EY692471

Overview
NameEY692471
Unique NameEY692471
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length892
Libraries
Library NameType
Sweet orange fruit, development stadium (1 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY692471 vs. ExPASy Swiss-Prot
Match: 7OMT9_MEDSA (Isoflavone-7-O-methyltransferase 9 OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 4.130e-18
Identity = 60/248 (24.19%), Postives = 109/248 (43.95%), Query Frame = 1 
Query:   43 KSKSKVTNEEEEVYSHAMVLGSAIVLPAVFQAVVNLDVLDIINKAGPGAELSASEIASQIPTKNPEGAAKMLDRVLGLLVSFNILCCSFSTHGVRLYSLAPVAKYYVSDQNGVSMRPYMNLSLDKVMMPGWFQLKDQILEGELS-YNKALGMDFYDYLGTDSRFNEVFNNGMIGQTSFVINKIVESYKGFQNITRLVDVGGGLXITLATIISKYPHIKGINFDVPHVIKDASSYPGMEHXGRDMYKKI 783
            +  S++   +  +Y H      ++ L    +  V +++ +II+  G    LS      Q+P+    G  + L R L     F I+     T     Y+L   ++  V   + + + P +   LD  +   + +LK  I E +L+ +   LG  F+D+L  +  +N  FN+ M   +  +   + +    F  +  +VDVGGG   T   I   +P +K I FD P V+++ S    + + G DM+  I
Sbjct:    8 RKPSEIFKAQALLYKHIYAFIDSMSL----KWAVGMNIPNIIHNHGKPISLSNLVSILQVPSSKI-GNVRRLMRYLAHNGFFEII-----TKEEESYALTVASELLVRGSD-LCLAPMVECVLDPTLSGSYHELKKWIYEEDLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDSKLINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNLTYVGGDMFTSI 244

HSP 2 Score: 38.1206 bits (87), Expect = 4.130e-18
Identity = 16/36 (44.44%), Postives = 28/36 (77.78%), Query Frame = 3 
Query:  783 PEGDAILMKGMLHNFDDENCIKILRIVTKS-SNDGK 887
            P  DA+L+K +LHN+ D++C++IL+   ++ +NDGK
Sbjct:  245 PNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGK 280
[Close]
BLAST of EY692471 vs. ExPASy Swiss-Prot
Match: 7OMT8_MEDSA (Isoflavone-7-O-methyltransferase 8 OS=Medicago sativa PE=1 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 5.370e-18
Identity = 60/248 (24.19%), Postives = 108/248 (43.55%), Query Frame = 1 
Query:   43 KSKSKVTNEEEEVYSHAMVLGSAIVLPAVFQAVVNLDVLDIINKAGPGAELSASEIASQIPTKNPEGAAKMLDRVLGLLVSFNILCCSFSTHGVRLYSLAPVAKYYVSDQNGVSMRPYMNLSLDKVMMPGWFQLKDQILEGELS-YNKALGMDFYDYLGTDSRFNEVFNNGMIGQTSFVINKIVESYKGFQNITRLVDVGGGLXITLATIISKYPHIKGINFDVPHVIKDASSYPGMEHXGRDMYKKI 783
            +  S++   +  +Y H      ++ L    +  V +++ +II   G    LS      Q+P+    G  + L R L     F I+     T     Y+L   ++  V   + + + P +   LD  +   + +LK  I E +L+ +   LG  F+D+L  +  +N  FN+ M   +  +   + +    F  +  +VDVGGG   T   I   +P +K I FD P V+++ S    + + G DM+  I
Sbjct:    8 RKPSEIFKAQALLYKHIYAFIDSMSL----KWAVEMNIPNIIQNHGKPISLSNLVSILQVPSSKI-GNVRRLMRYLAHNGFFEII-----TKEEESYALTVASELLVRGSD-LCLAPMVECVLDPTLSGSYHELKKWIYEEDLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDSKLINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNLTYVGGDMFTSI 244

HSP 2 Score: 38.1206 bits (87), Expect = 5.370e-18
Identity = 16/36 (44.44%), Postives = 28/36 (77.78%), Query Frame = 3 
Query:  783 PEGDAILMKGMLHNFDDENCIKILRIVTKS-SNDGK 887
            P  DA+L+K +LHN+ D++C++IL+   ++ +NDGK
Sbjct:  245 PNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGK 280
[Close]
BLAST of EY692471 vs. ExPASy Swiss-Prot
Match: 7OMT6_MEDSA (Isoflavone-7-O-methyltransferase 6 OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 5.370e-18
Identity = 60/248 (24.19%), Postives = 108/248 (43.55%), Query Frame = 1 
Query:   43 KSKSKVTNEEEEVYSHAMVLGSAIVLPAVFQAVVNLDVLDIINKAGPGAELSASEIASQIPTKNPEGAAKMLDRVLGLLVSFNILCCSFSTHGVRLYSLAPVAKYYVSDQNGVSMRPYMNLSLDKVMMPGWFQLKDQILEGELS-YNKALGMDFYDYLGTDSRFNEVFNNGMIGQTSFVINKIVESYKGFQNITRLVDVGGGLXITLATIISKYPHIKGINFDVPHVIKDASSYPGMEHXGRDMYKKI 783
            +  S++   +  +Y H      ++ L    +  V +++ +II   G    LS      Q+P+    G  + L R L     F I+     T     Y+L   ++  V   + + + P +   LD  +   + +LK  I E +L+ +   LG  F+D+L  +  +N  FN+ M   +  +   + +    F  +  +VDVGGG   T   I   +P +K I FD P V+++ S    + + G DM+  I
Sbjct:    8 RKPSEIFKAQALLYKHIYAFIDSMSL----KWAVEMNIPNIIQNHGKPISLSNLVSILQVPSSKI-GNVRRLMRYLAHNGFFEII-----TKEEESYALTVASELLVRGSD-LCLAPMVECVLDPTLSGSYHELKKWIYEEDLTLFGVTLGSGFWDFLDKNPEYNTSFNDAMASDSKLINLALRDCDFVFDGLESIVDVGGGTGTTAKIICETFPKLKCIVFDRPQVVENLSGSNNLTYVGGDMFTSI 244

HSP 2 Score: 38.1206 bits (87), Expect = 5.370e-18
Identity = 16/36 (44.44%), Postives = 28/36 (77.78%), Query Frame = 3 
Query:  783 PEGDAILMKGMLHNFDDENCIKILRIVTKS-SNDGK 887
            P  DA+L+K +LHN+ D++C++IL+   ++ +NDGK
Sbjct:  245 PNADAVLLKYILHNWTDKDCLRILKKCKEAVTNDGK 280
[Close]
BLAST of EY692471 vs. ExPASy Swiss-Prot
Match: ZRP4_MAIZE (O-methyltransferase ZRP4 OS=Zea mays GN=ZRP4 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 3.571e-16
Identity = 55/238 (23.11%), Postives = 108/238 (45.38%), Query Frame = 1 
Query:  109 AIVLPAVFQAVVNLDVLDIINKAGPGAELSASEIASQIPTKNPEGAAKMLDRVLGLLVSFNI-----LCCSFSTHGVRLYSLAPVAKYYVSDQNG-VSMRPYMNLSLDKVMMP------GWFQLKDQILEGELSYNKALGMDFYDYLGTDSRFNEVFNNGMIGQTSFVIN-KIVESYKGFQNITRLVDVGGGLXITLATIISKYPHIKGINFDVPHVIKDASSYPGMEHXGRDMYKKI 783
            A +     ++ ++L + D I+  G  A LS  +I S++           L R++ +L + N+     L          +Y+L PV++  +  Q+  ++  P   + LD  ++        WFQ +   L     +    G   ++    D+ F+ + N+G+   +  +++  I +S + FQ I+ LVDVGGG+      I   +PH+K    D+ HV+  A ++  ++    DM++ I
Sbjct:   26 AFMKSMALKSAIHLRIADAIHLHGGAASLS--QILSKVHLH--PSRVSSLRRLMRVLTTTNVFGTQPLGGGSDDDSEPVYTLTPVSRLLIGSQSSQLAQTPLAAMVLDPTIVSPFSELGAWFQHE---LPDPCIFKHTHGRGIWELTKDDATFDALVNDGLASDSQLIVDVAIKQSAEVFQGISSLVDVGGGIGAAAQAISKAFPHVKCSVLDLAHVVAKAPTHTDVQFIAGDMFESI 256

HSP 2 Score: 35.8094 bits (81), Expect = 3.571e-16
Identity = 13/30 (43.33%), Postives = 23/30 (76.67%), Query Frame = 3 
Query:  783 PEGDAILMKGMLHNFDDENCIKILRIVTKS 872
            P  DA+L+K +LH++D ++C+KIL+   K+
Sbjct:  257 PPADAVLLKSVLHDWDHDDCVKILKNCKKA 286
[Close]
BLAST of EY692471 vs. ExPASy Swiss-Prot
Match: EOMT1_OCIBA (Eugenol O-methyltransferase OS=Ocimum basilicum GN=EOMT1 PE=1 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.918e-13
Identity = 54/250 (21.60%), Postives = 105/250 (42.00%), Query Frame = 1 
Query:   46 SKSKVTNEEEEVYSHAMVLGSAIVLPAVFQAVVNLDVLDIINKAGPGAELSASEIASQIPTKNPEGAAKMLDRVLGLLVSFNILCCSFSTHGVRL-YSLAPVAKYYVSDQNGVSMRPYMNLSLDKVMMPGWFQLKDQILEGE--LSYNKALGMDFYDYLGTDSRFNEVFNNGMIGQTSFVINKIVESYKG-FQNITRLVDVGGGLXITLATIISKYPHIKGINFDVPHVIKDASSYPGMEHXGRDMYKKI 783
            S  ++   +  V++H     +++ L    Q    L + DI++K G    ++ S++   IP    +   +   R++  LV+ N      +++   + Y L P A   +  +  +++ P + + LD      W  + +     +    +  A G  F++ L  +      F+  M   +  + +   + YK   + I  LVDVGGG       I+   P IK    D+PHV+    S   + + G DM++ I
Sbjct:   11 STEQLLQAQVHVWNHMYAFANSMSLKCAIQ----LGIPDILHKHG--RPMTLSQLLQSIPINKEK--TQCFQRLMRALVNSNFFIEENNSNNQEVCYWLTP-ASCLLLKEAPLTVTPLVQVVLDPTFTNPWHHMSEWFTHEKHATQFEAANGCTFWEKLANEPSKGRFFDEAMSCDSRLIAHVFTKDYKHVIEGIRTLVDVGGGNGTMAKAIVEAMPTIKCTVIDLPHVVAGLESTDNLNYIGGDMFQSI 251

HSP 2 Score: 31.9574 bits (71), Expect = 1.918e-13
Identity = 13/28 (46.43%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  774 QKNPEGDAILMKGMLHNFDDENCIKILR 857
            Q  P  DAIL+K ++H++DD   +KIL+
Sbjct:  249 QSIPSADAILLKSIIHDWDDVEGLKILK 276
[Close]
BLAST of EY692471 vs. ExPASy Swiss-Prot
Match: OMT11_DICDI (Probable inactive O-methyltransferase 11 OS=Dictyostelium discoideum GN=omt11 PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 2.496e-13
Identity = 32/120 (26.67%), Postives = 65/120 (54.17%), Query Frame = 1 
Query:  439 QLKDQILE-GELSYNKALGM-DFYDYLGTDSRFNEVFNNGMIGQTSFVINKIVESYKGFQNITRLVDVGGGLXITLATIISKYPHIKGINFDVPHVIKDAS---SYPGMEHXGRDMYKKI 783
            Q+  + LE GE     ++G+ D+++ +  +  +   FN+GMIG T+ ++ K ++          +VD+GG     + +++ +YP++ GINFD   VI  ++    +P ++H   D +K +
Sbjct:  112 QVLPETLEIGENQGPSSIGLSDYWEQIEKNEIYKNEFNDGMIGYTTHIL-KFLKGKIDLSKFETVVDIGGSHGYLIGSLLDRYPNVNGINFDTDMVINSSNEKYQHPRLKHVAGDFFKSV 230

HSP 2 Score: 35.4242 bits (80), Expect = 2.496e-13
Identity = 15/30 (50.00%), Postives = 21/30 (70.00%), Query Frame = 3 
Query:  783 PEGDAILMKGMLHNFDDENCIKILRIVTKS 872
            PE D  LMK +L  F DE C ++L+I++KS
Sbjct:  231 PEADCYLMKLILRCFSDEKCCELLKIISKS 260
[Close]
BLAST of EY692471 vs. ExPASy Swiss-Prot
Match: OMT12_DICDI (O-methyltransferase 12 OS=Dictyostelium discoideum GN=omt12 PE=1 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 3.232e-13
Identity = 26/101 (25.74%), Postives = 54/101 (53.47%), Query Frame = 1 
Query:  499 FYDYLGTDSRFNEVFNNGMIGQTSFVINKIVESYKGFQNITRLVDVGGGLXITLATIISKYPHIKGINFDVPHVIKDASS------YPGMEHXGRDMYKKI 783
            F+++  TD  + ++F+N M   TS +I++++       N   +VD+GG     +  ++   P+I GINFD+ ++I  ++S      +P ++H   D +  +
Sbjct:  135 FWEHFETDESYKQLFHNAMKDYTSLIIDRLISKISLSPNFKTVVDIGGSHGFLIGKLLESNPNIHGINFDLENIINSSTSKNENFQHPRLKHVSGDFFNSV 235

HSP 2 Score: 33.8834 bits (76), Expect = 3.232e-13
Identity = 14/30 (46.67%), Postives = 20/30 (66.67%), Query Frame = 3 
Query:  783 PEGDAILMKGMLHNFDDENCIKILRIVTKS 872
            PE D  ++K +LH++ DE CI IL  + KS
Sbjct:  236 PEADCYILKYILHDWSDEKCITILNNIHKS 265
[Close]
BLAST of EY692471 vs. ExPASy Swiss-Prot
Match: CVMT1_OCIBA (Chavicol O-methyltransferase OS=Ocimum basilicum GN=CVOMT1 PE=1 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 3.235e-13
Identity = 53/249 (21.29%), Postives = 101/249 (40.56%), Query Frame = 1 
Query:   46 SKSKVTNEEEEVYSHAMVLGSAIVLPAVFQAVVNLDVLDIINKAGPGAELSASEIASQIPTKNPEGAAKMLDRVLGLLVSFNILCCSFSTHGVRLYSLAPVAKYYVSDQNGVSMRPYMNLSLDKVMMPGWFQLKDQILEGE--LSYNKALGMDFYDYLGTDSRFNEVFNNGMIGQTSFVINKIVESYKG-FQNITRLVDVGGGLXITLATIISKYPHIKGINFDVPHVIKDASSYPGMEHXGRDMYKKI 783
            S  ++   +  V++H     +++ L    Q    L + DI++K      ++ S++   IP    +  ++   R++  LV+ N      S +    Y L P ++  +     +++ P + + LD      W  + +          +  A G  F++ L         F+  M   +  V + + + YK     I  LVDVGGG       I+   P +K    D+PHV+    S   + + G DM++ I
Sbjct:   11 STEQLLQAQAHVWNHMYAFANSMSLKCAIQ----LGIPDILHKHDH--PMTLSQLLKAIPINKEK--SQSFQRLMRALVNSNFFIEENSNNQEVCYWLTPASRLLLKGAP-LTVAPLVQVVLDPTFTNPWHYMSEWFKHENHATQFEAANGCTFWEKLANKPSMGRFFDEAMSCDSRLVAHVLTKDYKHVIDGIRTLVDVGGGNGTMAKAIVEAVPTMKCTVLDLPHVVAGLESTDKLSYIGGDMFQSI 250

HSP 2 Score: 33.4982 bits (75), Expect = 3.235e-13
Identity = 14/28 (50.00%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  774 QKNPEGDAILMKGMLHNFDDENCIKILR 857
            Q  P  DAIL+K ++H++DDE  +KIL+
Sbjct:  248 QSIPSADAILLKFIIHDWDDEEGLKILK 275
[Close]
BLAST of EY692471 vs. ExPASy Swiss-Prot
Match: OMT4_DICDI (O-methyltransferase 4 OS=Dictyostelium discoideum GN=omt4 PE=3 SV=1)

HSP 1 Score: 46.595 bits (109), Expect = 7.808e-11
Identity = 31/113 (27.43%), Postives = 52/113 (46.02%), Query Frame = 1 
Query:  463 GELSYNKALGMDFYDYLGTDSRFNEVFNNGMIGQTSFVINKIVESYKGFQNITRLVDVGGGLXITLATIISKYPHIKGINFDVPHVIKDASSY---PGMEHXGRDMYKKIQRG 792
            GE   +K+LG++ +  L       E F N M   +S  I   ++ +  F    ++VD+GG     +  I+ KYP+  GINFD+      A      P +EH   + ++ +  G
Sbjct:  127 GEPQCHKSLGVNSWWDLIKKPGEEEFFKNAMKVSSSEAIESALK-FIDFSPFKKIVDIGGSHGRFVCEILEKYPNSHGINFDLESFFNGAGELIKNPRLEHKSGNFFESVPEG 238

HSP 2 Score: 40.0466 bits (92), Expect = 7.808e-11
Identity = 16/35 (45.71%), Postives = 25/35 (71.43%), Query Frame = 3 
Query:  768 YVQKNPEGDAILMKGMLHNFDDENCIKILRIVTKS 872
            + +  PEGD  ++K +LH++ DE+CIKIL  + KS
Sbjct:  231 FFESVPEGDCYILKRILHDWKDEDCIKILETIGKS 265
The following BLAST results are available for this feature:
BLAST of EY692471 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 39
Match NameE-valueIdentityDescription
7OMT9_MEDSA4.130e-1824.19Isoflavone-7-O-methyltransferase 9 OS=Medicago sat... [more]
7OMT8_MEDSA5.370e-1824.19Isoflavone-7-O-methyltransferase 8 OS=Medicago sat... [more]
7OMT6_MEDSA5.370e-1824.19Isoflavone-7-O-methyltransferase 6 OS=Medicago sat... [more]
ZRP4_MAIZE3.571e-1623.11O-methyltransferase ZRP4 OS=Zea mays GN=ZRP4 PE=2 ... [more]
EOMT1_OCIBA1.918e-1321.60Eugenol O-methyltransferase OS=Ocimum basilicum GN... [more]
OMT11_DICDI2.496e-1326.67Probable inactive O-methyltransferase 11 OS=Dictyo... [more]
OMT12_DICDI3.232e-1325.74O-methyltransferase 12 OS=Dictyostelium discoideum... [more]
CVMT1_OCIBA3.235e-1321.29Chavicol O-methyltransferase OS=Ocimum basilicum G... [more]
OMT4_DICDI7.808e-1127.43O-methyltransferase 4 OS=Dictyostelium discoideum ... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-700-001-A05-CT.F Sweet orange fruit, development stadium (1 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY692471 ID=EY692471; Name=EY692471; organism=Citrus sinensis; type=EST; length=892bp
CCACGCGTCCGGAAATTAAACGCGCAAATGGGTTCTTTCACTAAGAGCAA
ATCAAAAGTGACCAATGAAGAGGAAGAGGTATATTCACATGCCATGGTAC
TGGGAAGTGCAATAGTGCTACCCGCGGTTTTTCAAGCAGTAGTAAATCTT
GATGTTCTTGATATCATAAACAAAGCTGGTCCTGGTGCTGAGCTTTCTGC
TTCAGAGATTGCTTCTCAAATTCCCACCAAAAATCCTGAGGGTGCTGCCA
AGATGTTGGATCGGGTTCTCGGGCTTCTGGTGAGCTTCAATATCCTTTGT
TGCTCTTTCAGTACTCATGGAGTAAGGCTCTACAGTCTCGCTCCTGTCGC
TAAATACTATGTGAGTGATCAAAACGGCGTCTCAATGCGTCCCTATATGA
ACTTGTCTCTTGACAAAGTAATGATGCCTGGCTGGTTCCAATTGAAAGAC
CAAATTCTTGAAGGAGAACTTTCGTATAATAAAGCCCTCGGAATGGATTT
CTACGATTATCTGGGCACCGACTCCAGGTTCAATGAAGTTTTCAACAATG
GAATGATCGGCCAGACTTCTTTTGTAATCAATAAGATTGTAGAATCCTAC
AAAGGCTTTCAGAATATTACGCGCCTTGTTGACGTTGGCGGTGGTTTANG
AATCACCCTTGCGACCATAATTTCCAAATACCCTCATATTAAAGGTATTA
ATTTTGACGTACCTCATGTTATAAAAGATGCCTCATCTTATCCCGGCATG
GAACATGNTGGGCGAGATATGTACAAAAAAATCCAGAGGGGGATGCCATT
TTAATGAAGGGGATGCTTCATAATTTTGATGATGAGAATTGCATAAAGAT
ACTGAGGATTGTTACAAAGTCTTCCAATGATGGAAAGTACTT
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