EY679514

Overview
NameEY679514
Unique NameEY679514
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length945
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB4_BOVIN (Tubulin beta-4 chain OS=Bos taurus GN=TUBB4 PE=2 SV=1)

HSP 1 Score: 273.092 bits (697), Expect = 2.324e-73
Identity = 125/151 (82.78%), Postives = 139/151 (92.05%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFV 525
            MREI+H+Q GQCGNQIG+KFWEV+  EHGIDPTG Y GD+DLQLERINVYYNEA+ G YVPRAVL+DLEPGTMDSVRSGP+GQ+FRPDNFVFGQSGAGNNWAKGHYTEGAEL+D+VLDVVRKEAE+CDCLQGFQ+  SL GGTGSGMG  +
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGNYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLL 151

HSP 2 Score: 25.0238 bits (53), Expect = 2.324e-73
Identity = 16/48 (33.33%), Postives = 24/48 (50.00%), Query Frame = 2 
Query:  587 PSPKAPETGVEPNKQHF*IIDYLKMKINGWVFDNEAL*INMVFRHHKI 730
            PSPK  +T VEP      +   ++     +  DNEAL  ++ FR  K+
Sbjct:  171 PSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL-YDICFRTLKL 217
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB1_PHYPO (Tubulin beta-1 chain OS=Physarum polycephalum GN=BETA PE=1 SV=4)

HSP 1 Score: 273.863 bits (699), Expect = 3.198e-73
Identity = 125/151 (82.78%), Postives = 140/151 (92.72%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFV 525
            MREI+HIQ GQCGNQIG+KFWEV+  EHGIDPTG Y G++DLQLERINVYYNEA+ G+YVPRAVL+DLEPGTMDSVR+GP+GQ+FRPDNFVFGQ+GAGNNWAKGHYTEGAELIDSVLDVVRKEAE+CDCLQGFQ+  SL GGTGSGMG  +
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGESDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFVFGQTGAGNNWAKGHYTEGAELIDSVLDVVRKEAESCDCLQGFQIAHSLGGGTGSGMGTLL 151

HSP 2 Score: 23.0978 bits (48), Expect = 3.198e-73
Identity = 13/37 (35.14%), Postives = 17/37 (45.95%), Query Frame = 2 
Query:  587 PSPKAPETGVEPNKQHF*IIDYLKMKINGWVFDNEAL 697
            PSPK  +T VEP      +   ++        DNEAL
Sbjct:  171 PSPKVSDTVVEPYNATLSVHQLVENADEVMCIDNEAL 207

HSP 3 Score: 21.9422 bits (45), Expect = 3.198e-73
Identity = 10/16 (62.50%), Postives = 12/16 (75.00%), Query Frame = 3 
Query:  528 ISKIK*ENRDKMMATF 575
            ISKI+ E  D+MM TF
Sbjct:  152 ISKIREEYPDRMMCTF 167
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB_PIG (Tubulin beta chain OS=Sus scrofa PE=1 SV=1)

HSP 1 Score: 272.322 bits (695), Expect = 3.953e-73
Identity = 124/151 (82.12%), Postives = 139/151 (92.05%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFV 525
            MREI+HIQ GQCGNQIG+KFWEV+  EHGIDPTG Y GD+DLQLERINVYYNEA+  +YVPRA+L+DLEPGTMDSVRSGP+GQ+FRPDNFVFGQSGAGNNWAKGHYTEGAEL+DSVLDVVRKE+E+CDCLQGFQ+  SL GGTGSGMG  +
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAAGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLL 151

HSP 2 Score: 25.0238 bits (53), Expect = 3.953e-73
Identity = 16/48 (33.33%), Postives = 24/48 (50.00%), Query Frame = 2 
Query:  587 PSPKAPETGVEPNKQHF*IIDYLKMKINGWVFDNEAL*INMVFRHHKI 730
            PSPK  +T VEP      +   ++     +  DNEAL  ++ FR  K+
Sbjct:  171 PSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL-YDICFRTLKL 217
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB7_CHICK (Tubulin beta-7 chain OS=Gallus gallus PE=2 SV=1)

HSP 1 Score: 272.322 bits (695), Expect = 3.953e-73
Identity = 124/151 (82.12%), Postives = 140/151 (92.72%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFV 525
            MREI+HIQ GQCGNQIG+KFWEV+  EHGIDPTG Y GD+DLQL+RI+VYYNEA+ G+YVPRA+L+DLEPGTMDSVRSGP+GQ+FRPDNFVFGQSGAGNNWAKGHYTEGAEL+DSVLDVVRKEAE+CDCLQGFQ+  SL GGTGSGMG  +
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLL 151

HSP 2 Score: 25.0238 bits (53), Expect = 3.953e-73
Identity = 16/48 (33.33%), Postives = 24/48 (50.00%), Query Frame = 2 
Query:  587 PSPKAPETGVEPNKQHF*IIDYLKMKINGWVFDNEAL*INMVFRHHKI 730
            PSPK  +T VEP      +   ++     +  DNEAL  ++ FR  K+
Sbjct:  171 PSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL-YDICFRTLKL 217
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB5_XENTR (Tubulin beta chain OS=Xenopus tropicalis GN=tubb PE=2 SV=1)

HSP 1 Score: 272.322 bits (695), Expect = 3.953e-73
Identity = 124/151 (82.12%), Postives = 140/151 (92.72%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFV 525
            MREI+HIQ GQCGNQIG+KFWEV+  EHGIDPTG Y GD+DLQL+RI+VYYNEA+ G+YVPRA+L+DLEPGTMDSVRSGP+GQ+FRPDNFVFGQSGAGNNWAKGHYTEGAEL+DSVLDVVRKEAE+CDCLQGFQ+  SL GGTGSGMG  +
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLL 151

HSP 2 Score: 25.0238 bits (53), Expect = 3.953e-73
Identity = 16/48 (33.33%), Postives = 24/48 (50.00%), Query Frame = 2 
Query:  587 PSPKAPETGVEPNKQHF*IIDYLKMKINGWVFDNEAL*INMVFRHHKI 730
            PSPK  +T VEP      +   ++     +  DNEAL  ++ FR  K+
Sbjct:  171 PSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL-YDICFRTLKL 217
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB5_XENLA (Tubulin beta chain OS=Xenopus laevis GN=tubb PE=2 SV=1)

HSP 1 Score: 272.322 bits (695), Expect = 3.953e-73
Identity = 124/151 (82.12%), Postives = 140/151 (92.72%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFV 525
            MREI+HIQ GQCGNQIG+KFWEV+  EHGIDPTG Y GD+DLQL+RI+VYYNEA+ G+YVPRA+L+DLEPGTMDSVRSGP+GQ+FRPDNFVFGQSGAGNNWAKGHYTEGAEL+DSVLDVVRKEAE+CDCLQGFQ+  SL GGTGSGMG  +
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLL 151

HSP 2 Score: 25.0238 bits (53), Expect = 3.953e-73
Identity = 16/48 (33.33%), Postives = 24/48 (50.00%), Query Frame = 2 
Query:  587 PSPKAPETGVEPNKQHF*IIDYLKMKINGWVFDNEAL*INMVFRHHKI 730
            PSPK  +T VEP      +   ++     +  DNEAL  ++ FR  K+
Sbjct:  171 PSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL-YDICFRTLKL 217
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB5_RAT (Tubulin beta-5 chain OS=Rattus norvegicus GN=Tubb5 PE=1 SV=1)

HSP 1 Score: 272.322 bits (695), Expect = 3.953e-73
Identity = 124/151 (82.12%), Postives = 140/151 (92.72%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFV 525
            MREI+HIQ GQCGNQIG+KFWEV+  EHGIDPTG Y GD+DLQL+RI+VYYNEA+ G+YVPRA+L+DLEPGTMDSVRSGP+GQ+FRPDNFVFGQSGAGNNWAKGHYTEGAEL+DSVLDVVRKEAE+CDCLQGFQ+  SL GGTGSGMG  +
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLL 151

HSP 2 Score: 25.0238 bits (53), Expect = 3.953e-73
Identity = 16/48 (33.33%), Postives = 24/48 (50.00%), Query Frame = 2 
Query:  587 PSPKAPETGVEPNKQHF*IIDYLKMKINGWVFDNEAL*INMVFRHHKI 730
            PSPK  +T VEP      +   ++     +  DNEAL  ++ FR  K+
Sbjct:  171 PSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL-YDICFRTLKL 217
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB5_PONAB (Tubulin beta chain OS=Pongo abelii GN=TUBB PE=2 SV=1)

HSP 1 Score: 272.322 bits (695), Expect = 3.953e-73
Identity = 124/151 (82.12%), Postives = 140/151 (92.72%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFV 525
            MREI+HIQ GQCGNQIG+KFWEV+  EHGIDPTG Y GD+DLQL+RI+VYYNEA+ G+YVPRA+L+DLEPGTMDSVRSGP+GQ+FRPDNFVFGQSGAGNNWAKGHYTEGAEL+DSVLDVVRKEAE+CDCLQGFQ+  SL GGTGSGMG  +
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLL 151

HSP 2 Score: 25.0238 bits (53), Expect = 3.953e-73
Identity = 16/48 (33.33%), Postives = 24/48 (50.00%), Query Frame = 2 
Query:  587 PSPKAPETGVEPNKQHF*IIDYLKMKINGWVFDNEAL*INMVFRHHKI 730
            PSPK  +T VEP      +   ++     +  DNEAL  ++ FR  K+
Sbjct:  171 PSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL-YDICFRTLKL 217
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB5_PIG (Tubulin beta chain OS=Sus scrofa GN=TUBB PE=2 SV=1)

HSP 1 Score: 272.322 bits (695), Expect = 3.953e-73
Identity = 124/151 (82.12%), Postives = 140/151 (92.72%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFV 525
            MREI+HIQ GQCGNQIG+KFWEV+  EHGIDPTG Y GD+DLQL+RI+VYYNEA+ G+YVPRA+L+DLEPGTMDSVRSGP+GQ+FRPDNFVFGQSGAGNNWAKGHYTEGAEL+DSVLDVVRKEAE+CDCLQGFQ+  SL GGTGSGMG  +
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLL 151

HSP 2 Score: 25.0238 bits (53), Expect = 3.953e-73
Identity = 16/48 (33.33%), Postives = 24/48 (50.00%), Query Frame = 2 
Query:  587 PSPKAPETGVEPNKQHF*IIDYLKMKINGWVFDNEAL*INMVFRHHKI 730
            PSPK  +T VEP      +   ++     +  DNEAL  ++ FR  K+
Sbjct:  171 PSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL-YDICFRTLKL 217
[Close]
BLAST of EY679514 vs. ExPASy Swiss-Prot
Match: TBB5_PANTR (Tubulin beta chain OS=Pan troglodytes GN=TUBB PE=3 SV=1)

HSP 1 Score: 272.322 bits (695), Expect = 3.953e-73
Identity = 124/151 (82.12%), Postives = 140/151 (92.72%), Query Frame = 1 
Query:   73 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCPSLVGGTGSGMGNFV 525
            MREI+HIQ GQCGNQIG+KFWEV+  EHGIDPTG Y GD+DLQL+RI+VYYNEA+ G+YVPRA+L+DLEPGTMDSVRSGP+GQ+FRPDNFVFGQSGAGNNWAKGHYTEGAEL+DSVLDVVRKEAE+CDCLQGFQ+  SL GGTGSGMG  +
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLL 151

HSP 2 Score: 25.0238 bits (53), Expect = 3.953e-73
Identity = 16/48 (33.33%), Postives = 24/48 (50.00%), Query Frame = 2 
Query:  587 PSPKAPETGVEPNKQHF*IIDYLKMKINGWVFDNEAL*INMVFRHHKI 730
            PSPK  +T VEP      +   ++     +  DNEAL  ++ FR  K+
Sbjct:  171 PSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEAL-YDICFRTLKL 217
The following BLAST results are available for this feature:
BLAST of EY679514 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 416
Match NameE-valueIdentityDescription
TBB4_BOVIN2.324e-7382.78Tubulin beta-4 chain OS=Bos taurus GN=TUBB4 PE=2 S... [more]
TBB1_PHYPO3.198e-7382.78Tubulin beta-1 chain OS=Physarum polycephalum GN=B... [more]
TBB_PIG3.953e-7382.12Tubulin beta chain OS=Sus scrofa PE=1 SV=1[more]
TBB7_CHICK3.953e-7382.12Tubulin beta-7 chain OS=Gallus gallus PE=2 SV=1[more]
TBB5_XENTR3.953e-7382.12Tubulin beta chain OS=Xenopus tropicalis GN=tubb P... [more]
TBB5_XENLA3.953e-7382.12Tubulin beta chain OS=Xenopus laevis GN=tubb PE=2 ... [more]
TBB5_RAT3.953e-7382.12Tubulin beta-5 chain OS=Rattus norvegicus GN=Tubb5... [more]
TBB5_PONAB3.953e-7382.12Tubulin beta chain OS=Pongo abelii GN=TUBB PE=2 SV... [more]
TBB5_PIG3.953e-7382.12Tubulin beta chain OS=Sus scrofa GN=TUBB PE=2 SV=1[more]
TBB5_PANTR3.953e-7382.12Tubulin beta chain OS=Pan troglodytes GN=TUBB PE=3... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-052-A08-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY679514 ID=EY679514; Name=EY679514; organism=Citrus sinensis; type=EST; length=945bp
AAAAGAAAAGGAAGAATCCCCCCTTTTTATCCTCTGCAAATCTCGCTAAT
CAAGAAACCCCAACTTAAGAAAATGCGTGAAATCCTTCACATTCAAGGGG
GCCAATGCGGGAACCAAATCGGATCCAAGTTCTGGGAAGTGGTTTGCGCC
GAGCACGGCATAGACCCGACCGGAAAGTATCAAGGGGATACCGATTTGCA
GCTTGAGAGGATTAATGTGTATTACAACGAGGCAAGTTGCGGGAGATACG
TGCCTAGGGCCGTGCTCATGGATCTTGAACCTGGGACCATGGATAGCGTC
AGGTCTGGTCCGTACGGTCAGGTTTTTAGGCCCGACAATTTTGTCTTTGG
CCAGTCCGGCGCCGGAAACAACTGGGCGAAGGGGCATTATACTGAAGGAG
CTGAGCTGATTGATTCTGTGCTTGATGTTGTTCGTAAAGAAGCCGAGAAC
TGCGATTGCTTGCAGGGATTTCAGGTGTGCCCTTCTCTGGTAGGAGGGAC
GGGTTCCGGGATGGGAAACTTTGTTGAATATCAAAGATAAAATAGGAAAA
CCGGGATAAAATGATGGCTACTTTCCTCTATTTTCCCCTTCCCCTAAGGC
TCCTGAAACTGGTGTTGAACCTAACAAACAACACTTCTAGATAATCGACT
ATTTGAAAATGAAGATAAACGGATGGGTTTTTGATAATGAAGCCCTTTAA
ATAAATATGGTTTTCCGACACCATAAAATACAAATCCTCTAAAAATTGGG
AGGTAATCAACTAATATATACTCTAGATACAACGCCAAGAAATTAAATTT
GTTTTTTAATAAAACCCCGGATATAAATAATTTAACATTTTTTTAAAATA
TTAGTGAAAATGATCCATTATTCATTTTTAAAAAATTTACAAATTTTTTT
TCATGTAGGCGAAATATTGTCCCCTATTGACATAACTCCCTGTGT
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