EY684133 | Citrus Genome Database

Overview


Name	EY684133
Unique Name	EY684133
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	862

Libraries


Library Name	Type
Sweet orange leaf, young greenhouse plant	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of EY684133 vs. ExPASy Swiss-Prot
Match: NEDD8_DROME (NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 3.120e-19
Identity = 45/79 (56.96%), Postives = 59/79 (74.68%), Query Frame = 2

Query:  158 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNILMESTLHLVLRLRGGMQI 394
            M I VKTLTGK I +++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +T ADY +   S LHLVL LRGG  I
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDSI 79

HSP 2 Score: 73.9442 bits (180), Expect = 1.270e-12
Identity = 35/77 (45.45%), Postives = 54/77 (70.13%), Query Frame = 2

Query:  386 MQIFVKTLTWKTITLEVESSDTIDNGKAKILDEEGIPPEQRRIFFAGEQLEDGRTLADYNLPKETPLPHVLRLRGGE 616
            M I VKTLT K I +++E +D +D  K ++ ++EGIPP+Q+R+ F+G+Q+ D +T ADY +   + L  VL LRGG+
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGD 77

HSP 3 Score: 70.8626 bits (172), Expect = 1.075e-11
Identity = 32/51 (62.75%), Postives = 40/51 (78.43%), Query Frame = 2

Query:   14 KIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQI 166
            ++++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG  I
Sbjct:   29 RVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDSI 79

BLAST of EY684133 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 5.323e-19
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 2

Query:  158 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNILMESTLHLVLRLRGG 385
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY IL  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 69.707 bits (169), Expect = 2.395e-11
Identity = 33/76 (43.42%), Postives = 53/76 (69.74%), Query Frame = 2

Query:  386 MQIFVKTLTWKTITLEVESSDTIDNGKAKILDEEGIPPEQRRIFFAGEQLEDGRTLADYNLPKETPLPHVLRLRGG 613
            M I VKTLT K I +++E +D ++  K ++ ++EGIPP+Q+R+ ++G+Q+ D +T ADY +   + L  VL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of EY684133 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 5.323e-19
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 2

Query:  158 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNILMESTLHLVLRLRGG 385
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY IL  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 69.707 bits (169), Expect = 2.395e-11
Identity = 33/76 (43.42%), Postives = 53/76 (69.74%), Query Frame = 2

Query:  386 MQIFVKTLTWKTITLEVESSDTIDNGKAKILDEEGIPPEQRRIFFAGEQLEDGRTLADYNLPKETPLPHVLRLRGG 613
            M I VKTLT K I +++E +D ++  K ++ ++EGIPP+Q+R+ ++G+Q+ D +T ADY +   + L  VL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of EY684133 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 95.1301 bits (235), Expect = 5.323e-19
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 2

Query:  158 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNILMESTLHLVLRLRGG 385
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY IL  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 69.707 bits (169), Expect = 2.395e-11
Identity = 33/76 (43.42%), Postives = 53/76 (69.74%), Query Frame = 2

Query:  386 MQIFVKTLTWKTITLEVESSDTIDNGKAKILDEEGIPPEQRRIFFAGEQLEDGRTLADYNLPKETPLPHVLRLRGG 613
            M I VKTLT K I +++E +D ++  K ++ ++EGIPP+Q+R+ ++G+Q+ D +T ADY +   + L  VL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of EY684133 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 5.323e-19
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 2

Query:  158 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNILMESTLHLVLRLRGG 385
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY IL  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 69.707 bits (169), Expect = 2.395e-11
Identity = 33/76 (43.42%), Postives = 53/76 (69.74%), Query Frame = 2

Query:  386 MQIFVKTLTWKTITLEVESSDTIDNGKAKILDEEGIPPEQRRIFFAGEQLEDGRTLADYNLPKETPLPHVLRLRGG 613
            M I VKTLT K I +++E +D ++  K ++ ++EGIPP+Q+R+ ++G+Q+ D +T ADY +   + L  VL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of EY684133 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 5.323e-19
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 2

Query:  158 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNILMESTLHLVLRLRGG 385
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY IL  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 69.707 bits (169), Expect = 2.395e-11
Identity = 33/76 (43.42%), Postives = 53/76 (69.74%), Query Frame = 2

Query:  386 MQIFVKTLTWKTITLEVESSDTIDNGKAKILDEEGIPPEQRRIFFAGEQLEDGRTLADYNLPKETPLPHVLRLRGG 613
            M I VKTLT K I +++E +D ++  K ++ ++EGIPP+Q+R+ ++G+Q+ D +T ADY +   + L  VL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of EY684133 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 94.3597 bits (233), Expect = 9.079e-19
Identity = 46/75 (61.33%), Postives = 58/75 (77.33%), Query Frame = 2

Query:  164 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNILMESTLHLVLRLRGGM 388
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I   S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

HSP 2 Score: 71.2478 bits (173), Expect = 8.231e-12
Identity = 36/74 (48.65%), Postives = 51/74 (68.92%), Query Frame = 2

Query:  392 IFVKTLTWKTITLEVESSDTIDNGKAKILDEEGIPPEQRRIFFAGEQLEDGRTLADYNLPKETPLPHVLRLRGG 613
            I VKTLT K I +++E +DTID  K ++ ++EGIPP Q+R+ +AG+QL D +T  DY +   + L  VL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGG 76

BLAST of EY684133 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.004e-17
Identity = 41/76 (53.95%), Postives = 57/76 (75.00%), Query Frame = 2

Query:  158 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNILMESTLHLVLRLRGG 385
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I   S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

HSP 2 Score: 71.2478 bits (173), Expect = 8.231e-12
Identity = 33/78 (42.31%), Postives = 51/78 (65.38%), Query Frame = 2

Query:  386 MQIFVKTLTWKTITLEVESSDTIDNGKAKILDEEGIPPEQRRIFFAGEQLEDGRTLADYNLPKETPLPHVLRLRGGEF 619
            + + V+TLT K I +++E +DT+D  K ++ + EGIPP Q+R+ + G+QL D +T  DY +   + L  VL LRGG F
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGGNF 78

BLAST of EY684133 vs. ExPASy Swiss-Prot
Match: NEDD8_DICDI (NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV=2)

HSP 1 Score: 87.4261 bits (215), Expect = 1.110e-16
Identity = 41/77 (53.25%), Postives = 57/77 (74.03%), Query Frame = 2

Query:  158 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNILMESTLHLVLRLRGGM 388
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I   S LHLVL LRGG+
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77

BLAST of EY684133 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 1.449e-16
Identity = 42/76 (55.26%), Postives = 55/76 (72.37%), Query Frame = 2

Query:  158 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNILMESTLHLVLRLRGG 385
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+   S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

HSP 2 Score: 71.2478 bits (173), Expect = 8.231e-12
Identity = 38/76 (50.00%), Postives = 51/76 (67.11%), Query Frame = 2

Query:  386 MQIFVKTLTWKTITLEVESSDTIDNGKAKILDEEGIPPEQRRIFFAGEQLEDGRTLADYNLPKETPLPHVLRLRGG 613
            M+I VKTLT K I +E+E +DTI+  K +I ++EGIPP  +RI + G+QL D  T   YNL + + L  VL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

The following BLAST results are available for this feature:

BLAST of EY684133 vs. ExPASy Swiss-Prot

BLAST of EY684133 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 94


Match Name	E-value	Identity	Description
NEDD8_DROME	3.120e-19	56.96	NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=...	[more]
NEDD8_RAT	5.323e-19	57.89	NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1	[more]
NEDD8_RABIT	5.323e-19	57.89	NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1	[more]
NEDD8_MOUSE	5.323e-19	57.89	NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2	[more]
NEDD8_HUMAN	5.323e-19	57.89	NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1	[more]
NEDD8_BOVIN	5.323e-19	57.89	NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1	[more]
RUB2_ARATH	9.079e-19	61.33	NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN...	[more]
RUB3_ORYSJ	1.004e-17	53.95	NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap...	[more]
NEDD8_DICDI	1.110e-16	53.25	NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV...	[more]
RUB3_ARATH	1.449e-16	55.26	NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-650-053-E01-CT.F Sweet orange leaf, young greenhouse plant Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY684133 ID=EY684133; Name=EY684133; organism=Citrus sinensis; type=EST; length=862bp
CCACGCGTCCGCTAAGATCCAGGACAAGGAAGGGATTCCACCGGACCAGC
AGCGGCTGATCTTCGCCGGAAAGCAGCTCGAGGACGGGCGCACCTTGGCG
GATTACAACATCCAGAAGGAGTCGACTCTCCACCTCGTCCTCCGTCTCCG
CGGCGGCATGCAGATCTTCGTCAAGACTCTGACCGGGAAGACGATCACCC
TGGAGGTGGAGAGCTCCGACACGATCGACAACGTGAAGGCGAAGATCCAG
GACAAGGAAGGGATCCCCCCGGACCAACAGAGGCTCATCTTCGCCGGCAA
GCAGCTGGAGGACGGCCGCACCCTGGCCGATTATAACATCCTGATGGAAT
CCACGCTCCACCTTGTGTTGCGCCTGAGGGGAGGAATGCAGATCTTCGTC
AAGACGTTGACCTGGAAGACGATTACCCTGGAGGTGGAGAGCTCCGACAC
GATCGACAACGGGAAGGCCAAGATCCTGGACGAGGAGGGGATTCCTCCGG
AACAGCGGAGGATCTTCTTCGCCGGAGAGCAGCTCGAGGATGGCCGGACT
CTCGCCGATTACAACCTTCCCAAGGAGACCCCTCTTCCCCATGTCCTCCG
TCTCCGAGGCGGGGAATTCTAATAAAATAATTGGGGAAGTGTTCTGCGTT
TTCATGGGAGGCGGCTAATAATACTACATTTGAGGAGGACAGAGTGGCTG
GAATAAAAATAATGTGGTAGAAAGAAGTGGAAGAATGGTAGCATGCATTT
AGGACTAATATTTCGCTATAGAATCAGCCGATAATAATAAATAAATAGAG
TATTCTAGATATTAAAAGAAAAGATGAGAGAGAGGCACTCGGGAGGAAGG
GAGGGAGTTTAA