EY710513

Overview
NameEY710513
Unique NameEY710513
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1081
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY710513 vs. ExPASy Swiss-Prot
Match: IF1A_DICDI (Eukaryotic translation initiation factor 1A OS=Dictyostelium discoideum GN=eif1a PE=3 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 1.459e-29
Identity = 62/85 (72.94%), Postives = 71/85 (83.53%), Query Frame = 1 
Query:  157 DDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLK 411
            +++KREL FKE+GQEYAQVLRMLGNGR EA C DG KRLCHI G++ KK WI  GDIIL+ LRDYQ+DKADVIL+Y  DEAR LK
Sbjct:   19 NEQKRELQFKEEGQEYAQVLRMLGNGRLEASCFDGEKRLCHISGRLRKKEWINNGDIILIQLRDYQNDKADVILRYNVDEARNLK 103

HSP 2 Score: 22.3274 bits (46), Expect = 1.459e-29
Identity = 8/11 (72.73%), Postives = 10/11 (90.91%), Query Frame = 2 
Query:  419 GELPESTRLNE 451
            GELPE+ R+NE
Sbjct:  106 GELPETARINE 116
[Close]
BLAST of EY710513 vs. ExPASy Swiss-Prot
Match: IF1A_YEAST (Eukaryotic translation initiation factor 1A OS=Saccharomyces cerevisiae GN=TIF11 PE=1 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 2.647e-28
Identity = 57/87 (65.52%), Postives = 70/87 (80.46%), Query Frame = 1 
Query:  151 EADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLK 411
            ++D  KREL++KE+GQEYAQ+ +MLGNGR EA C DG KR+ HIRGK+ KKVW+  GDIILV LRD+QDD+ DV+ KY  DEAR LK
Sbjct:   18 DSDGPKRELIYKEEGQEYAQITKMLGNGRVEASCFDGNKRMAHIRGKLRKKVWMGQGDIILVSLRDFQDDQCDVVHKYNLDEARTLK 104

HSP 2 Score: 21.1718 bits (43), Expect = 2.647e-28
Identity = 7/11 (63.64%), Postives = 10/11 (90.91%), Query Frame = 2 
Query:  419 GELPESTRLNE 451
            GELPE+ ++NE
Sbjct:  107 GELPENAKINE 117
[Close]
BLAST of EY710513 vs. ExPASy Swiss-Prot
Match: IF1A_SCHPO (Eukaryotic translation initiation factor 1A OS=Schizosaccharomyces pombe GN=tif11 PE=2 SV=2)

HSP 1 Score: 123.635 bits (309), Expect = 1.690e-27
Identity = 59/87 (67.82%), Postives = 69/87 (79.31%), Query Frame = 1 
Query:  151 EADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLK 411
            E ++EKREL + E+GQ YAQV +MLGNGR EA C DG KRL HIRGK+ KKVWI  GDIIL+ LR++QD+K DVILKY  DEAR LK
Sbjct:   18 ENENEKRELTYAEEGQMYAQVTKMLGNGRIEAACFDGVKRLGHIRGKLRKKVWINQGDIILLSLREFQDEKGDVILKYTADEARTLK 104

HSP 2 Score: 21.1718 bits (43), Expect = 1.690e-27
Identity = 7/11 (63.64%), Postives = 10/11 (90.91%), Query Frame = 2 
Query:  419 GELPESTRLNE 451
            GELPE+ ++NE
Sbjct:  107 GELPETAKINE 117
[Close]
BLAST of EY710513 vs. ExPASy Swiss-Prot
Match: IF1A_SULIY (Translation initiation factor 1A OS=Sulfolobus islandicus (strain Y.G.57.14 / Yellowstone #1) GN=eIF1A PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 3.969e-12
Identity = 32/80 (40.00%), Postives = 51/80 (63.75%), Query Frame = 1 
Query:  169 RELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLL 408
            R++   E+GQ    V +MLG      +C+DG +RL  I GK+ KK+W+  GD++LVG+ D+Q ++ D++ KY  DE + L
Sbjct:   13 RDVPKPEEGQTICVVKKMLGGDHLVVLCMDGKERLARIPGKIRKKMWMREGDVVLVGIWDFQPNRCDILYKYGNDEIKRL 92
[Close]
BLAST of EY710513 vs. ExPASy Swiss-Prot
Match: IF1A_SULIN (Translation initiation factor 1A OS=Sulfolobus islandicus (strain Y.N.15.51 / Yellowstone #2) GN=eIF1A PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 3.969e-12
Identity = 32/80 (40.00%), Postives = 51/80 (63.75%), Query Frame = 1 
Query:  169 RELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLL 408
            R++   E+GQ    V +MLG      +C+DG +RL  I GK+ KK+W+  GD++LVG+ D+Q ++ D++ KY  DE + L
Sbjct:   13 RDVPKPEEGQTICVVKKMLGGDHLVVLCMDGKERLARIPGKIRKKMWMREGDVVLVGIWDFQPNRCDILYKYGNDEIKRL 92
[Close]
BLAST of EY710513 vs. ExPASy Swiss-Prot
Match: IF1A_SULIM (Translation initiation factor 1A OS=Sulfolobus islandicus (strain M.14.25 / Kamchatka #1) GN=eIF1A PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 3.969e-12
Identity = 32/80 (40.00%), Postives = 51/80 (63.75%), Query Frame = 1 
Query:  169 RELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLL 408
            R++   E+GQ    V +MLG      +C+DG +RL  I GK+ KK+W+  GD++LVG+ D+Q ++ D++ KY  DE + L
Sbjct:   13 RDVPKPEEGQTICVVKKMLGGDHLVVLCMDGKERLARIPGKIRKKMWMREGDVVLVGIWDFQPNRCDILYKYGNDEIKRL 92
[Close]
BLAST of EY710513 vs. ExPASy Swiss-Prot
Match: IF1A_SULIL (Translation initiation factor 1A OS=Sulfolobus islandicus (strain L.S.2.15 / Lassen #1) GN=eIF1A PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 3.969e-12
Identity = 32/80 (40.00%), Postives = 51/80 (63.75%), Query Frame = 1 
Query:  169 RELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLL 408
            R++   E+GQ    V +MLG      +C+DG +RL  I GK+ KK+W+  GD++LVG+ D+Q ++ D++ KY  DE + L
Sbjct:   13 RDVPKPEEGQTICVVKKMLGGDHLVVLCMDGKERLARIPGKIRKKMWMREGDVVLVGIWDFQPNRCDILYKYGNDEIKRL 92
[Close]
BLAST of EY710513 vs. ExPASy Swiss-Prot
Match: IF1A_SULIK (Translation initiation factor 1A OS=Sulfolobus islandicus (strain M.16.4 / Kamchatka #3) GN=eIF1A PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 3.969e-12
Identity = 32/80 (40.00%), Postives = 51/80 (63.75%), Query Frame = 1 
Query:  169 RELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLL 408
            R++   E+GQ    V +MLG      +C+DG +RL  I GK+ KK+W+  GD++LVG+ D+Q ++ D++ KY  DE + L
Sbjct:   13 RDVPKPEEGQTICVVKKMLGGDHLVVLCMDGKERLARIPGKIRKKMWMREGDVVLVGIWDFQPNRCDILYKYGNDEIKRL 92
[Close]
BLAST of EY710513 vs. ExPASy Swiss-Prot
Match: IF1A_SULIA (Translation initiation factor 1A OS=Sulfolobus islandicus (strain M.16.27) GN=eIF1A PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 3.969e-12
Identity = 32/80 (40.00%), Postives = 51/80 (63.75%), Query Frame = 1 
Query:  169 RELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLL 408
            R++   E+GQ    V +MLG      +C+DG +RL  I GK+ KK+W+  GD++LVG+ D+Q ++ D++ KY  DE + L
Sbjct:   13 RDVPKPEEGQTICVVKKMLGGDHLVVLCMDGKERLARIPGKIRKKMWMREGDVVLVGIWDFQPNRCDILYKYGNDEIKRL 92
[Close]
BLAST of EY710513 vs. ExPASy Swiss-Prot
Match: IF1A_SULSO (Translation initiation factor 1A OS=Sulfolobus solfataricus GN=eIF1A PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 5.184e-12
Identity = 32/80 (40.00%), Postives = 51/80 (63.75%), Query Frame = 1 
Query:  169 RELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLL 408
            R++   E+GQ    V +MLG      +C+DG +RL  I GK+ KK+W+  GD++LVG+ D+Q ++ D++ KY  DE + L
Sbjct:   13 RDVPRPEEGQTICVVKKMLGGDHLIVLCMDGKERLARIPGKIRKKMWMREGDVVLVGIWDFQPNRCDILYKYGNDEIKRL 92
The following BLAST results are available for this feature:
BLAST of EY710513 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 25
Match NameE-valueIdentityDescription
IF1A_DICDI1.459e-2972.94Eukaryotic translation initiation factor 1A OS=Dic... [more]
IF1A_YEAST2.647e-2865.52Eukaryotic translation initiation factor 1A OS=Sac... [more]
IF1A_SCHPO1.690e-2767.82Eukaryotic translation initiation factor 1A OS=Sch... [more]
IF1A_SULIY3.969e-1240.00Translation initiation factor 1A OS=Sulfolobus isl... [more]
IF1A_SULIN3.969e-1240.00Translation initiation factor 1A OS=Sulfolobus isl... [more]
IF1A_SULIM3.969e-1240.00Translation initiation factor 1A OS=Sulfolobus isl... [more]
IF1A_SULIL3.969e-1240.00Translation initiation factor 1A OS=Sulfolobus isl... [more]
IF1A_SULIK3.969e-1240.00Translation initiation factor 1A OS=Sulfolobus isl... [more]
IF1A_SULIA3.969e-1240.00Translation initiation factor 1A OS=Sulfolobus isl... [more]
IF1A_SULSO5.184e-1240.00Translation initiation factor 1A OS=Sulfolobus sol... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-701-024-A11-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY710513 ID=EY710513; Name=EY710513; organism=Citrus sinensis; type=EST; length=1081bp
GGACCCGGGTTCCGGGATTCCCGGGTCGACCCCGCGTCCGTTCAATCTTG
CTGCTTTTTAACTCTCCGACTCCACCTCTCCCCCAAAGAATCAGAAACCA
TGCCGAAGAATAAGGGAAAGGGAGGAAAGAACAGGAAGAGGGGAAAGAAT
GAAGCTGACGACGAGAAGCGTGAGCTTGTCTTCAAGGAAGACGGACAGGA
ATATGCCCAAGTCCTTCGTATGCTCGGCAATGGTCGGTGTGAAGCTATGT
GCATCGATGGAACCAAGCGTCTTTGCCACATCCGTGGGAAGATGCACAAA
AAGGTTTGGATTGCAGCTGGTGATATAATACTTGTTGGCCTACGTGATTA
TCAGGATGACAAGGCTGATGTCATCCTCAAGTATATGCCCGACGAGGCAA
GGCTTCTGAAGGCTTAATGGTGAACTTCCAGAGAGTACCCGTCTCAATGA
GGGCATTGCTGGGTGGTCTTGATGAAAAGGATGATGGCGCTGATAATGAT
TACTTCGAGTTTGAAAACGAAGATATTGATAAGATCTAGGCTCCGAAAAA
CCGACCTCTGTCCGGGGGTTTAATGTTCAATATTTTAACAAAAAATGGAG
TATGCCAATGGAATTGGGTTAACTTAAAAACCTGAAAAAAAATGGGTTTG
AAGCCTTAAATTTTTGGAATTTTTAAAATGGGGGAGCCCTGCCATATTTT
CGGGGAAACGCCCAATAACCTTGGGTTAATTAATTTTTGTTTAATTCCCG
GGCCCTTTTTTTTGGAAAGGGGGATTTTTTCCAAAAAAGGGAAACCTTTA
AACCGGGGAGGGGGTGCAAAATTCATATAAAGGTCACAAATAAAAATATT
TGGGTGGGGGGAGTTTTCAAGGAAAAGACAGGGAAAAAAAAAATAAAAAA
AAAAAGGGGGGCGGGCGGGCCCTTTCATAGAGAGATCCGAAAGTATTTAC
ACGAGAGACGGGGGGGGAAGGGGGCAATACCATCCAAAATGCTCTTCTTT
TATTAATAGTAGGATCCCACACGAAATAATTTAACACCCTCTCCGCGGCG
GGGGGCGCGCCGCGCTACGCTTTTTATATCT
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