EY720914 | Citrus Genome Database

Overview


Name	EY720914
Unique Name	EY720914
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	879

Libraries


Library Name	Type
Sweet orange fruit, development stadium (4 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of EY720914 vs. ExPASy Swiss-Prot
Match: NEDD8_CAEEL (NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 1.894e-19
Identity = 46/76 (60.53%), Postives = 58/76 (76.32%), Query Frame = 1

Query:   52 MQIFVKTLTGKTITLEVESSDTIDSVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 279
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76

HSP 2 Score: 80.8777 bits (198), Expect = 1.075e-14
Identity = 40/76 (52.63%), Postives = 52/76 (68.42%), Query Frame = 1

Query:  280 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQHNEGIAPDHQRLIFAGEQLEDGRTLADYSLQNESALPLVLRLREG 507
            M I VKTLTGK I L++E +D ++ +K K++  EGI P  QRLIFAG+Q+ D +T ADY +   S L LVL LR G
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76

BLAST of EY720914 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 96.2857 bits (238), Expect = 2.473e-19
Identity = 46/75 (61.33%), Postives = 59/75 (78.67%), Query Frame = 1

Query:   58 IFVKTLTGKTITLEVESSDTIDSVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 282
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

HSP 2 Score: 81.2629 bits (199), Expect = 8.233e-15
Identity = 39/75 (52.00%), Postives = 54/75 (72.00%), Query Frame = 1

Query:  286 IFVKTLTGKTITLEVESSDTIDNVKAKIQHNEGIAPDHQRLIFAGEQLEDGRTLADYSLQNESALPLVLRLREGV 510
            I VKTLTGK I +++E +DTID +K +++  EGI P  QRLI+AG+QL D +T  DY+++  S L LVL LR G+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

BLAST of EY720914 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.734e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:   52 MQIFVKTLTGKTITLEVESSDTIDSVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 279
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 77.0258 bits (188), Expect = 1.553e-13
Identity = 36/76 (47.37%), Postives = 52/76 (68.42%), Query Frame = 1

Query:  280 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQHNEGIAPDHQRLIFAGEQLEDGRTLADYSLQNESALPLVLRLREG 507
            M I VKTLTGK I +++E +D ++ +K +++  EGI P  QRLI++G+Q+ D +T ADY +   S L LVL LR G
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of EY720914 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.734e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:   52 MQIFVKTLTGKTITLEVESSDTIDSVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 279
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 77.0258 bits (188), Expect = 1.553e-13
Identity = 36/76 (47.37%), Postives = 52/76 (68.42%), Query Frame = 1

Query:  280 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQHNEGIAPDHQRLIFAGEQLEDGRTLADYSLQNESALPLVLRLREG 507
            M I VKTLTGK I +++E +D ++ +K +++  EGI P  QRLI++G+Q+ D +T ADY +   S L LVL LR G
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of EY720914 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 92.8189 bits (229), Expect = 2.734e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:   52 MQIFVKTLTGKTITLEVESSDTIDSVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 279
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 77.0258 bits (188), Expect = 1.553e-13
Identity = 36/76 (47.37%), Postives = 52/76 (68.42%), Query Frame = 1

Query:  280 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQHNEGIAPDHQRLIFAGEQLEDGRTLADYSLQNESALPLVLRLREG 507
            M I VKTLTGK I +++E +D ++ +K +++  EGI P  QRLI++G+Q+ D +T ADY +   S L LVL LR G
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of EY720914 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.734e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:   52 MQIFVKTLTGKTITLEVESSDTIDSVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 279
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 77.0258 bits (188), Expect = 1.553e-13
Identity = 36/76 (47.37%), Postives = 52/76 (68.42%), Query Frame = 1

Query:  280 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQHNEGIAPDHQRLIFAGEQLEDGRTLADYSLQNESALPLVLRLREG 507
            M I VKTLTGK I +++E +D ++ +K +++  EGI P  QRLI++G+Q+ D +T ADY +   S L LVL LR G
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of EY720914 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.734e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:   52 MQIFVKTLTGKTITLEVESSDTIDSVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 279
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 77.0258 bits (188), Expect = 1.553e-13
Identity = 36/76 (47.37%), Postives = 52/76 (68.42%), Query Frame = 1

Query:  280 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQHNEGIAPDHQRLIFAGEQLEDGRTLADYSLQNESALPLVLRLREG 507
            M I VKTLTGK I +++E +D ++ +K +++  EGI P  QRLI++G+Q+ D +T ADY +   S L LVL LR G
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of EY720914 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 3.571e-18
Identity = 41/76 (53.95%), Postives = 58/76 (76.32%), Query Frame = 1

Query:   52 MQIFVKTLTGKTITLEVESSDTIDSVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 279
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

HSP 2 Score: 76.6406 bits (187), Expect = 2.028e-13
Identity = 35/76 (46.05%), Postives = 52/76 (68.42%), Query Frame = 1

Query:  280 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQHNEGIAPDHQRLIFAGEQLEDGRTLADYSLQNESALPLVLRLREG 507
            + + V+TLTGK I +++E +DT+D +K +++  EGI P  QRLI+ G+QL D +T  DY ++  S L LVL LR G
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

BLAST of EY720914 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 2.315e-17
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = 1

Query:   52 MQIFVKTLTGKTITLEVESSDTIDSVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 279
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

HSP 2 Score: 77.0258 bits (188), Expect = 1.553e-13
Identity = 38/76 (50.00%), Postives = 52/76 (68.42%), Query Frame = 1

Query:  280 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQHNEGIAPDHQRLIFAGEQLEDGRTLADYSLQNESALPLVLRLREG 507
            M+I VKTLT K I +E+E +DTI+ +K +I+  EGI P HQR+++ G+QL D  T   Y+L+  S L LVL LR G
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

BLAST of EY720914 vs. ExPASy Swiss-Prot
Match: NEDD8_DICDI (NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV=2)

HSP 1 Score: 89.3521 bits (220), Expect = 3.023e-17
Identity = 41/77 (53.25%), Postives = 58/77 (75.32%), Query Frame = 1

Query:   52 MQIFVKTLTGKTITLEVESSDTIDSVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 282
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77

HSP 2 Score: 74.3294 bits (181), Expect = 1.006e-12
Identity = 35/77 (45.45%), Postives = 52/77 (67.53%), Query Frame = 1

Query:  280 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQHNEGIAPDHQRLIFAGEQLEDGRTLADYSLQNESALPLVLRLREGV 510
            M I VKTLTGK I ++++ +D I  +K +++  EGI P  QRLIF G+Q+ D +  ++YS++  S L LVL LR G+
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77

The following BLAST results are available for this feature:

BLAST of EY720914 vs. ExPASy Swiss-Prot

BLAST of EY720914 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 96


Match Name	E-value	Identity	Description
NEDD8_CAEEL	1.894e-19	60.53	NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1	[more]
RUB2_ARATH	2.473e-19	61.33	NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN...	[more]
NEDD8_RAT	2.734e-18	56.58	NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1	[more]
NEDD8_RABIT	2.734e-18	56.58	NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1	[more]
NEDD8_MOUSE	2.734e-18	56.58	NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2	[more]
NEDD8_HUMAN	2.734e-18	56.58	NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1	[more]
NEDD8_BOVIN	2.734e-18	56.58	NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1	[more]
RUB3_ORYSJ	3.571e-18	53.95	NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap...	[more]
RUB3_ARATH	2.315e-17	55.26	NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN...	[more]
NEDD8_DICDI	3.023e-17	53.25	NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-703-016-A02-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY720914 ID=EY720914; Name=EY720914; organism=Citrus sinensis; type=EST; length=879bp
TCGCGACATTATCAATTCTTCCTTCGTTCGCAAGAAAAAGCTCTGTTCGA
TATGCAGATCTTCGTGAAAACCTTGACCGGCAAAACTATTACTCTCGAAG
TCGAGAGCTCGGACACCATCGACAGCGTAAAGGCCAAGATCCAAGACAAG
GAGGGAATTCCGCCGGACCAGCAGAGGCTTATCTTTGCTGGAAAGCAGCT
CGAGGACGGCCGCACCTTGGCCGATTACAACATCCAGAAGGAGTCAACCC
TTCATCTTGTCCTTCGTCTCCGCGGAGGCATGCAGATCTTCGTGAAAACC
CTGACTGGCAAAACTATTACTCTCGAAGTCGAGAGCTCCGACACCATCGA
CAACGTCAAGGCGAAGATTCAACACAATGAGGGAATCGCCCCGGACCATC
AAAGGCTTATCTTCGCTGGAGAGCAGCTCGAGGACGGCCGCACCTTGGCC
GATTATAGCCTACAGAACGAGTCTGCCCTCCCTCTTGTCCTTCGTCTCCG
CGAGGGCGTGGAGATTGTGGGGAAACCTGACGGGGAGGAGGTGGAGCTGG
TGGGGGAGAGGTGGACCGGAGGGGAAGGCGGGGCGGGATCGATAGAGGGC
GGGGTGCGGGGGGGGGGGAGGAGGGGGAGGTAGGGAGGAGAGGGGGGGGG
GGGGCGCGCCGGGGCGGCGGCGGGTTGTTGGGGGGGGTGTGGTGGGGGGG
TGGAGGGGGGGGGGGGGGGGGGGAGGGGGGGAGAAGAGTGAGGGGGGGGA
GGAGGGGGGGCGTGCAGACAGAGAGAGAGCTGGAGTTGGAAGGTGCGGGC
CGGGGGGGAGGGGGAGGGGGTGGGCGGGGAGAGAGTGGGGGGGCGTAGTT
GGAGAACGGAGTGGAGTGCGGGGGGAGCG