EY711167 | Citrus Genome Database

Overview


Name	EY711167
Unique Name	EY711167
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	849

Libraries


Library Name	Type
Sweet orange fruit, development stadium (3 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY711167 vs. ExPASy Swiss-Prot
Match: NEDD8_CAEEL (NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.368e-19
Identity = 46/76 (60.53%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  139 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 366
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76

HSP 2 Score: 79.7221 bits (195), Expect = 1.796e-15
Identity = 36/59 (61.02%), Postives = 47/59 (79.66%), Query Frame = 1

Query:  367 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADY 543
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADY 59

HSP 3 Score: 21.9422 bits (45), Expect = 1.796e-15
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 2

Query:  557 ATLHLVLRLRGG 592
            + LHLVL LRGG
Sbjct:   65 SVLHLVLALRGG 76

[Close]

BLAST of EY711167 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 96.6709 bits (239), Expect = 1.787e-19
Identity = 46/75 (61.33%), Postives = 59/75 (78.67%), Query Frame = 1

Query:  145 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 369
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

HSP 2 Score: 77.411 bits (189), Expect = 5.130e-15
Identity = 35/57 (61.40%), Postives = 46/57 (80.70%), Query Frame = 1

Query:  373 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADY 543
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDY 59

HSP 3 Score: 22.7126 bits (47), Expect = 5.130e-15
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = 2

Query:  557 ATLHLVLRLRGGM 595
            + LHLVL LRGG+
Sbjct:   65 SVLHLVLALRGGL 77

[Close]

BLAST of EY711167 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.975e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  139 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 366
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 75.485 bits (184), Expect = 3.217e-14
Identity = 32/59 (54.24%), Postives = 47/59 (79.66%), Query Frame = 1

Query:  367 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADY 543
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADY 59

HSP 3 Score: 21.9422 bits (45), Expect = 3.217e-14
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 2

Query:  557 ATLHLVLRLRGG 592
            + LHLVL LRGG
Sbjct:   65 SVLHLVLALRGG 76

[Close]

BLAST of EY711167 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.975e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  139 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 366
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 75.485 bits (184), Expect = 3.217e-14
Identity = 32/59 (54.24%), Postives = 47/59 (79.66%), Query Frame = 1

Query:  367 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADY 543
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADY 59

HSP 3 Score: 21.9422 bits (45), Expect = 3.217e-14
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 2

Query:  557 ATLHLVLRLRGG 592
            + LHLVL LRGG
Sbjct:   65 SVLHLVLALRGG 76

[Close]

BLAST of EY711167 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 1.975e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  139 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 366
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 75.485 bits (184), Expect = 3.217e-14
Identity = 32/59 (54.24%), Postives = 47/59 (79.66%), Query Frame = 1

Query:  367 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADY 543
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADY 59

HSP 3 Score: 21.9422 bits (45), Expect = 3.217e-14
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 2

Query:  557 ATLHLVLRLRGG 592
            + LHLVL LRGG
Sbjct:   65 SVLHLVLALRGG 76

[Close]

BLAST of EY711167 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.975e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  139 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 366
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 75.485 bits (184), Expect = 3.217e-14
Identity = 32/59 (54.24%), Postives = 47/59 (79.66%), Query Frame = 1

Query:  367 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADY 543
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADY 59

HSP 3 Score: 21.9422 bits (45), Expect = 3.217e-14
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 2

Query:  557 ATLHLVLRLRGG 592
            + LHLVL LRGG
Sbjct:   65 SVLHLVLALRGG 76

[Close]

BLAST of EY711167 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.975e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  139 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 366
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 75.485 bits (184), Expect = 3.217e-14
Identity = 32/59 (54.24%), Postives = 47/59 (79.66%), Query Frame = 1

Query:  367 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADY 543
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADY 59

HSP 3 Score: 21.9422 bits (45), Expect = 3.217e-14
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 2

Query:  557 ATLHLVLRLRGG 592
            + LHLVL LRGG
Sbjct:   65 SVLHLVLALRGG 76

[Close]

BLAST of EY711167 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.580e-18
Identity = 41/76 (53.95%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  139 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 366
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

HSP 2 Score: 73.1738 bits (178), Expect = 1.550e-13
Identity = 30/59 (50.85%), Postives = 46/59 (77.97%), Query Frame = 1

Query:  367 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADY 543
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDY 59

HSP 3 Score: 21.9422 bits (45), Expect = 1.550e-13
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 2

Query:  557 ATLHLVLRLRGG 592
            + LHLVL LRGG
Sbjct:   65 SVLHLVLALRGG 76

[Close]

BLAST of EY711167 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.672e-17
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = 1

Query:  139 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 366
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

HSP 2 Score: 68.9366 bits (167), Expect = 2.764e-12
Identity = 31/65 (47.69%), Postives = 45/65 (69.23%), Query Frame = 1

Query:  367 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYTFRKSN 561
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   Y   + +
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGS 65

HSP 3 Score: 21.9422 bits (45), Expect = 2.764e-12
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 2

Query:  557 ATLHLVLRLRGG 592
            + LHLVL LRGG
Sbjct:   65 SVLHLVLALRGG 76

[Close]

BLAST of EY711167 vs. ExPASy Swiss-Prot
Match: NEDD8_DICDI (NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 2.184e-17
Identity = 41/77 (53.25%), Postives = 58/77 (75.32%), Query Frame = 1

Query:  139 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 369
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77

HSP 2 Score: 69.707 bits (169), Expect = 9.700e-13
Identity = 30/60 (50.00%), Postives = 45/60 (75.00%), Query Frame = 1

Query:  367 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYT 546
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYS 60

HSP 3 Score: 22.7126 bits (47), Expect = 9.700e-13
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = 2

Query:  557 ATLHLVLRLRGGM 595
            + LHLVL LRGG+
Sbjct:   65 SVLHLVLALRGGL 77

The following BLAST results are available for this feature:

BLAST of EY711167 vs. ExPASy Swiss-Prot

BLAST of EY711167 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 95


Match Name	E-value	Identity	Description
NEDD8_CAEEL	1.368e-19	60.53	NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1	[more]
RUB2_ARATH	1.787e-19	61.33	NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN...	[more]
NEDD8_RAT	1.975e-18	56.58	NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1	[more]
NEDD8_RABIT	1.975e-18	56.58	NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1	[more]
NEDD8_MOUSE	1.975e-18	56.58	NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2	[more]
NEDD8_HUMAN	1.975e-18	56.58	NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1	[more]
NEDD8_BOVIN	1.975e-18	56.58	NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1	[more]
RUB3_ORYSJ	2.580e-18	53.95	NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap...	[more]
RUB3_ARATH	1.672e-17	55.26	NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN...	[more]
NEDD8_DICDI	2.184e-17	53.25	NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-702-004-F03-CT.F Sweet orange fruit, development stadium (3 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY711167 ID=EY711167; Name=EY711167; organism=Citrus sinensis; type=EST; length=849bp
CAAGACAAGGAGGGAATCCCCCCGGACCAGCAGAGGCTTATCTTCGCTGG
AAAGCAGCTCGAGGACGGCCGCACCTTGGCCGATTATAACATACAGAAGG
AGTCAACCCTCCATCTTGTCCTTCGTCTCCGCGGAGGCATGCAGATCTTT
GTCAAAACCCTAACCGGGAAGACGATAACCCTCGAGGTTGAGAGCTCAGA
CACGATCGATAACGTCAAGGCCAAGATTCAAGACAAGGAGGGAATCCCCC
CGGACCAGCAGAGGCTGATCTTTGCTGGCAAGCAACTGGAAGACGGCCGC
ACCTTGGCTGATTACAATATCCAGAAGGAGTCAACCCTTCATCTCGTCCT
CAGGCTCCGTGGTGGTATGCAGATCTTCGTCAAAACCCTAACTGGGAAGA
CGATCACTCTGGAGGTGGAGAGTTCGGATACGATTGATAACGTCAAGGCC
AAGATCCAGGACAAAGAGGGGATCCCGCCGGACCAGCAAAGATTGATCTT
CGCCGGTAAGCAGTTGGAAGATGGCCGCACCTTGGCCGATTACACATTCA
GAAAGAGCAACTCTCCACCTTGTTCTTCGTCTCAGGGGAGGCATGCAGAT
ATTTGTTAANGACCCTTACTGGGAAAACAATTTACTTTGAAAGTGAGAGC
TCAGACACGATTTTGACATGTCAGGGCAAAATCCNNAGATAGGAAAGGAT
TCCACNGNATCAGCAGCGTTTGATCTTCGCTGGCAACAGCTTGAGGATGG
AAAGACTCTTGCGGGATAATACATCCAGAAGGAGTCGACCCTTCATCTGG
NTCTGCGCCTGAGGGTGGTATGCAAATATTTGTCAGACCTGACTGGAAG