EY710935

Overview
NameEY710935
Unique NameEY710935
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length922
Libraries
Library NameType
Sweet orange fruit, development stadium (3 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY710935 vs. ExPASy Swiss-Prot
Match: PPP6_HUMAN (Serine/threonine-protein phosphatase 6 catalytic subunit OS=Homo sapiens GN=PPP6C PE=1 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 2.818e-27
Identity = 55/108 (50.93%), Postives = 71/108 (65.74%), Query Frame = 3 
Query:  111 DLLWSDPDDRCGWGISPRGAGYTFGQDIASQFNHTNGLSLISRAHQLVMEGYNWCQDKNVVTVFSAPNYCYRCGNMAAIL---EIGENMEXNFLQFDPAPRQIEPDTT 425
            DL+WSDP+D   W ISPRGAG+ FG  + ++F H N L LI RAHQLV EGY +  D+ +VTV+SAPNYCYRCGN+A+I+   ++       F     + R I P TT
Sbjct:  193 DLVWSDPEDVDTWAISPRGAGWLFGAKVTNEFVHINNLKLICRAHQLVHEGYKFMFDEKLVTVWSAPNYCYRCGNIASIMVFKDVNTREPKLFRAVPDSERVIPPRTT 300

HSP 2 Score: 30.8018 bits (68), Expect = 2.818e-27
Identity = 14/26 (53.85%), Postives = 17/26 (65.38%), Query Frame = 2 
Query:   17 VRGGLSPSMDILDNIRALDRIQEGSH 94
            V GGLSP +  LD IR ++R QE  H
Sbjct:  162 VHGGLSPDIKTLDQIRTIERNQEIPH 187
[Close]
BLAST of EY710935 vs. ExPASy Swiss-Prot
Match: YD44_SCHPO (Putative serine/threonine-protein phosphatase C22H10.04 OS=Schizosaccharomyces pombe GN=SPAC22H10.04 PE=2 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 5.493e-25
Identity = 55/118 (46.61%), Postives = 73/118 (61.86%), Query Frame = 3 
Query:  105 MFDLLWSDPDDRCG-WGISPRGAGYTFGQDIASQFNHTNGLSLISRAHQLVMEGYNWCQDKNVVTVFSAPNYCYRCGNMAAILEIGENMEXNFLQFDPAPRQIEP---DTTRKTPDYF 446
            M DL+WSDPD     + +SPRGAG++FG+ I ++F   N +  I RAHQL  EGY    +K + TV+SAPNYCYRC N+A+IL+I  +    F  FD AP Q  P      + T +YF
Sbjct:  189 MADLVWSDPDPSVQEFSLSPRGAGFSFGEVIVTKFLEYNNMKHILRAHQLCSEGYQILFEKKLSTVWSAPNYCYRCANLASILQIDTDQSRFFNVFDAAPNQETPFVEPAAKVTAEYF 306

HSP 2 Score: 30.8018 bits (68), Expect = 5.493e-25
Identity = 14/26 (53.85%), Postives = 17/26 (65.38%), Query Frame = 2 
Query:   17 VRGGLSPSMDILDNIRALDRIQEGSH 94
            V GGLSPS+  +D I  LDR +E  H
Sbjct:  160 VHGGLSPSIQHIDQILVLDRFREFPH 185
[Close]
BLAST of EY710935 vs. ExPASy Swiss-Prot
Match: PPP6_DROME (Serine/threonine-protein phosphatase 6 catalytic subunit OS=Drosophila melanogaster GN=PpV PE=2 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 9.305e-25
Identity = 56/114 (49.12%), Postives = 70/114 (61.40%), Query Frame = 3 
Query:  111 DLLWSDPDDRCGWGISPRGAGYTFGQDIASQFNHTNGLSLISRAHQLVMEGYNWCQDKNVVTVFSAPNYCYRCGNMAAIL--EIGENMEXNFLQFDPAPRQIEPDTTRKTPDYF 446
            DL+WSDP+D   WG SPRGAG+ FG ++   F   N L+LI RAHQLV EG  +  D  +VTV+SAPNYCYRCGN+AAIL  E  E  +       P   ++ P   + T  YF
Sbjct:  191 DLVWSDPEDMEYWGQSPRGAGWLFGHNVTKDFMAINNLNLICRAHQLVNEGIKYMFDGKLVTVWSAPNYCYRCGNVAAILSFETAEKRQTKIFLAVPDAERVIP--KQNTTPYF 302

HSP 2 Score: 27.7202 bits (60), Expect = 9.305e-25
Identity = 12/20 (60.00%), Postives = 14/20 (70.00%), Query Frame = 2 
Query:   17 VRGGLSPSMDILDNIRALDR 76
            V GGLSP +  LD IR +DR
Sbjct:  160 VHGGLSPEIITLDQIRTIDR 179
[Close]
BLAST of EY710935 vs. ExPASy Swiss-Prot
Match: PPH6_CAEEL (Putative serine/threonine-protein phosphatase pph-6 OS=Caenorhabditis elegans GN=pph-6 PE=2 SV=2)

HSP 1 Score: 103.219 bits (256), Expect = 9.944e-24
Identity = 53/114 (46.49%), Postives = 70/114 (61.40%), Query Frame = 3 
Query:  111 DLLWSDPDDRC-GWGISPRGAGYTFGQDIASQFNHTNGLSLISRAHQLVMEGYNWCQDKNVVTVFSAPNYCYRCGNMAAILEI-GENMEXNFLQFDPAPRQIEPDTTRKTPDYF 446
            D++WSDPDD    W IS RGAG+ FG  +  +F   N LSL+ R+HQLV EG+ +  ++ + TV+SAPNYCYRCGN AA+ EI G N    +    P   + +PD  R    YF
Sbjct:  219 DIMWSDPDDDVEDWVISQRGAGFVFGAKVTEEFLMNNDLSLLCRSHQLVDEGFKYMFNEKLATVWSAPNYCYRCGNAAAVFEIDGNNRSTKYFNAVPDGSREKPD--RVVAPYF 330

HSP 2 Score: 28.4906 bits (62), Expect = 9.944e-24
Identity = 12/23 (52.17%), Postives = 16/23 (69.57%), Query Frame = 2 
Query:   17 VRGGLSPSMDILDNIRALDRIQE 85
            V GGLSP +  +D++  LDR QE
Sbjct:  188 VHGGLSPDIRTIDSLMLLDRAQE 210
[Close]
BLAST of EY710935 vs. ExPASy Swiss-Prot
Match: PP2A4_YEAST (Serine/threonine-protein phosphatase PP2A-like PPG1 OS=Saccharomyces cerevisiae GN=PPG1 PE=1 SV=2)

HSP 1 Score: 98.9821 bits (245), Expect = 4.816e-23
Identity = 54/120 (45.00%), Postives = 70/120 (58.33%), Query Frame = 3 
Query:   78 YRRVHIQRNMFDLLWSDPD----------DRCG--WGISPRGAGYTFGQDIASQFNHTNGLSLISRAHQLVMEGYNWCQDKNVVTVFSAPNYCYRCGNMAAILEIGENMEXNFLQFDPAP 401
            +R +     M DL+WSDP+          D  G  + +SPRGAGYTFG+ +  +F   N ++ I RAHQL  EGY    D  V TV+SAPNYCYRCGN A+ILE+    +  F  F+ AP
Sbjct:  180 FREIPHDGAMADLVWSDPEENNNPTLDHPDNSGQHFQVSPRGAGYTFGRSVVEKFLRMNDMNRIYRAHQLCNEGYQIYFDGLVTTVWSAPNYCYRCGNKASILELYSKDQFYFNVFEEAP 299

HSP 2 Score: 30.4166 bits (67), Expect = 4.816e-23
Identity = 12/26 (46.15%), Postives = 18/26 (69.23%), Query Frame = 2 
Query:   17 VRGGLSPSMDILDNIRALDRIQEGSH 94
            V GGLSP++  +D I+ +DR +E  H
Sbjct:  160 VHGGLSPNVQTIDQIKIIDRFREIPH 185
[Close]
BLAST of EY710935 vs. ExPASy Swiss-Prot
Match: PP14_ARATH (Serine/threonine-protein phosphatase PP1 isozyme 4 OS=Arabidopsis thaliana GN=TOPP4 PE=2 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 1.479e-21
Identity = 47/100 (47.00%), Postives = 64/100 (64.00%), Query Frame = 3 
Query:  111 DLLWSDPD-DRCGWGISPRGAGYTFGQDIASQFNHTNGLSLISRAHQLVMEGYNWCQDKNVVTVFSAPNYCYRCGNMAAILEIGENMEXNFLQFDPAPRQ 407
            DLLWSDP  D  GWG++ RG  YTFG D  S+F   + L L+ RAHQ+V +GY +  D+ +VTVFSAPNYC    N  A++ + EN+  +F    PA ++
Sbjct:  213 DLLWSDPGKDVKGWGMNDRGVSYTFGPDKVSEFLTKHDLDLVCRAHQVVEDGYEFFADRQLVTVFSAPNYCGEFDNAGAMMSVDENLMCSFQILKPAEKK 312

HSP 2 Score: 26.9498 bits (58), Expect = 1.479e-21
Identity = 12/20 (60.00%), Postives = 14/20 (70.00%), Query Frame = 2 
Query:   17 VRGGLSPSMDILDNIRALDR 76
            + GGLSP +D LD IR L R
Sbjct:  182 MHGGLSPDLDHLDEIRNLPR 201
[Close]
BLAST of EY710935 vs. ExPASy Swiss-Prot
Match: PP1_ORYSJ (Serine/threonine-protein phosphatase PP1 OS=Oryza sativa subsp. japonica GN=Os03g0268000 PE=2 SV=2)

HSP 1 Score: 97.0561 bits (240), Expect = 1.213e-20
Identity = 47/100 (47.00%), Postives = 63/100 (63.00%), Query Frame = 3 
Query:  111 DLLWSDPD-DRCGWGISPRGAGYTFGQDIASQFNHTNGLSLISRAHQLVMEGYNWCQDKNVVTVFSAPNYCYRCGNMAAILEIGENMEXNFLQFDPAPRQ 407
            DLLWSDP  D  GWG++ RG  YTFG D  S+F   + L LI RAHQ+V +GY +  D+ +VT+FSAPNYC    N  A++ + E +  +F    PA R+
Sbjct:  214 DLLWSDPGKDVQGWGMNDRGVSYTFGADKVSEFLEKHDLDLICRAHQVVEDGYEFFADRQLVTIFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPAERK 313

HSP 2 Score: 24.2534 bits (51), Expect = 1.213e-20
Identity = 10/20 (50.00%), Postives = 14/20 (70.00%), Query Frame = 2 
Query:   17 VRGGLSPSMDILDNIRALDR 76
            + GGLSP +  LD I++L R
Sbjct:  183 MHGGLSPDLTHLDEIKSLPR 202
[Close]
BLAST of EY710935 vs. ExPASy Swiss-Prot
Match: PP1_BRAOL (Serine/threonine-protein phosphatase PP1 OS=Brassica oleracea GN=PP1 PE=2 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 3.473e-20
Identity = 46/100 (46.00%), Postives = 63/100 (63.00%), Query Frame = 3 
Query:  111 DLLWSDPD-DRCGWGISPRGAGYTFGQDIASQFNHTNGLSLISRAHQLVMEGYNWCQDKNVVTVFSAPNYCYRCGNMAAILEIGENMEXNFLQFDPAPRQ 407
            DLLWSDP  D  GWG + RG  YTFG D  ++F   N + LI RAHQ+V +GY +  ++ VVTVFSAPNYC    N  A++ I E++  +F    P+ ++
Sbjct:  217 DLLWSDPSGDVKGWGANDRGVSYTFGADTVAEFLQKNDMDLICRAHQVVEDGYEFFAERQVVTVFSAPNYCGEFDNAGAMMSIDESLMCSFQILKPSEKK 316

HSP 2 Score: 23.8682 bits (50), Expect = 3.473e-20
Identity = 9/20 (45.00%), Postives = 14/20 (70.00%), Query Frame = 2 
Query:   17 VRGGLSPSMDILDNIRALDR 76
            + GG+SP +  LD IR++ R
Sbjct:  186 MHGGISPELMSLDQIRSISR 205
[Close]
BLAST of EY710935 vs. ExPASy Swiss-Prot
Match: PP11_SCHPO (Serine/threonine-protein phosphatase PP1-1 OS=Schizosaccharomyces pombe GN=dis2 PE=1 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 4.513e-20
Identity = 44/100 (44.00%), Postives = 65/100 (65.00%), Query Frame = 3 
Query:  111 DLLWSDPD-DRCGWGISPRGAGYTFGQDIASQFNHTNGLSLISRAHQLVMEGYNWCQDKNVVTVFSAPNYCYRCGNMAAILEIGENMEXNFLQFDPAPRQ 407
            DLLWSDPD D  GWG + RG  +TFG D+ S+F H + + L+ RAHQ+V +GY +   + +VT+FSAPNYC    N  A++ + E++  +F    PA ++
Sbjct:  202 DLLWSDPDKDLTGWGDNDRGVSFTFGPDVVSRFLHKHDMDLVCRAHQVVEDGYEFFSKRQLVTLFSAPNYCGEFDNAGAMMSVDESLLCSFQILKPAEKK 301

HSP 2 Score: 21.9422 bits (45), Expect = 4.513e-20
Identity = 8/20 (40.00%), Postives = 14/20 (70.00%), Query Frame = 2 
Query:   17 VRGGLSPSMDILDNIRALDR 76
            + GGLSP ++ +D I+ + R
Sbjct:  171 MHGGLSPDLNSMDQIQRIMR 190
[Close]
BLAST of EY710935 vs. ExPASy Swiss-Prot
Match: PP1G_RAT (Serine/threonine-protein phosphatase PP1-gamma catalytic subunit OS=Rattus norvegicus GN=Ppp1cc PE=1 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 5.871e-20
Identity = 47/107 (43.93%), Postives = 69/107 (64.49%), Query Frame = 3 
Query:  111 DLLWSDPD-DRCGWGISPRGAGYTFGQDIASQFNHTNGLSLISRAHQLVMEGYNWCQDKNVVTVFSAPNYCYRCGNMAAILEIGENMEXNFLQFDPAPRQIEPDTTR 428
            DLLWSDPD D  GWG + RG  +TFG ++ ++F H + L LI RAHQ+V +GY +   + +VT+FSAPNYC    N  A++ + E +  +F    PA ++ +P+ TR
Sbjct:  203 DLLWSDPDKDVLGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPAEKK-KPNATR 308

HSP 2 Score: 21.1718 bits (43), Expect = 5.871e-20
Identity = 8/18 (44.44%), Postives = 12/18 (66.67%), Query Frame = 2 
Query:   23 GGLSPSMDILDNIRALDR 76
            GGLSP +  ++ IR + R
Sbjct:  174 GGLSPDLQSMEQIRRIMR 191
The following BLAST results are available for this feature:
BLAST of EY710935 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 168
Match NameE-valueIdentityDescription
PPP6_HUMAN2.818e-2750.93Serine/threonine-protein phosphatase 6 catalytic s... [more]
YD44_SCHPO5.493e-2546.61Putative serine/threonine-protein phosphatase C22H... [more]
PPP6_DROME9.305e-2549.12Serine/threonine-protein phosphatase 6 catalytic s... [more]
PPH6_CAEEL9.944e-2446.49Putative serine/threonine-protein phosphatase pph-... [more]
PP2A4_YEAST4.816e-2345.00Serine/threonine-protein phosphatase PP2A-like PPG... [more]
PP14_ARATH1.479e-2147.00Serine/threonine-protein phosphatase PP1 isozyme 4... [more]
PP1_ORYSJ1.213e-2047.00Serine/threonine-protein phosphatase PP1 OS=Oryza ... [more]
PP1_BRAOL3.473e-2046.00Serine/threonine-protein phosphatase PP1 OS=Brassi... [more]
PP11_SCHPO4.513e-2044.00Serine/threonine-protein phosphatase PP1-1 OS=Schi... [more]
PP1G_RAT5.871e-2043.93Serine/threonine-protein phosphatase PP1-gamma cat... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-702-001-H01-CT.F Sweet orange fruit, development stadium (3 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY710935 ID=EY710935; Name=EY710935; organism=Citrus sinensis; type=EST; length=922bp
TTTCTCAACACCACGCGTCCGTGGAGGACTTTCCCCATCTATGGATATTC
TAGATAACATTAGGGCCCTGGACCGAATACAGGAGGGTTCACATCCAAAG
AAACATGTTTGATCTCTTGTGGTCTGATCCAGATGACCGCTGTGGGTGGG
GAATATCTCCCCGTGGTGCGGGCTATACATTTGGGCAGGATATAGCATCT
CAGTTCAACCACACCAATGGACTCAGTCTTATTTCAAGAGCCCACCAGCT
TGTCATGGAAGGATACAATTGGTGTCAGGATAAGAATGTGGTGACTGTAT
TTAGTGCTCCTAACTATTGTTACCGATGTGGGAATATGGCTGCAATTTTA
GAGATTGGGGAGAATATGGAGCANAATTTTCTCCAATTTGATCCAGCACC
ACGGCAAATAGAGCCTGACACCACACGCAAGACGCCTGATTATTTTTTTG
TAATTTCAATATTTCCACCATCTGTTTGTAATTCATGTTTGTTTACCTAC
CAGGGAGATGTGTCCTTAAAAAGGAGGAGCTTTTGTTTGTTAAGTTGAGG
GTACAACATCATTCTTTTGTTTCGAGGTTTGGCCTACTACTGCTTTGGTG
ATGGGCTGAAGGAGTCCATAGGAGTACTGACAGAAGGCATCAATGGGCTG
TTGGCTTGGATATTGGCCAAAGGGGGGGGAATAACATGTTATATCTTGGC
CTGGTGCGAAGACCAGCACGCGACACCGCAGGTTGCAGAGAGAAAAGAAA
AGGGCGGCCGCCCTAAAGGAACCAGGTTACGTAACGGGGGTGCGGAAGCA
AGAGCCCTCTAGAGGGGCCCTAAATCAATCCCCGGGGGGCGTTTTCAACG
CGGGGAGGGGAAAGCCGGGGGACCCACATTAAGCCCTGAAACCATCCCGT
TTCCCGAGGGGGGATAAGCGAG
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