EY692869 | Citrus Genome Database

Overview


Name	EY692869
Unique Name	EY692869
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	946

Libraries


Library Name	Type
Sweet orange fruit, development stadium (1 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y1501_STAAS (Putative phosphotransferase SAS1501 OS=Staphylococcus aureus (strain MSSA476) GN=SAS1501 PE=3 SV=1)

HSP 1 Score: 48.9062 bits (115), Expect = 1.302e-17
Identity = 30/114 (26.32%), Postives = 52/114 (45.61%), Query Frame = 2

Query:  272 MEGKSIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLP 613
            ME   I + SD  G TAE    A + QF      + C      +  I+  E +  +I+ A    A++VYTL  P M +   +    + + S D++GP+ + +++ +   P   P
Sbjct:    1 MEKIKIIVASDSIGETAELVARAGISQFN----PKQCKNELLRYPYIESFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASVEEKPYNEP 110

HSP 2 Score: 47.7506 bits (112), Expect = 1.302e-17
Identity = 22/43 (51.16%), Postives = 29/43 (67.44%), Query Frame = 1

Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L + YF++I+AIEF    DDG   + L KADI+L  +SRT KT
Sbjct:  116 LDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKT 158

HSP 3 Score: 34.2686 bits (77), Expect = 1.302e-17
Identity = 19/37 (51.35%), Postives = 23/37 (62.16%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP-RVFSVDQKR 869
            K  LS YLA K YKV NVPIV  V  P  ++ +D K+
Sbjct:  157 KTPLSQYLAHKSYKVMNVPIVPEVTPPDGLYDIDPKK 193

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y1435_STAAB (Putative phosphotransferase SAB1435c OS=Staphylococcus aureus (strain bovine RF122 / ET3-1) GN=SAB1435c PE=3 SV=1)

HSP 1 Score: 48.9062 bits (115), Expect = 1.302e-17
Identity = 30/114 (26.32%), Postives = 52/114 (45.61%), Query Frame = 2

Query:  272 MEGKSIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLP 613
            ME   I + SD  G TAE    A + QF      + C      +  I+  E +  +I+ A    A++VYTL  P M +   +    + + S D++GP+ + +++ +   P   P
Sbjct:    1 MEKIKIIVASDSIGETAELVARAGISQFN----PKQCKNELLRYPYIESFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASVEEKPYNEP 110

HSP 2 Score: 47.7506 bits (112), Expect = 1.302e-17
Identity = 22/43 (51.16%), Postives = 29/43 (67.44%), Query Frame = 1

Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L + YF++I+AIEF    DDG   + L KADI+L  +SRT KT
Sbjct:  116 LDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKT 158

HSP 3 Score: 34.2686 bits (77), Expect = 1.302e-17
Identity = 19/37 (51.35%), Postives = 23/37 (62.16%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP-RVFSVDQKR 869
            K  LS YLA K YKV NVPIV  V  P  ++ +D K+
Sbjct:  157 KTPLSQYLAHKSYKVMNVPIVPEVTPPDGLYDIDPKK 193

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y1392_STAAN (Putative phosphotransferase SA1392 OS=Staphylococcus aureus (strain N315) GN=SA1392 PE=3 SV=1)

HSP 1 Score: 48.9062 bits (115), Expect = 1.302e-17
Identity = 30/114 (26.32%), Postives = 52/114 (45.61%), Query Frame = 2

Query:  272 MEGKSIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLP 613
            ME   I + SD  G TAE    A + QF      + C      +  I+  E +  +I+ A    A++VYTL  P M +   +    + + S D++GP+ + +++ +   P   P
Sbjct:    1 MEKIKIIVASDSIGETAELVARAGISQFN----PKQCKNELLRYPYIESFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASVEEKPYNEP 110

HSP 2 Score: 47.7506 bits (112), Expect = 1.302e-17
Identity = 22/43 (51.16%), Postives = 29/43 (67.44%), Query Frame = 1

Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L + YF++I+AIEF    DDG   + L KADI+L  +SRT KT
Sbjct:  116 LDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKT 158

HSP 3 Score: 34.2686 bits (77), Expect = 1.302e-17
Identity = 19/37 (51.35%), Postives = 23/37 (62.16%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP-RVFSVDQKR 869
            K  LS YLA K YKV NVPIV  V  P  ++ +D K+
Sbjct:  157 KTPLSQYLAHKSYKVMNVPIVPEVTPPDGLYDIDPKK 193

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: YSI3_STAAU (Putative phosphotransferase in Sigma70 operon OS=Staphylococcus aureus PE=3 SV=1)

HSP 1 Score: 48.9062 bits (115), Expect = 1.303e-17
Identity = 30/114 (26.32%), Postives = 52/114 (45.61%), Query Frame = 2

Query:  272 MEGKSIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLP 613
            ME   I + SD  G TAE    A + QF      + C      +  I+  E +  +I+ A    A++VYTL  P M +   +    + + S D++GP+ + +++ +   P   P
Sbjct:    1 MEKIKIIVASDSIGETAELVARAGISQFN----PKQCKNELLRYPYIESFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASVEEKPYNEP 110

HSP 2 Score: 47.7506 bits (112), Expect = 1.303e-17
Identity = 22/43 (51.16%), Postives = 29/43 (67.44%), Query Frame = 1

Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L + YF++I+AIEF    DDG   + L KADI+L  +SRT KT
Sbjct:  116 LDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKT 158

HSP 3 Score: 34.2686 bits (77), Expect = 1.303e-17
Identity = 19/37 (51.35%), Postives = 23/37 (62.16%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP-RVFSVDQKR 869
            K  LS YLA K YKV NVPIV  V  P  ++ +D K+
Sbjct:  157 KTPLSQYLAHKSYKVMNVPIVPEVTPPDGLYDIDPKK 193

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y3020_ALKMQ (Putative phosphotransferase Amet_3020 OS=Alkaliphilus metalliredigens (strain QYMF) GN=Amet_3020 PE=3 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 2.181e-17
Identity = 25/43 (58.14%), Postives = 31/43 (72.09%), Query Frame = 1

Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L E+YFR++EAIEF    DDG  S+ L KADI+L  +SRT KT
Sbjct:  116 LDEKYFRKVEAIEFAVKYDDGKDSRGLAKADIVLVGISRTSKT 158

HSP 2 Score: 45.0542 bits (105), Expect = 2.181e-17
Identity = 28/113 (24.78%), Postives = 51/113 (45.13%), Query Frame = 2

Query:  275 EGKSIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLP 613
            E   IY++SD  G TAE    AA+ QF+              F  I +   +  ++++A K+ +++V+T+    +     +  E   I S D++ P+  A+   L  +P   P
Sbjct:    4 ENLVIYILSDSIGETAEQVAKAAISQFD------TEDYEIRRFPFITEKHHIDEMLQEAKKENSVIVFTMVVEELRNYIMEEAEKMQIRSIDIMSPVLHAMGGALTTTPKREP 110

HSP 3 Score: 31.5722 bits (70), Expect = 2.181e-17
Identity = 15/28 (53.57%), Postives = 19/28 (67.86%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECPR 845
            K  LS+YLA +  KVANVP+V  V  P+
Sbjct:  157 KTPLSMYLAHRNMKVANVPLVPEVTPPK 184

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y1494_LISIN (Putative phosphotransferase lin1494 OS=Listeria innocua GN=lin1494 PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.182e-17
Identity = 32/114 (28.07%), Postives = 51/114 (44.74%), Query Frame = 2

Query:  272 MEGKSIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLP 613
            M   ++Y+VSD TG TAE    AAL QF             H F  +D    +  I+   A +  ++V+T+   S+ E   K  + +G+P  D+ GP+   +     + P   P
Sbjct:    1 MTQPAVYVVSDSTGETAELVTRAALSQFGQT------PKFIHRFHHVDSSHMIEEIVDLVAVNNGIIVHTIVLESVREELNKTAQAFGVPIIDLFGPLLNQLEETYKIKPLSEP 108

HSP 2 Score: 40.817 bits (94), Expect = 2.182e-17
Identity = 18/43 (41.86%), Postives = 26/43 (60.47%), Query Frame = 1

Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            + E YF ++ AIEF    DDG   + + +AD +L  +SRT KT
Sbjct:  114 MDEAYFNKVAAIEFAVENDDGRNPRGILQADYVLIGISRTSKT 156

HSP 3 Score: 34.6538 bits (78), Expect = 2.182e-17
Identity = 17/37 (45.95%), Postives = 23/37 (62.16%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP-RVFSVDQKR 869
            K  LS YLA KG K+ N+PIV   + P  +F +D K+
Sbjct:  155 KTPLSQYLALKGLKIVNIPIVPEAQIPDELFEIDPKK 191

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y1476_LISMF (Putative phosphotransferase LMOf2365_1476 OS=Listeria monocytogenes serotype 4b (strain F2365) GN=LMOf2365_1476 PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.182e-17
Identity = 32/114 (28.07%), Postives = 51/114 (44.74%), Query Frame = 2

Query:  272 MEGKSIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLP 613
            M   ++Y+VSD TG TAE    AAL QF             H F  +D    +  I+   A +  ++V+T+   S+ E   K  + +G+P  D+ GP+   +     + P   P
Sbjct:    1 MTQPAVYVVSDSTGETAELVTRAALSQFGQT------PKFIHRFHHVDSSHMIEEIVDLVAVNNGIIVHTIVLESVREELNKTAQAFGVPIIDLFGPLLNQLEETYKIKPLSEP 108

HSP 2 Score: 40.817 bits (94), Expect = 2.182e-17
Identity = 18/43 (41.86%), Postives = 26/43 (60.47%), Query Frame = 1

Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            + E YF ++ AIEF    DDG   + + +AD +L  +SRT KT
Sbjct:  114 MDEAYFNKVAAIEFAVENDDGRNPRGILQADYVLIGISRTSKT 156

HSP 3 Score: 34.6538 bits (78), Expect = 2.182e-17
Identity = 17/37 (45.95%), Postives = 23/37 (62.16%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP-RVFSVDQKR 869
            K  LS YLA KG K+ N+PIV   + P  +F +D K+
Sbjct:  155 KTPLSQYLALKGLKIVNIPIVPEAQIPDELFEIDPKK 191

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y1457_LISMO (Putative phosphotransferase lmo1457 OS=Listeria monocytogenes GN=lmo1457 PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.182e-17
Identity = 32/114 (28.07%), Postives = 51/114 (44.74%), Query Frame = 2

Query:  272 MEGKSIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSPSGLP 613
            M   ++Y+VSD TG TAE    AAL QF             H F  +D    +  I+   A +  ++V+T+   S+ E   K  + +G+P  D+ GP+   +     + P   P
Sbjct:    1 MTQPAVYVVSDSTGETAELVTRAALSQFGQT------PKFIHRFHHVDSSHMIEEIVDLVAVNNGIIVHTIVLESVREELNKTAQAFGVPIIDLFGPLLNQLEETYKIKPLSEP 108

HSP 2 Score: 40.817 bits (94), Expect = 2.182e-17
Identity = 18/43 (41.86%), Postives = 26/43 (60.47%), Query Frame = 1

Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            + E YF ++ AIEF    DDG   + + +AD +L  +SRT KT
Sbjct:  114 MDEAYFNKVAAIEFAVENDDGRNPRGILQADYVLIGISRTSKT 156

HSP 3 Score: 34.6538 bits (78), Expect = 2.182e-17
Identity = 17/37 (45.95%), Postives = 23/37 (62.16%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP-RVFSVDQKR 869
            K  LS YLA KG K+ N+PIV   + P  +F +D K+
Sbjct:  155 KTPLSQYLALKGLKIVNIPIVPEAQIPDELFEIDPKK 191

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y001_BARHE (Putative phosphotransferase BH00010 OS=Bartonella henselae GN=BH00010 PE=3 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 2.182e-17
Identity = 23/43 (53.49%), Postives = 30/43 (69.77%), Query Frame = 1

Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L+ +YFRRIEA++FT   DDG    +L  AD+IL  +SRT KT
Sbjct:  118 LNADYFRRIEALDFTIEHDDGQSPNSLSDADVILVGISRTSKT 160

HSP 2 Score: 46.2098 bits (108), Expect = 2.182e-17
Identity = 27/103 (26.21%), Postives = 48/103 (46.60%), Query Frame = 2

Query:  287 IYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGV 595
            ++M+SD TG T      A   Q+             H++  I +  QL   + +  ++  +++YT+ D  +    +K CE   IP  D+L P+  A  S+LG+
Sbjct:   10 LHMLSDATGETLISVGRAVASQYTMS------QATEHIYPMIRNKTQLQRALDEIQQEPGIVLYTIIDKKIKILLRKRCEKIEIPCIDILHPVLNAFKSYLGI 106

HSP 3 Score: 34.2686 bits (77), Expect = 2.182e-17
Identity = 14/27 (51.85%), Postives = 18/27 (66.67%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP 842
            K   SIYLA +G K ANVP++ G+  P
Sbjct:  159 KTPTSIYLANRGIKTANVPLIPGINLP 185

[Close]

BLAST of EY692869 vs. ExPASy Swiss-Prot
Match: Y2417_GEOSW (Putative phosphotransferase GWCH70_2417 OS=Geobacillus sp. (strain WCH70) GN=GWCH70_2417 PE=3 SV=1)

HSP 1 Score: 49.2914 bits (116), Expect = 2.832e-17
Identity = 23/43 (53.49%), Postives = 30/43 (69.77%), Query Frame = 1

Query:  640 LSEEYFRRIEAIEFTXXADDGAPSQNLQKADIILSXVSRTGKT 768
            L E+YF++IEAIEF    DDG   + + +ADI+L  VSRT KT
Sbjct:  114 LDEDYFKKIEAIEFAVKYDDGRDPRGILRADIVLIGVSRTSKT 156

HSP 2 Score: 47.3654 bits (111), Expect = 2.832e-17
Identity = 29/110 (26.36%), Postives = 52/110 (47.27%), Query Frame = 2

Query:  272 MEGKSIYMVSDGTGWTAEHAVNAALGQFEHCLVDRNCAVNTHLFSGIDDVEQLMVIIKQAAKDGAMLVYTLADPSMAESAKKACELWGIPSTDVLGPITEAIASHLGVSP 601
            M  + +Y+VSD  G TAE  V AA  QF       N  +       ++D   L  ++  A  + A++ +TL  P M +   +     G+ + D++GP+ E ++    ++P
Sbjct:    1 MNQRLVYVVSDSGGETAELVVRAAASQFY------NSHIQLKRVPYVEDKTMLAEVVALAKMNQAIIAFTLVVPEMRQFLIEEAAREGVVAYDIIGPLIEKMSDLFKLTP 104

HSP 3 Score: 33.113 bits (74), Expect = 2.832e-17
Identity = 20/37 (54.05%), Postives = 24/37 (64.86%), Query Frame = 3

Query:  762 KNTLSIYLAQKGYKVANVPIVMGVECP-RVFSVDQKR 869
            K  LS YLA K  KVANVPIV  VE P ++F V  ++
Sbjct:  155 KTPLSQYLAHKRLKVANVPIVPEVEPPEQLFKVPPEK 191

The following BLAST results are available for this feature:

BLAST of EY692869 vs. ExPASy Swiss-Prot

BLAST of EY692869 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 146


Match Name	E-value	Identity	Description
Y1501_STAAS	1.302e-17	26.32	Putative phosphotransferase SAS1501 OS=Staphylococ...	[more]
Y1435_STAAB	1.302e-17	26.32	Putative phosphotransferase SAB1435c OS=Staphyloco...	[more]
Y1392_STAAN	1.302e-17	26.32	Putative phosphotransferase SA1392 OS=Staphylococc...	[more]
YSI3_STAAU	1.303e-17	26.32	Putative phosphotransferase in Sigma70 operon OS=S...	[more]
Y3020_ALKMQ	2.181e-17	58.14	Putative phosphotransferase Amet_3020 OS=Alkaliphi...	[more]
Y1494_LISIN	2.182e-17	28.07	Putative phosphotransferase lin1494 OS=Listeria in...	[more]
Y1476_LISMF	2.182e-17	28.07	Putative phosphotransferase LMOf2365_1476 OS=Liste...	[more]
Y1457_LISMO	2.182e-17	28.07	Putative phosphotransferase lmo1457 OS=Listeria mo...	[more]
Y001_BARHE	2.182e-17	53.49	Putative phosphotransferase BH00010 OS=Bartonella ...	[more]
Y2417_GEOSW	2.832e-17	53.49	Putative phosphotransferase GWCH70_2417 OS=Geobaci...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-700-005-C11-CT.F Sweet orange fruit, development stadium (1 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY692869 ID=EY692869; Name=EY692869; organism=Citrus sinensis; type=EST; length=946bp
CCACGCGTCCGACCTCCCGAACCTCCACTCCACGCCGACCGCCCCCGGCC
AAACCACATCCGAGCCTGACTCTAAACCCCAGGCTCGGAAACTGAAGAGC
AGCCCTCAGCTGAACCGCTGGTCGAGGGCCCGCGCTGTACGATCAGGTCG
TAAATTGGACCGGTTGGGCCACCGGTCCCCCCAGTCGTCTGTTAATTTTA
AAGTTAGAGACAGAGATGGTGTTGATTTGGAGCCGGAAAGAATAAGTGAC
GGCGAGGATAACGTGGAGGCGATGGAAGGGAAGTCCATTTACATGGTCTC
TGACGGCACTGGATGGACGGCGGAGCACGCCGTTAATGCTGCGTTGGGGC
AGTTCGAGCACTGTTTAGTAGATCGAAACTGTGCTGTAAATACCCACCTC
TTCTCAGGGATTGATGATGTAGAGCAGTTGATGGTGATAATAAAGCAAGC
AGCCAAGGACGGGGCAATGCTTGTTTACACCTTAGCCGATCCTTCCATGG
CGGAGTCTGCTAAAAAGGCCTGCGAGCTTTGGGGTATACCGTCCACTGAC
GTATTGGGACCGATTACGGAGGCAATTGCATCCCATCTAGGTGTCTCACC
TTCTGGCCTTCCCCGCGGTGCTCCCGGCCGAAATTTCCTCTTAGTGAGGA
GTACTTTCGTCGCATAGAGGCTATTGAATTTACCATNNTAGCAGATGATG
GTGCCCCTTCACAAAATTTGCAAAAAGCCGACATTATTCTATCANGTGTT
TCTCGTACTGGAAAAACACGTTATCCATCTATTTAGCTCAGAAGGGATAC
AAAGTGGCAAATGTCCCAATTGTGATGGGTGTAGAATGCCCTAGAGTCTT
TTCAGTAGACCAGAAAAGGTTTTGGGNTTGACATNATCTCTAGTCTGCAT
CTATAAAAAGCAGAGCTAGAGTTGGGATTCGAGATGAATAGGAGCA