EY692869

Overview
NameEY692869
Unique NameEY692869
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length946
Libraries
Library NameType
Sweet orange fruit, development stadium (1 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y1250_STAES (Putative phosphotransferase SE_1250 OS=Staphylococcus epidermidis (strain ATCC 12228) GN=SE_1250 PE=3 SV=1)

HSP 1 Score: 47.7506 bits (112), Expect = 1.726e-16
Identity = 22/43 (51.16%), Postives = 29/43 (67.44%), Query Frame = 1 
Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L + YF++I+AIEF    DDG   + L KADI+L  +SRT KT
Sbjct:  116 LDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKT 158

HSP 2 Score: 45.4394 bits (106), Expect = 1.726e-16
Identity = 28/110 (25.45%), Postives = 50/110 (45.45%), Query Frame = 2 
Query:  272 MEGKSIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSP 601
            M+   I + SD  G TAE    A + QF      + C      +  I+  E +  +I+ A    A++VYTL  P + +          + S D++GP+ E +++ +  +P
Sbjct:    1 MDNIKIIVASDSIGETAELVARAGVSQFN----PKQCKHEFLRYPYIESFENVDEVIQVAKDTNAIIVYTLIKPEIKKYMISKVNEHALKSVDIMGPLMELLSNSIEETP 106

HSP 3 Score: 33.8834 bits (76), Expect = 1.726e-16
Identity = 19/37 (51.35%), Postives = 23/37 (62.16%), Query Frame = 3 
Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP-RVFSVDQKR 869
            K  LS YLA K YKV N+PIV  V  P  +F+VD  +
Sbjct:  157 KTPLSQYLAHKSYKVMNIPIVPEVTPPDGLFNVDPSK 193
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y1129_STAEQ (Putative phosphotransferase SERP1129 OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=SERP1129 PE=3 SV=1)

HSP 1 Score: 47.7506 bits (112), Expect = 1.726e-16
Identity = 22/43 (51.16%), Postives = 29/43 (67.44%), Query Frame = 1 
Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L + YF++I+AIEF    DDG   + L KADI+L  +SRT KT
Sbjct:  116 LDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKT 158

HSP 2 Score: 45.4394 bits (106), Expect = 1.726e-16
Identity = 28/110 (25.45%), Postives = 50/110 (45.45%), Query Frame = 2 
Query:  272 MEGKSIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSP 601
            M+   I + SD  G TAE    A + QF      + C      +  I+  E +  +I+ A    A++VYTL  P + +          + S D++GP+ E +++ +  +P
Sbjct:    1 MDNIKIIVASDSIGETAELVARAGVSQFN----PKQCKHEFLRYPYIESFENVDEVIQVAKDTNAIIVYTLIKPEIKKYMISKVNEHALKSVDIMGPLMELLSNSIEETP 106

HSP 3 Score: 33.8834 bits (76), Expect = 1.726e-16
Identity = 19/37 (51.35%), Postives = 23/37 (62.16%), Query Frame = 3 
Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP-RVFSVDQKR 869
            K  LS YLA K YKV N+PIV  V  P  +F+VD  +
Sbjct:  157 KTPLSQYLAHKSYKVMNIPIVPEVTPPDGLFNVDPSK 193
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y4385_OLICO (Putative phosphotransferase OCAR_4385 OS=Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / OM5) GN=OCAR_4385 PE=3 SV=1)

HSP 1 Score: 47.3654 bits (111), Expect = 2.230e-16
Identity = 24/53 (45.28%), Postives = 34/53 (64.15%), Query Frame = 1 
Query:  610 SPRCSRPKFPLSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            SPR    +  L+ EYF RI+A+ +T   DDG   + L++AD++L  VSRT KT
Sbjct:  110 SPRVGA-QHTLNAEYFNRIDALNYTMMHDDGQHVEGLEEADVVLVGVSRTSKT 161

HSP 2 Score: 46.2098 bits (108), Expect = 2.230e-16
Identity = 24/102 (23.53%), Postives = 49/102 (48.04%), Query Frame = 2 
Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLG 592
            ++M+SD TG T      A   Q+       N     H++  +   +QL  ++ +  +   ++++TL +  + E  +  C+   IPS  ++GP+ E   ++LG
Sbjct:   11 LHMISDSTGETLIMVARAVAAQYA------NVTPVEHVYPLVRSQKQLDRVLAEIEEAPGIVLFTLLEKDLVERVEATCKDMNIPSLSIIGPVMELFRAYLG 106

HSP 3 Score: 33.113 bits (74), Expect = 2.230e-16
Identity = 14/27 (51.85%), Postives = 18/27 (66.67%), Query Frame = 3 
Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP 842
            K   SIYLA +G + ANVP+V G+  P
Sbjct:  160 KTPTSIYLANRGIRTANVPLVPGIPIP 186
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y1193_STAS1 (Putative phosphotransferase SSP1193 OS=Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) GN=SSP1193 PE=3 SV=1)

HSP 1 Score: 48.1358 bits (113), Expect = 2.892e-16
Identity = 23/43 (53.49%), Postives = 29/43 (67.44%), Query Frame = 1 
Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L E YF++I+AIEF    DDG   + L KADI+L  +SRT KT
Sbjct:  116 LDEAYFKKIDAIEFAVKYDDGKDPKGLFKADIVLLGISRTSKT 158

HSP 2 Score: 44.2838 bits (103), Expect = 2.892e-16
Identity = 27/110 (24.55%), Postives = 48/110 (43.64%), Query Frame = 2 
Query:  272 MEGKSIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSP 601
            M+   I + SD  G TAE    A + QF      + C      +  I+ +E +  +I  A    A++VYTL  P + +  +     + I + D++GP+   +   +   P
Sbjct:    1 MKNIQIIVASDSVGETAELVARACISQFN----PKQCESEISRYPYIEALENVDEVISVAKDTEAIVVYTLVKPEIRKYMEAKLAEFKIKAVDIMGPLMNILLDQIDEKP 106

HSP 3 Score: 33.8834 bits (76), Expect = 2.892e-16
Identity = 18/37 (48.65%), Postives = 23/37 (62.16%), Query Frame = 3 
Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP-RVFSVDQKR 869
            K  LS YLA K YKV N+PIV  V  P  +F++D  +
Sbjct:  157 KTPLSQYLAHKSYKVMNIPIVPEVTPPDDLFNIDSSK 193
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y199_BRASO (Putative phosphotransferase BRADO0199 OS=Bradyrhizobium sp. (strain ORS278) GN=BRADO0199 PE=3 SV=1)

HSP 1 Score: 45.8246 bits (107), Expect = 4.841e-16
Identity = 24/107 (22.43%), Postives = 51/107 (47.66%), Query Frame = 2 
Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSG 607
            +++VSD TG T      A   Q+       N     H++  +   +QL  ++ +  +   ++++TL +  +    +  C+   IPS  ++GP+ +   ++LG S +G
Sbjct:   10 LHLVSDSTGETLITVARAVAAQYA------NVTPVEHVYPLVRSQKQLDRVLDEIEEAPGIVLFTLLEKDLVSRLEAKCQQINIPSLSIIGPVMQLFEAYLGASTAG 110

HSP 2 Score: 45.0542 bits (105), Expect = 4.841e-16
Identity = 21/43 (48.84%), Postives = 28/43 (65.12%), Query Frame = 1 
Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L+ EYF RI+A+ +T   DDG     L+ AD++L  VSRT KT
Sbjct:  118 LNAEYFARIDALNYTMLHDDGQMVDGLEDADVVLVGVSRTSKT 160

HSP 3 Score: 34.6538 bits (78), Expect = 4.841e-16
Identity = 16/35 (45.71%), Postives = 21/35 (60.00%), Query Frame = 3 
Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECPRVFSVDQK 866
            K   SIYLA +G + ANVP+V G+  P    V +K
Sbjct:  159 KTPTSIYLANRGVRTANVPLVPGIPIPHQLEVLKK 193
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y001_AGRVS (Putative phosphotransferase Avi_0001 OS=Agrobacterium vitis (strain S4 / ATCC BAA-846) GN=Avi_0001 PE=3 SV=1)

HSP 1 Score: 50.8322 bits (120), Expect = 6.274e-16
Identity = 28/102 (27.45%), Postives = 52/102 (50.98%), Query Frame = 2 
Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLG 592
            ++++SD TG T   A  AA  QF H           H++  + +  Q   +++   K+  +++YT+ DP +A+   + C   G+PS +VL P+     ++LG
Sbjct:   10 LHLISDSTGETLISAGRAAAVQFPHSNPIE------HVYPLVRNRRQADAVLQSIDKEPGIVLYTIVDPELADFIDRRCIEIGVPSVNVLEPVIGTFQAYLG 105

HSP 2 Score: 44.2838 bits (103), Expect = 6.274e-16
Identity = 18/43 (41.86%), Postives = 30/43 (69.77%), Query Frame = 1 
Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            ++ +YF RIEA+ +T   DDG  +++  +AD++L  +SRT KT
Sbjct:  118 MNADYFARIEALNYTMDHDDGQMAEDYDQADVVLVGISRTSKT 160

HSP 3 Score: 30.0314 bits (66), Expect = 6.274e-16
Identity = 13/21 (61.90%), Postives = 15/21 (71.43%), Query Frame = 3 
Query:  762 KNTLSIYLAQKGYKVANVPIV 824
            K   SIYLA +G K AN+PIV
Sbjct:  159 KTPTSIYLANRGIKTANIPIV 179
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y002_RHIE6 (Putative phosphotransferase RHECIAT_CH0000002 OS=Rhizobium etli (strain CIAT 652) GN=RHECIAT_CH0000002 PE=3 SV=1)

HSP 1 Score: 46.9802 bits (110), Expect = 6.274e-16
Identity = 32/112 (28.57%), Postives = 56/112 (50.00%), Query Frame = 2 
Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLPRGA 622
            ++++SD TG T   A  AA  QF      R+     H++  I + +QL+ +++       +++YT+ D  +A    + C   G+ S +VL P+  A   +LG +PS    GA
Sbjct:   10 LHLISDSTGETLISAGRAASAQF------RSAQPIEHVYPLIRNRKQLLPVLQAIDDAPGIVLYTIVDRELASLIDERCTEMGVASVNVLEPVMNAFQIYLG-APSRRRVGA 114

HSP 2 Score: 42.3578 bits (98), Expect = 6.274e-16
Identity = 21/54 (38.89%), Postives = 30/54 (55.56%), Query Frame = 1 
Query:  607 PSPRCSRPKFPLSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            PS R    +  ++  YF RIEA+ FT   DDG    +   AD+++  +SRT KT
Sbjct:  107 PSRRRVGAQHVMNAGYFARIEALNFTMDHDDGQMPDDYNDADVVIIGISRTSKT 160

HSP 3 Score: 35.8094 bits (81), Expect = 6.274e-16
Identity = 18/35 (51.43%), Postives = 20/35 (57.14%), Query Frame = 3 
Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECPRVFSVDQK 866
            K   SIYLA +G K AN+PIV GV  P    V  K
Sbjct:  159 KTPTSIYLANRGIKTANIPIVYGVPLPEGLLVATK 193
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y426_RHOPS (Putative phosphotransferase RPD_0426 OS=Rhodopseudomonas palustris (strain BisB5) GN=RPD_0426 PE=3 SV=1)

HSP 1 Score: 46.2098 bits (108), Expect = 1.050e-15
Identity = 20/43 (46.51%), Postives = 31/43 (72.09%), Query Frame = 1 
Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L+ EYF+RI+A+ ++   DDG   + L++AD++L  VSRT KT
Sbjct:  118 LNAEYFKRIDALNYSMMHDDGQHVEGLEEADVVLVGVSRTSKT 160

HSP 2 Score: 44.669 bits (104), Expect = 1.050e-15
Identity = 23/107 (21.50%), Postives = 52/107 (48.60%), Query Frame = 2 
Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSG 607
            +++VSD TG T      A   Q+       N +   H++  +   +QL  ++++  +   ++++TL +  +    +  C+    PS  ++GP+ +   ++LG S +G
Sbjct:   10 LHLVSDSTGETLITVSRAVAAQYA------NVSPVEHVYPLVRSQKQLDRVLQEIEESPGIVLFTLLESELVNRLEAKCQQINSPSLSIIGPVMQLFEAYLGASTTG 110

HSP 3 Score: 33.4982 bits (75), Expect = 1.050e-15
Identity = 14/27 (51.85%), Postives = 18/27 (66.67%), Query Frame = 3 
Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP 842
            K   SIYLA +G + ANVP+V G+  P
Sbjct:  159 KTPTSIYLANRGIRTANVPLVAGIPIP 185
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y001_AGRT5 (Putative phosphotransferase Atu0001 OS=Agrobacterium tumefaciens (strain C58 / ATCC 33970) GN=Atu0001 PE=3 SV=1)

HSP 1 Score: 48.521 bits (114), Expect = 1.051e-15
Identity = 33/112 (29.46%), Postives = 54/112 (48.21%), Query Frame = 2 
Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLPRGA 622
            ++++SD TG T   A  A   QF   +         H++  I + +QL  +I    K+  +++YT+ D  +AE     C   G+P  +VL PI     ++LG +PS    GA
Sbjct:   10 LHLISDSTGETLMSAGRAVSAQFHTSMPVE------HVYPMIRNQKQLAQVIDLIDKEPGIVLYTIVDQQLAEFLDLRCHAIGVPCVNVLEPIIGIFQTYLG-APSRRRVGA 114

HSP 2 Score: 43.5134 bits (101), Expect = 1.051e-15
Identity = 21/54 (38.89%), Postives = 30/54 (55.56%), Query Frame = 1 
Query:  607 PSPRCSRPKFPLSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            PS R    +  L+ +YF RIEA+ F    DDG   +    AD+++  +SRT KT
Sbjct:  107 PSRRRVGAQHALNADYFARIEALNFAMDHDDGQMPETYDDADVVIIGISRTSKT 160

HSP 3 Score: 32.3426 bits (72), Expect = 1.051e-15
Identity = 14/27 (51.85%), Postives = 17/27 (62.96%), Query Frame = 3 
Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP 842
            K   SIYLA +G K AN+P+V  V  P
Sbjct:  159 KTPTSIYLANRGIKTANIPVVPNVPLP 185
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y001_RHIEC (Putative phosphotransferase RHE_CH00001 OS=Rhizobium etli (strain CFN 42 / ATCC 51251) GN=RHE_CH00001 PE=3 SV=1)

HSP 1 Score: 46.595 bits (109), Expect = 1.051e-15
Identity = 32/112 (28.57%), Postives = 56/112 (50.00%), Query Frame = 2 
Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLPRGA 622
            ++++SD TG T   A  AA  QF      R+     H++  I + +QL+ +++       +++YT+ D  +A    + C   G+ S +VL P+  A   +LG +PS    GA
Sbjct:   10 LHLISDSTGETLISAGRAASAQF------RSAQPIEHVYPLIRNRKQLLPVLQAIDDAPGIVLYTIVDRELANLIDERCVEMGVASVNVLEPVMNAFQIYLG-APSRRRVGA 114

HSP 2 Score: 42.3578 bits (98), Expect = 1.051e-15
Identity = 21/54 (38.89%), Postives = 30/54 (55.56%), Query Frame = 1 
Query:  607 PSPRCSRPKFPLSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            PS R    +  ++  YF RIEA+ FT   DDG    +   AD+++  +SRT KT
Sbjct:  107 PSRRRVGAQHVMNAGYFARIEALNFTMDHDDGQMPDDYNDADVVIIGISRTSKT 160

HSP 3 Score: 35.4242 bits (80), Expect = 1.051e-15
Identity = 16/27 (59.26%), Postives = 18/27 (66.67%), Query Frame = 3 
Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP 842
            K   SIYLA +G K AN+PIV GV  P
Sbjct:  159 KTPTSIYLANRGIKTANIPIVYGVPLP 185
The following BLAST results are available for this feature:
BLAST of EY692869 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 146
Match NameE-valueIdentityDescription
Y1250_STAES1.726e-1651.16Putative phosphotransferase SE_1250 OS=Staphylococ... [more]
Y1129_STAEQ1.726e-1651.16Putative phosphotransferase SERP1129 OS=Staphyloco... [more]
Y4385_OLICO2.230e-1645.28Putative phosphotransferase OCAR_4385 OS=Oligotrop... [more]
Y1193_STAS12.892e-1653.49Putative phosphotransferase SSP1193 OS=Staphylococ... [more]
Y199_BRASO4.841e-1622.43Putative phosphotransferase BRADO0199 OS=Bradyrhiz... [more]
Y001_AGRVS6.274e-1627.45Putative phosphotransferase Avi_0001 OS=Agrobacter... [more]
Y002_RHIE66.274e-1628.57Putative phosphotransferase RHECIAT_CH0000002 OS=R... [more]
Y426_RHOPS1.050e-1546.51Putative phosphotransferase RPD_0426 OS=Rhodopseud... [more]
Y001_AGRT51.051e-1529.46Putative phosphotransferase Atu0001 OS=Agrobacteri... [more]
Y001_RHIEC1.051e-1528.57Putative phosphotransferase RHE_CH00001 OS=Rhizobi... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-700-005-C11-CT.F Sweet orange fruit, development stadium (1 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY692869 ID=EY692869; Name=EY692869; organism=Citrus sinensis; type=EST; length=946bp
CCACGCGTCCGACCTCCCGAACCTCCACTCCACGCCGACCGCCCCCGGCC
AAACCACATCCGAGCCTGACTCTAAACCCCAGGCTCGGAAACTGAAGAGC
AGCCCTCAGCTGAACCGCTGGTCGAGGGCCCGCGCTGTACGATCAGGTCG
TAAATTGGACCGGTTGGGCCACCGGTCCCCCCAGTCGTCTGTTAATTTTA
AAGTTAGAGACAGAGATGGTGTTGATTTGGAGCCGGAAAGAATAAGTGAC
GGCGAGGATAACGTGGAGGCGATGGAAGGGAAGTCCATTTACATGGTCTC
TGACGGCACTGGATGGACGGCGGAGCACGCCGTTAATGCTGCGTTGGGGC
AGTTCGAGCACTGTTTAGTAGATCGAAACTGTGCTGTAAATACCCACCTC
TTCTCAGGGATTGATGATGTAGAGCAGTTGATGGTGATAATAAAGCAAGC
AGCCAAGGACGGGGCAATGCTTGTTTACACCTTAGCCGATCCTTCCATGG
CGGAGTCTGCTAAAAAGGCCTGCGAGCTTTGGGGTATACCGTCCACTGAC
GTATTGGGACCGATTACGGAGGCAATTGCATCCCATCTAGGTGTCTCACC
TTCTGGCCTTCCCCGCGGTGCTCCCGGCCGAAATTTCCTCTTAGTGAGGA
GTACTTTCGTCGCATAGAGGCTATTGAATTTACCATNNTAGCAGATGATG
GTGCCCCTTCACAAAATTTGCAAAAAGCCGACATTATTCTATCANGTGTT
TCTCGTACTGGAAAAACACGTTATCCATCTATTTAGCTCAGAAGGGATAC
AAAGTGGCAAATGTCCCAATTGTGATGGGTGTAGAATGCCCTAGAGTCTT
TTCAGTAGACCAGAAAAGGTTTTGGGNTTGACATNATCTCTAGTCTGCAT
CTATAAAAAGCAGAGCTAGAGTTGGGATTCGAGATGAATAGGAGCA
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