EY692485

Overview
NameEY692485
Unique NameEY692485
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length889
Libraries
Library NameType
Sweet orange fruit, development stadium (1 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACT1_MAIZE (Actin-1 OS=Zea mays GN=ACT1 PE=3 SV=1)

HSP 1 Score: 296.59 bits (758), Expect = 1.039e-84
Identity = 140/168 (83.33%), Postives = 157/168 (93.45%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKLAY+ALDY QELET+K+SS+VEKSYE+ DGQVITIG+ERFRCPEVLFQPS++GME+  +HE TYNSIMKCDVDIRKDLYGN+VLSGG TMFPGIADRMSKEI++L PSSMK+KVVAPP RKYSVWIGGSILASLSTFQQMWI+K EYDE+GP IVH KCF
Sbjct:  208 IVRDIKEKLAYVALDYEQELETAKSSSSVEKSYEMPDGQVITIGSERFRCPEVLFQPSLVGMESPSVHEATYNSIMKCDVDIRKDLYGNVVLSGGFTMFPGIADRMSKEITSLVPSSMKVKVVAPPRRKYSVWIGGSILASLSTFQQMWISKGEYDETGPGIVHMKCF 375

HSP 2 Score: 39.2762 bits (90), Expect = 1.039e-84
Identity = 28/50 (56.00%), Postives = 31/50 (62.00%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P---H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAIL L L       H    KILTERGYS TT+AERE+
Sbjct:  161 HTVPIYEGYTLPHAILRLDLAGRDLTDHLM--KILTERGYSLTTSAEREI 208
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACTM_APLCA (Actin, muscle OS=Aplysia californica PE=2 SV=1)

HSP 1 Score: 290.426 bits (742), Expect = 1.039e-84
Identity = 138/168 (82.14%), Postives = 156/168 (92.86%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKL Y+ALD+ QE+ T+ +SS++EKSYEL DGQVITIG ERFRCPE LFQP ++GME+AGIHETTYNSIMKCDVDIRKDLY N VLSGG+TMFPGIADRM KEI++LAPS+MKIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  209 IVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPESLFQPILLGMESAGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMFPGIADRMQKEITSLAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376

HSP 2 Score: 45.4394 bits (106), Expect = 1.039e-84
Identity = 30/48 (62.50%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P-H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAIL L L       Y  KILTERGYSFTTTAERE+
Sbjct:  162 HTVPIYEGYALPHAILRLDLAGRDLTDYLSKILTERGYSFTTTAEREI 209
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACT4_CAEEL (Actin-4 OS=Caenorhabditis elegans GN=act-4 PE=2 SV=2)

HSP 1 Score: 290.426 bits (742), Expect = 1.039e-84
Identity = 136/168 (80.95%), Postives = 156/168 (92.86%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKL Y+ALD+ QE+ T+ +SS++EKSYEL DGQVIT+G ERFRCPE LFQPS +GME+AGIHET+YNSIMKCD+DIRKDLY N VLSGG+TM+PGIADRM KEI+ALAPS+MKIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  209 IVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITVGNERFRCPEALFQPSFLGMESAGIHETSYNSIMKCDIDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376

HSP 2 Score: 45.4394 bits (106), Expect = 1.039e-84
Identity = 30/48 (62.50%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P-H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAIL L L       Y  KILTERGYSFTTTAERE+
Sbjct:  162 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 209
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACT2_CAEEL (Actin-2 OS=Caenorhabditis elegans GN=act-2 PE=2 SV=3)

HSP 1 Score: 290.426 bits (742), Expect = 1.039e-84
Identity = 136/168 (80.95%), Postives = 156/168 (92.86%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKL Y+ALD+ QE+ T+ +SS++EKSYEL DGQVIT+G ERFRCPE LFQPS +GME+AGIHET+YNSIMKCD+DIRKDLY N VLSGG+TM+PGIADRM KEI+ALAPS+MKIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  209 IVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITVGNERFRCPEALFQPSFLGMESAGIHETSYNSIMKCDIDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376

HSP 2 Score: 45.4394 bits (106), Expect = 1.039e-84
Identity = 30/48 (62.50%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P-H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAIL L L       Y  KILTERGYSFTTTAERE+
Sbjct:  162 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 209
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACT1_ONCVO (Actin-1 OS=Onchocerca volvulus GN=act-1a PE=3 SV=1)

HSP 1 Score: 290.426 bits (742), Expect = 1.039e-84
Identity = 135/168 (80.36%), Postives = 157/168 (93.45%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKL Y+ALD+ QE+ T+ +SS++EKSYEL DGQVIT+G ERFRCPE LFQPS +GME+AGIHE+TYNSIMKCD+DIRKDLY NIVLSGG+TM+PGIADRM KE++ALAPS+MKIK++APPERKYSVWIGGSILASLSTFQQ+WI+K EYDESGPSIVHRKCF
Sbjct:  209 IVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITVGNERFRCPEALFQPSFLGMESAGIHESTYNSIMKCDIDIRKDLYANIVLSGGTTMYPGIADRMQKEVTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQVWISKQEYDESGPSIVHRKCF 376

HSP 2 Score: 45.4394 bits (106), Expect = 1.039e-84
Identity = 30/48 (62.50%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P-H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAIL L L       Y  KILTERGYSFTTTAERE+
Sbjct:  162 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 209
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACTA_LIMPO (Actin, acrosomal process isoform OS=Limulus polyphemus PE=2 SV=1)

HSP 1 Score: 290.041 bits (741), Expect = 1.355e-84
Identity = 138/168 (82.14%), Postives = 153/168 (91.07%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKL Y+ALD+  E+ T+ +SS++EKSYEL DGQVITIG ERFRCPE +FQPS +GMEA GIHETT+NSIMKCDVDIRKDLY N VLSGGSTMFPGIADRM KEI ALAPS+MKIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  209 IVRDIKEKLCYVALDFEHEMTTAASSSSLEKSYELPDGQVITIGNERFRCPEAMFQPSFLGMEACGIHETTFNSIMKCDVDIRKDLYANTVLSGGSTMFPGIADRMQKEIGALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376

HSP 2 Score: 45.4394 bits (106), Expect = 1.355e-84
Identity = 30/48 (62.50%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P-H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAIL L L       Y  KILTERGYSFTTTAERE+
Sbjct:  162 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 209
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACT4_ARTSX (Actin, clone 403 OS=Artemia sp. PE=2 SV=1)

HSP 1 Score: 290.041 bits (741), Expect = 1.355e-84
Identity = 137/168 (81.55%), Postives = 155/168 (92.26%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKL Y+ALD+ QE+ T+ +S+++EKSYEL DGQVITIG ERFRCPE LFQPS +GME+ GIHETTYNSIMKCDVDIRKDLY N VLSGG+TM+PGIADRM KEI+ALAPS+MKIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  209 IVRDIKEKLCYVALDFEQEMATAASSTSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTYNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376

HSP 2 Score: 45.4394 bits (106), Expect = 1.355e-84
Identity = 30/48 (62.50%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P-H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAIL L L       Y  KILTERGYSFTTTAERE+
Sbjct:  162 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 209
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACT_CRAGI (Actin OS=Crassostrea gigas PE=2 SV=1)

HSP 1 Score: 290.426 bits (742), Expect = 1.768e-84
Identity = 137/168 (81.55%), Postives = 156/168 (92.86%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKL Y+ALD+ QE+ T+ +SS++EKSYEL DGQVITIG ERFRCPE +FQPS +GME++GIHET+YNSIMKCDVDIRKDLY NIVLSGG+TMFPGIADRM KE++ALAP +MKIKV+APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  209 IVRDIKEKLCYVALDFEQEMTTAASSSSLEKSYELPDGQVITIGNERFRCPEAMFQPSFLGMESSGIHETSYNSIMKCDVDIRKDLYANIVLSGGTTMFPGIADRMQKEVTALAPPTMKIKVIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376

HSP 2 Score: 44.669 bits (104), Expect = 1.768e-84
Identity = 29/48 (60.42%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P-H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAI+ L L       Y  KILTERGYSFTTTAERE+
Sbjct:  162 HTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 209
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACTM_HELTB (Actin, cytoskeletal OS=Heliocidaris tuberculata PE=3 SV=1)

HSP 1 Score: 289.656 bits (740), Expect = 1.768e-84
Identity = 138/168 (82.14%), Postives = 154/168 (91.67%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKL Y ALD+ QE+ T+  SS++EKSYEL DGQVITIG ERFRCPE LFQP+ IGME+AGIHETTYNSIMKCD+DIRKDLY N VLSGG++M+PGIADRM KEI+ALAPSSMKIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  209 IVRDIKEKLCYTALDFEQEMATAAASSSLEKSYELPDGQVITIGNERFRCPETLFQPAFIGMESAGIHETTYNSIMKCDIDIRKDLYANTVLSGGTSMYPGIADRMQKEITALAPSSMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 376

HSP 2 Score: 45.4394 bits (106), Expect = 1.768e-84
Identity = 30/48 (62.50%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P-H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAIL L L       Y  KILTERGYSFTTTAERE+
Sbjct:  162 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 209
[Close]
BLAST of EY692485 vs. ExPASy Swiss-Prot
Match: ACTG_XENTR (Actin, cytoplasmic 2 OS=Xenopus tropicalis GN=actg1 PE=2 SV=1)

HSP 1 Score: 289.656 bits (740), Expect = 1.768e-84
Identity = 137/168 (81.55%), Postives = 155/168 (92.26%), Query Frame = -3 
Query:  210 IVRDMKEKLAYIALDYXQELETSKTSSAVEKSYELXDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEISALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF 713
            IVRD+KEKL Y+ALD+ QE+ T+ +SS++EKSYEL DGQVITIG ERFRCPE LFQPS +GME+ GIHETT+NSIMKCDVDIRKDLY N VLSGG+TM+PGIADRM KEI+ALAPS+MKIK++APPERKYSVWIGGSILASLSTFQQMWI+K EYDESGPSIVHRKCF
Sbjct:  208 IVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 375

HSP 2 Score: 45.4394 bits (106), Expect = 1.768e-84
Identity = 30/48 (62.50%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  710 HTV-LYMRVCLPHAILVLTLLS*P-H*Y**KILTERGYSFTTTAEREL 847
            HTV +Y    LPHAIL L L       Y  KILTERGYSFTTTAERE+
Sbjct:  161 HTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREI 208
The following BLAST results are available for this feature:
BLAST of EY692485 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 479
Match NameE-valueIdentityDescription
ACT1_MAIZE1.039e-8483.33Actin-1 OS=Zea mays GN=ACT1 PE=3 SV=1[more]
ACTM_APLCA1.039e-8482.14Actin, muscle OS=Aplysia californica PE=2 SV=1[more]
ACT4_CAEEL1.039e-8480.95Actin-4 OS=Caenorhabditis elegans GN=act-4 PE=2 SV... [more]
ACT2_CAEEL1.039e-8480.95Actin-2 OS=Caenorhabditis elegans GN=act-2 PE=2 SV... [more]
ACT1_ONCVO1.039e-8480.36Actin-1 OS=Onchocerca volvulus GN=act-1a PE=3 SV=1[more]
ACTA_LIMPO1.355e-8482.14Actin, acrosomal process isoform OS=Limulus polyph... [more]
ACT4_ARTSX1.355e-8481.55Actin, clone 403 OS=Artemia sp. PE=2 SV=1[more]
ACT_CRAGI1.768e-8481.55Actin OS=Crassostrea gigas PE=2 SV=1[more]
ACTM_HELTB1.768e-8482.14Actin, cytoskeletal OS=Heliocidaris tuberculata PE... [more]
ACTG_XENTR1.768e-8481.55Actin, cytoplasmic 2 OS=Xenopus tropicalis GN=actg... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-700-001-B08-CT.F Sweet orange fruit, development stadium (1 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY692485 ID=EY692485; Name=EY692485; organism=Citrus sinensis; type=EST; length=889bp
CCACGCGTCCGTTTTTTTTTTTCAAAAAACTTCTCATAATAGATGAGAAT
GAAAGAACTTGACCATTTTGAAAATCAACTCAGCCTTCTCAAAGTCATCG
CTCCAACACGAAAATAAACACATGGATAACAAAGCTAAAATGAAAAAAGG
TTGCAAACTGCAATTGGAAAACAGTAATTCTTTCAAAAATCCTATTGGTG
AACAGTTTAGAAGCACTTTCTATGCACAATTGATGGCCCGGACTCGTCAT
ACTCAGCCTTTGCAATCCACATCTGCTGGAATGTGCTAAGAGAAGCCAAG
ATGGAGCCTCCTATCCAGACACTATACTTCCTCTCAGGTGGTGCCACTAC
CTTAATCTTCATGCTGCTTGGGGCTAGGGCAGAAATTTCCTTGCTCATTC
TATCGGCAATGCCAGGGAACATAGTGGAACCACCACTGAGGACAATGTTG
CCGTACAGGTCTTTTCTGATATCAACATCGCACTTCATGATGGAGTTGTA
AGTGGTCTCGTGGATGCCTGCTGCTTCCATACCAATCATGGATGGTTGGA
AAAGGACCTCAGGACACCTGAAACGCTCAGCGCCAATAGTGATAACCTGC
CCATCANGCAACTCATAGCTCTTCTCAACTGCAGAGCTGGTCTTGGAAGT
CTCAAGCTCCTGNTCATAGTCAAGAGCGATGTAAGCCAGCTTTTCCTTCA
TGTCCCTGACAATTCACGCTCCGCCGTGGTGGTGAAAGAGTACCCACGCT
CAGTGAGGATTTTTCATCAGTATCAGTGAGGTCACGACAGCAAGGTCAAG
ACGAGTATTGCATGTGGGAGGCATACCCTCATATATAAGACTGTATGCTG
ACACATCACAGAGTCAACCCATTACAGGGTACGACCTCC
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