EY720144 | Citrus Genome Database

Overview


Name	EY720144
Unique Name	EY720144
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	853

Libraries


Library Name	Type
Sweet orange fruit, development stadium (4 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY720144 vs. ExPASy Swiss-Prot
Match: QUEC_LEGPA (Queuosine biosynthesis protein queC OS=Legionella pneumophila (strain Paris) GN=queC PE=3 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 7.440e-13
Identity = 24/58 (41.38%), Postives = 36/58 (62.07%), Query Frame = 2

Query:  308 KRAVILLSGGLDSATVVAMARADGYSCYTMSFDYGQXXXXXXXXXXXXXXDLGVVEHR 481
            K+AV+LLSGGLDS T +A+A++ G++CY +SF YGQ               +G  +H+
Sbjct:    2 KKAVVLLSGGLDSTTCLALAKSQGFACYALSFSYGQRHSAELCAATRIAKHMGAADHK 59

HSP 2 Score: 30.0314 bits (66), Expect = 7.440e-13
Identity = 19/44 (43.18%), Postives = 27/44 (61.36%), Query Frame = 1

Query:  478 QVIGLNLDGMGGSALTDSSIDVPQ-GPSEGIPGD*VAARYHVLL 606
            +++ L+    GGSALTD+SI+VP+   S  IP   V AR  + L
Sbjct:   59 KIVTLDTALFGGSALTDASIEVPEFKESPEIPVTYVPARNTIFL 102

HSP 3 Score: 27.7202 bits (60), Expect = 7.440e-13
Identity = 12/29 (41.38%), Postives = 17/29 (58.62%), Query Frame = 3

Query:  678 YAGYPDCRPYYLESCERGATSPPWAVLNG 764
            Y+ YPDCRP ++ES +  A     A + G
Sbjct:  126 YSHYPDCRPEFIESFQSLANLATKAGIEG 154

[Close]

BLAST of EY720144 vs. ExPASy Swiss-Prot
Match: QUEC_CLOTH (Queuosine biosynthesis protein queC OS=Clostridium thermocellum (strain ATCC 27405 / DSM 1237) GN=queC PE=3 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 7.456e-13
Identity = 23/36 (63.89%), Postives = 30/36 (83.33%), Query Frame = 2

Query:  308 KRAVILLSGGLDSATVVAMARADGYSCYTMSFDYGQ 415
            +RAV+LLSGGLDS T +++A A+GY  Y +SFDYGQ
Sbjct:    2 RRAVVLLSGGLDSTTCLSVALAEGYEVYPLSFDYGQ 37

HSP 2 Score: 33.8834 bits (76), Expect = 7.456e-13
Identity = 13/35 (37.14%), Postives = 23/35 (65.71%), Query Frame = 3

Query:  663 VTALYYAGYPDCRPYYLESCERGATSPPWAVLNGR 767
            V A+ Y+GYPDCRP ++E+ ++       + +NG+
Sbjct:  119 VNAVDYSGYPDCRPEFIEAFQKAINLGTKSGVNGK 153

HSP 3 Score: 27.7202 bits (60), Expect = 7.456e-13
Identity = 13/27 (48.15%), Postives = 19/27 (70.37%), Query Frame = 1

Query:  490 LNLDGMGGSALTDSSIDVPQGPSEGIP 570
            + +  +GGSALTD +IDVP    +G+P
Sbjct:   61 VKIGNVGGSALTDMNIDVPD--YKGLP 85

[Close]

BLAST of EY720144 vs. ExPASy Swiss-Prot
Match: QUEC_DESHD (Queuosine biosynthesis protein queC OS=Desulfitobacterium hafniense (strain DCB-2 / DSM 10664) GN=queC PE=3 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 9.601e-13
Identity = 22/36 (61.11%), Postives = 28/36 (77.78%), Query Frame = 2

Query:  308 KRAVILLSGGLDSATVVAMARADGYSCYTMSFDYGQ 415
            K+AV+LLSGGLDS T +++A   GY  Y +SFDYGQ
Sbjct:    2 KKAVVLLSGGLDSTTCMSVAHKAGYELYPLSFDYGQ 37

HSP 2 Score: 32.3426 bits (72), Expect = 9.601e-13
Identity = 12/38 (31.58%), Postives = 26/38 (68.42%), Query Frame = 3

Query:  663 VTALYYAGYPDCRPYYLESCERGATSPPWAVLNGRVLA 776
            ++++ Y+GYPDCRP +L++ ++       A ++G+ +A
Sbjct:  119 ISSVDYSGYPDCRPEFLQAFQKVVDVGTKAGVSGQTIA 156

HSP 3 Score: 31.5722 bits (70), Expect = 9.601e-13
Identity = 20/47 (42.55%), Postives = 27/47 (57.45%), Query Frame = 1

Query:  490 LNLDGMGGSALTDSSIDVPQGPSEG-IPGD*VAARYHVLLYPGPGLG 627
            + ++ +GGSALTD+SI VP    +G IP   V AR  + L    G G
Sbjct:   61 IKIEHVGGSALTDASIQVPDYTEDGQIPVTYVPARNILFLSYALGYG 107

[Close]

BLAST of EY720144 vs. ExPASy Swiss-Prot
Match: QUEC2_DESHY (Queuosine biosynthesis protein queC 2 OS=Desulfitobacterium hafniense (strain Y51) GN=queC2 PE=3 SV=2)

HSP 1 Score: 50.0618 bits (118), Expect = 9.601e-13
Identity = 22/36 (61.11%), Postives = 28/36 (77.78%), Query Frame = 2

Query:  308 KRAVILLSGGLDSATVVAMARADGYSCYTMSFDYGQ 415
            K+AV+LLSGGLDS T +++A   GY  Y +SFDYGQ
Sbjct:    2 KKAVVLLSGGLDSTTCMSVAHKAGYELYPLSFDYGQ 37

HSP 2 Score: 32.3426 bits (72), Expect = 9.601e-13
Identity = 12/38 (31.58%), Postives = 26/38 (68.42%), Query Frame = 3

Query:  663 VTALYYAGYPDCRPYYLESCERGATSPPWAVLNGRVLA 776
            ++++ Y+GYPDCRP +L++ ++       A ++G+ +A
Sbjct:  119 ISSVDYSGYPDCRPEFLQAFQKVVDVGTKAGVSGQTIA 156

HSP 3 Score: 31.5722 bits (70), Expect = 9.601e-13
Identity = 20/47 (42.55%), Postives = 27/47 (57.45%), Query Frame = 1

Query:  490 LNLDGMGGSALTDSSIDVPQGPSEG-IPGD*VAARYHVLLYPGPGLG 627
            + ++ +GGSALTD+SI VP    +G IP   V AR  + L    G G
Sbjct:   61 IKIEHVGGSALTDASIQVPDYTEDGQIPVTYVPARNILFLSYALGYG 107

[Close]

BLAST of EY720144 vs. ExPASy Swiss-Prot
Match: QUEC_CYAP7 (Queuosine biosynthesis protein queC OS=Cyanothece sp. (strain PCC 7424) GN=queC PE=3 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 9.610e-13
Identity = 27/59 (45.76%), Postives = 32/59 (54.24%), Query Frame = 2

Query:  302 DQKRAVILLSGGLDSATVVAMARADGYSCYTMSFDYGQXXXXXXXXXXXXXXDLGVVEH 478
            +Q +AVILLSGGLDSAT  A+A A GY    +S  YGQ               LG+ EH
Sbjct:    2 NQPKAVILLSGGLDSATTAAIALASGYEAIALSLFYGQRHHKEIEAAQKIVQVLGIKEH 60

HSP 2 Score: 34.6538 bits (78), Expect = 9.610e-13
Identity = 12/22 (54.55%), Postives = 18/22 (81.82%), Query Frame = 3

Query:  663 VTALYYAGYPDCRPYYLESCER 728
            + A+ Y+GYPDCRP YLE+ ++
Sbjct:  124 INAIDYSGYPDCRPEYLEAFQK 145

HSP 3 Score: 29.6462 bits (65), Expect = 9.610e-13
Identity = 13/20 (65.00%), Postives = 16/20 (80.00%), Query Frame = 1

Query:  490 LNLDGMGGSALTDSSIDVPQ 549
            +NL   GGSALTD SI+VP+
Sbjct:   65 VNLSQWGGSALTDQSIEVPK 84

[Close]

BLAST of EY720144 vs. ExPASy Swiss-Prot
Match: QUEC_CYAP4 (Queuosine biosynthesis protein queC OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) GN=queC PE=3 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 9.620e-13
Identity = 25/57 (43.86%), Postives = 35/57 (61.40%), Query Frame = 2

Query:  311 RAVILLSGGLDSATVVAMARADGYSCYTMSFDYGQXXXXXXXXXXXXXXDLGVVEHR 481
            + VILLSGGLDS TV+ +AR+ G+ CY +SF+YGQ                GV++H+
Sbjct:    2 KIVILLSGGLDSTTVLYLARSLGHDCYALSFNYGQRHKQELAAAIAVAQLAGVIQHQ 58

HSP 2 Score: 31.5722 bits (70), Expect = 9.620e-13
Identity = 21/48 (43.75%), Postives = 27/48 (56.25%), Query Frame = 1

Query:  478 QVIGLNLDGMGGSALTDSSIDVPQG-----PSEGIPGD*VAARYHVLL 606
            QVI  +L   GGSALTD ++D+PQ       +E IP   V AR  + L
Sbjct:   58 QVISFDLGLWGGSALTDYTLDLPQNRAFKEMAEQIPITYVPARNTIFL 105

HSP 3 Score: 30.4166 bits (67), Expect = 9.620e-13
Identity = 10/23 (43.48%), Postives = 17/23 (73.91%), Query Frame = 3

Query:  657 SAVTALYYAGYPDCRPYYLESCE 725
            + + A+ Y+GYPDCRP Y+ + +
Sbjct:  122 AGMNAIDYSGYPDCRPDYIAAMQ 144

[Close]

BLAST of EY720144 vs. ExPASy Swiss-Prot
Match: QUEC_CLOK5 (Queuosine biosynthesis protein queC OS=Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) GN=queC PE=3 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 1.245e-12
Identity = 24/36 (66.67%), Postives = 30/36 (83.33%), Query Frame = 2

Query:  308 KRAVILLSGGLDSATVVAMARADGYSCYTMSFDYGQ 415
            K+AV+LLSGGLDS TV+ +A++ GY  Y MSFDYGQ
Sbjct:    2 KKAVVLLSGGLDSTTVLYLAKSQGYEVYAMSFDYGQ 37

HSP 2 Score: 33.113 bits (74), Expect = 1.245e-12
Identity = 20/44 (45.45%), Postives = 27/44 (61.36%), Query Frame = 1

Query:  481 VIGLNLDGMGGSALTDSSIDVPQ--GPSEGIPGD*VAARYHVLL 606
            ++  N++  GGSALTD+SI VP+    SE IP   V AR  + L
Sbjct:   60 LVTTNMNAWGGSALTDNSIKVPEFNEESEKIPVTYVPARNMIFL 103

HSP 3 Score: 25.409 bits (54), Expect = 1.245e-12
Identity = 9/22 (40.91%), Postives = 16/22 (72.73%), Query Frame = 3

Query:  663 VTALYYAGYPDCRPYYLESCER 728
            V+ + Y+GY DCR  +++S E+
Sbjct:  122 VSEVDYSGYVDCRHEFIDSMEK 143

[Close]

BLAST of EY720144 vs. ExPASy Swiss-Prot
Match: QUEC_CLOK1 (Queuosine biosynthesis protein queC OS=Clostridium kluyveri (strain NBRC 12016) GN=queC PE=3 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 1.245e-12
Identity = 24/36 (66.67%), Postives = 30/36 (83.33%), Query Frame = 2

Query:  308 KRAVILLSGGLDSATVVAMARADGYSCYTMSFDYGQ 415
            K+AV+LLSGGLDS TV+ +A++ GY  Y MSFDYGQ
Sbjct:    2 KKAVVLLSGGLDSTTVLYLAKSQGYEVYAMSFDYGQ 37

HSP 2 Score: 33.113 bits (74), Expect = 1.245e-12
Identity = 20/44 (45.45%), Postives = 27/44 (61.36%), Query Frame = 1

Query:  481 VIGLNLDGMGGSALTDSSIDVPQ--GPSEGIPGD*VAARYHVLL 606
            ++  N++  GGSALTD+SI VP+    SE IP   V AR  + L
Sbjct:   60 LVTTNMNAWGGSALTDNSIKVPEFNEESEKIPVTYVPARNMIFL 103

HSP 3 Score: 25.409 bits (54), Expect = 1.245e-12
Identity = 9/22 (40.91%), Postives = 16/22 (72.73%), Query Frame = 3

Query:  663 VTALYYAGYPDCRPYYLESCER 728
            V+ + Y+GY DCR  +++S E+
Sbjct:  122 VSEVDYSGYVDCRHEFIDSMEK 143

[Close]

BLAST of EY720144 vs. ExPASy Swiss-Prot
Match: QUEC_SYNSC (Queuosine biosynthesis protein queC OS=Synechococcus sp. (strain CC9605) GN=queC PE=3 SV=1)

HSP 1 Score: 46.9802 bits (110), Expect = 1.610e-12
Identity = 25/58 (43.10%), Postives = 30/58 (51.72%), Query Frame = 2

Query:  305 QKRAVILLSGGLDSATVVAMARADGYSCYTMSFDYGQXXXXXXXXXXXXXXDLGVVEH 478
            Q+ A+ LLSGGLDSAT  A+A   G     +SFDYGQ               LG+ EH
Sbjct:    3 QRTAIALLSGGLDSATAAALALEQGDRVIGLSFDYGQRHLRELDAAAAVAQQLGLAEH 60

HSP 2 Score: 34.6538 bits (78), Expect = 1.610e-12
Identity = 26/56 (46.43%), Postives = 31/56 (55.36%), Query Frame = 1

Query:  484 IGLNLDGMGGSALTDSSIDVP-QGPSEG-IPGD*VAARYHVLLYPGPGLGPNSRGA 645
            I +NL   GGSALTD+SI +P  G  EG IP   V  R  V +  G  L   +RGA
Sbjct:   63 ISVNLASWGGSALTDASISIPTDGVEEGRIPPTYVPGRNTVFISVGLSLA-EARGA 117

HSP 3 Score: 31.5722 bits (70), Expect = 1.610e-12
Identity = 12/19 (63.16%), Postives = 15/19 (78.95%), Query Frame = 3

Query:  663 VTALYYAGYPDCRPYYLES 719
            V A+ Y+GYPDCRP YL +
Sbjct:  124 VNAVDYSGYPDCRPDYLNA 142

[Close]

BLAST of EY720144 vs. ExPASy Swiss-Prot
Match: QUEC_NITEU (Queuosine biosynthesis protein queC OS=Nitrosomonas europaea GN=queC PE=3 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 2.361e-12
Identity = 40/122 (32.79%), Postives = 58/122 (47.54%), Query Frame = 2

Query:  308 KRAVILLSGGLDSATVVAMARADGYSCYTMSFDYGQXXXXXXXXXXXXXXDLGVVEHR----*SG*TSTAWVVRR*PTAP*TCRKAPAKGSRVTKWLRGTTSCCTLALGWGRILEARHIFVG 661
            K+AV+LLSGG+DSAT +A+AR  G++CY +S DYGQ               L V +H+         +++ +     T P       + G  VT      T    LAL W  +L +  IF+G
Sbjct:    2 KKAVVLLSGGMDSATTLAIARQSGFACYALSIDYGQRHVAELAAAARIGQSLQVSDHQFLKLDLAVLASSVLTDISATVP---LHGTSTGIPVTYVPARNTIMLALALAWAEVLGSHDIFIG 120

HSP 2 Score: 34.2686 bits (77), Expect = 2.361e-12
Identity = 17/37 (45.95%), Postives = 23/37 (62.16%), Query Frame = 3

Query:  663 VTALYYAGYPDCRPYYLESCERGATSPPWAVLNGRVL 773
            VTA+ Y+GYPDCR  Y+++ E+ A     A   G VL
Sbjct:  121 VTAVDYSGYPDCRRDYIDAFEKMANLATKAGREGMVL 157

The following BLAST results are available for this feature:

BLAST of EY720144 vs. ExPASy Swiss-Prot

BLAST of EY720144 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 61


Match Name	E-value	Identity	Description
QUEC_LEGPA	7.440e-13	41.38	Queuosine biosynthesis protein queC OS=Legionella ...	[more]
QUEC_CLOTH	7.456e-13	63.89	Queuosine biosynthesis protein queC OS=Clostridium...	[more]
QUEC_DESHD	9.601e-13	61.11	Queuosine biosynthesis protein queC OS=Desulfitoba...	[more]
QUEC2_DESHY	9.601e-13	61.11	Queuosine biosynthesis protein queC 2 OS=Desulfito...	[more]
QUEC_CYAP7	9.610e-13	45.76	Queuosine biosynthesis protein queC OS=Cyanothece ...	[more]
QUEC_CYAP4	9.620e-13	43.86	Queuosine biosynthesis protein queC OS=Cyanothece ...	[more]
QUEC_CLOK5	1.245e-12	66.67	Queuosine biosynthesis protein queC OS=Clostridium...	[more]
QUEC_CLOK1	1.245e-12	66.67	Queuosine biosynthesis protein queC OS=Clostridium...	[more]
QUEC_SYNSC	1.610e-12	43.10	Queuosine biosynthesis protein queC OS=Synechococc...	[more]
QUEC_NITEU	2.361e-12	32.79	Queuosine biosynthesis protein queC OS=Nitrosomona...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-703-002-F08-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY720144 ID=EY720144; Name=EY720144; organism=Citrus sinensis; type=EST; length=853bp
CTCCAGACCCCCGGGTCCAAGGAAGCGCACCGCAACCGCTACGAGAACAT
CGAGTTGCTGACGGCCAACGACCAGGTCAAGTTCGTCATCTGTTCCCGTG
AAGACTACGACTGGGCGACCTCCAAGCTGATCCAATATGGCCTGGACCGC
CGTGCCGGCGAAGTGCTGTTTTCCCCCAGCCATCATGACCTGAATGCTCG
CGACCTGGCTGACTGGGTTGTCGCAGACAATCTGCCGGTGCGCCTGCAGC
TGCAGTTGCACAAGTACCTGTGGAACGACGAGCCGGGGCGCTGAGATGAC
TGATCAAAAGCGTGCTGTCATCCTGCTGTCCGGCGGCCTCGACTCCGCCA
CCGTGGTCGCCATGGCCCGTGCAGATGGCTACAGCTGCTACACCATGAGC
TTCGACTACGGCCAGCGTCACCGTGCCGAGCTGGAGGCTGCTGCCCGCGT
CGCCCGAGACCTGGGCGTGGTGGAGCACAGGTGATCGGGCTGAACCTCGA
CGGCATGGGTGGTTCGGCGCTGACCGACAGCTCCATAGACGTGCCGCAAG
GCCCCAGCGAAGGGATCCCGGGTGACTAAGTGGCTGCGCGGTACCACGTC
CTGCTGTACCCTGGCCCTGGGTTGGGGCCGAATTCTCGAGGCGCGCCACA
TTTTCGTCGGCGGTCACCGCCCTGTATTACGCCGGGTACCCGGACTGCCG
CCCCTATTATCTCGAGTCCTGCGAGCGCGGGGCCACCTCCCCACCATGGG
CGGTGTTAAATGGGCGGGTTTTGGCTTCTGTGCGCCGCGTGGGAAACGGT
GCGCGGGCGAGGTCCTGACAGGGCGGCGCAGGGCAGGGGCGCTCTGCCCC
GCG