 MLYIIGLGLGDERDITLRGLEAVKKCDKVYIEAYTSLLSFGLSTDGLSTLEKLYG---KPIALADREMVEEKADKILSESQESNVAFLVVGDPFGATTHTDLVVRAKKLG 417
            ML + GLGL DERD+TL+ L+  KK DK+Y E YT++L+        +T+EK+ G   KPI + DRE VE + +K++ E+++ ++ FL  GDP  ATTH D+ V A+K G
Sbjct:    1 MLVMAGLGLYDERDVTLKTLDFAKKVDKIYAEFYTAILTG-------TTMEKIEGTLQKPITVLDREKVEYETNKLIDEAKDKDIMFLTAGDPMVATTHVDIAVEARKKG 103

HSP 2 Score: 38.891 bits (89), Expect = 6.309e-25
Identity = 17/34 (50.00%), Postives = 25/34 (73.53%), Query Frame = 2 
Query:  401 ELRNWGIQVKAVHNASVMNAVGICGLQLYRFGET 502
            E R  GI+V  ++  S+ +A+GI GLQLY+FG+T
Sbjct:   98 EARKKGIEVVIINAPSIYSAIGITGLQLYKFGKT 131
[Close]
BLAST of EY727817 vs. ExPASy Swiss-Prot
Match: DPHB_METM7 (Probable diphthine synthase OS=Methanococcus maripaludis (strain C7 / ATCC BAA-1331) GN=dphB PE=3 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.355e-24
Identity = 49/107 (45.79%), Postives = 70/107 (65.42%), Query Frame = 1 
Query:   97 MLYIIGLGLGDERDITLRGLEAVKKCDKVYIEAYTSLLSFGLSTDGLSTLEKLYGKPIALADREMVEEKADKILSESQESNVAFLVVGDPFGATTHTDLVVRAKKLG 417
            ML + GLGL DERD+TL+ L+  KK DK+Y E YT++L+ G S   +  +E    KPI + DRE VE + +K++ E+ + ++ FL  GDP  ATTH D+ V A+K G
Sbjct:    1 MLVMAGLGLYDERDVTLKTLDFAKKVDKIYAEFYTAILT-GTS---MEKIENTLQKPITVLDREKVEYETNKLIEEAVDKDIMFLTAGDPMVATTHVDIAVEARKKG 103

HSP 2 Score: 38.891 bits (89), Expect = 2.355e-24
Identity = 17/34 (50.00%), Postives = 25/34 (73.53%), Query Frame = 2 
Query:  401 ELRNWGIQVKAVHNASVMNAVGICGLQLYRFGET 502
            E R  GI+V  ++  S+ +A+GI GLQLY+FG+T
Sbjct:   98 EARKKGIEVVIINAPSIYSAIGITGLQLYKFGKT 131
[Close]
BLAST of EY727817 vs. ExPASy Swiss-Prot
Match: DPHB_METM5 (Probable diphthine synthase OS=Methanococcus maripaludis (strain C5 / ATCC BAA-1333) GN=dphB PE=3 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.355e-24
Identity = 47/107 (43.93%), Postives = 70/107 (65.42%), Query Frame = 1 
Query:   97 MLYIIGLGLGDERDITLRGLEAVKKCDKVYIEAYTSLLSFGLSTDGLSTLEKLYGKPIALADREMVEEKADKILSESQESNVAFLVVGDPFGATTHTDLVVRAKKLG 417
            ML + GLGL DERD+TL+ L+  KK DK+Y E YT++L+       +  +E+   KPI + DRE VE + +K++ E+++ ++ FL  GDP  ATTH D+ V A+K G
Sbjct:    1 MLVMAGLGLYDERDVTLKTLDFAKKVDKIYAEFYTAILT----GTTMEKVEETLQKPITVLDREKVEYETNKLIEEAKDKDIMFLTAGDPMVATTHVDIAVEARKKG 103

HSP 2 Score: 38.891 bits (89), Expect = 2.355e-24
Identity = 17/34 (50.00%), Postives = 25/34 (73.53%), Query Frame = 2 
Query:  401 ELRNWGIQVKAVHNASVMNAVGICGLQLYRFGET 502
            E R  GI+V  ++  S+ +A+GI GLQLY+FG+T
Sbjct:   98 EARKKGIEVVIINAPSIYSAIGITGLQLYKFGKT 131
[Close]
BLAST of EY727817 vs. ExPASy Swiss-Prot
Match: DPHB_METM6 (Probable diphthine synthase OS=Methanococcus maripaludis (strain C6 / ATCC BAA-1332) GN=dphB PE=3 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.489e-23
Identity = 46/107 (42.99%), Postives = 69/107 (64.49%), Query Frame = 1 
Query:   97 MLYIIGLGLGDERDITLRGLEAVKKCDKVYIEAYTSLLSFGLSTDGLSTLEKLYGKPIALADREMVEEKADKILSESQESNVAFLVVGDPFGATTHTDLVVRAKKLG 417
            ML + GLGL DERD+TL+ L+  KK DK+Y E YT++L+       +  +E+   KPI + +RE VE + +K++ E++  ++ FL  GDP  ATTH D+ V A+K G
Sbjct:    1 MLVMAGLGLYDERDVTLKTLDFAKKVDKIYAEFYTAILT----GTTMEKVEETLQKPITVLNREKVEYETNKLIEEAKNKDIMFLTAGDPMVATTHVDIAVEARKKG 103

HSP 2 Score: 38.891 bits (89), Expect = 1.489e-23
Identity = 17/34 (50.00%), Postives = 25/34 (73.53%), Query Frame = 2 
Query:  401 ELRNWGIQVKAVHNASVMNAVGICGLQLYRFGET 502
            E R  GI+V  ++  S+ +A+GI GLQLY+FG+T
Sbjct:   98 EARKKGIEVVIINAPSIYSAIGITGLQLYKFGKT 131
[Close]
BLAST of EY727817 vs. ExPASy Swiss-Prot
Match: DPHB_PYRHO (Probable diphthine synthase OS=Pyrococcus horikoshii GN=dphB PE=1 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 3.278e-23
Identity = 56/111 (50.45%), Postives = 73/111 (65.77%), Query Frame = 1 
Query:   97 MLYIIGLGLGDERDITLRGLEAVKKCDKVYIEAYTSLLSFGLSTDGLSTLEKLYGKPIALADREMVEEKADKI-LSESQESNVAFLVVGDPFGATTHTDLVVRAKKLGDSS 426
            +LY IGLGL DERDIT++GLE  KKCD V+ E YTSL    ++   L  ++KL GK I +  RE VE   + I L  ++E++VAFL  GDP  ATTH +L +RAK+ G  S
Sbjct:    2 VLYFIGLGLYDERDITVKGLEIAKKCDYVFAEFYTSL----MAGTTLGRIQKLIGKEIRVLSREDVELNFENIVLPLAKENDVAFLTPGDPLVATTHAELRIRAKRAGVES 108

HSP 2 Score: 33.113 bits (74), Expect = 3.278e-23
Identity = 12/29 (41.38%), Postives = 21/29 (72.41%), Query Frame = 2 
Query:  416 GIQVKAVHNASVMNAVGICGLQLYRFGET 502
            G++   +H  S+ +AVGI GL +Y+FG++
Sbjct:  105 GVESYVIHAPSIYSAVGITGLHIYKFGKS 133
[Close]
BLAST of EY727817 vs. ExPASy Swiss-Prot
Match: DPHB_METVS (Probable diphthine synthase OS=Methanococcus vannielii (strain SB / ATCC 35089 / DSM 1224) GN=dphB PE=3 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.224e-22
Identity = 46/107 (42.99%), Postives = 68/107 (63.55%), Query Frame = 1 
Query:   97 MLYIIGLGLGDERDITLRGLEAVKKCDKVYIEAYTSLLSFGLSTDGLSTLEKLYGKPIALADREMVEEKADKILSESQESNVAFLVVGDPFGATTHTDLVVRAKKLG 417
            ML + GLGL DE D+TL+ +E  KK DK+Y E YT++L+       +  +EK   K I + +RE VE + +K++ E++E ++ FL  GDP  ATTH D+ V A+K G
Sbjct:    1 MLVMAGLGLYDELDVTLKTVEFAKKVDKIYAEFYTAILT----GTTIEKIEKTLQKEITVLNREKVEYETNKLIEEAKEKDIMFLTAGDPMVATTHVDIAVEARKKG 103

HSP 2 Score: 37.7354 bits (86), Expect = 1.224e-22
Identity = 17/34 (50.00%), Postives = 25/34 (73.53%), Query Frame = 2 
Query:  401 ELRNWGIQVKAVHNASVMNAVGICGLQLYRFGET 502
            E R  GI+V  ++  S+ +A+GI GLQLY+FG+T
Sbjct:   98 EARKKGIEVIILNAPSIYSAIGITGLQLYKFGKT 131
[Close]
BLAST of EY727817 vs. ExPASy Swiss-Prot
Match: DPHB_UNCMA (Probable diphthine synthase OS=Uncultured methanogenic archaeon RC-I GN=dphB PE=3 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 3.507e-22
Identity = 51/108 (47.22%), Postives = 71/108 (65.74%), Query Frame = 1 
Query:   97 MLYIIGLGLGDERDITLRGLEAVKKCDKVYIEAYTSLLSFGLSTDGLSTLEKLYGKPIALADREMVEE-KADKILSESQESNVAFLVVGDPFGATTHTDLVVRAKKLG 417
            ML  IGLGL D++DI+++GLEA+++ D VY E YTS L        L  +++LYGKP+ +  RE VE+   D +LS++ +  V FL  GD   ATTH DL +RAKK+G
Sbjct:    1 MLTFIGLGLYDQKDISVKGLEAIREADVVYAEFYTSRLMGAT----LEDMQELYGKPVKVLMREDVEQHPKDTVLSDAVDKKVVFLTGGDAMVATTHVDLRLRAKKMG 104

HSP 2 Score: 34.6538 bits (78), Expect = 3.507e-22
Identity = 15/33 (45.45%), Postives = 24/33 (72.73%), Query Frame = 2 
Query:  407 RNWGIQVKAVHNASVMNAV-GICGLQLYRFGET 502
            +  GI+ + +H AS+ +AV G+ GLQ YRFG++
Sbjct:  101 KKMGIETRLIHGASIQSAVCGLTGLQNYRFGKS 133
[Close]
BLAST of EY727817 vs. ExPASy Swiss-Prot
Match: DPHB_PYRFU (Probable diphthine synthase OS=Pyrococcus furiosus GN=dphB PE=3 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 6.350e-22
Identity = 58/110 (52.73%), Postives = 75/110 (68.18%), Query Frame = 1 
Query:  100 LYIIGLGLGDERDITLRGLEAVKKCDKVYIEAYTSLLSFGLSTDGLSTLEKLYGKPIALADREMVEEKADKI-LSESQESNVAFLVVGDPFGATTHTDLVVRAKKLGDSS 426
            LY IGLGL DE+DITL+GLE  K+CDKVY E YTSL    ++   L  +E++ GK I + +RE VE   +KI L E++E  VAFL  GDP  ATTH +L +RAK++G  S
Sbjct:    3 LYFIGLGLYDEKDITLKGLEIAKRCDKVYAEFYTSL----MAGTTLEKIEEVIGKKIIVLNREDVEMNFEKIVLPEAKEKEVAFLTAGDPMVATTHAELRIRAKRMGVES 108
[Close]
BLAST of EY727817 vs. ExPASy Swiss-Prot
Match: DPHB_METTH (Probable diphthine synthase OS=Methanobacterium thermoautotrophicum GN=dphB PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 8.236e-21
Identity = 48/107 (44.86%), Postives = 69/107 (64.49%), Query Frame = 1 
Query:   97 MLYIIGLGLGDERDITLRGLEAVKKCDKVYIEAYTSLLSFGLSTDGLSTLEKLYGKPIALADREMVEEKADKILSESQESNVAFLVVGDPFGATTHTDLVVRAKKLG 417
            MLYIIGLGL DE DI+++GL A++ CD+VY E YT++    L    LS +E+  G+ + +  R  +EE+   I   + E +VA LV GDP  ATTHT+L++ A + G
Sbjct:    1 MLYIIGLGLYDENDISIKGLRAIEDCDEVYAEFYTAM----LQGSSLSAIERRIGRSVKVLGRGEIEEERIPI-ERALELDVALLVAGDPLVATTHTELLIDAHRRG 102

HSP 2 Score: 33.4982 bits (75), Expect = 8.236e-21
Identity = 13/38 (34.21%), Postives = 25/38 (65.79%), Query Frame = 2 
Query:  395 LFELRNWGIQVKAVHNASVMNAV-GICGLQLYRFGETV 505
            L +    GI+ + +H++S+++A  G+ GLQ Y+FG  +
Sbjct:   95 LIDAHRRGIETRVIHSSSIISAAPGLAGLQAYKFGRII 132
[Close]
BLAST of EY727817 vs. ExPASy Swiss-Prot
Match: DPHB_METLZ (Probable diphthine synthase OS=Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) GN=dphB PE=3 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 5.196e-20
Identity = 50/103 (48.54%), Postives = 63/103 (61.17%), Query Frame = 1 
Query:   97 MLYIIGLGLGDERDITLRGLEAVKKCDKVYIEAYTSLLSFGLSTDGLSTLEKLYGKPIALADREMVEEKADKILSESQESNVAFLVVGDPFGATTHTDLVVRA 405
            ML  IGLGL DE D+++RGL+A+K  D V++E YTS+L        +  LE  YGK I    RE VE  ADKIL  ++  N  FL  GD   ATTH+DL +RA
Sbjct:    1 MLTFIGLGLFDEYDVSVRGLDAIKSADTVFLEVYTSVLMGA----PIERLEAFYGKKITPLYREDVEIHADKILDAAEFGNAVFLTAGDSMVATTHSDLRIRA 99

HSP 2 Score: 30.4166 bits (67), Expect = 5.196e-20
Identity = 15/30 (50.00%), Postives = 20/30 (66.67%), Query Frame = 2 
Query:  419 IQVKAVHNASVMNAV-GICGLQLYRFGETV 505
            I    +H AS+  AV G+ GLQ YRFG++V
Sbjct:  104 IPTTIIHGASITTAVCGLSGLQNYRFGKSV 133
The following BLAST results are available for this feature:
BLAST of EY727817 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 51
Match NameE-valueIdentityDescription
DPHB_METMP6.309e-2545.45Probable diphthine synthase OS=Methanococcus marip... [more]
DPHB_METM72.355e-2445.79Probable diphthine synthase OS=Methanococcus marip... [more]
DPHB_METM52.355e-2443.93Probable diphthine synthase OS=Methanococcus marip... [more]
DPHB_METM61.489e-2342.99Probable diphthine synthase OS=Methanococcus marip... [more]
DPHB_PYRHO3.278e-2350.45Probable diphthine synthase OS=Pyrococcus horikosh... [more]
DPHB_METVS1.224e-2242.99Probable diphthine synthase OS=Methanococcus vanni... [more]
DPHB_UNCMA3.507e-2247.22Probable diphthine synthase OS=Uncultured methanog... [more]
DPHB_PYRFU6.350e-2252.73Probable diphthine synthase OS=Pyrococcus furiosus... [more]
DPHB_METTH8.236e-2144.86Probable diphthine synthase OS=Methanobacterium th... [more]
DPHB_METLZ5.196e-2048.54Probable diphthine synthase OS=Methanocorpusculum ... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-703-106-A10-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY727817 ID=EY727817; Name=EY727817; organism=Citrus sinensis; type=EST; length=987bp
CGCACACTGTTCCTTTTAGGGTTTAAAGGTGTTTTGCTGATCGAAACCAA
AGGAGCTAGGGTTAGAATTTAACAGAGACAGACAGATCACGAAAAGATGC
TATACATAATAGGTCTGGGATTGGGTGACGAGAGAGACATAACGCTGCGA
GGATTAGAGGCCGTGAAGAAATGCGATAAAGTATACATTGAAGCCTACAC
TTCTCTTCTCTCTTTTGGTCTATCAACAGATGGCCTCTCCACTCTGGAAA
AGTTGTATGGGAAACCGATTGCACTTGCTGATAGAGAAATGGTTGAAGAG
AAAGCTGACAAAATTCTCTCTGAATCTCAAGAATCTAACGTGGCTTTTCT
TGTCGTAGGTGACCCTTTCGGAGCTACAACACATACCGATCTGGTTGTTC
GAGCTAAGAAATTGGGGGATTCAAGTGAAGGCAGTACATAATGCATCGGT
GATGAATGCAGTTGGAATTTGTGGGTTACAACTCTACCGCTTTGGAGAGA
CAGTTTTCAATTCCCATTTTTTACTGAAACTTGGAGACCTGGGAACTTTT
TATGAAAAAAATTAAAAAAAAATCCTTTCGCCTTGGGACTGCCACCACTC
CGGCTTGGTAAAAATAAAAAATAAAAAGGGAACCATCTTTGGAAACCTTG
GGGGCAAAGGAAAAAACCTTAATTGAAACCCCCTAAAACCTTGAAAAGGG
AACATTTGGTTATTGGGGCACCTTTTTGGGAAGGGGTGGACTACGGGAAG
GGAAAAATCTGTAACCCAAAGGAAAAACCCTCTTTGGGGGGGGGGTTTGG
CCCGGGTTGGGGAAGGGGGGGAACAAAAAAAAAATTGCCCCGGGTAAAGG
GGAGGGCTCCTCTCCAAGGGGGGGGATATTTTGGGGCCCCCCCCTTTTGC
TTTTTCGCCCTAGGGGGAAATCGTGGGGGGGAGAAACCCCCCCCCCCCAA
TAAAAAAAAAAAAAAAAAAATGGGTTGTGTGTGTTTT
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