EY692869

Overview
NameEY692869
Unique NameEY692869
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length946
Libraries
Library NameType
Sweet orange fruit, development stadium (1 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y3095_CLOBM (Putative phosphotransferase CLK_3095 OS=Clostridium botulinum (strain Loch Maree / Type A3) GN=CLK_3095 PE=3 SV=1)

HSP 1 Score: 45.0542 bits (105), Expect = 8.284e-15
Identity = 37/110 (33.64%), Postives = 53/110 (48.18%), Query Frame = 2 
Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCL-VDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLP 613
            IY VSD  G TAE   NA   QF   + V+R   V T      +DV  L+ IIK    + AM++ T+    + E   + C   GI  ++VLGP    +++ L  +P   P
Sbjct:    4 IYAVSDSIGETAEQVANATAYQFGSSVKVERVPYVKT-----FEDVNNLISIIKN--PNEAMIISTIVLVDIREFLVQRCVESGIHISNVLGPCISLVSTILNKTPEYKP 106

HSP 2 Score: 40.4318 bits (93), Expect = 8.284e-15
Identity = 17/43 (39.53%), Postives = 28/43 (65.12%), Query Frame = 1 
Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            + ++Y+++IEA+EF    DD      ++ ADI+L  +SRT KT
Sbjct:  112 MDKKYYKKIEAMEFAIRYDDSKDHSGIKHADIVLIGLSRTSKT 154

HSP 3 Score: 35.8094 bits (81), Expect = 8.284e-15
Identity = 17/37 (45.95%), Postives = 24/37 (64.86%), Query Frame = 3 
Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP-RVFSVDQKR 869
            K  LSIYLA KG K  N+P++  V  P  +F +D+K+
Sbjct:  153 KTPLSIYLANKGIKALNIPLMPEVPVPEELFEIDRKK 189
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y102_NITWN (Putative phosphotransferase Nwi_0102 OS=Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391) GN=Nwi_0102 PE=3 SV=1)

HSP 1 Score: 46.9802 bits (110), Expect = 1.383e-14
Identity = 20/43 (46.51%), Postives = 32/43 (74.42%), Query Frame = 1 
Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L+ +YF+RI+A+ +T   DDG   ++L++AD++L  VSRT KT
Sbjct:  119 LNADYFKRIDALNYTMMHDDGQHVESLEEADVVLVGVSRTSKT 161

HSP 2 Score: 39.2762 bits (90), Expect = 1.383e-14
Identity = 22/107 (20.56%), Postives = 49/107 (45.79%), Query Frame = 2 
Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSG 607
            +++VSD TG T      A   Q+             H++  +   +QL  ++ +  +   ++++TL +  +A   +  C+    PS  ++GP+ +   ++LG   +G
Sbjct:   11 LHLVSDSTGETLITVSRAVTAQYA------GVTPIEHVYPLVRSEKQLDRVLAEIEEAPGIVLFTLLEKDVAVRLEARCQEINCPSLSIIGPVMQLFQAYLGADTTG 111

HSP 3 Score: 34.2686 bits (77), Expect = 1.383e-14
Identity = 14/28 (50.00%), Postives = 19/28 (67.86%), Query Frame = 3 
Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECPR 845
            K   S+YLA +G + ANVP+V G+  PR
Sbjct:  160 KTPTSVYLANRGTRTANVPLVPGIAIPR 187
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y3950_CLOB6 (Putative phosphotransferase CLJ_B3950 OS=Clostridium botulinum (strain 657 / Type Ba4) GN=CLJ_B3950 PE=3 SV=1)

HSP 1 Score: 43.5134 bits (101), Expect = 2.321e-14
Identity = 36/110 (32.73%), Postives = 53/110 (48.18%), Query Frame = 2 
Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCL-VDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLP 613
            IY VSD  G TAE   NA   QF   + V+R   V T      +DV  L+ II+    + AM++ T+    + E   + C   GI  ++VLGP    +++ L  +P   P
Sbjct:    4 IYAVSDSIGETAEQVANATAYQFGSSVKVERVPYVKT-----FEDVNNLISIIEN--PNEAMIISTIVLVDIREFLVQRCVESGIHISNVLGPCISLVSTILNKTPEYKP 106

HSP 2 Score: 40.4318 bits (93), Expect = 2.321e-14
Identity = 17/43 (39.53%), Postives = 28/43 (65.12%), Query Frame = 1 
Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            + ++Y+++IEA+EF    DD      ++ ADI+L  +SRT KT
Sbjct:  112 MDKKYYKKIEAMEFAIRYDDSKDHSGIKHADIVLIGLSRTSKT 154

HSP 3 Score: 35.8094 bits (81), Expect = 2.321e-14
Identity = 17/37 (45.95%), Postives = 24/37 (64.86%), Query Frame = 3 
Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP-RVFSVDQKR 869
            K  LSIYLA KG K  N+P++  V  P  +F +D+K+
Sbjct:  153 KTPLSIYLANKGIKALNIPLMPEVPVPEELFEIDRKK 189
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y1185_STACT (Putative phosphotransferase Sca_1185 OS=Staphylococcus carnosus (strain TM300) GN=Sca_1185 PE=3 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 3.877e-14
Identity = 24/43 (55.81%), Postives = 29/43 (67.44%), Query Frame = 1 
Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L E YF++IEAIEF    DDG   + L KADI+L  +SRT KT
Sbjct:  117 LDEAYFKKIEAIEFAVKYDDGKDPKGLPKADIVLIGISRTSKT 159

HSP 2 Score: 35.4242 bits (80), Expect = 3.877e-14
Identity = 25/111 (22.52%), Postives = 49/111 (44.14%), Query Frame = 2 
Query:  272 MEGKSIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGA-MLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSP 601
            M+   I + SD  G TAE    A + QF      +N       +  I+ +E +  +I+ A ++   ++V+TL  P + +  ++      I   D++GP+   +   +   P
Sbjct:    1 MQKIKIIIASDSVGETAEQVAKACVSQFN----SKNFKSEIVRYPYIETIENVDEVIEIAKENELNIVVFTLVKPDIKQYMEERLAENKIKHVDIMGPLMSILTDKIDEQP 107

HSP 3 Score: 33.4982 bits (75), Expect = 3.877e-14
Identity = 17/37 (45.95%), Postives = 23/37 (62.16%), Query Frame = 3 
Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECPR-VFSVDQKR 869
            K  LS +LA K YKV N+PIV  +  P  +F +D K+
Sbjct:  158 KTPLSQFLAHKRYKVMNIPIVPEINPPEALFEIDPKK 194
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y1980_LISIN (Putative phosphotransferase lin1980 OS=Listeria innocua GN=lin1980 PE=3 SV=1)

HSP 1 Score: 46.9802 bits (110), Expect = 5.022e-14
Identity = 28/103 (27.18%), Postives = 51/103 (49.51%), Query Frame = 2 
Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGV 595
            IY++SD  G TA+H + A   QF    +++   +  H F  I D   L+  +++A     ++V TL    +A+ A   C    + + D+L  +T A+ +  G+
Sbjct:    7 IYVISDAIGETAQHIIRAVTAQFS---LNKPADIRRHAF--IRDENALLETLEEAKAADGIVVQTLVQSKLADYASNFCVKNHLQNVDLLHTLTAAVEAKTGL 104

HSP 2 Score: 40.0466 bits (92), Expect = 5.022e-14
Identity = 22/43 (51.16%), Postives = 24/43 (55.81%), Query Frame = 1 
Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L   YF RI AIEF    DD    + L  ADI+L  VSRT KT
Sbjct:  116 LDSNYFDRIAAIEFAVKYDDCKDPRGLLDADIVLVGVSRTSKT 158

HSP 3 Score: 31.5722 bits (70), Expect = 5.022e-14
Identity = 16/37 (43.24%), Postives = 23/37 (62.16%), Query Frame = 3 
Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP-RVFSVDQKR 869
            K  LS YLA + +KVANVP+V  +  P  +F +  +R
Sbjct:  157 KTPLSSYLANQNWKVANVPLVPEIPIPDELFQIPAER 193
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y606_MOOTA (Putative phosphotransferase Moth_0606 OS=Moorella thermoacetica (strain ATCC 39073) GN=Moth_0606 PE=3 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 5.361e-14
Identity = 35/123 (28.46%), Postives = 61/123 (49.59%), Query Frame = 2 
Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLPRGAPGRNFLLVRST 655
            IY++SD  G TAE+   AA  QF+         ++      + D++ L  ++ +AA++  ++ +TL  P + +   +     G+ + D+LGP+ +AI    G    G PR  PG    L+R T
Sbjct:   28 IYVISDSLGETAEYVARAAASQFD------GGGLDIRRVPYVTDLDHLEEVVNEAAQEQGIIAFTLVLPDLKKKLLELAAARGLEAVDLLGPLMDAITRVTG----GRPRLEPG----LIRRT 136

HSP 2 Score: 43.8986 bits (102), Expect = 5.361e-14
Identity = 20/41 (48.78%), Postives = 28/41 (68.29%), Query Frame = 1 
Query:  646 EEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            E+YFR++EAI+F    D+G   + L  AD++L  VSRT KT
Sbjct:  138 EDYFRKMEAIDFAVKYDNGKDIRGLSHADLVLIGVSRTSKT 178
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y2974_MARMM (Putative phosphotransferase Mmar10_2974 OS=Maricaulis maris (strain MCS10) GN=Mmar10_2974 PE=3 SV=1)

HSP 1 Score: 46.595 bits (109), Expect = 6.483e-14
Identity = 25/102 (24.51%), Postives = 51/102 (50.00%), Query Frame = 2 
Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLG 592
            I+MVSD TG T    + A++ QFE            HL++ +   +QL  ++ +      +++YT+ +  +    +  C   G+P+  +L P+   ++++LG
Sbjct:   14 IHMVSDSTGETLMEVMRASVAQFE------GTTPLEHLYALVRSSKQLDRVLDEVEAYPGVIMYTIVNADLRRELETRCVELGVPAIAILDPMLATLSAYLG 109

HSP 2 Score: 38.5058 bits (88), Expect = 6.483e-14
Identity = 19/43 (44.19%), Postives = 27/43 (62.79%), Query Frame = 1 
Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L  +Y+RRI+A+ +    DDG    + + ADI+L  VSRT KT
Sbjct:  122 LDADYYRRIDALNYAMAHDDG-QGDDFEGADIVLLGVSRTSKT 163

HSP 3 Score: 33.113 bits (74), Expect = 6.483e-14
Identity = 15/33 (45.45%), Postives = 22/33 (66.67%), Query Frame = 3 
Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECPR-VFSV 857
            K   S+YLA +G++ AN+P+V  V  P  VFS+
Sbjct:  162 KTPTSVYLAHRGFRAANIPLVKDVPIPEAVFSL 194
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y003_GRABC (Putative phosphotransferase GbCGDNIH1_0003 OS=Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) GN=GbCGDNIH1_0003 PE=3 SV=1)

HSP 1 Score: 45.8246 bits (107), Expect = 8.353e-14
Identity = 31/120 (25.83%), Postives = 52/120 (43.33%), Query Frame = 2 
Query:  284 SIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLPRGAPGRNFLL 643
            ++++VSD TG T      A + QFEH        +  H +S I    QL  +++    +   ++ TL +P +    +  C   GI    VL P+   +  H+G          PGR ++L
Sbjct:   21 NLHLVSDATGETLNSIARATVSQFEH------AQIIYHRWSLIRTRFQLHRVLEGIEAEPGPVLSTLIEPGLRSELENFCSRIGIAVVHVLDPVLSLLQHHIGEQAI----ARPGRQYVL 130

HSP 2 Score: 41.5874 bits (96), Expect = 8.353e-14
Identity = 19/46 (41.30%), Postives = 28/46 (60.87%), Query Frame = 1 
Query:  631 KFPLSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            ++ L  +YFRRI+A+ F    DDG     + +AD+ L  VSR+ KT
Sbjct:  127 QYVLDADYFRRIDAMHFVLAHDDGQAQVGINEADLCLVGVSRSSKT 172

HSP 3 Score: 30.4166 bits (67), Expect = 8.353e-14
Identity = 13/27 (48.15%), Postives = 17/27 (62.96%), Query Frame = 3 
Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP 842
            K   S YLA +G K AN+P+V G+  P
Sbjct:  171 KTPTSFYLANRGVKAANIPLVPGLPEP 197
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y001_CAUCR (Putative phosphotransferase CC_0001 OS=Caulobacter crescentus GN=CC_0001 PE=3 SV=1)

HSP 1 Score: 46.9802 bits (110), Expect = 1.077e-13
Identity = 22/43 (51.16%), Postives = 30/43 (69.77%), Query Frame = 1 
Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L+ +YF RIEA+++    DDG   Q+L +AD+IL  VSRT KT
Sbjct:  154 LTNDYFDRIEALDYAIAHDDGQGGQDLTQADVILVGVSRTSKT 196

HSP 2 Score: 42.3578 bits (98), Expect = 1.077e-13
Identity = 24/102 (23.53%), Postives = 48/102 (47.06%), Query Frame = 2 
Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLG 592
            I++VSD TG T      A   +F   L         H+++ +    QL   +++ A    ++++T+ DP +  + ++ C    +P    L P+  A++ +LG
Sbjct:   46 IHLVSDSTGETLNAMARAVCARFTDILPIE------HIYALVRSTRQLDRALEEIAGAPGVVMHTIVDPGLRAALEEGCRKLEMPCIAALDPVISAMSRYLG 141

HSP 3 Score: 28.1054 bits (61), Expect = 1.077e-13
Identity = 12/23 (52.17%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  762 KNTLSIYLAQKGYKVANVPIVMG 830
            K    IYLA +G + ANVP+V G
Sbjct:  195 KTPTCIYLAHRGVRAANVPLVPG 217
[Close]
BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y2242_CLOPE (Putative phosphotransferase CPE2242 OS=Clostridium perfringens GN=CPE2242 PE=3 SV=1)

HSP 1 Score: 42.3578 bits (98), Expect = 5.090e-13
Identity = 19/41 (46.34%), Postives = 27/41 (65.85%), Query Frame = 1 
Query:  646 EEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            E Y++RIEA+EF    DD    + L+ AD++L  +SRT KT
Sbjct:  114 ETYYKRIEAMEFAMQYDDSKDYRGLKNADVVLVGLSRTSKT 154

HSP 2 Score: 40.0466 bits (92), Expect = 5.090e-13
Identity = 32/110 (29.09%), Postives = 48/110 (43.64%), Query Frame = 2 
Query:  284 SIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLP 613
            +I+ VSD  G TA+    AA  QF+    D            ++DVE LM  I+    +  M+V T+   ++ E   + C    I   +VLGPI    ++ L   P   P
Sbjct:    3 TIFAVSDSIGETAQQVAMAAASQFK----DNAEVKRIPYVKSLEDVEDLMKTIEDC--EACMIVSTIITVNVREYLTQKCIEKNINIINVLGPIINVASTILNKYPDYNP 106

HSP 3 Score: 32.7278 bits (73), Expect = 5.090e-13
Identity = 15/39 (38.46%), Postives = 24/39 (61.54%), Query Frame = 3 
Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP-RVFSVDQKRFW 875
            K  L +YLA KG K  N+P+V  V  P  ++ +D+K+ +
Sbjct:  153 KTPLCMYLANKGIKAINIPLVPEVGVPEELYEIDKKKIF 191
The following BLAST results are available for this feature:
BLAST of EY692869 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 146
Match NameE-valueIdentityDescription
Y3095_CLOBM8.284e-1533.64Putative phosphotransferase CLK_3095 OS=Clostridiu... [more]
Y102_NITWN1.383e-1446.51Putative phosphotransferase Nwi_0102 OS=Nitrobacte... [more]
Y3950_CLOB62.321e-1432.73Putative phosphotransferase CLJ_B3950 OS=Clostridi... [more]
Y1185_STACT3.877e-1455.81Putative phosphotransferase Sca_1185 OS=Staphyloco... [more]
Y1980_LISIN5.022e-1427.18Putative phosphotransferase lin1980 OS=Listeria in... [more]
Y606_MOOTA5.361e-1428.46Putative phosphotransferase Moth_0606 OS=Moorella ... [more]
Y2974_MARMM6.483e-1424.51Putative phosphotransferase Mmar10_2974 OS=Maricau... [more]
Y003_GRABC8.353e-1425.83Putative phosphotransferase GbCGDNIH1_0003 OS=Gran... [more]
Y001_CAUCR1.077e-1351.16Putative phosphotransferase CC_0001 OS=Caulobacter... [more]
Y2242_CLOPE5.090e-1346.34Putative phosphotransferase CPE2242 OS=Clostridium... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-700-005-C11-CT.F Sweet orange fruit, development stadium (1 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY692869 ID=EY692869; Name=EY692869; organism=Citrus sinensis; type=EST; length=946bp
CCACGCGTCCGACCTCCCGAACCTCCACTCCACGCCGACCGCCCCCGGCC
AAACCACATCCGAGCCTGACTCTAAACCCCAGGCTCGGAAACTGAAGAGC
AGCCCTCAGCTGAACCGCTGGTCGAGGGCCCGCGCTGTACGATCAGGTCG
TAAATTGGACCGGTTGGGCCACCGGTCCCCCCAGTCGTCTGTTAATTTTA
AAGTTAGAGACAGAGATGGTGTTGATTTGGAGCCGGAAAGAATAAGTGAC
GGCGAGGATAACGTGGAGGCGATGGAAGGGAAGTCCATTTACATGGTCTC
TGACGGCACTGGATGGACGGCGGAGCACGCCGTTAATGCTGCGTTGGGGC
AGTTCGAGCACTGTTTAGTAGATCGAAACTGTGCTGTAAATACCCACCTC
TTCTCAGGGATTGATGATGTAGAGCAGTTGATGGTGATAATAAAGCAAGC
AGCCAAGGACGGGGCAATGCTTGTTTACACCTTAGCCGATCCTTCCATGG
CGGAGTCTGCTAAAAAGGCCTGCGAGCTTTGGGGTATACCGTCCACTGAC
GTATTGGGACCGATTACGGAGGCAATTGCATCCCATCTAGGTGTCTCACC
TTCTGGCCTTCCCCGCGGTGCTCCCGGCCGAAATTTCCTCTTAGTGAGGA
GTACTTTCGTCGCATAGAGGCTATTGAATTTACCATNNTAGCAGATGATG
GTGCCCCTTCACAAAATTTGCAAAAAGCCGACATTATTCTATCANGTGTT
TCTCGTACTGGAAAAACACGTTATCCATCTATTTAGCTCAGAAGGGATAC
AAAGTGGCAAATGTCCCAATTGTGATGGGTGTAGAATGCCCTAGAGTCTT
TTCAGTAGACCAGAAAAGGTTTTGGGNTTGACATNATCTCTAGTCTGCAT
CTATAAAAAGCAGAGCTAGAGTTGGGATTCGAGATGAATAGGAGCA
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