EY679786

Overview
NameEY679786
Unique NameEY679786
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length954
Libraries
Library NameType
Sweet orange leaf, young greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY679786 vs. ExPASy Swiss-Prot
Match: ACCO4_PETHY (1-aminocyclopropane-1-carboxylate oxidase 4 OS=Petunia hybrida GN=ACO4 PE=3 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.207e-13
Identity = 52/177 (29.38%), Postives = 85/177 (48.02%), Query Frame = 3 
Query:    6 VVNHGIPLNVLEEIIEGIRKFNEQDVELKKEFYTRDRARNVRFNSNFDLHYSRTANWRDTLTISALASTDLDPNEYPEVCRDAVREYIKNVTKLGETLFELLSLALGLKAEHLQEIGCPKE---YTLPCHYYPSCPQPDLTLGATGHSDPSFLTILLQ-DQIGGLQVFHDNQWVGCP 524
            +VNHGIP  V++ + +  +   ++ +E + +     +          DL +  T   R     +     DLD +EY EV RD    + K + KL E L +LL   LGL+  +L++     +   +      YP CP+PDL  G   H+D   + +L Q D++ GLQ+  D+QW+  P
Sbjct:   36 LVNHGIPHEVMDTVEKFTKGHYKKCMEQRFKELVASKGLEAVQAEVTDLDWESTFFLRHLPVSNISEVPDLD-DEYREVMRD----FAKRLEKLAEELLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDAGGIILLFQDDKVSGLQLLKDDQWIDVP 207

HSP 2 Score: 25.0238 bits (53), Expect = 2.207e-13
Identity = 8/23 (34.78%), Postives = 18/23 (78.26%), Query Frame = 2 
Query:  527 PLWGGLVVNIGEFLEVVSNDQFQ 595
            P+   +V+N+G+ LEV++N +++
Sbjct:  208 PMRHSIVINLGDQLEVITNGKYK 230
[Close]
BLAST of EY679786 vs. ExPASy Swiss-Prot
Match: ACCO3_PETHY (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida GN=ACO3 PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 2.867e-13
Identity = 52/177 (29.38%), Postives = 84/177 (47.46%), Query Frame = 3 
Query:    6 VVNHGIPLNVLEEIIEGIRKFNEQDVELKKEFYTRDRARNVRFNSNFDLHYSRTANWRDTLTISALASTDLDPNEYPEVCRDAVREYIKNVTKLGETLFELLSLALGLKAEHLQEIGCPKE---YTLPCHYYPSCPQPDLTLGATGHSDPSFLTILLQ-DQIGGLQVFHDNQWVGCP 524
            +VNHGIP  V++ + +  +   ++ +E + +     +          DL +  T   R     +     DLD +EY EV RD    + K + KL E L +LL   LGL+  +L++     +   +      YP CP+PDL  G   H+D   + +L Q D++ GLQ+  D QW+  P
Sbjct:   36 LVNHGIPHEVMDTVEKLTKGHYKKCMEQRFKELVASKGLEAVQAEVTDLDWESTFFLRHLPVSNISEVPDLD-DEYREVMRD----FAKRLEKLAEELLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDAGGIILLFQDDKVSGLQLLKDGQWIDVP 207

HSP 2 Score: 25.409 bits (54), Expect = 2.867e-13
Identity = 9/23 (39.13%), Postives = 18/23 (78.26%), Query Frame = 2 
Query:  527 PLWGGLVVNIGEFLEVVSNDQFQ 595
            P+   +VVN+G+ LEV++N +++
Sbjct:  208 PMRHSIVVNLGDQLEVITNGKYK 230
[Close]
BLAST of EY679786 vs. ExPASy Swiss-Prot
Match: IDS2_HORVU (Mugineic-acid 3-dioxygenase OS=Hordeum vulgare GN=IDS2 PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 4.318e-13
Identity = 47/173 (27.17%), Postives = 73/173 (42.20%), Query Frame = 3 
Query:    3 RVVNHGIPLNVLEEIIEGIRKFNEQDVELKKEFYTRDRARNVRFNSNFDLHYSRTANWRDTLTIS-ALASTDL-DPNEYPEVCRDAVREYIKNVTKLGETLFELLSLALGLKAEHLQEIGCPKEYTLPCHYYPSCPQPDLTLGATGHSDPSFLTILLQDQIGGLQVFHDNQWV 515
            +VVNHGI   +L E+     +F +   E K EF++ DR+   +              W D L +   L S D  D    P++ R+ V  Y      +   +  LL   LGL+ +            +  +YYP  P P  TLG   H D   +T+LL   + GL++ +   W+
Sbjct:   62 QVVNHGISEPMLHEMYAVCHEFFDMPAEDKAEFFSEDRSERNKLFCGSAFETLGEKYWIDVLELLYPLPSGDTKDWPHKPQMLREVVGNYTSLARGVAMEILRLLCEGLGLRPDFFVGDISGGRVVVDINYYPPSPNPSRTLGLPPHCDRDLMTVLLPGAVPGLEIAYKGGWI 234

HSP 2 Score: 25.0238 bits (53), Expect = 4.318e-13
Identity = 12/25 (48.00%), Postives = 16/25 (64.00%), Query Frame = 2 
Query:  512 GWVS-HPLWGGLVVNIGEFLEVVSN 583
            GW+   P+   LV+N G  LEVV+N
Sbjct:  232 GWIKVQPVPNSLVINFGLQLEVVTN 256

HSP 3 Score: 21.9422 bits (45), Expect = 4.318e-13
Identity = 14/35 (40.00%), Postives = 17/35 (48.57%), Query Frame = 1 
Query:  634 PRGSRACFF-PGDIRQNPQSLMGPIKELTPEKNPP 735
            PR S A F  P D       ++GP +E   E NPP
Sbjct:  273 PRLSVASFIVPAD-----DCVVGPAEEFVSEDNPP 302
[Close]
BLAST of EY679786 vs. ExPASy Swiss-Prot
Match: ACCO_TOBAC (1-aminocyclopropane-1-carboxylate oxidase OS=Nicotiana tabacum GN=ACO PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 4.837e-13
Identity = 52/177 (29.38%), Postives = 85/177 (48.02%), Query Frame = 3 
Query:    6 VVNHGIPLNVLEEIIEGIRKFNEQDVELKKEFYTRDRARNVRFNSNFDLHYSRTANWRDTLTISALASTDLDPNEYPEVCRDAVREYIKNVTKLGETLFELLSLALGLKAEHLQEIGCPKE---YTLPCHYYPSCPQPDLTLGATGHSDPSFLTILLQ-DQIGGLQVFHDNQWVGCP 524
            +VNHGIP  V++ + +  +   ++ +E + +     +          DL +  T   R     +     DLD ++Y EV RD    + K + KL E L +LL   LGL+  +L++I    +   +      YP CP+PDL  G   H+D   + +L Q D++ GLQ+  D QW+  P
Sbjct:   36 LVNHGIPHEVMDTVEKMTKGHYKKCMEQRFKELVASKGLEAVQAEVTDLDWESTFFLRHLPVSNICEVPDLD-DQYREVMRD----FAKRLEKLAEELLDLLCENLGLEKGYLKKIFYGTKGPNFGSKVSNYPPCPKPDLIKGLRAHTDAGGIILLFQDDKVTGLQLLKDGQWIDVP 207

HSP 2 Score: 24.6386 bits (52), Expect = 4.837e-13
Identity = 9/23 (39.13%), Postives = 18/23 (78.26%), Query Frame = 2 
Query:  527 PLWGGLVVNIGEFLEVVSNDQFQ 595
            P+   +VVN+G+ LEV++N +++
Sbjct:  208 PMRLSIVVNLGDQLEVITNGKYK 230
[Close]
BLAST of EY679786 vs. ExPASy Swiss-Prot
Match: ACCO1_PETHY (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Petunia hybrida GN=ACO1 PE=1 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 4.837e-13
Identity = 48/179 (26.82%), Postives = 85/179 (47.49%), Query Frame = 3 
Query:    6 VVNHGIPLNVLEEIIEGIRKFNEQDVELK-KEFYTRDRARNVRFNSNFDLHYSRTANWRDTLTISALASTDL-DPNEYPEVCRDAVREYIKNVTKLGETLFELLSLALGLKAEHLQEIGCPKE---YTLPCHYYPSCPQPDLTLGATGHSDPSFLTILLQ-DQIGGLQVFHDNQWVGCP 524
            +VNHGIP  V++ + +  +   ++ +E + KE         V+             +W  T  +  L  +++ +  +  E  R+ +R++ K + KL E L +LL   LGL+  +L+      +   +      YP CP+PDL  G   H+D   + +L Q D++ GLQ+  D QW+  P
Sbjct:   36 LVNHGIPREVMDTVEKMTKGHYKKCMEQRFKELVASKALEGVQAEVT-------DMDWESTFFLKHLPISNISEVPDLDEEYREVMRDFAKRLEKLAEELLDLLCENLGLEKGYLKNAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDAGGIILLFQDDKVSGLQLLKDGQWIDVP 207

HSP 2 Score: 25.409 bits (54), Expect = 4.837e-13
Identity = 9/23 (39.13%), Postives = 18/23 (78.26%), Query Frame = 2 
Query:  527 PLWGGLVVNIGEFLEVVSNDQFQ 595
            P+   +VVN+G+ LEV++N +++
Sbjct:  208 PMRHSIVVNLGDQLEVITNGKYK 230
[Close]
BLAST of EY679786 vs. ExPASy Swiss-Prot
Match: ACCO_DENCR (1-aminocyclopropane-1-carboxylate oxidase OS=Dendrobium crumenatum GN=ACO PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 6.284e-13
Identity = 48/182 (26.37%), Postives = 84/182 (46.15%), Query Frame = 3 
Query:    6 VVNHGIPLNVLEEIIEGIRK--FNEQDVELKKEFYTRDRARNVRFNSNFDLHYSRTANWRDTLTISALASTDLDP-NEYPEVCRDAVREYIKNVTKLGETLFELLSLALGLKAEHLQEIGCPKEYTLPCH-----YYPSCPQPDLTLGATGHSDPSFLTILLQDQ-IGGLQVFHDNQWVGCP 524
            ++NHGI     E+  E + K  + E     +++ +    A+ +    N D       +W  T  +  L ++++    +  E C   ++E+   + KL E L +LL   LGL+  +L+ + C     LP        YP CP+P+L  G   H+D   + +L QD  + GLQ+  D +W+  P
Sbjct:   28 LLNHGISHEPNEQS-ETVNKEHYREHYRRFREQRFKEFAAKTLDSGENVD---GDNLDWESTFFLRHLPTSNISQVPDLDEDCGSTMKEFALGLEKLAERLLDLLCENLGLEKGYLKRVFCGGSDGLPTFGTKVSNYPPCPKPELIKGLRAHTDAGGIILLFQDDTVSGLQLLKDEEWIDVP 205

HSP 2 Score: 26.1794 bits (56), Expect = 6.284e-13
Identity = 10/23 (43.48%), Postives = 18/23 (78.26%), Query Frame = 2 
Query:  527 PLWGGLVVNIGEFLEVVSNDQFQ 595
            P+   +VVNIG+ LEV++N +++
Sbjct:  206 PMRHSIVVNIGDQLEVITNGKYK 228
[Close]
BLAST of EY679786 vs. ExPASy Swiss-Prot
Match: G2OX8_ARATH (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=2)

HSP 1 Score: 74.7146 bits (182), Expect = 8.670e-13
Identity = 53/173 (30.64%), Postives = 84/173 (48.55%), Query Frame = 3 
Query:    3 RVVNHGIPLNVLEEII-EGIRKFNEQ-DVELKKEFYTRDRARNVRFNSNFDLHYSRTANWRDTLTISALASTDLDPNEYPEVCRDAVREYIKNVTKLGETLFELLSLALGLKAEHLQEIGCPKEYTLPCHYYPSCPQPDLTLGATGHSDPSFLTILLQDQIGGLQVFHDNQWV 515
            +V+NHGI ++VLE++  E IR F E  D + K E ++   A + R+ +       R  +W +   +     TD+  N+        + ++      L   L E+L+   G  +   +E        L  + YP CP+P    G   H+D  FLTIL QDQ+GGLQ+  DN+W+
Sbjct:   76 QVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEKFS---AGSYRWGTPSATSI-RQLSWSEAFHVPM---TDISDNKDFTTLSSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLIKDNRWI 241
[Close]
BLAST of EY679786 vs. ExPASy Swiss-Prot
Match: GAO1D_WHEAT (Gibberellin 20 oxidase 1-D OS=Triticum aestivum GN=GA20ox1D PE=1 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.371e-12
Identity = 49/181 (27.07%), Postives = 82/181 (45.30%), Query Frame = 3 
Query:    3 RVVNHGIPLNVLEEIIEGIRKFNEQDVELKKEFYTRDRARNVRFNSNFDLHYSRTANWRDTLTISALASTDLDP-----------NEYPEVCRDAVREYIKNVTKLGETLFELLSLALGLKAEHLQEIGCPKEYTLPCHYYPSCPQPDLTLGATGHSDPSFLTILLQDQIGGLQVFHDNQW 512
            +VVNHGI   +L +    +  F    +  +K+   R    +  + S+F   ++    W++TL+  +  S   DP            E      +    Y   +++L   + E+L  +LG+   H +      +  +  +YYP C +P  TLG   H DP+ LTIL QD +GGLQV  + +W
Sbjct:   73 QVVNHGIDAELLADAHRCVDNFFTMPLP-EKQRALRHPGESCGYASSFTGRFASKLPWKETLSFRSCPS---DPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRW 249

HSP 2 Score: 22.7126 bits (47), Expect = 1.371e-12
Identity = 9/25 (36.00%), Postives = 14/25 (56.00%), Query Frame = 2 
Query:  527 PLWGGLVVNIGEFLEVVSNDQFQEC 601
            P     VVNIG+    +SN +++ C
Sbjct:  254 PRADAFVVNIGDTFMALSNGRYKSC 278
[Close]
BLAST of EY679786 vs. ExPASy Swiss-Prot
Match: GAO1B_WHEAT (Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.780e-12
Identity = 49/181 (27.07%), Postives = 81/181 (44.75%), Query Frame = 3 
Query:    3 RVVNHGIPLNVLEEIIEGIRKFNEQDVELKKEFYTRDRARNVRFNSNFDLHYSRTANWRDTLTISALASTDLDP-----------NEYPEVCRDAVREYIKNVTKLGETLFELLSLALGLKAEHLQEIGCPKEYTLPCHYYPSCPQPDLTLGATGHSDPSFLTILLQDQIGGLQVFHDNQW 512
            +VVNHGI   +L +    +  F    +  K+    R    +  + S+F   ++    W++TL+  +  S   DP            E      +    Y   +++L   + E+L  +LG+   H +      +  +  +YYP C +P  TLG   H DP+ LTIL QD +GGLQV  + +W
Sbjct:   73 QVVNHGIDAELLADAHRCVDAFFTMPLPEKQRALRRP-GESCGYASSFTGRFASKLPWKETLSFRSCPS---DPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAHYRRFFEGNDSIMRLNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRW 249

HSP 2 Score: 22.7126 bits (47), Expect = 1.780e-12
Identity = 9/25 (36.00%), Postives = 14/25 (56.00%), Query Frame = 2 
Query:  527 PLWGGLVVNIGEFLEVVSNDQFQEC 601
            P     VVNIG+    +SN +++ C
Sbjct:  254 PRADAFVVNIGDTFMALSNGRYKSC 278
[Close]
BLAST of EY679786 vs. ExPASy Swiss-Prot
Match: GAO1A_WHEAT (Gibberellin 20 oxidase 1-A OS=Triticum aestivum GN=GA20ox1A PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.780e-12
Identity = 49/181 (27.07%), Postives = 81/181 (44.75%), Query Frame = 3 
Query:    3 RVVNHGIPLNVLEEIIEGIRKFNEQDVELKKEFYTRDRARNVRFNSNFDLHYSRTANWRDTLTISALASTDLDP-----------NEYPEVCRDAVREYIKNVTKLGETLFELLSLALGLKAEHLQEIGCPKEYTLPCHYYPSCPQPDLTLGATGHSDPSFLTILLQDQIGGLQVFHDNQW 512
            +VVNHGI   +L +    +  F    +  K+    R    +  + S+F   ++    W++TL+  +  S   DP            E      +    Y   +++L   + E+L  +LG+   H +      +  +  +YYP C +P  TLG   H DP+ LTIL QD +GGLQV  + +W
Sbjct:   73 QVVNHGIDAELLADAHRCVDNFFTMPLPEKQRALRRP-GESCGYASSFTGRFASKLPWKETLSFRSCPS---DPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRW 249

HSP 2 Score: 22.7126 bits (47), Expect = 1.780e-12
Identity = 9/25 (36.00%), Postives = 14/25 (56.00%), Query Frame = 2 
Query:  527 PLWGGLVVNIGEFLEVVSNDQFQEC 601
            P     VVNIG+    +SN +++ C
Sbjct:  254 PRADAFVVNIGDTFMALSNGRYKSC 278
The following BLAST results are available for this feature:
BLAST of EY679786 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 65
Match NameE-valueIdentityDescription
ACCO4_PETHY2.207e-1329.381-aminocyclopropane-1-carboxylate oxidase 4 OS=Pet... [more]
ACCO3_PETHY2.867e-1329.381-aminocyclopropane-1-carboxylate oxidase 3 OS=Pet... [more]
IDS2_HORVU4.318e-1327.17Mugineic-acid 3-dioxygenase OS=Hordeum vulgare GN=... [more]
ACCO_TOBAC4.837e-1329.381-aminocyclopropane-1-carboxylate oxidase OS=Nicot... [more]
ACCO1_PETHY4.837e-1326.821-aminocyclopropane-1-carboxylate oxidase 1 OS=Pet... [more]
ACCO_DENCR6.284e-1326.371-aminocyclopropane-1-carboxylate oxidase OS=Dendr... [more]
G2OX8_ARATH8.670e-1330.64Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis th... [more]
GAO1D_WHEAT1.371e-1227.07Gibberellin 20 oxidase 1-D OS=Triticum aestivum GN... [more]
GAO1B_WHEAT1.780e-1227.07Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN... [more]
GAO1A_WHEAT1.780e-1227.07Gibberellin 20 oxidase 1-A OS=Triticum aestivum GN... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-650-004-A03-CT.F Sweet orange leaf, young greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY679786 ID=EY679786; Name=EY679786; organism=Citrus sinensis; type=EST; length=954bp
TTCGAGTGGTGAATCACGGTATTCCCTTAAATGTGTTGGAAGAGATTATT
GAAGGGATACGCAAGTTTAATGAACAAGACGTTGAGTTGAAGAAAGAGTT
TTATACCCGTGACCGGGCAAGAAATGTCAGATTCAACAGCAACTTTGATC
TGCATTACTCCCGGACAGCTAATTGGAGGGACACGTTGACAATTTCTGCT
TTAGCTTCCACCGATTTAGACCCCAACGAATACCCAGAAGTTTGCAGAGA
TGCTGTGAGAGAGTACATTAAAAATGTCACAAAATTGGGTGAAACTCTGT
TTGAATTATTATCACTGGCTCTTGGGCTGAAGGCTGAACATTTACAAGAG
ATTGGATGCCCTAAAGAATACACCCTTCCCTGCCACTACTATCCGTCTTG
CCCTCAGCCAGATTTGACATTGGGAGCTACCGGGCATTCAGACCCTTCAT
TTCTTACCATTCTTCTGCAAGACCAAATCGGCGGCCTTCAAGTCTTTCAT
GACAATCAATGGGTTGGGTGTCCCACCCATTGTGGGGAGGCTTAGTGGTA
AACATAGGGGAATTCCTCGAGGTTGTATCAAACGACCAGTTTCAGGAGTG
TTAACCCCAGAGTAATCGCCGGTCATGTCGGGTCCCAGGGGTTCCCGGGC
ATGCTTTTTTCCTGGGGACATAAGGCAGAATCCCCAAAGTCTTATGGGGC
CGATCAAAGAGTTGACACCGGAAAAAAATCCCCCCAATTTAGGGAAATTT
TCCTCCCAAGGAAAGGTTTTTGCAAAAGGAATTTTCCAGGGGGGGGGTGG
GAGGAACATTTCCCCCCCCCCCACAGCCCTAGGGAGTAGAGAACCCAGAA
CTATTTTGTGTTATAAATTTTCAGGGGGTTGGCTTCCCCAACCAAATTTC
AAAAATTTGCCGGAACCCCCGGCTGGGTGAACCTTTCTCTCCCCCGCACT
ATCT
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