EY711062 | Citrus Genome Database

Overview


Name	EY711062
Unique Name	EY711062
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	891

Libraries


Library Name	Type
Sweet orange fruit, development stadium (3 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY711062 vs. ExPASy Swiss-Prot
Match: CRY1_SYLBO (Cryptochrome-1 OS=Sylvia borin GN=CRY1 PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 5.847e-16
Identity = 47/156 (30.13%), Postives = 72/156 (46.15%), Query Frame = 2

Query:   41 LSPHLHFGEVSVRKVFHLVRIKQVSWANEGNKAGDESVNLFLKSIGLREYSRYMSFNHPYSHERPLLGHLKFFSWVVDEGYFKAWRQGRTGYPLVDAGMRELWATGWLHDRIRVVVSSFFVK-VLQLPWRWGMKYFWDTLLDADLESDALGWQYIS 505
            LSP+L FG +S R    L   K      +  K     ++L+ + +  RE+    + N+P   +           W  +      W +GRTG+P +DA M +L   GW+H   R  V+ F  +  L + W  GMK F + LLDAD   +A  W ++S
Sbjct:  251 LSPYLRFGCLSCR----LFYFKLTDLYKKVKKNSSPPLSLYGQLLW-REFFYTAATNNPRFDKMEGNPICVQIPWDKNPEALAKWAEGRTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWISWEEGMKVFEELLLDADWSVNAGSWMWLS 401

HSP 2 Score: 35.039 bits (79), Expect = 5.847e-16
Identity = 15/32 (46.88%), Postives = 21/32 (65.62%), Query Frame = 1

Query:  559 GYKFDPNGEYVRRWLPELARLPTEWIPTHGNA 654
            G + DPNG+Y+RR+LP L   P ++I    NA
Sbjct:  419 GRRTDPNGDYIRRYLPVLRGFPAKYIYDPWNA 450

HSP 3 Score: 25.409 bits (54), Expect = 5.847e-16
Identity = 10/24 (41.67%), Postives = 15/24 (62.50%), Query Frame = 3

Query:  642 PWKCPKSGLQAAGMELGSHYPFPL 713
            PW  P+S  +AA   +G +YP P+
Sbjct:  447 PWNAPESIQKAAKCIIGVNYPKPM 470

[Close]

BLAST of EY711062 vs. ExPASy Swiss-Prot
Match: CRY1_ERIRU (Cryptochrome-1 OS=Erithacus rubecula GN=CRY1 PE=2 SV=2)

HSP 1 Score: 64.6994 bits (156), Expect = 5.847e-16
Identity = 47/156 (30.13%), Postives = 72/156 (46.15%), Query Frame = 2

Query:   41 LSPHLHFGEVSVRKVFHLVRIKQVSWANEGNKAGDESVNLFLKSIGLREYSRYMSFNHPYSHERPLLGHLKFFSWVVDEGYFKAWRQGRTGYPLVDAGMRELWATGWLHDRIRVVVSSFFVK-VLQLPWRWGMKYFWDTLLDADLESDALGWQYIS 505
            LSP+L FG +S R    L   K      +  K     ++L+ + +  RE+    + N+P   +           W  +      W +GRTG+P +DA M +L   GW+H   R  V+ F  +  L + W  GMK F + LLDAD   +A  W ++S
Sbjct:  251 LSPYLRFGCLSCR----LFYFKLTDLYKKVKKNSSPPLSLYGQLLW-REFFYTAATNNPRFDKMEGNPICVQIPWDKNPEALAKWAEGRTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWISWEEGMKVFEELLLDADWSVNAGSWMWLS 401

HSP 2 Score: 35.039 bits (79), Expect = 5.847e-16
Identity = 15/32 (46.88%), Postives = 21/32 (65.62%), Query Frame = 1

Query:  559 GYKFDPNGEYVRRWLPELARLPTEWIPTHGNA 654
            G + DPNG+Y+RR+LP L   P ++I    NA
Sbjct:  419 GRRTDPNGDYIRRYLPVLRGFPAKYIYDPWNA 450

HSP 3 Score: 25.409 bits (54), Expect = 5.847e-16
Identity = 10/24 (41.67%), Postives = 15/24 (62.50%), Query Frame = 3

Query:  642 PWKCPKSGLQAAGMELGSHYPFPL 713
            PW  P+S  +AA   +G +YP P+
Sbjct:  447 PWNAPESIQKAAKCIIGVNYPKPM 470

[Close]

BLAST of EY711062 vs. ExPASy Swiss-Prot
Match: CRYD_OSTTA (Cryptochrome DASH, chloroplastic/mitochondrial OS=Ostreococcus tauri GN=Ot01g06320 PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 7.731e-16
Identity = 52/161 (32.30%), Postives = 77/161 (47.83%), Query Frame = 2

Query:   41 LSPHLHFGEVSVRKVFHLVRIKQVSWANEGNKAGDESVNLFLKSIGLREYSRYMSFNHPYSHERPLLGHL-----KFFSWVVDEGYFKAWRQGRTGYPLVDAGMRELWATGWLHDRIRVVVSSFFVKVLQLPWRWGMKYFWDTLLDADLESDALGWQYISG 508
            L+P L  G VS R V   +R        E  +  ++S    +  +  R++ ++ +  H        + HL     +  SW  DE   KAW+ G TGYPL+DA MREL ATG++ +R R  V+S+      + WR G  +F   LLD D  S+   W   +G
Sbjct:  283 LAPWLALGCVSPRHVVSEIR------RYESERVENKSTYWVIFELIWRDFFKFFALKHGNK-----IFHLDGTAGRRASWKRDEKILKAWKTGTTGYPLIDANMRELAATGFMSNRGRQNVASWLALDAGIDWRHGADWFEHHLLDYDTASNWGNWCAAAG 432

HSP 2 Score: 32.7278 bits (73), Expect = 7.731e-16
Identity = 11/23 (47.83%), Postives = 17/23 (73.91%), Query Frame = 1

Query:  568 FDPNGEYVRRWLPELARLPTEWI 636
            +DP GEY++ W+ ELA +P  +I
Sbjct:  450 YDPAGEYIKTWVKELAEVPAAYI 472

[Close]

BLAST of EY711062 vs. ExPASy Swiss-Prot
Match: CRY1_MOUSE (Cryptochrome-1 OS=Mus musculus GN=Cry1 PE=1 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 4.603e-15
Identity = 47/156 (30.13%), Postives = 72/156 (46.15%), Query Frame = 2

Query:   41 LSPHLHFGEVSVRKVFHLVRIKQVSWANEGNKAGDESVNLFLKSIGLREYSRYMSFNHPYSHERPLLGHLKFFSWVVDEGYFKAWRQGRTGYPLVDAGMRELWATGWLHDRIRVVVSSFFVK-VLQLPWRWGMKYFWDTLLDADLESDALGWQYIS 505
            LSP+L FG +S R    L   K      +  K     ++L+ + +  RE+    + N+P   +           W  +      W +GRTG+P +DA M +L   GW+H   R  V+ F  +  L + W  GMK F + LLDAD   +A  W ++S
Sbjct:  251 LSPYLRFGCLSCR----LFYFKLTDLYKKVKKNSSPPLSLYGQLLW-REFFYTAATNNPRFDKMEGNPICVQIPWDKNPEALAKWAEGRTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWISWEEGMKVFEELLLDADWSINAGSWMWLS 401

HSP 2 Score: 35.039 bits (79), Expect = 4.603e-15
Identity = 15/32 (46.88%), Postives = 21/32 (65.62%), Query Frame = 1

Query:  559 GYKFDPNGEYVRRWLPELARLPTEWIPTHGNA 654
            G + DPNG+Y+RR+LP L   P ++I    NA
Sbjct:  419 GRRTDPNGDYIRRYLPVLRGFPAKYIYDPWNA 450

HSP 3 Score: 22.3274 bits (46), Expect = 4.603e-15
Identity = 8/24 (33.33%), Postives = 13/24 (54.17%), Query Frame = 3

Query:  642 PWKCPKSGLQAAGMELGSHYPFPL 713
            PW  P+   + A   +G +YP P+
Sbjct:  447 PWNAPEGIQKVAKCLIGVNYPKPM 470

[Close]

BLAST of EY711062 vs. ExPASy Swiss-Prot
Match: CRY1_RAT (Cryptochrome-1 OS=Rattus norvegicus GN=Cry1 PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 4.609e-15
Identity = 47/156 (30.13%), Postives = 72/156 (46.15%), Query Frame = 2

Query:   41 LSPHLHFGEVSVRKVFHLVRIKQVSWANEGNKAGDESVNLFLKSIGLREYSRYMSFNHPYSHERPLLGHLKFFSWVVDEGYFKAWRQGRTGYPLVDAGMRELWATGWLHDRIRVVVSSFFVK-VLQLPWRWGMKYFWDTLLDADLESDALGWQYIS 505
            LSP+L FG +S R    L   K      +  K     ++L+ + +  RE+    + N+P   +           W  +      W +GRTG+P +DA M +L   GW+H   R  V+ F  +  L + W  GMK F + LLDAD   +A  W ++S
Sbjct:  251 LSPYLRFGCLSCR----LFYFKLTDLYKKVKKNSSPPLSLYGQLLW-REFFYTAATNNPRFDKMEGNPICVQIPWDKNPEALAKWAEGRTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWISWEEGMKVFEELLLDADWSINAGSWMWLS 401

HSP 2 Score: 35.039 bits (79), Expect = 4.609e-15
Identity = 15/32 (46.88%), Postives = 21/32 (65.62%), Query Frame = 1

Query:  559 GYKFDPNGEYVRRWLPELARLPTEWIPTHGNA 654
            G + DPNG+Y+RR+LP L   P ++I    NA
Sbjct:  419 GRRTDPNGDYIRRYLPVLRGFPAKYIYDPWNA 450

HSP 3 Score: 22.3274 bits (46), Expect = 4.609e-15
Identity = 8/24 (33.33%), Postives = 13/24 (54.17%), Query Frame = 3

Query:  642 PWKCPKSGLQAAGMELGSHYPFPL 713
            PW  P+   + A   +G +YP P+
Sbjct:  447 PWNAPEGIQKVAKCLIGVNYPKPM 470

[Close]

BLAST of EY711062 vs. ExPASy Swiss-Prot
Match: CRY1_HUMAN (Cryptochrome-1 OS=Homo sapiens GN=CRY1 PE=1 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 4.610e-15
Identity = 47/156 (30.13%), Postives = 72/156 (46.15%), Query Frame = 2

Query:   41 LSPHLHFGEVSVRKVFHLVRIKQVSWANEGNKAGDESVNLFLKSIGLREYSRYMSFNHPYSHERPLLGHLKFFSWVVDEGYFKAWRQGRTGYPLVDAGMRELWATGWLHDRIRVVVSSFFVK-VLQLPWRWGMKYFWDTLLDADLESDALGWQYIS 505
            LSP+L FG +S R    L   K      +  K     ++L+ + +  RE+    + N+P   +           W  +      W +GRTG+P +DA M +L   GW+H   R  V+ F  +  L + W  GMK F + LLDAD   +A  W ++S
Sbjct:  251 LSPYLRFGCLSCR----LFYFKLTDLYKKVKKNSSPPLSLYGQLLW-REFFYTAATNNPRFDKMEGNPICVQIPWDKNPEALAKWAEGRTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWISWEEGMKVFEELLLDADWSINAGSWMWLS 401

HSP 2 Score: 35.039 bits (79), Expect = 4.610e-15
Identity = 15/32 (46.88%), Postives = 21/32 (65.62%), Query Frame = 1

Query:  559 GYKFDPNGEYVRRWLPELARLPTEWIPTHGNA 654
            G + DPNG+Y+RR+LP L   P ++I    NA
Sbjct:  419 GRRTDPNGDYIRRYLPVLRGFPAKYIYDPWNA 450

HSP 3 Score: 22.3274 bits (46), Expect = 4.610e-15
Identity = 8/24 (33.33%), Postives = 13/24 (54.17%), Query Frame = 3

Query:  642 PWKCPKSGLQAAGMELGSHYPFPL 713
            PW  P+   + A   +G +YP P+
Sbjct:  447 PWNAPEGIQKVAKCLIGVNYPKPM 470

[Close]

BLAST of EY711062 vs. ExPASy Swiss-Prot
Match: CRY1_SPAJD (Cryptochrome-1 OS=Spalax judaei GN=CRY1 PE=2 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 7.714e-15
Identity = 47/156 (30.13%), Postives = 71/156 (45.51%), Query Frame = 2

Query:   41 LSPHLHFGEVSVRKVFHLVRIKQVSWANEGNKAGDESVNLFLKSIGLREYSRYMSFNHPYSHERPLLGHLKFFSWVVDEGYFKAWRQGRTGYPLVDAGMRELWATGWLHDRIRVVVSSFFV-KVLQLPWRWGMKYFWDTLLDADLESDALGWQYIS 505
            LSP+L FG +S R    L   K      +  K     ++L+ + +  RE+    + N+P   +           W  +      W +GRTG+P +DA M +L   GW+H   R  V+ F     L + W  GMK F + LLDAD   +A  W ++S
Sbjct:  251 LSPYLRFGCLSCR----LFYFKLTDLYKKVKKNSSPPLSLYGQLLW-REFFYTAATNNPRFDKMEGNPICVQIPWDKNPEALAKWAEGRTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTGSDLWISWEEGMKVFEELLLDADWSINAGSWMWLS 401

HSP 2 Score: 35.039 bits (79), Expect = 7.714e-15
Identity = 15/32 (46.88%), Postives = 21/32 (65.62%), Query Frame = 1

Query:  559 GYKFDPNGEYVRRWLPELARLPTEWIPTHGNA 654
            G + DPNG+Y+RR+LP L   P ++I    NA
Sbjct:  419 GRRTDPNGDYIRRYLPVLRGFPAKYIYDPWNA 450

HSP 3 Score: 22.3274 bits (46), Expect = 7.714e-15
Identity = 8/24 (33.33%), Postives = 13/24 (54.17%), Query Frame = 3

Query:  642 PWKCPKSGLQAAGMELGSHYPFPL 713
            PW  P+   + A   +G +YP P+
Sbjct:  447 PWNAPEGIQKVAKCLIGVNYPKPM 470

[Close]

BLAST of EY711062 vs. ExPASy Swiss-Prot
Match: CRYD_GLOVI (Cryptochrome DASH OS=Gloeobacter violaceus GN=cry PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 8.186e-15
Identity = 50/138 (36.23%), Postives = 69/138 (50.00%), Query Frame = 2

Query:  263 WVVDEGYFKAWRQGRTGYPLVDAGMRELWATGWLHDRIRVVVSSFFVKVLQLPWRWGMKYFWDTLLDADLESDALGWQYISGTIPDGRAFDRIDIHSLRVTSLTPMENMYDGGSLN----LPDCPLNGYPPMEMPKVW 664
            W  D   F+ WR G+TG+PLVDA MREL ATG++ +R R  V+SF  K L + W  G ++F   L+D D+ S+   W Y +G   D R F        R  ++      YD         LP+  L G PP  + + W
Sbjct:  318 WKEDWERFELWRTGQTGFPLVDANMRELAATGFMSNRGRQNVASFLTKNLGIHWHMGAEWFESRLIDYDVASNWGNWNYTAGVGNDARGF--------RFFNILKQARDYDPDGAYVKHWLPE--LAGLPPARVHEPW 445

HSP 2 Score: 21.1718 bits (43), Expect = 8.186e-15
Identity = 9/24 (37.50%), Postives = 12/24 (50.00%), Query Frame = 3

Query:  642 PWKCPKSGLQAAGMELGSHYPFPL 713
            PWK      +  G+ LG  YP P+
Sbjct:  444 PWKLLPVEQKRFGVRLGVDYPQPV 467

[Close]

BLAST of EY711062 vs. ExPASy Swiss-Prot
Match: CRY1_MACFA (Cryptochrome-1 OS=Macaca fascicularis GN=CRY1 PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 9.982e-15
Identity = 46/156 (29.49%), Postives = 72/156 (46.15%), Query Frame = 2

Query:   41 LSPHLHFGEVSVRKVFHLVRIKQVSWANEGNKAGDESVNLFLKSIGLREYSRYMSFNHPYSHERPLLGHLKFFSWVVDEGYFKAWRQGRTGYPLVDAGMRELWATGWLHDRIRVVVSSFFVK-VLQLPWRWGMKYFWDTLLDADLESDALGWQYIS 505
            LSP+L FG +S R    L   K      +  +     ++L+ + +  RE+    + N+P   +           W  +      W +GRTG+P +DA M +L   GW+H   R  V+ F  +  L + W  GMK F + LLDAD   +A  W ++S
Sbjct:  251 LSPYLRFGCLSCR----LFYFKLTDLYKKVKRNSSPPLSLYGQLLW-REFFYTAATNNPRFDKMEGNPICVQIPWDKNPEALAKWAEGRTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWISWEEGMKVFEELLLDADWSINAGSWMWLS 401

HSP 2 Score: 35.039 bits (79), Expect = 9.982e-15
Identity = 15/32 (46.88%), Postives = 21/32 (65.62%), Query Frame = 1

Query:  559 GYKFDPNGEYVRRWLPELARLPTEWIPTHGNA 654
            G + DPNG+Y+RR+LP L   P ++I    NA
Sbjct:  419 GRRTDPNGDYIRRYLPVLRGFPAKYIYDPWNA 450

HSP 3 Score: 22.3274 bits (46), Expect = 9.982e-15
Identity = 8/24 (33.33%), Postives = 13/24 (54.17%), Query Frame = 3

Query:  642 PWKCPKSGLQAAGMELGSHYPFPL 713
            PW  P+   + A   +G +YP P+
Sbjct:  447 PWNAPEGIQKVAKCLIGINYPKPM 470

[Close]

BLAST of EY711062 vs. ExPASy Swiss-Prot
Match: CRYD_IDILO (Cryptochrome DASH OS=Idiomarina loihiensis GN=cry PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.067e-14
Identity = 36/88 (40.91%), Postives = 55/88 (62.50%), Query Frame = 2

Query:  284 FKAWRQGRTGYPLVDAGMRELWATGWLHDRIRVVVSSFFVKVLQLPWRWGMKYFWDTLLDADLESDALGWQYISGTIPDGRAFDRIDI 547
            F+ W  G T YP+V+A M++L ATG++ +R R +V+S FV  L L WR+G  YF   L+D D+ S+   WQY++G   D R   + ++
Sbjct:  325 FQKWCSGNTPYPIVNACMKQLNATGYMSNRGRQLVASCFVHELSLDWRYGAAYFEQQLIDYDVSSNWGNWQYLAGVGADPRGHRQFNL 412

HSP 2 Score: 26.5646 bits (57), Expect = 1.067e-14
Identity = 11/36 (30.56%), Postives = 20/36 (55.56%), Query Frame = 1

Query:  535 PN*HPQF----EGYKFDPNGEYVRRWLPELARLPTE 630
            P  H QF    +  ++DP+ E++ RW  +L+  P +
Sbjct:  404 PRGHRQFNLEKQTERYDPDNEFIERWAGDLSGQPID 439

The following BLAST results are available for this feature:

BLAST of EY711062 vs. ExPASy Swiss-Prot

BLAST of EY711062 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 48


Match Name	E-value	Identity	Description
CRY1_SYLBO	5.847e-16	30.13	Cryptochrome-1 OS=Sylvia borin GN=CRY1 PE=2 SV=1	[more]
CRY1_ERIRU	5.847e-16	30.13	Cryptochrome-1 OS=Erithacus rubecula GN=CRY1 PE=2 ...	[more]
CRYD_OSTTA	7.731e-16	32.30	Cryptochrome DASH, chloroplastic/mitochondrial OS=...	[more]
CRY1_MOUSE	4.603e-15	30.13	Cryptochrome-1 OS=Mus musculus GN=Cry1 PE=1 SV=1	[more]
CRY1_RAT	4.609e-15	30.13	Cryptochrome-1 OS=Rattus norvegicus GN=Cry1 PE=2 S...	[more]
CRY1_HUMAN	4.610e-15	30.13	Cryptochrome-1 OS=Homo sapiens GN=CRY1 PE=1 SV=1	[more]
CRY1_SPAJD	7.714e-15	30.13	Cryptochrome-1 OS=Spalax judaei GN=CRY1 PE=2 SV=1	[more]
CRYD_GLOVI	8.186e-15	36.23	Cryptochrome DASH OS=Gloeobacter violaceus GN=cry ...	[more]
CRY1_MACFA	9.982e-15	29.49	Cryptochrome-1 OS=Macaca fascicularis GN=CRY1 PE=2...	[more]
CRYD_IDILO	1.067e-14	40.91	Cryptochrome DASH OS=Idiomarina loihiensis GN=cry ...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-702-003-D01-CT.F Sweet orange fruit, development stadium (3 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY711062 ID=EY711062; Name=EY711062; organism=Citrus sinensis; type=EST; length=891bp
CCACGCGTCCGCCCACGCGTCCGGTGCTACAACCTCATTTCTCTCTCCAC
ATTTGCATTTTGGGGAGGTTAGTGTGAGGAAAGTCTTCCATCTTGTCCGC
ATCAAGCAGGTTTCATGGGCCAATGAAGGGAACAAGGCTGGTGATGAGAG
TGTGAACTTGTTTCTCAAGTCAATTGGTCTTCGGGAATATTCAAGGTACA
TGAGTTTTAACCATCCTTACAGTCATGAAAGGCCTCTTCTTGGGCACCTT
AAGTTCTTTTCTTGGGTTGTGGATGAGGGCTATTTTAAGGCATGGAGGCA
AGGTAGAACTGGTTATCCACTGGTGGATGCTGGCATGAGAGAGTTGTGGG
CCACTGGTTGGCTTCATGATCGGATACGAGTAGTAGTTTCTAGTTTCTTT
GTAAAAGTTCTACAGCTTCCATGGAGATGGGGGATGAAATATTTTTGGGA
TACCCTCTTGGATGCAGATTTAGAAAGTGATGCTCTTGGTTGGCAGTACA
TATCCGGTACTATCCCTGATGGCCGTGCTTTTGACCGAATTGACATCCAC
AGTTTGAGGGTTACAAGTTTGACCCCAATGGAGAATATGTACGACGGTGG
CTCCCTGAACTTGCCAGACTGCCCACTGAATGGATACCCACCCATGGAAA
TGCCCAAAGTCTGGGCTCCAAGCTGCTGGAATGGAACTGGGGTCCCATTA
CCCTTTTCCCCTAAAACGGATTGGCGCCCCAAAATGCGGGGTGGACCAAA
CCCTTTCAAAATGTGGGCACAAAGAAACACCTTCCAAACCTTCATTTTAA
AAGGAACTGAGGAAAGGCCTTGGAAACTCCCTTTGATTCACCCCCAATTG
TCCTTTCCCCCGGAAAATAAAAGGGGGAGAAACCCCCAACA