EY692869 | Citrus Genome Database

Overview


Name	EY692869
Unique Name	EY692869
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	946

Libraries


Library Name	Type
Sweet orange fruit, development stadium (1 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y3384_GEOMG (Putative phosphotransferase Gmet_3384 OS=Geobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210) GN=Gmet_3384 PE=3 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 8.247e-24
Identity = 31/111 (27.93%), Postives = 54/111 (48.65%), Query Frame = 2

Query:  281 KSIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLP 613
            + IY+ SD TG T E  + AAL QF      R+     H  S I   E ++  ++   K+  +++YTL D  +A+  +   E  G+ + D++  +   ++   GV+P   P
Sbjct:    6 RHIYLFSDATGETVERVLRAALSQF------RDVEARIHRMSKIRTREDILSALEDVLKEPGIVIYTLVDAELAQLLRDEAEAHGLDALDLISSLLFKLSDFFGVAPQNEP 110

HSP 2 Score: 47.7506 bits (112), Expect = 8.247e-24
Identity = 22/45 (48.89%), Postives = 30/45 (66.67%), Query Frame = 1

Query:  634 FPLSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            + L+ EY +RIEA++FT   DDG   + L KAD +L  VSR+ KT
Sbjct:  114 YELNTEYHKRIEAVDFTVKHDDGQDPRGLSKADFVLVGVSRSSKT 158

HSP 3 Score: 46.2098 bits (108), Expect = 8.247e-24
Identity = 22/37 (59.46%), Postives = 28/37 (75.68%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP-RVFSVDQKR 869
            K  LS+YLA KGYKVANVP+V G++ P  +F VDQ +
Sbjct:  157 KTPLSMYLAHKGYKVANVPLVKGIDPPAELFKVDQNK 193

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y997_GEOLS (Putative phosphotransferase Glov_0997 OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=Glov_0997 PE=3 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.494e-21
Identity = 34/111 (30.63%), Postives = 53/111 (47.75%), Query Frame = 2

Query:  281 KSIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLP 613
            K IY++SD TG TAE  V AAL QF          V     + + + +++   +     D  +LVYTL D S+A +     E  G+ + D+L P+  +++  LG      P
Sbjct:    2 KKIYVLSDSTGETAERVVRAALSQF------GGSDVRIVRLAKVCNQQEVQQAVATVTADEGLLVYTLVDSSLAVAVHTIAEEHGLMAFDLLSPLLHSLSVFLGTVAQSTP 106

HSP 2 Score: 47.3654 bits (111), Expect = 1.494e-21
Identity = 21/43 (48.84%), Postives = 30/43 (69.77%), Query Frame = 1

Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            +  +YFRR+EA+ FT   DDG  ++ L KAD++L  VSR+ KT
Sbjct:  112 IDTDYFRRMEAVNFTVKHDDGQENRGLVKADLVLVGVSRSSKT 154

HSP 3 Score: 41.5874 bits (96), Expect = 1.494e-21
Identity = 18/27 (66.67%), Postives = 22/27 (81.48%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP 842
            K  LS+YLA KGYKVANVP+V G++ P
Sbjct:  153 KTPLSMYLANKGYKVANVPLVKGIDPP 179

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y242_GEOBB (Putative phosphotransferase Gbem_0242 OS=Geobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622) GN=Gbem_0242 PE=3 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 4.220e-21
Identity = 23/46 (50.00%), Postives = 34/46 (73.91%), Query Frame = 1

Query:  631 KFPLSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            ++ ++ EY+RR+EA++FT   DDG   +NL KADI+L  VSR+ KT
Sbjct:  109 QYEMNSEYYRRMEAVDFTVKQDDGQEPRNLHKADIVLIGVSRSSKT 154

HSP 2 Score: 46.9802 bits (110), Expect = 4.220e-21
Identity = 27/109 (24.77%), Postives = 51/109 (46.79%), Query Frame = 2

Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLP 613
            +Y++SD TG T E    AAL QF      R+  +       I + E ++  + +  +   ++ YTL +  +A+  +   E   + + D++ P+   +A  L + P  LP
Sbjct:    4 VYLLSDATGETVERVARAALTQF------RDVDIRLRRMGQIRNREDILRALDEVDRAPGIIFYTLVNTELAQFVRNEVEARQLEAVDLITPLLYKLAEFLEMRPQKLP 106

HSP 3 Score: 42.3578 bits (98), Expect = 4.220e-21
Identity = 18/27 (66.67%), Postives = 22/27 (81.48%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP 842
            K  LS+YLA KGYKVANVPI+ G++ P
Sbjct:  153 KTPLSMYLAHKGYKVANVPIIQGIDPP 179

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y226_GEOSM (Putative phosphotransferase GM21_0226 OS=Geobacter sp. (strain M21) GN=GM21_0226 PE=3 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 4.220e-21
Identity = 23/46 (50.00%), Postives = 34/46 (73.91%), Query Frame = 1

Query:  631 KFPLSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            ++ ++ EY+RR+EA++FT   DDG   +NL KADI+L  VSR+ KT
Sbjct:  109 QYEMNSEYYRRMEAVDFTVKQDDGQEPRNLHKADIVLIGVSRSSKT 154

HSP 2 Score: 46.9802 bits (110), Expect = 4.220e-21
Identity = 27/109 (24.77%), Postives = 51/109 (46.79%), Query Frame = 2

Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLP 613
            +Y++SD TG T E    AAL QF      R+  +       I + E ++  + +  +   ++ YTL +  +A+  +   E   + + D++ P+   +A  L + P  LP
Sbjct:    4 VYLLSDATGETVERVARAALTQF------RDVDIRLRRMGQIRNREDILRALDEVDRAPGIIFYTLVNTELAQFVRNEVEARQLEAVDLITPLLYKLAEFLEMRPQKLP 106

HSP 3 Score: 42.3578 bits (98), Expect = 4.220e-21
Identity = 18/27 (66.67%), Postives = 22/27 (81.48%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP 842
            K  LS+YLA KGYKVANVPI+ G++ P
Sbjct:  153 KTPLSMYLAHKGYKVANVPIIQGIDPP 179

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y117_NOVAD (Putative phosphotransferase Saro_0117 OS=Novosphingobium aromaticivorans (strain DSM 12444) GN=Saro_0117 PE=3 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 5.463e-21
Identity = 36/129 (27.91%), Postives = 64/129 (49.61%), Query Frame = 2

Query:  272 MEGKSIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLPRGAPGRNFLLVRSTF 658
            M+   ++++SD TG T E    AAL QF+   V R      H +  +  ++ L  I+ + A +  +++YTL +    E  ++ C   G+PS   L  +T+A+   LG+      +G PGR  ++  + F
Sbjct:    1 MQRLHLHLLSDSTGETLEMIAKAALAQFDGADVVR------HFWPMVRSMQHLDRIMGEIAANPGLVLYTLVNAETRERLEQRCAALGLPSVAALDAVTDALQQQLGIQ----AKGRPGRQHMMDEAYF 119

HSP 2 Score: 47.7506 bits (112), Expect = 5.463e-21
Identity = 21/43 (48.84%), Postives = 31/43 (72.09%), Query Frame = 1

Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            + E YF R++AI+FT   DDG   +N ++ADI+L+ VSR+ KT
Sbjct:  114 MDEAYFARVDAIQFTIAHDDGVGWENWEEADIVLAGVSRSSKT 156

HSP 3 Score: 35.4242 bits (80), Expect = 5.463e-21
Identity = 18/27 (66.67%), Postives = 20/27 (74.07%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP 842
            K   SIYLA +GYKVAN+PIV  VE P
Sbjct:  155 KTPTSIYLANRGYKVANIPIV--VESP 179

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y2354_BACA2 (Putative phosphotransferase RBAM_023540 OS=Bacillus amyloliquefaciens (strain FZB42) GN=RBAM_023540 PE=3 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 7.093e-21
Identity = 32/111 (28.83%), Postives = 57/111 (51.35%), Query Frame = 2

Query:  272 MEGKSIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHL--FSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVS 598
            M+ + IY+VSD  G TAE  V A+L QF         A +TH+     ++D+  +  ++  A  DG ++ +TL  P M +   +  E   +   D++GP+ + + S  G++
Sbjct:    1 MDNRIIYVVSDSVGETAELVVKASLSQFN------GSADDTHIRRIPYVEDIGTINEVVSLAKADGGIICFTLVVPEMRKYLVEQAEKANVLYYDIIGPLIDKMESAYGLT 105

HSP 2 Score: 50.447 bits (119), Expect = 7.093e-21
Identity = 24/43 (55.81%), Postives = 30/43 (69.77%), Query Frame = 1

Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L E+YF+++EAIEF    DDG   + + KADIIL  VSRT KT
Sbjct:  116 LDEDYFKKVEAIEFAVKYDDGRDPRGILKADIILLGVSRTSKT 158

HSP 3 Score: 37.3502 bits (85), Expect = 7.093e-21
Identity = 22/37 (59.46%), Postives = 25/37 (67.57%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP-RVFSVDQKR 869
            K  LS YLA K  KVANVPIV  V+ P  +FSVD K+
Sbjct:  157 KTPLSQYLAHKRLKVANVPIVPEVDPPEELFSVDPKK 193

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y585_SYMTH (Putative phosphotransferase STH585 OS=Symbiobacterium thermophilum GN=STH585 PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 3.363e-20
Identity = 35/111 (31.53%), Postives = 59/111 (53.15%), Query Frame = 2

Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLPRG 619
            I++VSD  G TA+    AAL Q+         +V    F  IDD + L  +I  A +   ++VYT+  P + +  ++  ++W IP+ D++GP+ + I+  LG +P  L  G
Sbjct:    7 IFVVSDSVGETADLVTRAALSQWGQR------SVEIRRFPMIDDPQSLREVIDAARQQPTLIVYTIIVPEIRKELQRLVDMWDIPAVDIMGPMLDGISRLLG-APEKLEPG 110

HSP 2 Score: 45.8246 bits (107), Expect = 3.363e-20
Identity = 21/43 (48.84%), Postives = 28/43 (65.12%), Query Frame = 1

Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L ++YFRRIEA+EF    DDG   +   +AD +L  VSR+ KT
Sbjct:  115 LDDDYFRRIEAVEFAVKYDDGKDPRGFLRADAVLIGVSRSSKT 157

HSP 3 Score: 32.7278 bits (73), Expect = 3.363e-20
Identity = 14/28 (50.00%), Postives = 20/28 (71.43%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECPR 845
            K  +S+YLA + YKVAN+P+V  V  P+
Sbjct:  156 KTPVSMYLAHRRYKVANLPLVPEVSLPK 183

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: YQFL_BACSU (Putative phosphotransferase yqfL OS=Bacillus subtilis GN=yqfL PE=1 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 7.326e-20
Identity = 33/111 (29.73%), Postives = 55/111 (49.55%), Query Frame = 2

Query:  272 MEGKSIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHL--FSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVS 598
            M  + IY+VSD  G TAE  V AAL QF         A +TH+     ++D+  +  +I  A  DG ++ +TL  P + E      E   +   D++GP+ + + +  G++
Sbjct:    1 MNNRIIYVVSDSVGETAELVVKAALSQF------NGSADDTHVRRIPYVEDIGTINEVISLAKADGGIICFTLVVPEIREYLIAEAEKANVLYYDIIGPLIDKMETAYGLT 105

HSP 2 Score: 50.0618 bits (118), Expect = 7.326e-20
Identity = 23/43 (53.49%), Postives = 30/43 (69.77%), Query Frame = 1

Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L E+YF+++EAIEF    DDG   + + KADI+L  VSRT KT
Sbjct:  116 LDEDYFKKVEAIEFAVKYDDGRDPRGILKADIVLIGVSRTSKT 158

HSP 3 Score: 36.1946 bits (82), Expect = 7.326e-20
Identity = 21/37 (56.76%), Postives = 25/37 (67.57%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP-RVFSVDQKR 869
            K  LS YLA K  KVANVPIV  V+ P  +F+VD K+
Sbjct:  157 KTPLSQYLAHKRLKVANVPIVPEVDPPEELFNVDPKK 193

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y1513_SYNWW (Putative phosphotransferase Swol_1513 OS=Syntrophomonas wolfei subsp. wolfei (strain Goettingen) GN=Swol_1513 PE=3 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 2.065e-19
Identity = 38/109 (34.86%), Postives = 50/109 (45.87%), Query Frame = 2

Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLP 613
            +++VSD  G TAE  V AA  QF    ++     N      I D E L  II QAA    ++ YTL    +A+  K      G+   DVLGP+ EA  S   + P   P
Sbjct:    8 VFIVSDSIGETAEMVVRAAASQFNSGNMEIRQVPN------ISDTETLEEIINQAAASNFIIAYTLVINELADFLKMEARKKGVICVDVLGPVIEAFKSVSDIEPRREP 110

HSP 2 Score: 45.8246 bits (107), Expect = 2.065e-19
Identity = 20/43 (46.51%), Postives = 29/43 (67.44%), Query Frame = 1

Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            + E Y+RR+EA+EF    DDG   + + ++DI+L  VSRT KT
Sbjct:  116 VDEMYYRRVEAVEFAVRYDDGKDPRGVMQSDIVLVGVSRTSKT 158

HSP 3 Score: 35.039 bits (79), Expect = 2.065e-19
Identity = 19/35 (54.29%), Postives = 24/35 (68.57%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP-RVFSVDQ 863
            K  LS+YLA K  KVANVP+V  VE P  +F V++
Sbjct:  157 KTPLSMYLAHKRIKVANVPLVPEVEPPEEIFQVEK 191

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: YD000_ERYLH (Putative phosphotransferase ELI_13000 OS=Erythrobacter litoralis (strain HTCC2594) GN=ELI_13000 PE=3 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 2.675e-19
Identity = 29/109 (26.61%), Postives = 51/109 (46.79%), Query Frame = 2

Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLP 613
            ++++SD TG T E    AAL QFE      N  V  H +  +   + L  I+ + + +  ++++TL +     + +  C    +P+ D L  +T A+   LG    G P
Sbjct:    6 LHLLSDSTGETLEMIAKAALAQFE------NADVVRHFWPMVRSRQHLERIVPELSNNPGLVLFTLVNAETRRALRDHCRRLSLPAVDALDEVTAALEVQLGQEAHGRP 108

HSP 2 Score: 50.0618 bits (118), Expect = 2.675e-19
Identity = 22/43 (51.16%), Postives = 32/43 (74.42%), Query Frame = 1

Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            + E YF+R+EAI+FT   DDG   +N ++ADI+L+ VSR+ KT
Sbjct:  114 MDEAYFKRVEAIQFTIAHDDGVGWENWEEADIVLAGVSRSSKT 156

HSP 3 Score: 35.8094 bits (81), Expect = 2.675e-19
Identity = 15/22 (68.18%), Postives = 18/22 (81.82%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVM 827
            K   SIYLA +GYKVAN+P+VM
Sbjct:  155 KTPTSIYLANRGYKVANIPLVM 176

The following BLAST results are available for this feature:

BLAST of EY692869 vs. ExPASy Swiss-Prot

BLAST of EY692869 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 146


Match Name	E-value	Identity	Description
Y3384_GEOMG	8.247e-24	27.93	Putative phosphotransferase Gmet_3384 OS=Geobacter...	[more]
Y997_GEOLS	1.494e-21	30.63	Putative phosphotransferase Glov_0997 OS=Geobacter...	[more]
Y242_GEOBB	4.220e-21	50.00	Putative phosphotransferase Gbem_0242 OS=Geobacter...	[more]
Y226_GEOSM	4.220e-21	50.00	Putative phosphotransferase GM21_0226 OS=Geobacter...	[more]
Y117_NOVAD	5.463e-21	27.91	Putative phosphotransferase Saro_0117 OS=Novosphin...	[more]
Y2354_BACA2	7.093e-21	28.83	Putative phosphotransferase RBAM_023540 OS=Bacillu...	[more]
Y585_SYMTH	3.363e-20	31.53	Putative phosphotransferase STH585 OS=Symbiobacter...	[more]
YQFL_BACSU	7.326e-20	29.73	Putative phosphotransferase yqfL OS=Bacillus subti...	[more]
Y1513_SYNWW	2.065e-19	34.86	Putative phosphotransferase Swol_1513 OS=Syntropho...	[more]
YD000_ERYLH	2.675e-19	26.61	Putative phosphotransferase ELI_13000 OS=Erythroba...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-700-005-C11-CT.F Sweet orange fruit, development stadium (1 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY692869 ID=EY692869; Name=EY692869; organism=Citrus sinensis; type=EST; length=946bp
CCACGCGTCCGACCTCCCGAACCTCCACTCCACGCCGACCGCCCCCGGCC
AAACCACATCCGAGCCTGACTCTAAACCCCAGGCTCGGAAACTGAAGAGC
AGCCCTCAGCTGAACCGCTGGTCGAGGGCCCGCGCTGTACGATCAGGTCG
TAAATTGGACCGGTTGGGCCACCGGTCCCCCCAGTCGTCTGTTAATTTTA
AAGTTAGAGACAGAGATGGTGTTGATTTGGAGCCGGAAAGAATAAGTGAC
GGCGAGGATAACGTGGAGGCGATGGAAGGGAAGTCCATTTACATGGTCTC
TGACGGCACTGGATGGACGGCGGAGCACGCCGTTAATGCTGCGTTGGGGC
AGTTCGAGCACTGTTTAGTAGATCGAAACTGTGCTGTAAATACCCACCTC
TTCTCAGGGATTGATGATGTAGAGCAGTTGATGGTGATAATAAAGCAAGC
AGCCAAGGACGGGGCAATGCTTGTTTACACCTTAGCCGATCCTTCCATGG
CGGAGTCTGCTAAAAAGGCCTGCGAGCTTTGGGGTATACCGTCCACTGAC
GTATTGGGACCGATTACGGAGGCAATTGCATCCCATCTAGGTGTCTCACC
TTCTGGCCTTCCCCGCGGTGCTCCCGGCCGAAATTTCCTCTTAGTGAGGA
GTACTTTCGTCGCATAGAGGCTATTGAATTTACCATNNTAGCAGATGATG
GTGCCCCTTCACAAAATTTGCAAAAAGCCGACATTATTCTATCANGTGTT
TCTCGTACTGGAAAAACACGTTATCCATCTATTTAGCTCAGAAGGGATAC
AAAGTGGCAAATGTCCCAATTGTGATGGGTGTAGAATGCCCTAGAGTCTT
TTCAGTAGACCAGAAAAGGTTTTGGGNTTGACATNATCTCTAGTCTGCAT
CTATAAAAAGCAGAGCTAGAGTTGGGATTCGAGATGAATAGGAGCA