EY753356 | Citrus Genome Database

Overview


Name	EY753356
Unique Name	EY753356
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	853

Libraries


Library Name	Type
Sweet orange leaf, field plant A	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY753356 vs. ExPASy Swiss-Prot
Match: GLYA_GEOBB (Serine hydroxymethyltransferase OS=Geobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622) GN=glyA PE=3 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 6.867e-36
Identity = 75/165 (45.45%), Postives = 103/165 (62.42%), Query Frame = 2

Query:  242 LEVVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYLDYDPLEKSA 736
            LE  DP +A++I  E  RQ   LELI SENF S +V++A GSV+TNKY+EGYPG RYYGG   +D+ E+L   RA E F  D     VNVQ  SGS +N  VY ++LKP D ++ ++L HGGHL+HG       ++     F  +PY +++ T  +DY+  E+ A
Sbjct:    4 LETFDPAVAEVIRQETERQEYNLELIASENFVSPAVLEAQGSVLTNKYAEGYPGKRYYGGCHCVDVVENLAIDRAKELFGAD----HVNVQPHSGSQANMAVYFSVLKPGDTVLGMNLAHGGHLTHG-----SPVNFSGKLFNIVPYGVSKETQTIDYEETERLA 159

HSP 2 Score: 30.4166 bits (67), Expect = 6.867e-36
Identity = 12/31 (38.71%), Postives = 20/31 (64.52%), Query Frame = 3

Query:  747 RPKLIGAGARAYARLYDYERIPKVCNKQKAI 839
            +PK+I  GA AY R+ D+E   ++ +K  A+
Sbjct:  163 KPKMIVVGASAYPRIIDFEAFRRIADKVGAV 193

[Close]

BLAST of EY753356 vs. ExPASy Swiss-Prot
Match: GLYA_CLOAB (Serine hydroxymethyltransferase OS=Clostridium acetobutylicum GN=glyA PE=3 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 6.868e-36
Identity = 75/172 (43.60%), Postives = 105/172 (61.05%), Query Frame = 2

Query:  242 LEVVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYLDYDPLEKSATLLQAKI 757
            ++V D E+  IIE E ARQ   +ELI SENFTS +VM+A+GS +TNKY+EGYPG RYYGG   +D  E L ++RA + F+ +      NVQ  SGS +N  VY A+LKP D IM ++L  GGHL+HG       ++     F  + Y +++ T  +DY+   K A   + K+
Sbjct:    6 IKVSDSEVYSIIEEENARQENNIELIASENFTSKAVMEAMGSYLTNKYAEGYPGKRYYGGCYVVDKVEELARERAKKLFKAE----HANVQPHSGSQANMAVYFAVLKPGDTIMGMNLTDGGHLTHG-----SPVNFSGKLFNIIAYGVSDETEQIDYEAFRKKALECKPKM 168

HSP 2 Score: 33.113 bits (74), Expect = 6.868e-36
Identity = 11/27 (40.74%), Postives = 23/27 (85.19%), Query Frame = 3

Query:  747 RPKLIGAGARAYARLYDYERIPKVCNK 827
            +PK+I +GA AY+R+ D+++I ++C++
Sbjct:  165 KPKMIVSGASAYSRIIDFKKIREICDE 191

[Close]

BLAST of EY753356 vs. ExPASy Swiss-Prot
Match: GLYA_PORGI (Serine hydroxymethyltransferase OS=Porphyromonas gingivalis GN=glyA PE=3 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 8.942e-36
Identity = 77/161 (47.83%), Postives = 99/161 (61.49%), Query Frame = 2

Query:  254 DPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYLDYDPLEKSA 736
            D  I D+IE E  RQ KG+ELI SENF S  VMQA+GS MTNKY+EGYPG RYYGG E +D +E +   R  + +  +   W  NVQ  SG+ +N  V  A L+  D  M L+L HGGHLSHG       +++  I +  + Y L+E TG +DYD +EK A
Sbjct:    4 DSVIFDLIEKEHQRQLKGIELIASENFVSEQVMQAMGSCMTNKYAEGYPGKRYYGGCEVVDQSEQIAIDRIKQLYGAE---W-ANVQPHSGAQANMAVLLACLEAGDTFMGLNLEHGGHLSHG-----SLVNSSGILYRPIGYNLSEETGMVDYDHMEKMA 155

HSP 2 Score: 31.187 bits (69), Expect = 8.942e-36
Identity = 13/31 (41.94%), Postives = 22/31 (70.97%), Query Frame = 3

Query:  747 RPKLIGAGARAYARLYDYERIPKVCNKQKAI 839
            +PKLI  G  AY+R +DY+R+ ++ +K  A+
Sbjct:  159 KPKLIIGGGSAYSREWDYKRMREIADKVGAL 189

[Close]

BLAST of EY753356 vs. ExPASy Swiss-Prot
Match: GLYA_PORG3 (Serine hydroxymethyltransferase OS=Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / JCM 12257) GN=glyA PE=3 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 8.942e-36
Identity = 77/161 (47.83%), Postives = 99/161 (61.49%), Query Frame = 2

Query:  254 DPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYLDYDPLEKSA 736
            D  I D+IE E  RQ KG+ELI SENF S  VMQA+GS MTNKY+EGYPG RYYGG E +D +E +   R  + +  +   W  NVQ  SG+ +N  V  A L+  D  M L+L HGGHLSHG       +++  I +  + Y L+E TG +DYD +EK A
Sbjct:    4 DSVIFDLIEKEHQRQLKGIELIASENFVSEQVMQAMGSCMTNKYAEGYPGKRYYGGCEVVDQSEQIAIDRIKQLYGAE---W-ANVQPHSGAQANMAVLLACLEAGDTFMGLNLEHGGHLSHG-----SLVNSSGILYRPIGYNLSEETGMVDYDHMEKMA 155

HSP 2 Score: 31.187 bits (69), Expect = 8.942e-36
Identity = 13/31 (41.94%), Postives = 22/31 (70.97%), Query Frame = 3

Query:  747 RPKLIGAGARAYARLYDYERIPKVCNKQKAI 839
            +PKLI  G  AY+R +DY+R+ ++ +K  A+
Sbjct:  159 KPKLIIGGGSAYSREWDYKRMREIADKVGAL 189

[Close]

BLAST of EY753356 vs. ExPASy Swiss-Prot
Match: GLYA_CLOBK (Serine hydroxymethyltransferase OS=Clostridium botulinum (strain Okra / Type B1) GN=glyA PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 8.945e-36
Identity = 72/165 (43.64%), Postives = 106/165 (64.24%), Query Frame = 2

Query:  242 LEVVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYLDYDPLEKSA 736
            L+  DPE+ D+I+ E+ RQ   +ELI SENFTS+SVM+A+GS++TNKY+EGYP  RYYGG E++D  E L ++R  + F  +      NVQ  SGS +N  VY ++L+  D I+ +DL HGGHL+HG       ++     +  + Y +++ T  +DYD L+K A
Sbjct:    6 LKNTDPELLDMIKKEEERQEYNIELIASENFTSLSVMEAMGSLLTNKYAEGYPHKRYYGGCEFVDEVEDLARERLKKLFVAE----HANVQPHSGSQANMAVYMSVLQTGDTILGMDLSHGGHLTHG-----SPVNFSGKLYNFISYGVDKETETIDYDQLKKIA 161

HSP 2 Score: 35.039 bits (79), Expect = 8.945e-36
Identity = 13/27 (48.15%), Postives = 22/27 (81.48%), Query Frame = 3

Query:  747 RPKLIGAGARAYARLYDYERIPKVCNK 827
            RPK+I +GA AY R+ D+E+I ++C++
Sbjct:  165 RPKMIVSGASAYPRIIDFEKIREICDE 191

[Close]

BLAST of EY753356 vs. ExPASy Swiss-Prot
Match: GLYA_CLOK5 (Serine hydroxymethyltransferase OS=Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) GN=glyA PE=3 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 8.946e-36
Identity = 77/165 (46.67%), Postives = 101/165 (61.21%), Query Frame = 2

Query:  242 LEVVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYLDYDPLEKSA 736
            L+  D +I  IIE E  RQ  G+ELI SENFTS SVM+A+GS +TNKY+EGYPG RYYGG   +D AE L + R  + F  +     VNVQ  SGS +N  VY ++LKP D ++ + L HGGHL+HG      K+S     +  + Y LN  T  +DYD + + A
Sbjct:    6 LKNTDKDIYGIIEEEWERQKNGIELIASENFTSKSVMEAMGSFLTNKYAEGYPGKRYYGGCYIVDKAEDLARDRMKKLFNAE----HVNVQPHSGSQANMAVYMSVLKPGDTVLGMSLNHGGHLTHG-----SKVSFSGKLYNFVSYGLNSDTEIIDYDEMRELA 161

HSP 2 Score: 30.8018 bits (68), Expect = 8.946e-36
Identity = 11/27 (40.74%), Postives = 21/27 (77.78%), Query Frame = 3

Query:  747 RPKLIGAGARAYARLYDYERIPKVCNK 827
            +PK+I +GA AY R  D+++I ++C++
Sbjct:  165 KPKMIVSGASAYPRKIDFKKIREICDE 191

[Close]

BLAST of EY753356 vs. ExPASy Swiss-Prot
Match: GLYA_CLOK1 (Serine hydroxymethyltransferase OS=Clostridium kluyveri (strain NBRC 12016) GN=glyA PE=3 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 8.946e-36
Identity = 77/165 (46.67%), Postives = 101/165 (61.21%), Query Frame = 2

Query:  242 LEVVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYLDYDPLEKSA 736
            L+  D +I  IIE E  RQ  G+ELI SENFTS SVM+A+GS +TNKY+EGYPG RYYGG   +D AE L + R  + F  +     VNVQ  SGS +N  VY ++LKP D ++ + L HGGHL+HG      K+S     +  + Y LN  T  +DYD + + A
Sbjct:    6 LKNTDKDIYGIIEEEWERQKNGIELIASENFTSKSVMEAMGSFLTNKYAEGYPGKRYYGGCYIVDKAEDLARDRMKKLFNAE----HVNVQPHSGSQANMAVYMSVLKPGDTVLGMSLNHGGHLTHG-----SKVSFSGKLYNFVSYGLNSDTEIIDYDEMRELA 161

HSP 2 Score: 30.8018 bits (68), Expect = 8.946e-36
Identity = 11/27 (40.74%), Postives = 21/27 (77.78%), Query Frame = 3

Query:  747 RPKLIGAGARAYARLYDYERIPKVCNK 827
            +PK+I +GA AY R  D+++I ++C++
Sbjct:  165 KPKMIVSGASAYPRKIDFKKIREICDE 191

[Close]

BLAST of EY753356 vs. ExPASy Swiss-Prot
Match: GLYA_PROA2 (Serine hydroxymethyltransferase OS=Prosthecochloris aestuarii (strain DSM 271 / SK 413) GN=glyA PE=3 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 1.516e-35
Identity = 81/175 (46.29%), Postives = 108/175 (61.71%), Query Frame = 2

Query:  242 LEVVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYLDYDPLEKSATLLQAKIDWC 766
            L+  D EI D I +E  RQ + LELI SENF S +VM+A GSVMTNKY+EGYPG RYYGG E++D+AE L ++RA + F        VNVQ  SGS +N  V  ++LKP D IM LDL HGGHL+HG       ++     F+   Y ++  TG +D D +E  A  ++ ++  C
Sbjct:    6 LQKQDKEIFDAISNEVLRQTETLELIASENFASKAVMEACGSVMTNKYAEGYPGKRYYGGCEFVDVAEDLARERAKKLFGCQ----YVNVQPHSGSSANMGVLFSVLKPGDTIMGLDLSHGGHLTHG-----SSVNFSGQLFDAHSYGVDRETGLIDMDRVEDMALSVRPRLIIC 171

HSP 2 Score: 26.5646 bits (57), Expect = 1.516e-35
Identity = 12/34 (35.29%), Postives = 21/34 (61.76%), Query Frame = 3

Query:  747 RPKLIGAGARAYARLYDYERIPKVCNKQKAIRSA 848
            RP+LI  GA AY++ +D++   ++ +K  A   A
Sbjct:  165 RPRLIICGASAYSQGFDFKAFRQIADKVGAFLMA 198

[Close]

BLAST of EY753356 vs. ExPASy Swiss-Prot
Match: GLYA_SYNFM (Serine hydroxymethyltransferase OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) GN=glyA PE=3 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 1.518e-35
Identity = 77/165 (46.67%), Postives = 106/165 (64.24%), Query Frame = 2

Query:  242 LEVVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYLDYDPLEKSA 736
            LE +DPEIAD+I  E+ RQ   LELI SENF S +V +A GSV+TNKY+EGYPG RYYGG E++DMAE L Q+RA + F  D E    NVQ  SGS +N  ++ A+L+P D ++ +DL  GGHL+HG       +S     +  + Y + + T  +D+D + + A
Sbjct:    4 LERIDPEIADVICEEEKRQRGKLELIASENFVSEAVREAQGSVLTNKYAEGYPGKRYYGGCEFVDMAERLAQERAKKLF--DAEY--ANVQPHSGSQANMAIFFAVLQPGDTVLGMDLRQGGHLTHG-----SPVSFSGKLYNVVSYGVRKDTEQIDFDQVARLA 159

HSP 2 Score: 30.0314 bits (66), Expect = 1.518e-35
Identity = 13/27 (48.15%), Postives = 18/27 (66.67%), Query Frame = 3

Query:  747 RPKLIGAGARAYARLYDYERIPKVCNK 827
            RPKLI AGA AY R+ D+ R  ++  +
Sbjct:  163 RPKLIIAGASAYPRIIDFARFGQIAKE 189

[Close]

BLAST of EY753356 vs. ExPASy Swiss-Prot
Match: GLYA_FLAJ1 (Serine hydroxymethyltransferase OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) GN=glyA PE=3 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 1.976e-35
Identity = 79/168 (47.02%), Postives = 102/168 (60.71%), Query Frame = 2

Query:  254 DPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLNESTGYLDYDPLEKSATLLQAKI 757
            D +I D+I+ EK RQ  GLELI SENF S  VM+A GSV+TNKY+EGYPG RYYGG E +D+ E +   RA E F  +      NVQ  SGS +N  VY A L P D I+  DL HGGHL+HG       ++     +  + Y ++  TG LDYD +++ AT  Q K+
Sbjct:    4 DEQIFDLIQEEKDRQIHGLELIASENFVSDEVMEAAGSVLTNKYAEGYPGKRYYGGCEVVDVIEQIAIDRAKELFGAE----YANVQPHSGSQANTAVYHACLNPGDTILGFDLSHGGHLTHG-----SPVNFSGRLYRPVFYGVDAETGRLDYDKIQEIATKEQPKL 162

HSP 2 Score: 28.4906 bits (62), Expect = 1.976e-35
Identity = 14/31 (45.16%), Postives = 20/31 (64.52%), Query Frame = 3

Query:  747 RPKLIGAGARAYARLYDYERIPKVCNKQKAI 839
            +PKLI AGA AY+R  D+ R  ++ +   AI
Sbjct:  159 QPKLIIAGASAYSRDMDFARFRQIADSVGAI 189

The following BLAST results are available for this feature:

BLAST of EY753356 vs. ExPASy Swiss-Prot

BLAST of EY753356 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
GLYA_GEOBB	6.867e-36	45.45	Serine hydroxymethyltransferase OS=Geobacter bemid...	[more]
GLYA_CLOAB	6.868e-36	43.60	Serine hydroxymethyltransferase OS=Clostridium ace...	[more]
GLYA_PORGI	8.942e-36	47.83	Serine hydroxymethyltransferase OS=Porphyromonas g...	[more]
GLYA_PORG3	8.942e-36	47.83	Serine hydroxymethyltransferase OS=Porphyromonas g...	[more]
GLYA_CLOBK	8.945e-36	43.64	Serine hydroxymethyltransferase OS=Clostridium bot...	[more]
GLYA_CLOK5	8.946e-36	46.67	Serine hydroxymethyltransferase OS=Clostridium klu...	[more]
GLYA_CLOK1	8.946e-36	46.67	Serine hydroxymethyltransferase OS=Clostridium klu...	[more]
GLYA_PROA2	1.516e-35	46.29	Serine hydroxymethyltransferase OS=Prosthecochlori...	[more]
GLYA_SYNFM	1.518e-35	46.67	Serine hydroxymethyltransferase OS=Syntrophobacter...	[more]
GLYA_FLAJ1	1.976e-35	47.02	Serine hydroxymethyltransferase OS=Flavobacterium ...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS12-C1-001-001-H01-CT.F Sweet orange leaf, field plant A Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY753356 ID=EY753356; Name=EY753356; organism=Citrus sinensis; type=EST; length=853bp
CCACGCGTCCGCATAATCATACAACAAACAAAGAAGAAACAGACAACATC
TTGCAGCGGGAGAGTGAGAAGCAAAAATGGCCATGGCTCTGGCTCTTCGC
AGGCTCTCATCTTCCCTCGACAAACCCACTCGTCCTCTCTTCAATGGCTC
CTCCCTCTACTACATGTCATCTTTGCCAAATGAAGCTGTTTACGAGAAAG
AGAAAAATGGTGTCACTTGGCCAAAACAACTGAATGCTCCACTTGAGGTC
GTTGATCCTGAGATTGCTGACATTATCGAGCATGAAAAAGCACGACAATG
GAAGGGACTTGAACTCATTCCTTCAGAGAACTTTACTTCAGTATCCGTGA
TGCAAGCTGTGGGGTCTGTCATGACCAACAAGTACAGTGAAGGATATCCA
GGTGCAAGATATTATGGAGGAAACGAGTATATTGACATGGCGGAATCCTT
ATGTCAGAAGCGAGCTCTGGAAGCATTTCGGTTGGATCCTGAAAAATGGG
GAGTGAATGTGCAGTCTCTGTCTGGATCTCCATCTAATTTTCAAGTCTAC
ACTGCATTGTTAAAACCTCATGATAGGATCATGGCACTTGATCTTCCTCA
TGGTGGGCATCTCTCACACGGCTATCAGACAGATACCAAAAAGATATCTG
CAGTGTCCATATTTTTTGAGACAATGCCATACAGATTGAATGAGAGCACT
GGCTATCTAGACTATGACCCGTTGGAGAAAAGTGCCACCCTTCTTCAGGC
CAAAATTGATTGGTGCTGGGGCCAGGGCTTATGCACGATTGTACGATTAT
GAGCGTATTCCAAAGGTTTGCAACAAACAAAAAGCCATAAGGTCGGCGTA
ATG