EY679514

Overview
NameEY679514
Unique NameEY679514
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length945
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB_POLAG (Tubulin beta chain OS=Polytomella agilis GN=TUBB1 PE=3 SV=1)

HSP 1 Score: 279.641 bits (714), Expect = 1.757e-74
Identity = 142/201 (70.65%), Postives = 159/201 (79.10%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFVEYQR*NRKTGIK*WLLSSIFPFP*GS*NWC*T*QTTLLDNRLFENEDKRM 675
            MREI+HIQGGQCGNQIG+KFWEVV  EHGIDPTG   GD+DLQLERINVY+NEA+  RYVPRA+LMDLEPGTMDSVRSGPYGQ+FRPDNFVFGQ+GAGNNWAKGHYTEGAELIDSVLDVVRKEAE+CDCLQGFQVC SL GGTGSGMG  +   +   +   +  L  S+ P P  S         TL  ++L EN D+ M
Sbjct:    1 MREIVHIQGGQCGNQIGAKFWEVVSDEHGIDPTGTALGDSDLQLERINVYFNEATGSRYVPRAILMDLEPGTMDSVRSGPYGQIFRPDNFVFGQTGAGNNWAKGHYTEGAELIDSVLDVVRKEAESCDCLQGFQVCHSLGGGTGSGMGTLL-ISKIREEYPDRMMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENADECM 200
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB1_MANSE (Tubulin beta-1 chain OS=Manduca sexta PE=2 SV=1)

HSP 1 Score: 276.174 bits (705), Expect = 2.776e-74
Identity = 126/151 (83.44%), Postives = 140/151 (92.72%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFV 525
            MREI+HIQ GQCGNQIG+KFWE++  EHGIDPTG Y GD+DLQLERINVYYNEAS G+YVPRA+L+DLEPGTMDSVRSGP+GQ+FRPDNFVFGQSGAGNNWAKGHYTEGAEL+DSVLDVVRKEAE+CDCLQGFQ+  SL GGTGSGMG  +
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLL 151

HSP 2 Score: 25.0238 bits (53), Expect = 2.776e-74
Identity = 16/48 (33.33%), Postives = 24/48 (50.00%), Query Frame = 2 
Query:  587 PSPKAPETGVEPNKQHF*IIDYLKMKINGWVFDNEAL*INMVFRHHKI 730
            PSPK  +T VEP      +   ++     +  DNEAL  ++ FR  K+
Sbjct:  171 PSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL-YDICFRTLKL 217
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB_TRYCR (Tubulin beta chain OS=Trypanosoma cruzi PE=3 SV=2)

HSP 1 Score: 278.87 bits (712), Expect = 2.997e-74
Identity = 138/201 (68.66%), Postives = 160/201 (79.60%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFVEYQR*NRKTGIK*WLLSSIFPFP*GS*NWC*T*QTTLLDNRLFENEDKRM 675
            MREI+ +Q GQCGNQIGSKFWEV+  EHG+DPTG YQGD+DLQLERINVY++EA+ GRYVPRAVL+DLEPGTMDSVR+GPYGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELIDSVLDV RKEAE+CDCLQGFQ+C SL GGTGSGMG  +   +   +   +  +  SI P P  S        TTL  ++L EN D+ M
Sbjct:    1 MREIVCVQAGQCGNQIGSKFWEVISDEHGVDPTGTYQGDSDLQLERINVYFDEATGGRYVPRAVLIDLEPGTMDSVRAGPYGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELIDSVLDVCRKEAESCDCLQGFQICHSLGGGTGSGMGTLL-ISKLREEYPDRIMMTFSIIPSPKVSDTVVEPYNTTLSVHQLVENSDESM 200
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB_EUPCR (Tubulin beta chain OS=Euplotes crassus PE=3 SV=1)

HSP 1 Score: 278.87 bits (712), Expect = 2.997e-74
Identity = 139/201 (69.15%), Postives = 159/201 (79.10%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFVEYQR*NRKTGIK*WLLSSIFPFP*GS*NWC*T*QTTLLDNRLFENEDKRM 675
            MREI+H+QGGQCGNQIG+KFWEV+  EHG+DPTG Y GD+DLQLERINVYYNEA+ GRYVPRAVLMDLEPGTMDSVR+GP+GQ+FRPDNFVFGQ+GAGNNWAKGHYTEGAELIDSVLDVVRKEAE CDCLQGFQ+  SL GGTGSGMG  +   +   +   +     S+FP P  S         TL  ++L EN D+ M
Sbjct:    1 MREIVHVQGGQCGNQIGAKFWEVISDEHGVDPTGTYHGDSDLQLERINVYYNEATGGRYVPRAVLMDLEPGTMDSVRAGPFGQLFRPDNFVFGQTGAGNNWAKGHYTEGAELIDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGSGMGTLL-ISKIREEYPDRIMETFSVFPSPKVSDTVVEPYNATLSVHQLVENADEVM 200
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB1_NOTCO (Tubulin beta-1 chain OS=Notothenia coriiceps neglecta GN=tubb1 PE=1 SV=1)

HSP 1 Score: 276.174 bits (705), Expect = 3.621e-74
Identity = 127/151 (84.11%), Postives = 139/151 (92.05%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFV 525
            MREI+H+Q GQCGNQIGSKFWEV+  EHGIDPTG Y GD+DLQL+RINVYYNEAS G+YVPRAVL+DLEPGTMDSVRSGP+GQ+FRPDNFVFGQSGAGNNWAKGHYTEGAEL+DSVLDVVRKEAE CDCLQGFQ+  SL GGTGSGMG  +
Sbjct:    1 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGSYHGDSDLQLDRINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLL 151

HSP 2 Score: 24.6386 bits (52), Expect = 3.621e-74
Identity = 16/48 (33.33%), Postives = 24/48 (50.00%), Query Frame = 2 
Query:  587 PSPKAPETGVEPNKQHF*IIDYLKMKINGWVFDNEAL*INMVFRHHKI 730
            PSPK  +T VEP      +   ++     +  DNEAL  ++ FR  K+
Sbjct:  171 PSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEAL-YDICFRTLKL 217
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB4_XENLA (Tubulin beta-4 chain OS=Xenopus laevis GN=tubb4 PE=2 SV=1)

HSP 1 Score: 275.018 bits (702), Expect = 6.160e-74
Identity = 126/151 (83.44%), Postives = 140/151 (92.72%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFV 525
            MREI+H+Q GQCGNQIG+KFWEV+  EHGIDPTG Y GD+DLQLERINVYYNEA+ G+YVPRAVL+DLEPGTMDSVRSGP+GQ+FRPDNFVFGQSGAGNNWAKGHYTEGAEL+DSVLDVVRKEAE+CDCLQGFQ+  SL GGTGSGMG  +
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGAYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLL 151

HSP 2 Score: 25.0238 bits (53), Expect = 6.160e-74
Identity = 16/48 (33.33%), Postives = 24/48 (50.00%), Query Frame = 2 
Query:  587 PSPKAPETGVEPNKQHF*IIDYLKMKINGWVFDNEAL*INMVFRHHKI 730
            PSPK  +T VEP      +   ++     +  DNEAL  ++ FR  K+
Sbjct:  171 PSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL-YDICFRTLKL 217
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB2_ECHMU (Tubulin beta-2 chain OS=Echinococcus multilocularis GN=TUB-2 PE=3 SV=1)

HSP 1 Score: 275.018 bits (702), Expect = 6.160e-74
Identity = 125/151 (82.78%), Postives = 140/151 (92.72%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFV 525
            MREI+HIQ GQCGNQIG+KFWEV+  EHGIDPTG Y GD+DLQLERINVYYNEAS G+YVPRA+L+DLEPGTMDSVR+GP+GQ+FRPDNFVFGQSGAGNNWAKGHYTEGAEL+DSVLDV+RKEAE+CDCLQGFQ+  SL GGTGSGMG  +
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRAGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVIRKEAESCDCLQGFQLTHSLGGGTGSGMGTLL 151

HSP 2 Score: 25.0238 bits (53), Expect = 6.160e-74
Identity = 16/48 (33.33%), Postives = 24/48 (50.00%), Query Frame = 2 
Query:  587 PSPKAPETGVEPNKQHF*IIDYLKMKINGWVFDNEAL*INMVFRHHKI 730
            PSPK  +T VEP      +   ++     +  DNEAL  ++ FR  K+
Sbjct:  171 PSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL-YDICFRTLKL 217
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB2C_RAT (Tubulin beta-2C chain OS=Rattus norvegicus GN=Tubb2c PE=1 SV=1)

HSP 1 Score: 275.018 bits (702), Expect = 6.160e-74
Identity = 126/151 (83.44%), Postives = 140/151 (92.72%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFV 525
            MREI+H+Q GQCGNQIG+KFWEV+  EHGIDPTG Y GD+DLQLERINVYYNEA+ G+YVPRAVL+DLEPGTMDSVRSGP+GQ+FRPDNFVFGQSGAGNNWAKGHYTEGAEL+DSVLDVVRKEAE+CDCLQGFQ+  SL GGTGSGMG  +
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLL 151

HSP 2 Score: 25.0238 bits (53), Expect = 6.160e-74
Identity = 16/48 (33.33%), Postives = 24/48 (50.00%), Query Frame = 2 
Query:  587 PSPKAPETGVEPNKQHF*IIDYLKMKINGWVFDNEAL*INMVFRHHKI 730
            PSPK  +T VEP      +   ++     +  DNEAL  ++ FR  K+
Sbjct:  171 PSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL-YDICFRTLKL 217
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB2C_MOUSE (Tubulin beta-2C chain OS=Mus musculus GN=Tubb2c PE=1 SV=1)

HSP 1 Score: 275.018 bits (702), Expect = 6.160e-74
Identity = 126/151 (83.44%), Postives = 140/151 (92.72%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFV 525
            MREI+H+Q GQCGNQIG+KFWEV+  EHGIDPTG Y GD+DLQLERINVYYNEA+ G+YVPRAVL+DLEPGTMDSVRSGP+GQ+FRPDNFVFGQSGAGNNWAKGHYTEGAEL+DSVLDVVRKEAE+CDCLQGFQ+  SL GGTGSGMG  +
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLL 151

HSP 2 Score: 25.0238 bits (53), Expect = 6.160e-74
Identity = 16/48 (33.33%), Postives = 24/48 (50.00%), Query Frame = 2 
Query:  587 PSPKAPETGVEPNKQHF*IIDYLKMKINGWVFDNEAL*INMVFRHHKI 730
            PSPK  +T VEP      +   ++     +  DNEAL  ++ FR  K+
Sbjct:  171 PSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL-YDICFRTLKL 217
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB2C_HUMAN (Tubulin beta-2C chain OS=Homo sapiens GN=TUBB2C PE=1 SV=1)

HSP 1 Score: 275.018 bits (702), Expect = 6.160e-74
Identity = 126/151 (83.44%), Postives = 140/151 (92.72%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFV 525
            MREI+H+Q GQCGNQIG+KFWEV+  EHGIDPTG Y GD+DLQLERINVYYNEA+ G+YVPRAVL+DLEPGTMDSVRSGP+GQ+FRPDNFVFGQSGAGNNWAKGHYTEGAEL+DSVLDVVRKEAE+CDCLQGFQ+  SL GGTGSGMG  +
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLL 151

HSP 2 Score: 25.0238 bits (53), Expect = 6.160e-74
Identity = 16/48 (33.33%), Postives = 24/48 (50.00%), Query Frame = 2 
Query:  587 PSPKAPETGVEPNKQHF*IIDYLKMKINGWVFDNEAL*INMVFRHHKI 730
            PSPK  +T VEP      +   ++     +  DNEAL  ++ FR  K+
Sbjct:  171 PSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL-YDICFRTLKL 217
The following BLAST results are available for this feature:
BLAST of EY679514 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 416
Match NameE-valueIdentityDescription
TBB_POLAG1.757e-7470.65Tubulin beta chain OS=Polytomella agilis GN=TUBB1 ... [more]
TBB1_MANSE2.776e-7483.44Tubulin beta-1 chain OS=Manduca sexta PE=2 SV=1[more]
TBB_TRYCR2.997e-7468.66Tubulin beta chain OS=Trypanosoma cruzi PE=3 SV=2[more]
TBB_EUPCR2.997e-7469.15Tubulin beta chain OS=Euplotes crassus PE=3 SV=1[more]
TBB1_NOTCO3.621e-7484.11Tubulin beta-1 chain OS=Notothenia coriiceps negle... [more]
TBB4_XENLA6.160e-7483.44Tubulin beta-4 chain OS=Xenopus laevis GN=tubb4 PE... [more]
TBB2_ECHMU6.160e-7482.78Tubulin beta-2 chain OS=Echinococcus multiloculari... [more]
TBB2C_RAT6.160e-7483.44Tubulin beta-2C chain OS=Rattus norvegicus GN=Tubb... [more]
TBB2C_MOUSE6.160e-7483.44Tubulin beta-2C chain OS=Mus musculus GN=Tubb2c PE... [more]
TBB2C_HUMAN6.160e-7483.44Tubulin beta-2C chain OS=Homo sapiens GN=TUBB2C PE... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-052-A08-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY679514 ID=EY679514; Name=EY679514; organism=Citrus sinensis; type=EST; length=945bp
AAAAGAAAAGGAAGAATCCCCCCTTTTTATCCTCTGCAAATCTCGCTAAT
CAAGAAACCCCAACTTAAGAAAATGCGTGAAATCCTTCACATTCAAGGGG
GCCAATGCGGGAACCAAATCGGATCCAAGTTCTGGGAAGTGGTTTGCGCC
GAGCACGGCATAGACCCGACCGGAAAGTATCAAGGGGATACCGATTTGCA
GCTTGAGAGGATTAATGTGTATTACAACGAGGCAAGTTGCGGGAGATACG
TGCCTAGGGCCGTGCTCATGGATCTTGAACCTGGGACCATGGATAGCGTC
AGGTCTGGTCCGTACGGTCAGGTTTTTAGGCCCGACAATTTTGTCTTTGG
CCAGTCCGGCGCCGGAAACAACTGGGCGAAGGGGCATTATACTGAAGGAG
CTGAGCTGATTGATTCTGTGCTTGATGTTGTTCGTAAAGAAGCCGAGAAC
TGCGATTGCTTGCAGGGATTTCAGGTGTGCCCTTCTCTGGTAGGAGGGAC
GGGTTCCGGGATGGGAAACTTTGTTGAATATCAAAGATAAAATAGGAAAA
CCGGGATAAAATGATGGCTACTTTCCTCTATTTTCCCCTTCCCCTAAGGC
TCCTGAAACTGGTGTTGAACCTAACAAACAACACTTCTAGATAATCGACT
ATTTGAAAATGAAGATAAACGGATGGGTTTTTGATAATGAAGCCCTTTAA
ATAAATATGGTTTTCCGACACCATAAAATACAAATCCTCTAAAAATTGGG
AGGTAATCAACTAATATATACTCTAGATACAACGCCAAGAAATTAAATTT
GTTTTTTAATAAAACCCCGGATATAAATAATTTAACATTTTTTTAAAATA
TTAGTGAAAATGATCCATTATTCATTTTTAAAAAATTTACAAATTTTTTT
TCATGTAGGCGAAATATTGTCCCCTATTGACATAACTCCCTGTGT
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