EY679184

Overview
NameEY679184
Unique NameEY679184
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length907
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY679184 vs. ExPASy Swiss-Prot
Match: MK03_RAT (Mitogen-activated protein kinase 3 OS=Rattus norvegicus GN=Mapk3 PE=1 SV=5)

HSP 1 Score: 62.3882 bits (150), Expect = 3.330e-19
Identity = 25/52 (48.08%), Postives = 38/52 (73.08%), Query Frame = 1 
Query:   10 RAPELLLNSSDYTAVIYVWSVGCTFMELMNRRPLFPGKDHVHQMRLLIELLG 165
            RAPE++LNS  YT  I +WSVGC   E+++ RP+FPGK ++ Q+  ++ +LG
Sbjct:  212 RAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILG 263

HSP 2 Score: 54.299 bits (129), Expect = 3.330e-19
Identity = 27/53 (50.94%), Postives = 36/53 (67.92%), Query Frame = 3 
Query:  210 ANRYIQQLPLHPRDSVAQVFPHVLPLAIDLVYRMLTFDPMTRITVDEPLAHPY 368
            A  Y+Q LP   + + A++FP     A+DL+ RMLTF+P  RITV+E LAHPY
Sbjct:  278 ARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPY 330
[Close]
BLAST of EY679184 vs. ExPASy Swiss-Prot
Match: MK03_MOUSE (Mitogen-activated protein kinase 3 OS=Mus musculus GN=Mapk3 PE=1 SV=5)

HSP 1 Score: 62.3882 bits (150), Expect = 3.330e-19
Identity = 25/52 (48.08%), Postives = 38/52 (73.08%), Query Frame = 1 
Query:   10 RAPELLLNSSDYTAVIYVWSVGCTFMELMNRRPLFPGKDHVHQMRLLIELLG 165
            RAPE++LNS  YT  I +WSVGC   E+++ RP+FPGK ++ Q+  ++ +LG
Sbjct:  212 RAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILG 263

HSP 2 Score: 54.299 bits (129), Expect = 3.330e-19
Identity = 27/53 (50.94%), Postives = 36/53 (67.92%), Query Frame = 3 
Query:  210 ANRYIQQLPLHPRDSVAQVFPHVLPLAIDLVYRMLTFDPMTRITVDEPLAHPY 368
            A  Y+Q LP   + + A++FP     A+DL+ RMLTF+P  RITV+E LAHPY
Sbjct:  278 ARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPY 330
[Close]
BLAST of EY679184 vs. ExPASy Swiss-Prot
Match: MK03_HUMAN (Mitogen-activated protein kinase 3 OS=Homo sapiens GN=MAPK3 PE=1 SV=4)

HSP 1 Score: 62.3882 bits (150), Expect = 3.330e-19
Identity = 25/52 (48.08%), Postives = 38/52 (73.08%), Query Frame = 1 
Query:   10 RAPELLLNSSDYTAVIYVWSVGCTFMELMNRRPLFPGKDHVHQMRLLIELLG 165
            RAPE++LNS  YT  I +WSVGC   E+++ RP+FPGK ++ Q+  ++ +LG
Sbjct:  211 RAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILG 262

HSP 2 Score: 54.299 bits (129), Expect = 3.330e-19
Identity = 27/53 (50.94%), Postives = 36/53 (67.92%), Query Frame = 3 
Query:  210 ANRYIQQLPLHPRDSVAQVFPHVLPLAIDLVYRMLTFDPMTRITVDEPLAHPY 368
            A  Y+Q LP   + + A++FP     A+DL+ RMLTF+P  RITV+E LAHPY
Sbjct:  277 ARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPY 329
[Close]
BLAST of EY679184 vs. ExPASy Swiss-Prot
Match: MPK7_ARATH (Mitogen-activated protein kinase 7 OS=Arabidopsis thaliana GN=MPK7 PE=2 SV=2)

HSP 1 Score: 61.2326 bits (147), Expect = 4.334e-19
Identity = 26/52 (50.00%), Postives = 39/52 (75.00%), Query Frame = 1 
Query:   10 RAPELLLNSSDYTAVIYVWSVGCTFMELMNRRPLFPGKDHVHQMRLLIELLG 165
            RAPELLL   +Y   I VWSVGC F E++ R+P+FPG + ++Q++L+I ++G
Sbjct:  200 RAPELLLCCDNYGTSIDVWSVGCIFAEILGRKPIFPGTECLNQLKLIINVVG 251

HSP 2 Score: 55.0694 bits (131), Expect = 4.334e-19
Identity = 26/55 (47.27%), Postives = 35/55 (63.64%), Query Frame = 3 
Query:  210 ANRYIQQLPLHPRDSVAQVFPHVLPLAIDLVYRMLTFDPMTRITVDEPLAHPYPA 374
            A R+I+ LP      ++ ++P   PLAIDL+ RML FDP  RI+V + L HPY A
Sbjct:  266 ARRFIKSLPYSRGTHLSNLYPQANPLAIDLLQRMLVFDPTKRISVTDALLHPYMA 320
[Close]
BLAST of EY679184 vs. ExPASy Swiss-Prot
Match: HOG1_CHAGB (Mitogen-activated protein kinase HOG1 OS=Chaetomium globosum GN=HOG1 PE=3 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 4.335e-19
Identity = 26/54 (48.15%), Postives = 37/54 (68.52%), Query Frame = 1 
Query:    4 FTRAPELLLNSSDYTAVIYVWSVGCTFMELMNRRPLFPGKDHVHQMRLLIELLG 165
            + RAPE++L    Y   + +WS GC F E++  +PLFPGKDHV+Q  ++ ELLG
Sbjct:  178 YYRAPEIMLTWQKYDVEVDIWSAGCIFAEMLEGKPLFPGKDHVNQFSIITELLG 231

HSP 2 Score: 51.6026 bits (122), Expect = 4.335e-19
Identity = 25/53 (47.17%), Postives = 32/53 (60.38%), Query Frame = 3 
Query:  216 RYIQQLPLHPRDSVAQVFPHVLPLAIDLVYRMLTFDPMTRITVDEPLAHPYPA 374
            R+++ LP+  R  +   F +  P AIDL+ RML FDP  RIT  E LAH Y A
Sbjct:  248 RFVKSLPMRERQPLKNKFKNADPTAIDLLERMLVFDPKKRITATEALAHEYLA 300
[Close]
BLAST of EY679184 vs. ExPASy Swiss-Prot
Match: HOG1_BOTFB (Mitogen-activated protein kinase hog1 OS=Botryotinia fuckeliana (strain B05.10) GN=hog1 PE=1 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 5.640e-19
Identity = 27/54 (50.00%), Postives = 37/54 (68.52%), Query Frame = 1 
Query:    4 FTRAPELLLNSSDYTAVIYVWSVGCTFMELMNRRPLFPGKDHVHQMRLLIELLG 165
            + RAPE++L    Y   + VWS GC F E++  +PLFPGKDHV+Q  ++ ELLG
Sbjct:  178 YYRAPEIMLTWQKYDVEVDVWSAGCIFAEMLEGKPLFPGKDHVNQFSIITELLG 231

HSP 2 Score: 50.8322 bits (120), Expect = 5.640e-19
Identity = 25/51 (49.02%), Postives = 30/51 (58.82%), Query Frame = 3 
Query:  216 RYIQQLPLHPRDSVAQVFPHVLPLAIDLVYRMLTFDPMTRITVDEPLAHPY 368
            R++Q LP   R  +A  F    PLAIDL+ +ML FDP  RI   E LAH Y
Sbjct:  248 RFVQSLPKRERQPLASKFTQADPLAIDLLEKMLVFDPRARIKAAEGLAHEY 298
[Close]
BLAST of EY679184 vs. ExPASy Swiss-Prot
Match: HOG1_COCHE (Mitogen-activated protein kinase HOG1 OS=Cochliobolus heterostrophus GN=HOG1 PE=1 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 7.334e-19
Identity = 26/54 (48.15%), Postives = 37/54 (68.52%), Query Frame = 1 
Query:    4 FTRAPELLLNSSDYTAVIYVWSVGCTFMELMNRRPLFPGKDHVHQMRLLIELLG 165
            + RAPE++L    Y   + +WS GC F E++  +PLFPGKDHV+Q  ++ ELLG
Sbjct:  178 YYRAPEIMLTWQKYDVEVDIWSAGCIFAEMLEGKPLFPGKDHVNQFSIITELLG 231

HSP 2 Score: 50.8322 bits (120), Expect = 7.334e-19
Identity = 23/53 (43.40%), Postives = 32/53 (60.38%), Query Frame = 3 
Query:  216 RYIQQLPLHPRDSVAQVFPHVLPLAIDLVYRMLTFDPMTRITVDEPLAHPYPA 374
            R++Q LP   R  +A  F +  P A+DL+  ML FDP  R+  ++ LAHPY A
Sbjct:  248 RFVQSLPKRERQPLANKFKNAEPDAVDLLENMLVFDPRKRVRAEQALAHPYLA 300
[Close]
BLAST of EY679184 vs. ExPASy Swiss-Prot
Match: HOG1_SETTU (Mitogen-activated protein kinase HOG1 OS=Setosphaeria turcica GN=HOG1 PE=2 SV=2)

HSP 1 Score: 64.6994 bits (156), Expect = 7.349e-19
Identity = 26/54 (48.15%), Postives = 37/54 (68.52%), Query Frame = 1 
Query:    4 FTRAPELLLNSSDYTAVIYVWSVGCTFMELMNRRPLFPGKDHVHQMRLLIELLG 165
            + RAPE++L    Y   + +WS GC F E++  +PLFPGKDHV+Q  ++ ELLG
Sbjct:  151 YYRAPEIMLTWQKYDVEVDIWSAGCIFAEMLEGKPLFPGKDHVNQFSIITELLG 204

HSP 2 Score: 50.8322 bits (120), Expect = 7.349e-19
Identity = 23/53 (43.40%), Postives = 32/53 (60.38%), Query Frame = 3 
Query:  216 RYIQQLPLHPRDSVAQVFPHVLPLAIDLVYRMLTFDPMTRITVDEPLAHPYPA 374
            R++Q LP   R  +A  F +  P A+DL+  ML FDP  R+  ++ LAHPY A
Sbjct:  221 RFVQPLPKRERQPLANKFKNAEPEAVDLLENMLVFDPRKRVRAEQALAHPYLA 273
[Close]
BLAST of EY679184 vs. ExPASy Swiss-Prot
Match: HOG1_CRYNV (Mitogen-activated protein kinase HOG1 OS=Cryptococcus neoformans var. grubii GN=HOG1 PE=1 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 9.532e-19
Identity = 26/54 (48.15%), Postives = 37/54 (68.52%), Query Frame = 1 
Query:    4 FTRAPELLLNSSDYTAVIYVWSVGCTFMELMNRRPLFPGKDHVHQMRLLIELLG 165
            + RAPE++L    Y   + +WS GC F E++  +PLFPGKDHV+Q  ++ ELLG
Sbjct:  178 YYRAPEIMLTWQKYDVAVDIWSTGCIFAEMLEGKPLFPGKDHVNQFSIITELLG 231

HSP 2 Score: 49.6766 bits (117), Expect = 9.532e-19
Identity = 23/53 (43.40%), Postives = 34/53 (64.15%), Query Frame = 3 
Query:  216 RYIQQLPLHPRDSVAQVFPHVLPLAIDLVYRMLTFDPMTRITVDEPLAHPYPA 374
            R++Q LP   +   +  FP+  P+++DL+ +ML FDP TRI+  E LAH Y A
Sbjct:  248 RFVQSLPKREKVPFSTKFPNADPVSLDLLEKMLVFDPRTRISAAEGLAHEYLA 300
[Close]
BLAST of EY679184 vs. ExPASy Swiss-Prot
Match: HOG1_CRYNE (Mitogen-activated protein kinase HOG1 OS=Cryptococcus neoformans GN=HOG1 PE=1 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 9.532e-19
Identity = 26/54 (48.15%), Postives = 37/54 (68.52%), Query Frame = 1 
Query:    4 FTRAPELLLNSSDYTAVIYVWSVGCTFMELMNRRPLFPGKDHVHQMRLLIELLG 165
            + RAPE++L    Y   + +WS GC F E++  +PLFPGKDHV+Q  ++ ELLG
Sbjct:  178 YYRAPEIMLTWQKYDVAVDIWSTGCIFAEMLEGKPLFPGKDHVNQFSIITELLG 231

HSP 2 Score: 49.6766 bits (117), Expect = 9.532e-19
Identity = 23/53 (43.40%), Postives = 34/53 (64.15%), Query Frame = 3 
Query:  216 RYIQQLPLHPRDSVAQVFPHVLPLAIDLVYRMLTFDPMTRITVDEPLAHPYPA 374
            R++Q LP   +   +  FP+  P+++DL+ +ML FDP TRI+  E LAH Y A
Sbjct:  248 RFVQSLPKREKVPFSTKFPNADPVSLDLLEKMLVFDPRTRISAAEGLAHEYLA 300
The following BLAST results are available for this feature:
BLAST of EY679184 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 194
Match NameE-valueIdentityDescription
MK03_RAT3.330e-1948.08Mitogen-activated protein kinase 3 OS=Rattus norve... [more]
MK03_MOUSE3.330e-1948.08Mitogen-activated protein kinase 3 OS=Mus musculus... [more]
MK03_HUMAN3.330e-1948.08Mitogen-activated protein kinase 3 OS=Homo sapiens... [more]
MPK7_ARATH4.334e-1950.00Mitogen-activated protein kinase 7 OS=Arabidopsis ... [more]
HOG1_CHAGB4.335e-1948.15Mitogen-activated protein kinase HOG1 OS=Chaetomiu... [more]
HOG1_BOTFB5.640e-1950.00Mitogen-activated protein kinase hog1 OS=Botryotin... [more]
HOG1_COCHE7.334e-1948.15Mitogen-activated protein kinase HOG1 OS=Cochliobo... [more]
HOG1_SETTU7.349e-1948.15Mitogen-activated protein kinase HOG1 OS=Setosphae... [more]
HOG1_CRYNV9.532e-1948.15Mitogen-activated protein kinase HOG1 OS=Cryptococ... [more]
HOG1_CRYNE9.532e-1948.15Mitogen-activated protein kinase HOG1 OS=Cryptococ... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-056-C06-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY679184 ID=EY679184; Name=EY679184; organism=Citrus sinensis; type=EST; length=907bp
CTTTTTACACGAGCCCCTGAGTTGTTATTGAACTCTTCTGACTACACTGC
TGTCATATATGTGTGGTCTGTTGGCTGTACATTTATGGAGCTTATGAACA
GAAGGCCTCTGTTCCCAGGAAAAGATCACGTGCATCAAATGCGCTTATTG
ATAGAGCTTCTTGGAATCACCTACAGATGCTGATCTGGGGTTTGTCCGTA
CTCGATGATGCAAACAGATATATACAACAACTTCCCCTGCATCCTCGTGA
TTCGGTAGCCCAAGTTTTTCCGCATGTTCTTCCACTGGCCATAGATCTTG
TATACAGAATGCTTACCTTTGATCCCATGACTAGAATTACTGTTGACGAA
CCACTAGCCCATCCTTATCCTGCAAGAATTACATGACGAAAGCCGATGAC
ACGGTCTGCCCAGAATCATTCTCTTTTGATTTTCAGCATCAATGCCTGAT
GAAAAAACAAAAAAAACGACTTTGTTTATTAAGGGGACTTTACACTGAAT
CCGGTTTTGGCTTAAAATAGAAATCTCAATGGGGGCTTCGTTTTACAAAT
CACTATTTGCTGGATTACAATATCTCAGGAGAGCTCGGAAGTAATAGATG
ACTTGAAACCCATATAAACAAAGAAGGACGGCAAAAATACTCGCCACAGT
AGTTTTATTTGAATGGACCAAAAACGGGTTACCCTTAATTACTTTTCTCC
TTTTTTTTTTTTCTTGAACACGTTTCTCTCCTTGATTGCTTTCTTCCGCG
TCTAGATAATAAATCTAAAATAGTTATTTGTCTATTATACAAATTCTAAC
AAGTGAATAGAATAGATTAATATGGGGGCCCACTTCTGTTTCTTAAATAG
CGGCAAAGGGTTCTTTTCCTTATTATATATATTTTTATCTCATCCCCCTC
CCATTTA
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