EY692485

Overview
NameEY692485
Unique NameEY692485
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length889
Libraries
Library NameType
Sweet orange fruit, development stadium (1 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACT5_XENLA (Actin, cytoplasmic type 5 OS=Xenopus laevis PE=3 SV=1)

HSP 1 Score: 289.656 bits (740), Expect = 1.768e-84
Identity = 137/168 (81.55%), Postives = 155/168 (92.26%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKL Y+ALD+ QE+ T+ +SS++EKSYEL DGQVITIG ERFRCPE LFQPS +GME+ GIHETT+NSIMKCDVDIRKDLY N VLSGG+TM+PGIADRM KEI+ALAPS+MKIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  209 IVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376

HSP 2 Score: 45.4394 bits (106), Expect = 1.768e-84
Identity = 30/48 (62.50%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P-H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAIL L L       Y  KILTERGYSFTTTAERE+
Sbjct:  162 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 209
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACT3_BOMMO (Actin, cytoplasmic A3 OS=Bombyx mori PE=3 SV=3)

HSP 1 Score: 289.656 bits (740), Expect = 1.768e-84
Identity = 138/168 (82.14%), Postives = 154/168 (91.67%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKL Y+ALD+ QE+ T+ +SS++EKSYEL DGQVITIG ERFRCPE LFQPS +GMEA GIHETTYNSIMKCDVDIRKDLY N VLSGG+TM+PGIADRM KEI+ LAPS+MKIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  209 IVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMEANGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITRLAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376

HSP 2 Score: 45.4394 bits (106), Expect = 1.768e-84
Identity = 30/48 (62.50%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P-H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAIL L L       Y  KILTERGYSFTTTAERE+
Sbjct:  162 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 209
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACT1_CAEEL (Actin-1/3 OS=Caenorhabditis elegans GN=act-1 PE=1 SV=2)

HSP 1 Score: 289.656 bits (740), Expect = 1.768e-84
Identity = 135/168 (80.36%), Postives = 156/168 (92.86%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKL Y+ALD+ QE+ T+ +SS++EKSYEL DGQVIT+G ERFRCPE +FQPS +GME+AGIHET+YNSIMKCD+DIRKDLY N VLSGG+TM+PGIADRM KEI+ALAPS+MKIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  209 IVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITVGNERFRCPEAMFQPSFLGMESAGIHETSYNSIMKCDIDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376

HSP 2 Score: 45.4394 bits (106), Expect = 1.768e-84
Identity = 30/48 (62.50%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P-H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAIL L L       Y  KILTERGYSFTTTAERE+
Sbjct:  162 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 209
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACTM_HELER (Actin, cytoskeletal OS=Heliocidaris erythrogramma PE=3 SV=1)

HSP 1 Score: 289.271 bits (739), Expect = 2.306e-84
Identity = 138/168 (82.14%), Postives = 154/168 (91.67%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKL Y ALD+ QE+ T+  SS++EKSYEL DGQVITIG ERFRCPE LFQP+ IGME+AGIHETTYNSIMKCD+DIRKDLY N VLSGG++M+PGIADRM KEI+ALAPSSMKIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  209 IVRDIKEKLCYSALDFEQEMATAAASSSLEKSYELPDGQVITIGNERFRCPETLFQPAFIGMESAGIHETTYNSIMKCDIDIRKDLYANTVLSGGTSMYPGIADRMQKEITALAPSSMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376

HSP 2 Score: 45.4394 bits (106), Expect = 2.306e-84
Identity = 30/48 (62.50%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P-H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAIL L L       Y  KILTERGYSFTTTAERE+
Sbjct:  162 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 209
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACT1_HELTB (Actin CyI, cytoplasmic OS=Heliocidaris tuberculata PE=3 SV=1)

HSP 1 Score: 289.271 bits (739), Expect = 2.306e-84
Identity = 139/168 (82.74%), Postives = 154/168 (91.67%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKL Y+ALD+ QE+ T+ +SS++EKSYEL DGQVITIG ERFR PE LFQPS +GME+AGIHETTYNSIMKCDVDIRKDLY N VLSGGSTMFPGIADRM KEI+ALAP +MKIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  209 IVRDIKEKLCYVALDFEQEMSTAASSSSLEKSYELPDGQVITIGNERFRAPESLFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYANTVLSGGSTMFPGIADRMQKEITALAPPTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376

HSP 2 Score: 45.4394 bits (106), Expect = 2.306e-84
Identity = 30/48 (62.50%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P-H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAIL L L       Y  KILTERGYSFTTTAERE+
Sbjct:  162 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 209
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACT1_HELER (Actin CyI, cytoplasmic OS=Heliocidaris erythrogramma PE=3 SV=1)

HSP 1 Score: 289.271 bits (739), Expect = 2.306e-84
Identity = 139/168 (82.74%), Postives = 154/168 (91.67%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKL Y+ALD+ QE+ T+ +SS++EKSYEL DGQVITIG ERFR PE LFQPS +GME+AGIHETTYNSIMKCDVDIRKDLY N VLSGGSTMFPGIADRM KEI+ALAP +MKIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  209 IVRDIKEKLCYVALDFEQEMSTAASSSSLEKSYELPDGQVITIGNERFRAPESLFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYANTVLSGGSTMFPGIADRMQKEITALAPPTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376

HSP 2 Score: 45.4394 bits (106), Expect = 2.306e-84
Identity = 30/48 (62.50%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P-H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAIL L L       Y  KILTERGYSFTTTAERE+
Sbjct:  162 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 209
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACT23_DICDI (Putative actin-23 OS=Dictyostelium discoideum GN=act23 PE=1 SV=1)

HSP 1 Score: 289.271 bits (739), Expect = 2.307e-84
Identity = 141/168 (83.93%), Postives = 159/168 (94.64%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKLAY+ALD+  E++T+ TSSA+EKSYEL DGQVITIG ERFRCPE LFQPS +GME+AGIHETTYNSIMKCDVDIRKDLYGN+VLSGG+TMFPGIADRM+KE++ALAPS+MKIK++APPERKYSVWI GSILASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  193 IVRDIKEKLAYVALDFEAEMQTA-TSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWI-GSILASLSTFQQMWISKEEYDESGPSIVHRKCF 358

HSP 2 Score: 45.4394 bits (106), Expect = 2.307e-84
Identity = 30/48 (62.50%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P-H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAIL L L       Y  KILTERGYSFTTTAERE+
Sbjct:  146 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 193
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACT5_CHICK (Actin, cytoplasmic type 5 OS=Gallus gallus PE=3 SV=1)

HSP 1 Score: 288.886 bits (738), Expect = 3.008e-84
Identity = 136/168 (80.95%), Postives = 155/168 (92.26%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKL Y+ALD+ QE+ T+ +SS++EKSYEL DGQVITIG ERFRCPE +FQPS +GME+ GIHETT+NSIMKCDVDIRKDLY N VLSGG+TM+PGIADRM KEI+ALAPS+MKIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  209 IVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEAIFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376

HSP 2 Score: 45.4394 bits (106), Expect = 3.008e-84
Identity = 30/48 (62.50%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P-H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAIL L L       Y  KILTERGYSFTTTAERE+
Sbjct:  162 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 209
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACTB_CRIGR (Actin, cytoplasmic 1 OS=Cricetulus griseus GN=ACTB PE=3 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 3.923e-84
Identity = 136/168 (80.95%), Postives = 155/168 (92.26%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKL Y+ALD+ QE+ T+ +SS++EKSYEL DGQVITIG ERFRCPE LFQPS +GME+ GIHETT+NSIMKCDVDIRKDLY N VLSGG+TM+PGIADRM KEI+ALAPS+MKIK++APPERKYSVWIGGSILASLSTFQ+MWI+K EYDESGPSIVHRKCF
Sbjct:  208 IVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQEMWISKQEYDESGPSIVHRKCF 375

HSP 2 Score: 45.4394 bits (106), Expect = 3.923e-84
Identity = 30/48 (62.50%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P-H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAIL L L       Y  KILTERGYSFTTTAERE+
Sbjct:  161 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 208
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACT1_PODCA (Actin-1/2 OS=Podocoryne carnea GN=ACTIA PE=2 SV=1)

HSP 1 Score: 288.886 bits (738), Expect = 5.117e-84
Identity = 138/168 (82.14%), Postives = 156/168 (92.86%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKLAY+ALD+ QE++T+ +SS++EKSYEL DGQVITIG ERFR PE LFQP+ +GME+AGIHETTYNSIMKCDVDIRKDLY N VLSGG+TMFPGIADRM KEIS+LAP +MKIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  209 IVRDIKEKLAYVALDFEQEMQTAASSSSLEKSYELPDGQVITIGNERFRDPEALFQPAFLGMESAGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMFPGIADRMQKEISSLAPPTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376

HSP 2 Score: 44.669 bits (104), Expect = 5.117e-84
Identity = 29/48 (60.42%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P-H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAI+ L L       Y  KILTERGYSFTTTAERE+
Sbjct:  162 HTVPIYEGYALPHAIIRLDLAGRDLTDYMMKILTERGYSFTTTAEREI 209
The following BLAST results are available for this feature:
BLAST of EY692485 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 479
Match NameE-valueIdentityDescription
ACT5_XENLA1.768e-8481.55Actin, cytoplasmic type 5 OS=Xenopus laevis PE=3 S... [more]
ACT3_BOMMO1.768e-8482.14Actin, cytoplasmic A3 OS=Bombyx mori PE=3 SV=3[more]
ACT1_CAEEL1.768e-8480.36Actin-1/3 OS=Caenorhabditis elegans GN=act-1 PE=1 ... [more]
ACTM_HELER2.306e-8482.14Actin, cytoskeletal OS=Heliocidaris erythrogramma ... [more]
ACT1_HELTB2.306e-8482.74Actin CyI, cytoplasmic OS=Heliocidaris tuberculata... [more]
ACT1_HELER2.306e-8482.74Actin CyI, cytoplasmic OS=Heliocidaris erythrogram... [more]
ACT23_DICDI2.307e-8483.93Putative actin-23 OS=Dictyostelium discoideum GN=a... [more]
ACT5_CHICK3.008e-8480.95Actin, cytoplasmic type 5 OS=Gallus gallus PE=3 SV... [more]
ACTB_CRIGR3.923e-8480.95Actin, cytoplasmic 1 OS=Cricetulus griseus GN=ACTB... [more]
ACT1_PODCA5.117e-8482.14Actin-1/2 OS=Podocoryne carnea GN=ACTIA PE=2 SV=1[more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-700-001-B08-CT.F Sweet orange fruit, development stadium (1 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY692485 ID=EY692485; Name=EY692485; organism=Citrus sinensis; type=EST; length=889bp
CCACGCGTCCGTTTTTTTTTTTCAAAAAACTTCTCATAATAGATGAGAAT
GAAAGAACTTGACCATTTTGAAAATCAACTCAGCCTTCTCAAAGTCATCG
CTCCAACACGAAAATAAACACATGGATAACAAAGCTAAAATGAAAAAAGG
TTGCAAACTGCAATTGGAAAACAGTAATTCTTTCAAAAATCCTATTGGTG
AACAGTTTAGAAGCACTTTCTATGCACAATTGATGGCCCGGACTCGTCAT
ACTCAGCCTTTGCAATCCACATCTGCTGGAATGTGCTAAGAGAAGCCAAG
ATGGAGCCTCCTATCCAGACACTATACTTCCTCTCAGGTGGTGCCACTAC
CTTAATCTTCATGCTGCTTGGGGCTAGGGCAGAAATTTCCTTGCTCATTC
TATCGGCAATGCCAGGGAACATAGTGGAACCACCACTGAGGACAATGTTG
CCGTACAGGTCTTTTCTGATATCAACATCGCACTTCATGATGGAGTTGTA
AGTGGTCTCGTGGATGCCTGCTGCTTCCATACCAATCATGGATGGTTGGA
AAAGGACCTCAGGACACCTGAAACGCTCAGCGCCAATAGTGATAACCTGC
CCATCANGCAACTCATAGCTCTTCTCAACTGCAGAGCTGGTCTTGGAAGT
CTCAAGCTCCTGNTCATAGTCAAGAGCGATGTAAGCCAGCTTTTCCTTCA
TGTCCCTGACAATTCACGCTCCGCCGTGGTGGTGAAAGAGTACCCACGCT
CAGTGAGGATTTTTCATCAGTATCAGTGAGGTCACGACAGCAAGGTCAAG
ACGAGTATTGCATGTGGGAGGCATACCCTCATATATAAGACTGTATGCTG
ACACATCACAGAGTCAACCCATTACAGGGTACGACCTCC
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