EY679357 | Citrus Genome Database

Overview


Name	EY679357
Unique Name	EY679357
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	995

Libraries


Library Name	Type
Sweet orange leaf, infected with CiLV	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

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BLAST of EY679357 vs. ExPASy Swiss-Prot
Match: TRXB_MYCLE (Bifunctional thioredoxin reductase/thioredoxin OS=Mycobacterium leprae GN=trxB/A PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 3.399e-25
Identity = 40/73 (54.79%), Postives = 54/73 (73.97%), Query Frame = 2

Query:    8 LNLPREDEFWSRGISACAICDGASPLFKGQVLAVVGGGDTATEEAIYLTKFARHVHLLVRREQLRASRAMQDR 226
            L +P E E   RG+SACA CDG+   F+GQ +AV+GGGD+A EEA++LT+FAR V L+ RR++ RAS+ M  R
Sbjct:  126 LQIPGEQELLGRGVSACATCDGS--FFRGQDIAVIGGGDSAMEEALFLTRFARSVTLVHRRDEFRASKIMLGR 196

HSP 2 Score: 54.299 bits (129), Expect = 3.399e-25
Identity = 36/107 (33.64%), Postives = 50/107 (46.73%), Query Frame = 1

Query:  265 NITVHFNTETVDVVSNTKGQMSGILLRKVDTGEESVLKAKGLFYGIGHSPNSQLLQGQVELDSSGNVIC*GGYCKNFLLKVYLQLDMCRTTNGNKQ*LPAGSGCLAA 585
            N  + F T    V  N    ++G+ LR   TGEE+ L   G+F  IGH P S L+   V++D  G V+  G      +  V+   D+   T   +    AGSGC AA
Sbjct:  200 NDKIKFITNHTVVAVNGYTTVTGLRLRNTTTGEETTLVVTGVFVAIGHEPRSSLVSDVVDIDPDGYVLVKGRTTSTSMDGVFAAGDLVDRTY-RQAITAAGSGCAAA 305

[Close]

BLAST of EY679357 vs. ExPASy Swiss-Prot
Match: TRXB_MYCSM (Thioredoxin reductase OS=Mycobacterium smegmatis GN=trxB PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 7.548e-25
Identity = 36/75 (48.00%), Postives = 50/75 (66.67%), Query Frame = 2

Query:    2 KRLNLPREDEFWSRGISACAICDGASPLFKGQVLAVVGGGDTATEEAIYLTKFARHVHLLVRREQLRASRAMQDR 226
            + L +P E+     G+S CA CDG    F+ Q + VVGGGD+A EEA +LT+FAR V L+ RR++ RAS+ M +R
Sbjct:  119 RHLGVPGEEALTGMGVSTCATCDGF--FFRDQDIVVVGGGDSAMEEATFLTRFARSVTLIHRRDEFRASKIMLER 191

HSP 2 Score: 62.7734 bits (151), Expect = 7.548e-25
Identity = 43/109 (39.45%), Postives = 56/109 (51.38%), Query Frame = 1

Query:  259 NPNITVHFNTETVDVVSNTKGQMSGILLRKVDTGEESVLKAKGLFYGIGHSPNSQLLQGQVELDSSGNVIC*GGYCKNFLLKVYLQLDMCRTTNGNKQ*LPAGSGCLAA 585
            N  IT   NTE   +  + K  ++G+ LR   TGEES L   G+F  IGH P S+L++GQVELD  G V   G      L  V+   D+   T   +    AGSGC A+
Sbjct:  195 NEKITFLTNTEITQIEGDPK--VTGVRLRDTVTGEESKLDVTGVFVAIGHDPRSELVRGQVELDDEGYVKVQGRTTYTSLDGVFAAGDLVDHTY-RQAITAAGSGCAAS 300

[Close]

BLAST of EY679357 vs. ExPASy Swiss-Prot
Match: TRXB_RICFE (Thioredoxin reductase OS=Rickettsia felis GN=trxB PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.818e-24
Identity = 38/76 (50.00%), Postives = 49/76 (64.47%), Query Frame = 2

Query:    2 KRLNLPREDEFWSRGISACAICDGASPLFKGQVLAVVGGGDTATEEAIYLTKFARHVHLLVRREQLRASRAMQDRL 229
            K L++  E EF   G+SACA CDG    FK Q + VVGGG++A EEA+YLT  A  V +  RR+  RA + +QDRL
Sbjct:  117 KWLSIASEQEFRGFGVSACATCDGF--FFKNQEIVVVGGGNSAVEEALYLTNHASKVTITHRRDSFRAEKILQDRL 190

HSP 2 Score: 60.8474 bits (146), Expect = 2.818e-24
Identity = 37/119 (31.09%), Postives = 65/119 (54.62%), Query Frame = 1

Query:  247 RVFNNPNITVHFNTETVDVVSNTKGQ-MSGILLRKVDTGEESVLKAKGLFYGIGHSPNSQLLQGQVELDSSGNVIC*GGYCKNFLLKVYLQLDMCRTTNGNKQ*LPAGSGCLAAFVMGK 600
            R+F NP I+V ++    ++V + K + ++G+ ++ V T E S++   G+F  IGH+PN+ L  GQ+ +D    +I   G  K  +  V+   D+ +     +    AGSGC+AA  + K
Sbjct:  189 RLFKNPKISVIWDHVVDEIVGSDKPKSVTGVKIQNVHTKEISLVNCSGVFIAIGHAPNTGLFTGQIAMDDDNYIITKSGTTKTSVEGVFAAGDV-QDKIYRQAVTAAGSGCMAALEVEK 306

[Close]

BLAST of EY679357 vs. ExPASy Swiss-Prot
Match: TRXB_ECOLI (Thioredoxin reductase OS=Escherichia coli (strain K12) GN=trxB PE=1 SV=2)

HSP 1 Score: 75.8702 bits (185), Expect = 6.150e-24
Identity = 37/75 (49.33%), Postives = 51/75 (68.00%), Query Frame = 2

Query:    8 LNLPREDEFWSRGISACAICDGASPLFKGQVLAVVGGGDTATEEAIYLTKFARHVHLLVRREQLRASRAMQDRLV 232
            L LP E+ F  RG+SACA CDG    ++ Q +AV+GGG+TA EEA+YL+  A  VHL+ RR+  RA + +  RL+
Sbjct:  120 LGLPSEEAFKGRGVSACATCDGF--FYRNQKVAVIGGGNTAVEEALYLSNIASEVHLIHRRDGFRAEKILIKRLM 192

HSP 2 Score: 45.0542 bits (105), Expect = 6.150e-24
Identity = 24/66 (36.36%), Postives = 37/66 (56.06%), Query Frame = 1

Query:  259 NPNITVHFNTETVDVVSNTKGQMSGILLRKVDTGEE-SVLKAKGLFYGIGHSPNSQLLQGQVELDS 453
            N NI +H N    +V  +  G ++G+ LR     +    L   GLF  IGHSPN+ + +GQ+EL++
Sbjct:  197 NGNIILHTNRTLEEVTGDQMG-VTGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELEN 261

HSP 3 Score: 31.5722 bits (70), Expect = 6.150e-24
Identity = 13/22 (59.09%), Postives = 18/22 (81.82%), Query Frame = 3

Query:  495 SVEGVFAAGYVQDHEWKQAVTA 560
            S+ GVFAAG V DH ++QA+T+
Sbjct:  278 SIPGVFAAGDVMDHIYRQAITS 299

[Close]

BLAST of EY679357 vs. ExPASy Swiss-Prot
Match: TRXB_ECO57 (Thioredoxin reductase OS=Escherichia coli O157:H7 GN=trxB PE=3 SV=2)

HSP 1 Score: 75.8702 bits (185), Expect = 6.150e-24
Identity = 37/75 (49.33%), Postives = 51/75 (68.00%), Query Frame = 2

Query:    8 LNLPREDEFWSRGISACAICDGASPLFKGQVLAVVGGGDTATEEAIYLTKFARHVHLLVRREQLRASRAMQDRLV 232
            L LP E+ F  RG+SACA CDG    ++ Q +AV+GGG+TA EEA+YL+  A  VHL+ RR+  RA + +  RL+
Sbjct:  120 LGLPSEEAFKGRGVSACATCDGF--FYRNQKVAVIGGGNTAVEEALYLSNIASEVHLIHRRDGFRAEKILIKRLM 192

HSP 2 Score: 45.0542 bits (105), Expect = 6.150e-24
Identity = 24/66 (36.36%), Postives = 37/66 (56.06%), Query Frame = 1

Query:  259 NPNITVHFNTETVDVVSNTKGQMSGILLRKVDTGEE-SVLKAKGLFYGIGHSPNSQLLQGQVELDS 453
            N NI +H N    +V  +  G ++G+ LR     +    L   GLF  IGHSPN+ + +GQ+EL++
Sbjct:  197 NGNIILHTNRTLEEVTGDQMG-VTGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELEN 261

HSP 3 Score: 31.5722 bits (70), Expect = 6.150e-24
Identity = 13/22 (59.09%), Postives = 18/22 (81.82%), Query Frame = 3

Query:  495 SVEGVFAAGYVQDHEWKQAVTA 560
            S+ GVFAAG V DH ++QA+T+
Sbjct:  278 SIPGVFAAGDVMDHIYRQAITS 299

[Close]

BLAST of EY679357 vs. ExPASy Swiss-Prot
Match: TRXB_RICBR (Thioredoxin reductase OS=Rickettsia bellii (strain RML369-C) GN=trxB PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.052e-23
Identity = 37/74 (50.00%), Postives = 49/74 (66.22%), Query Frame = 2

Query:    8 LNLPREDEFWSRGISACAICDGASPLFKGQVLAVVGGGDTATEEAIYLTKFARHVHLLVRREQLRASRAMQDRL 229
            L +P E EF   G+SACA CDG    FK Q + VVGGG++A EEA+YLT  A  V ++ RR+  RA + +Q+RL
Sbjct:  119 LGIPTEQEFMGFGVSACATCDGF--FFKNQKVVVVGGGNSAVEEALYLTNHASKVTIVHRRDNFRAEKILQERL 190

HSP 2 Score: 57.3806 bits (137), Expect = 1.052e-23
Identity = 35/114 (30.70%), Postives = 61/114 (53.51%), Query Frame = 1

Query:  247 RVFNNPNITVHFNTETVDVVSNTKGQ-MSGILLRKVDTGEESVLKAKGLFYGIGHSPNSQLLQGQVELDSSGNVIC*GGYCKNFLLKVYLQLDMCRTTNGNKQ*LPAGSGCLAA 585
            R+F NP I+V ++    ++V N   + ++G+ ++ V T E S++   G+F  IGH PN+ L   QV +D+   +I   G  K  +  V+   D+ +     +    AG+GC+AA
Sbjct:  189 RLFKNPKISVIWDHVVEEIVGNNNPKSVTGVKIQNVHTKETSLVNCSGVFVAIGHKPNTALFAEQVTMDNDNYIITTPGSTKTNIEGVFAAGDV-QDKIYRQAITAAGTGCMAA 301

[Close]

BLAST of EY679357 vs. ExPASy Swiss-Prot
Match: TRXB_MYCTU (Thioredoxin reductase OS=Mycobacterium tuberculosis GN=trxB PE=1 SV=2)

HSP 1 Score: 78.5666 bits (192), Expect = 3.010e-23
Identity = 39/73 (53.42%), Postives = 52/73 (71.23%), Query Frame = 2

Query:    8 LNLPREDEFWSRGISACAICDGASPLFKGQVLAVVGGGDTATEEAIYLTKFARHVHLLVRREQLRASRAMQDR 226
            L +P E E   RG+S+CA CDG    F+ Q +AV+GGGD+A EEA +LT+FAR V L+ RR++ RAS+ M DR
Sbjct:  129 LQVPGEQELLGRGVSSCATCDGF--FFRDQDIAVIGGGDSAMEEATFLTRFARSVTLVHRRDEFRASKIMLDR 199

HSP 2 Score: 51.6026 bits (122), Expect = 3.010e-23
Identity = 37/113 (32.74%), Postives = 54/113 (47.79%), Query Frame = 1

Query:  247 RVFNNPNITVHFNTETVDVVSNTKGQMSGILLRKVDTGEESVLKAKGLFYGIGHSPNSQLLQGQVELDSSGNVIC*GGYCKNFLLKVYLQLDMCRTTNGNKQ*LPAGSGCLAA 585
            R  NN  I    N   V V  +T   ++G+ +R  +TG E+ L   G+F  IGH P S L++  +++D  G V+  G      L  V+   D+   T   +    AGSGC AA
Sbjct:  199 RARNNDKIRFLTNHTVVAVDGDTT--VTGLRVRDTNTGAETTLPVTGVFVAIGHEPRSGLVREAIDVDPDGYVLVQGRTTSTSLPGVFAAGDLVDRTY-RQAVTAAGSGCAAA 308

[Close]

BLAST of EY679357 vs. ExPASy Swiss-Prot
Match: TRXB_RICPR (Thioredoxin reductase OS=Rickettsia prowazekii GN=trxB PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 3.924e-23
Identity = 38/76 (50.00%), Postives = 49/76 (64.47%), Query Frame = 2

Query:    2 KRLNLPREDEFWSRGISACAICDGASPLFKGQVLAVVGGGDTATEEAIYLTKFARHVHLLVRREQLRASRAMQDRL 229
            K L +  E EF   G+S+CAICDG    FK Q + VVGGG++A EEA+YLT  A  V ++ RR   RA + +QDRL
Sbjct:  117 KWLGIASEQEFRGFGVSSCAICDGF--FFKNQEIVVVGGGNSALEEALYLTNHANKVTVVHRRNSFRAEKILQDRL 190

HSP 2 Score: 56.9954 bits (136), Expect = 3.924e-23
Identity = 33/119 (27.73%), Postives = 65/119 (54.62%), Query Frame = 1

Query:  247 RVFNNPNITVHFNTETVDVVSNTKGQ-MSGILLRKVDTGEESVLKAKGLFYGIGHSPNSQLLQGQVELDSSGNVIC*GGYCKNFLLKVYLQLDMCRTTNGNKQ*LPAGSGCLAAFVMGK 600
            R+F NP I+V ++    ++V + K + ++G+ ++ V T E +++   G+F  IGH+PN+ L +GQ+ +D    ++   G  +  +  V+   D+ +     +    A SGC+AA  + K
Sbjct:  189 RLFKNPKISVIWDHIIDEIVGSNKPKAVTGVKIQNVYTNEINLVNCSGVFIAIGHAPNTALFKGQIAIDDDNYIVTQSGSTRTNVEGVFAAGDV-QDKIYRQAVTAAASGCMAALEVAK 306

[Close]

BLAST of EY679357 vs. ExPASy Swiss-Prot
Match: TRXB_RICTY (Thioredoxin reductase OS=Rickettsia typhi GN=trxB PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 3.924e-23
Identity = 38/76 (50.00%), Postives = 49/76 (64.47%), Query Frame = 2

Query:    2 KRLNLPREDEFWSRGISACAICDGASPLFKGQVLAVVGGGDTATEEAIYLTKFARHVHLLVRREQLRASRAMQDRL 229
            K L +  E EF   G+S+CAICDG    FK Q + VVGGG++A EEA+YLT  A  V ++ RR   RA + +QDRL
Sbjct:  117 KWLGITSEQEFRGFGVSSCAICDGF--FFKNQDIVVVGGGNSALEEALYLTNHANKVTVVHRRNSFRAEKILQDRL 190

HSP 2 Score: 57.3806 bits (137), Expect = 3.924e-23
Identity = 35/119 (29.41%), Postives = 63/119 (52.94%), Query Frame = 1

Query:  247 RVFNNPNITVHFNTETVDVV-SNTKGQMSGILLRKVDTGEESVLKAKGLFYGIGHSPNSQLLQGQVELDSSGNVIC*GGYCKNFLLKVYLQLDMCRTTNGNKQ*LPAGSGCLAAFVMGK 600
            R+F NP I+V ++    ++V SN    ++G+ ++ V T E +++   G+F  IGH+PN+ L  GQ+ +D    +I   G  +  +  V+   D+ +     +    A SGC+AA  + K
Sbjct:  189 RLFKNPKISVIWDHVIDEIVGSNQPKTVTGVKIKNVYTNEINLVNCSGVFIAIGHTPNTTLFNGQIAIDDDNYIITQTGSTRTSVEGVFAAGDV-QDKIYRQAITAAASGCMAALEVAK 306

[Close]

BLAST of EY679357 vs. ExPASy Swiss-Prot
Match: TRXB_SPIBA (Thioredoxin reductase (Fragment) OS=Spironucleus barkhanus GN=TRXB PE=2 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 3.925e-23
Identity = 37/76 (48.68%), Postives = 52/76 (68.42%), Query Frame = 2

Query:    2 KRLNLPREDEFWSRGISACAICDGASPLFKGQVLAVVGGGDTATEEAIYLTKFARHVHLLVRREQLRASRAMQDRL 229
            +RL++  E +FW +G+SACA+CD  S + K +   VVGGGD A EEA YL+  A  V+L++RR+  RAS AM  R+
Sbjct:  111 QRLDVIGEKQFWQKGVSACAVCD--SMMAKNKDTVVVGGGDVACEEASYLSNIASKVYLILRRDAFRASAAMVQRV 184

HSP 2 Score: 55.4546 bits (132), Expect = 3.925e-23
Identity = 33/113 (29.20%), Postives = 64/113 (56.64%), Query Frame = 1

Query:  247 RVFNNPNITVHFNTETVDVVSNTKGQMSGILLRKVDTGEESVLKAKGLFYGIGHSPNSQLLQGQVELDSSGNVIC*GGYCKNFLLKVYLQLDMCRTTNGNKQ*LPAGSGCLAA 585
            RV +NP I + +N+   ++   T+  ++ IL++ + +G+ + LK + LF+ IGH+P ++LL+GQV++  +G ++         +  ++   D C      +  + AGSGC AA
Sbjct:  183 RVKSNPKIEIIYNSAVQEIKGETR--VNQILVKNLKSGDITPLKVEALFWCIGHTPQTRLLKGQVKMSENGYILVENQTQYTNVPGIFAAGDCCDWIY-RQAIVAAGSGCKAA 292

The following BLAST results are available for this feature:

BLAST of EY679357 vs. ExPASy Swiss-Prot

BLAST of EY679357 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 67


Match Name	E-value	Identity	Description
TRXB_MYCLE	3.399e-25	54.79	Bifunctional thioredoxin reductase/thioredoxin OS=...	[more]
TRXB_MYCSM	7.548e-25	48.00	Thioredoxin reductase OS=Mycobacterium smegmatis G...	[more]
TRXB_RICFE	2.818e-24	50.00	Thioredoxin reductase OS=Rickettsia felis GN=trxB ...	[more]
TRXB_ECOLI	6.150e-24	49.33	Thioredoxin reductase OS=Escherichia coli (strain ...	[more]
TRXB_ECO57	6.150e-24	49.33	Thioredoxin reductase OS=Escherichia coli O157:H7 ...	[more]
TRXB_RICBR	1.052e-23	50.00	Thioredoxin reductase OS=Rickettsia bellii (strain...	[more]
TRXB_MYCTU	3.010e-23	53.42	Thioredoxin reductase OS=Mycobacterium tuberculosi...	[more]
TRXB_RICPR	3.924e-23	50.00	Thioredoxin reductase OS=Rickettsia prowazekii GN=...	[more]
TRXB_RICTY	3.924e-23	50.00	Thioredoxin reductase OS=Rickettsia typhi GN=trxB ...	[more]
TRXB_SPIBA	3.925e-23	48.68	Thioredoxin reductase (Fragment) OS=Spironucleus b...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C1-401-059-C08-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY679357 ID=EY679357; Name=EY679357; organism=Citrus sinensis; type=EST; length=995bp
CAAAAGGCTCAATTTACCTCGTGAAGATGAGTTTTGGAGTAGAGGAATCA
GTGCTTGTGCAATATGTGATGGAGCATCTCCCCTGTTTAAAGGGCAAGTT
CTTGCCGTGGTTGGAGGAGGTGATACAGCTACAGAGGAAGCAATATACTT
AACGAAATTTGCTCGTCATGTTCATTTACTTGTTCGTAGGGAACAGCTAA
GGGCATCCAGAGCAATGCAAGATAGGTTGGTTATCGCGAATGTTTCAGAG
TGTTCAACAACCCAAATATCACTGTGCACTTCAATACGGAGACAGTTGAT
GTTGTCAGCAATACAAAAGGCCAAATGTCCGGCATTTTACTTAGAAAAGT
TGACACTGGTGAGGAATCTGTGCTAAAGGCAAAAGGCTTATTTTATGGTA
TAGGTCATTCACCGAATAGCCAGTTGCTGCAAGGCCAAGTTGAACTTGAC
AGCTCAGGTAATGTGATTTGTTGAGGAGGGTACTGCAAAAACTTTCTGTT
GAAGGTGTATTTGCAGCTGGATATGTGCAGGACCACGAATGGAAACAAGC
AGTAACTGCCTGCTGGATCAGGATGCCTTGCTGCTTTTGTCATGGGAAAA
ATACCTTGGGAAACATTACTCTCCTTATTGAGTTTTGCTCAGCCCTCTAA
CCTTGAATATCCTCACATGGATCTTACTGATTAAAAATGTTCTAATAATG
GGTTCGAAACCTTTTGACATCAAGGATATGGGACAAATTATGGACCCTAA
AAGATAGTAAACCAAGAAGGTTCTAAGGCTTAATAAGGTTACAAATAAAA
TTTTAGCAAATTTGGCGGGTACACATCCACAGAATATTTAAAAGCCCAAG
AACATGGGCAAGCGGAAGACCACAGGTTATTTGTGAAAAGTAAACATGGT
ACATAATTATTAGAATATGAGTACAAAAATGTATTAAAAGAATATATCAA
TGACCGATTAACAGATAATGTACTAAACATATTTCCAATATTATG