EY679271 | Citrus Genome Database

Overview


Name	EY679271
Unique Name	EY679271
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	999

Libraries


Library Name	Type
Sweet orange leaf, infected with CiLV	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY679271 vs. ExPASy Swiss-Prot
Match: MK03_RAT (Mitogen-activated protein kinase 3 OS=Rattus norvegicus GN=Mapk3 PE=1 SV=5)

HSP 1 Score: 125.176 bits (313), Expect = 7.275e-31
Identity = 58/121 (47.93%), Postives = 87/121 (71.90%), Query Frame = 3

Query:    6 RILREVKLLRLLRHPDIVEIKRIMLPPSKREFKDIYVVFELMESDLHQVIKANDDLTREHHQFFLYQMLRALKYMHTANVYHRDLKPKNILANANCKLKVCDFGLARVAFSDTPMTVFWTE 368
            R LRE+++L   RH +++ I+ I+  P+    +D+Y+V +LME+DL++++K+   L+ +H  +FLYQ+LR LKY+H+ANV HRDLKP N+L N  C LK+CDFGLAR+A  +   T F TE
Sbjct:   85 RTLREIQILLRFRHENVIGIRDILRAPTLEAMRDVYIVQDLMETDLYKLLKSQQ-LSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPEHDHTGFLTE 204

HSP 2 Score: 30.8018 bits (68), Expect = 7.275e-31
Identity = 12/16 (75.00%), Postives = 13/16 (81.25%), Query Frame = 1

Query:  358 FGRNYVATRWYRAPEV 405
            F   YVATRWYRAPE+
Sbjct:  201 FLTEYVATRWYRAPEI 216

[Close]

BLAST of EY679271 vs. ExPASy Swiss-Prot
Match: MK03_MOUSE (Mitogen-activated protein kinase 3 OS=Mus musculus GN=Mapk3 PE=1 SV=5)

HSP 1 Score: 125.176 bits (313), Expect = 7.275e-31
Identity = 58/121 (47.93%), Postives = 87/121 (71.90%), Query Frame = 3

Query:    6 RILREVKLLRLLRHPDIVEIKRIMLPPSKREFKDIYVVFELMESDLHQVIKANDDLTREHHQFFLYQMLRALKYMHTANVYHRDLKPKNILANANCKLKVCDFGLARVAFSDTPMTVFWTE 368
            R LRE+++L   RH +++ I+ I+  P+    +D+Y+V +LME+DL++++K+   L+ +H  +FLYQ+LR LKY+H+ANV HRDLKP N+L N  C LK+CDFGLAR+A  +   T F TE
Sbjct:   85 RTLREIQILLRFRHENVIGIRDILRAPTLEAMRDVYIVQDLMETDLYKLLKSQQ-LSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPEHDHTGFLTE 204

HSP 2 Score: 30.8018 bits (68), Expect = 7.275e-31
Identity = 12/16 (75.00%), Postives = 13/16 (81.25%), Query Frame = 1

Query:  358 FGRNYVATRWYRAPEV 405
            F   YVATRWYRAPE+
Sbjct:  201 FLTEYVATRWYRAPEI 216

[Close]

BLAST of EY679271 vs. ExPASy Swiss-Prot
Match: SUR1_CAEEL (Mitogen-activated protein kinase mpk-1 OS=Caenorhabditis elegans GN=mpk-1 PE=1 SV=2)

HSP 1 Score: 123.25 bits (308), Expect = 2.716e-30
Identity = 61/121 (50.41%), Postives = 83/121 (68.60%), Query Frame = 3

Query:    6 RILREVKLLRLLRHPDIVEIKRIMLPPSKREFKDIYVVFELMESDLHQVIKANDDLTREHHQFFLYQMLRALKYMHTANVYHRDLKPKNILANANCKLKVCDFGLARVAFSDTPMTVFWTE 368
            R LRE+K+L   +H +I+ I+ I+   +    KDIY+V  LME+DL++++K    L+ +H  +FLYQ+LR LKY+H+ANV HRDLKP N+L N  C LK+CDFGLARV    T  T F TE
Sbjct:  138 RTLREIKILNRFKHENIINIQEIIRSETVDSLKDIYIVQCLMETDLYKLLKTQK-LSNDHVCYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVTDPQTDHTGFLTE 257

HSP 2 Score: 30.8018 bits (68), Expect = 2.716e-30
Identity = 12/16 (75.00%), Postives = 13/16 (81.25%), Query Frame = 1

Query:  358 FGRNYVATRWYRAPEV 405
            F   YVATRWYRAPE+
Sbjct:  254 FLTEYVATRWYRAPEI 269

[Close]

BLAST of EY679271 vs. ExPASy Swiss-Prot
Match: MPK12_ARATH (Mitogen-activated protein kinase 12 OS=Arabidopsis thaliana GN=MPK12 PE=1 SV=1)

HSP 1 Score: 124.405 bits (311), Expect = 4.619e-30
Identity = 56/113 (49.56%), Postives = 85/113 (75.22%), Query Frame = 3

Query:    6 RILREVKLLRLLRHPDIVEIKRIMLPPSKREFKDIYVVFELMESDLHQVIKANDDLTREHHQFFLYQMLRALKYMHTANVYHRDLKPKNILANANCKLKVCDFGLARVAFSDT 344
            R LRE+KLLR + H +++ IK I+ PP +  F D+Y+V+ELM++DL +++++N  LT +  +F +YQ+LR LKY+H+AN+ HRDL+P N+L N+  +LK+ DFGLAR   SDT
Sbjct:   84 RTLREIKLLRHMDHENVITIKDIVRPPQRDIFNDVYIVYELMDTDLQRILRSNQTLTSDQCRFLVYQLLRGLKYVHSANILHRDLRPSNVLLNSKNELKIGDFGLARTT-SDT 195

HSP 2 Score: 28.8758 bits (63), Expect = 4.619e-30
Identity = 11/16 (68.75%), Postives = 12/16 (75.00%), Query Frame = 1

Query:  358 FGRNYVATRWYRAPEV 405
            F   YV TRWYRAPE+
Sbjct:  197 FMTEYVVTRWYRAPEL 212

[Close]

BLAST of EY679271 vs. ExPASy Swiss-Prot
Match: FUS3_YEAST (Mitogen-activated protein kinase FUS3 OS=Saccharomyces cerevisiae GN=FUS3 PE=1 SV=2)

HSP 1 Score: 122.479 bits (306), Expect = 4.623e-30
Identity = 56/109 (51.38%), Postives = 81/109 (74.31%), Query Frame = 3

Query:    3 IRILREVKLLRLLRHPDIVEIKRIMLPPSKREFKDIYVVFELMESDLHQVIKANDDLTREHHQFFLYQMLRALKYMHTANVYHRDLKPKNILANANCKLKVCDFGLARV 329
            +R LRE+K+L+  +H +I+ I  I  P S   F ++Y++ ELM++DLH+VI +   L+ +H Q+F+YQ LRA+K +H +NV HRDLKP N+L N+NC LKVCDFGLAR+
Sbjct:   54 LRTLREIKILKHFKHENIITIFNIQRPDSFENFNEVYIIQELMQTDLHRVI-STQMLSDDHIQYFIYQTLRAVKVLHGSNVIHRDLKPSNLLINSNCDLKVCDFGLARI 161

HSP 2 Score: 30.8018 bits (68), Expect = 4.623e-30
Identity = 12/12 (100.00%), Postives = 12/12 (100.00%), Query Frame = 1

Query:  370 YVATRWYRAPEV 405
            YVATRWYRAPEV
Sbjct:  182 YVATRWYRAPEV 193

[Close]

BLAST of EY679271 vs. ExPASy Swiss-Prot
Match: MK03_HUMAN (Mitogen-activated protein kinase 3 OS=Homo sapiens GN=MAPK3 PE=1 SV=4)

HSP 1 Score: 122.094 bits (305), Expect = 6.012e-30
Identity = 57/121 (47.11%), Postives = 86/121 (71.07%), Query Frame = 3

Query:    6 RILREVKLLRLLRHPDIVEIKRIMLPPSKREFKDIYVVFELMESDLHQVIKANDDLTREHHQFFLYQMLRALKYMHTANVYHRDLKPKNILANANCKLKVCDFGLARVAFSDTPMTVFWTE 368
            R LRE+++L   RH +++ I+ I+   +    +D+Y+V +LME+DL++++K+   L+ +H  +FLYQ+LR LKY+H+ANV HRDLKP N+L N  C LK+CDFGLAR+A  +   T F TE
Sbjct:   84 RTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYIVQDLMETDLYKLLKSQQ-LSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPEHDHTGFLTE 203

HSP 2 Score: 30.8018 bits (68), Expect = 6.012e-30
Identity = 12/16 (75.00%), Postives = 13/16 (81.25%), Query Frame = 1

Query:  358 FGRNYVATRWYRAPEV 405
            F   YVATRWYRAPE+
Sbjct:  200 FLTEYVATRWYRAPEI 215

[Close]

BLAST of EY679271 vs. ExPASy Swiss-Prot
Match: ERKA_DROME (Mitogen-activated protein kinase ERK-A OS=Drosophila melanogaster GN=rl PE=1 SV=2)

HSP 1 Score: 119.398 bits (298), Expect = 3.806e-29
Identity = 58/121 (47.93%), Postives = 85/121 (70.25%), Query Frame = 3

Query:    6 RILREVKLLRLLRHPDIVEIKRIMLPPSKREFKDIYVVFELMESDLHQVIKANDDLTREHHQFFLYQMLRALKYMHTANVYHRDLKPKNILANANCKLKVCDFGLARVAFSDTPMTVFWTE 368
            R LRE+ +L   +H +I++I+ I+   S  + +D+Y+V  LME+DL++++K    L+ +H  +FLYQ+LR LKY+H+ANV HRDLKP N+L N  C LK+CDFGLAR+A  +   T F TE
Sbjct:  130 RTLREITILTRFKHENIIDIRDILRVDSIDQMRDVYIVQCLMETDLYKLLKTQR-LSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNKTCDLKICDFGLARIADPEHDHTGFLTE 249

HSP 2 Score: 30.8018 bits (68), Expect = 3.806e-29
Identity = 12/16 (75.00%), Postives = 13/16 (81.25%), Query Frame = 1

Query:  358 FGRNYVATRWYRAPEV 405
            F   YVATRWYRAPE+
Sbjct:  246 FLTEYVATRWYRAPEI 261

[Close]

BLAST of EY679271 vs. ExPASy Swiss-Prot
Match: NLK_CAEEL (Serine/threonine kinase NLK OS=Caenorhabditis elegans GN=lit-1 PE=1 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 7.853e-28
Identity = 54/107 (50.47%), Postives = 81/107 (75.70%), Query Frame = 3

Query:    6 RILREVKLLRLLRHPDIVEIKRIMLPPSKREFKDIYVVFELMESDLHQVIKANDDLTREHHQFFLYQMLRALKYMHTANVYHRDLKPKNILANANCKLKVCDFGLAR 326
            R+ RE+K+L   RH +++ +  I+ P +   F+++YV+ ELM+SDLH++I +   LT +H + F+YQ+LR LKY+HTAN+ HRD+KP N+L N+NC LK+CDFGLAR
Sbjct:  283 RVFREIKMLSSFRHDNVLSLLDILQPANPSFFQELYVLTELMQSDLHKIIVSPQALTPDHVKVFVYQILRGLKYLHTANILHRDIKPGNLLVNSNCILKICDFGLAR 389

[Close]

BLAST of EY679271 vs. ExPASy Swiss-Prot
Match: NLK_MOUSE (Serine/threonine protein kinase NLK OS=Mus musculus GN=Nlk PE=1 SV=2)

HSP 1 Score: 123.635 bits (309), Expect = 1.524e-27
Identity = 55/108 (50.93%), Postives = 80/108 (74.07%), Query Frame = 3

Query:    6 RILREVKLLRLLRHPDIVEIKRIMLPPSKREFKDIYVVFELMESDLHQVIKANDDLTREHHQFFLYQMLRALKYMHTANVYHRDLKPKNILANANCKLKVCDFGLARV 329
            R+ RE+K+L   +H +++    I+ PP    F++IYVV ELM+SDLH++I +   L+ +H + FLYQ+LR LKY+H+A + HRD+KP N+L N+NC LK+CDFGLARV
Sbjct:  181 RVFRELKMLCFFKHDNVLSALDILQPPHIDYFEEIYVVTELMQSDLHKIIVSPQPLSSDHVKVFLYQILRGLKYLHSAGILHRDIKPGNLLVNSNCVLKICDFGLARV 288

HSP 2 Score: 21.1718 bits (43), Expect = 1.524e-27
Identity = 7/11 (63.64%), Postives = 10/11 (90.91%), Query Frame = 1

Query:  373 VATRWYRAPEV 405
            V T++YRAPE+
Sbjct:  301 VVTQYYRAPEI 311

[Close]

BLAST of EY679271 vs. ExPASy Swiss-Prot
Match: NLK_HUMAN (Serine/threonine protein kinase NLK OS=Homo sapiens GN=NLK PE=1 SV=2)

HSP 1 Score: 123.635 bits (309), Expect = 1.524e-27
Identity = 55/108 (50.93%), Postives = 80/108 (74.07%), Query Frame = 3

Query:    6 RILREVKLLRLLRHPDIVEIKRIMLPPSKREFKDIYVVFELMESDLHQVIKANDDLTREHHQFFLYQMLRALKYMHTANVYHRDLKPKNILANANCKLKVCDFGLARV 329
            R+ RE+K+L   +H +++    I+ PP    F++IYVV ELM+SDLH++I +   L+ +H + FLYQ+LR LKY+H+A + HRD+KP N+L N+NC LK+CDFGLARV
Sbjct:  181 RVFRELKMLCFFKHDNVLSALDILQPPHIDYFEEIYVVTELMQSDLHKIIVSPQPLSSDHVKVFLYQILRGLKYLHSAGILHRDIKPGNLLVNSNCVLKICDFGLARV 288

HSP 2 Score: 21.1718 bits (43), Expect = 1.524e-27
Identity = 7/11 (63.64%), Postives = 10/11 (90.91%), Query Frame = 1

Query:  373 VATRWYRAPEV 405
            V T++YRAPE+
Sbjct:  301 VVTQYYRAPEI 311

The following BLAST results are available for this feature:

BLAST of EY679271 vs. ExPASy Swiss-Prot

BLAST of EY679271 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 493


Match Name	E-value	Identity	Description
MK03_RAT	7.275e-31	47.93	Mitogen-activated protein kinase 3 OS=Rattus norve...	[more]
MK03_MOUSE	7.275e-31	47.93	Mitogen-activated protein kinase 3 OS=Mus musculus...	[more]
SUR1_CAEEL	2.716e-30	50.41	Mitogen-activated protein kinase mpk-1 OS=Caenorha...	[more]
MPK12_ARATH	4.619e-30	49.56	Mitogen-activated protein kinase 12 OS=Arabidopsis...	[more]
FUS3_YEAST	4.623e-30	51.38	Mitogen-activated protein kinase FUS3 OS=Saccharom...	[more]
MK03_HUMAN	6.012e-30	47.11	Mitogen-activated protein kinase 3 OS=Homo sapiens...	[more]
ERKA_DROME	3.806e-29	47.93	Mitogen-activated protein kinase ERK-A OS=Drosophi...	[more]
NLK_CAEEL	7.853e-28	50.47	Serine/threonine kinase NLK OS=Caenorhabditis eleg...	[more]
NLK_MOUSE	1.524e-27	50.93	Serine/threonine protein kinase NLK OS=Mus musculu...	[more]
NLK_HUMAN	1.524e-27	50.93	Serine/threonine protein kinase NLK OS=Homo sapien...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C1-401-058-D02-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY679271 ID=EY679271; Name=EY679271; organism=Citrus sinensis; type=EST; length=999bp
CAATTCGGATCCTGCGTGAAGTCAAGTTGCTTAGGCTTTTACGACATCCT
GATATTGTTGAAATTAAGCGTATCATGTTGCCACCCTCAAAGAGAGAGTT
TAAAGACATATATGTTGTATTTGAGCTAATGGAGTCTGACCTGCACCAAG
TCATCAAAGCTAATGATGATTTGACCCGTGAACACCATCAGTTTTTCCTT
TACCAAATGCTACGTGCATTGAAATATATGCATACAGCAAATGTGTACCA
CCGGGATCTTAAACCGAAAAATATATTGGCAAATGCCAACTGCAAACTCA
AAGTCTGTGATTTTGGATTAGCAAGAGTTGCATTTAGTGATACTCCAATG
ACTGTCTTTTGGACGGAATTATGTGGCTACCAGATGGTACAGGGCTCCAG
AGGTGTGGTGGGATCTTTCTTTTTCAAAGGGAGGACTATGAACTCTCCTG
GGAGTTGAAAATATCTGTCTTAACGTTATACACCTGCAGATGAAAATTTG
AAGTAGTTGACTGTATTTTTTGTCTGAGGAATTGAACGAGGAAACCATTT
GTTCCTTTGTAAAATGTTGATGTTTGTAAATTTTTATTGTATTTCTTAAT
TTATTACGAACCACCTTATATAAGAAAAGAATTGGTGTTGGATTATAAAT
GCAAAAACATGAAAAATTATTTGGAATACATTTTATGCAAAATGCATGAC
TGTGAAAAAGTATTGCAAAGGGGGCACCAAGGAAAAAAGGTCATCTTTAA
CACTTTGGAGAAGTAGTAAAACGTAGAGAGTAGAGAGTCCCCGTCGGGTG
TAGTAATCATAGATCTAGGCTATGAAGTACTTATGTCCGATTATCATAAT
CTGTTTCGACAGTTAGCTCATGGATAACGAAATTAAGAATCAAAATTGAA
ATAATCATAATTATTAAGCATTATATTTCAATTAAATGATTAACGAGAGA
TATTGGTTTCATTCAAATAGAAGATGATTGGCGAATTGTGATATGTGCT