EY744547

Overview
NameEY744547
Unique NameEY744547
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length933
Libraries
Library NameType
Sweet orange flower, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY744547 vs. ExPASy Swiss-Prot
Match: NOS1_HUMAN (Nitric oxide synthase, brain OS=Homo sapiens GN=NOS1 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 2.273e-18
Identity = 53/136 (38.97%), Postives = 77/136 (56.62%), Query Frame = 2 
Query:   53 GRVHKGLCSTWMKNSLPMEKSNDCSWAPIFVRQS-NFKLPADAKVPIIMIGPGTGLPPFRGFLQER-FALQEAGA*LGPSLLFFGCRNRKMDYIYEDELNNFVQSGALSQLIVAFSRE-GPTKEYVQHKMMEKSSD 451
            G +H G+CS+W+      E        P FVR + +F LP + +VP I++GPGTG+ PFR F Q+R F +Q  G    P +L FGCR  K+D+IY +E       G   +L  A+SRE    K+YVQ  + E+ ++
Sbjct: 1209 GPIHHGVCSSWLNRIQADEL------VPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEQLAE 1338
[Close]
BLAST of EY744547 vs. ExPASy Swiss-Prot
Match: CYSJ_CITK8 (Sulfite reductase [NADPH] flavoprotein alpha-component OS=Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) GN=cysJ PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 2.734e-18
Identity = 51/136 (37.50%), Postives = 83/136 (61.03%), Query Frame = 2 
Query:   53 GRVHKGLCSTWMKNSLPMEKSNDCSWAPIFVRQS-NFKLPADAKVPIIMIGPGTGLPPFRGFLQERFALQEAGA*LGPSLLFFGCRNRKMDYIYEDELNNFVQSGALSQLIVAFSREGPTKEYVQHKMMEKSSDIW 457
            GR   G  S+++ + +  E         +F+  + NF+LPA+ + P+IMIGPGTG+ PFR F+Q+R A  EA    G + LFFG  +   D++Y+ E   +V+ G LS++ +A+SR+   K YVQ K+ E+ +++W
Sbjct:  416 GRARAGGASSFLADRVEEEGE-----VRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQR-AADEAP---GKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLSRIDLAWSRDQKEKIYVQDKLREQGAELW 542

HSP 2 Score: 21.9422 bits (45), Expect = 2.734e-18
Identity = 6/11 (54.55%), Postives = 11/11 (100.00%), Query Frame = 3 
Query:  465 LSEGAYLYVCG 497
            +++GA++YVCG
Sbjct:  545 INDGAHIYVCG 555
[Close]
BLAST of EY744547 vs. ExPASy Swiss-Prot
Match: NOS3_CANFA (Nitric oxide synthase, endothelial OS=Canis familiaris GN=NOS3 PE=2 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.969e-18
Identity = 52/127 (40.94%), Postives = 75/127 (59.06%), Query Frame = 2 
Query:   53 GRVHKGLCSTWMKNSLPMEKSNDCSWAPIFVRQS-NFKLPADAKVPIIMIGPGTGLPPFRGFLQERF-ALQEAGA*LGPSLLFFGCRNRKMDYIYEDELNNFVQSGALSQLIVAFSREGPT-KEYVQ 424
            G +H G+CSTW+       K+ D    P F+R + +F+LP D  +P I++GPGTG+ PFRGF Q R   +   G    P  L FGCR  ++D++Y DE+ +  Q G   +++ AFSRE  + K YVQ
Sbjct:  971 GPLHYGVCSTWLSQL----KAGDP--VPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQGRLHDIYSKGLQPAPMTLVFGCRCSQLDHLYRDEVQDAQQRGVFGRVLTAFSREPDSPKTYVQ 1091
[Close]
BLAST of EY744547 vs. ExPASy Swiss-Prot
Match: NOS2_MOUSE (Nitric oxide synthase, inducible OS=Mus musculus GN=Nos2 PE=1 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.969e-18
Identity = 53/127 (41.73%), Postives = 72/127 (56.69%), Query Frame = 2 
Query:   53 GRVHKGLCSTWMKNSLPMEKSNDCSWAPIFVRQ-SNFKLPADAKVPIIMIGPGTGLPPFRGFLQERF-ALQEAGA*LGPSLLFFGCRNRKMDYIYEDELNNFVQSGALSQLIVAFSR-EGPTKEYVQ 424
            G +H G+CSTW++N  P +        P FVR  S F+LP D   P I+IGPGTG+ PFR F Q+R    Q  G   G   L FGCR+ + D++Y++E+   V+   L Q+   +SR  G  K YVQ
Sbjct:  931 GPLHHGVCSTWIRNLKPQDP------VPCFVRSVSGFQLPEDPSQPCILIGPGTGIAPFRSFWQQRLHDSQHKGLKGGRMSLVFGCRHPEEDHLYQEEMQEMVRKRVLFQVHTGYSRLPGKPKVYVQ 1051
[Close]
BLAST of EY744547 vs. ExPASy Swiss-Prot
Match: CYSJ_MANSM (Sulfite reductase [NADPH] flavoprotein alpha-component OS=Mannheimia succiniciproducens (strain MBEL55E) GN=cysJ PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 3.555e-18
Identity = 53/133 (39.85%), Postives = 77/133 (57.89%), Query Frame = 2 
Query:   62 HKGLCSTWMKNSLPMEKSNDCSWAPIFVRQS-NFKLPADAKVPIIMIGPGTGLPPFRGFLQERFALQEAGA*LGPSLLFFGCRNRKMDYIYEDELNNFVQSGALSQLIVAFSREGPTKEYVQHKMMEKSSDIW 457
            H+G   T + +    ++  +     IFV  + NF+LP D   PIIMIG GTG+ PFR FLQ+R A +EA    G + L FG ++   D++Y+ E   FV+ G L +   A+SR+   K YVQ K+ EKS+ +W
Sbjct:  410 HEGRARTGVASGFLADRVEEDGEVKIFVEPNDNFRLPQDKSKPIIMIGSGTGIAPFRAFLQQRQA-EEAE---GKNWLIFGNQHFATDFLYQAEWQQFVKDGYLHKYDFAWSRDQAEKIYVQDKIREKSTALW 538

HSP 2 Score: 24.2534 bits (51), Expect = 3.555e-18
Identity = 9/15 (60.00%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  465 LSEGAYLYVCGGCPK 509
            L EGA++YVCG   K
Sbjct:  541 LQEGAHVYVCGDASK 555
[Close]
BLAST of EY744547 vs. ExPASy Swiss-Prot
Match: CYSJ_YERE8 (Sulfite reductase [NADPH] flavoprotein alpha-component OS=Yersinia enterocolitica serotype O:8 / biotype 1B (strain 8081) GN=cysJ PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 4.621e-18
Identity = 45/109 (41.28%), Postives = 72/109 (66.06%), Query Frame = 2 
Query:  137 IFVRQS-NFKLPADAKVPIIMIGPGTGLPPFRGFLQERFALQEAGA*LGPSLLFFGCRNRKMDYIYEDELNNFVQSGALSQLIVAFSREGPTKEYVQHKMMEKSSDIWN 460
            IF+  + NF+LPA+ + P+IMIGPGTG+ PFR F+Q+R    EA    G + L FG  +   D++Y+ E   +V+ G L+++ +A+SR+   K YVQ K+ E+ +++WN
Sbjct:  439 IFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQR----EADGATGKNWLLFGNPHFTEDFLYQVEWQRYVKDGLLTRIDLAWSRDQADKIYVQDKLREQGAELWN 543

HSP 2 Score: 21.557 bits (44), Expect = 4.621e-18
Identity = 6/11 (54.55%), Postives = 10/11 (90.91%), Query Frame = 3 
Query:  465 LSEGAYLYVCG 497
            + +GA++YVCG
Sbjct:  545 IQQGAHIYVCG 555
[Close]
BLAST of EY744547 vs. ExPASy Swiss-Prot
Match: CYSJ_SHISS (Sulfite reductase [NADPH] flavoprotein alpha-component OS=Shigella sonnei (strain Ss046) GN=cysJ PE=3 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 4.621e-18
Identity = 50/136 (36.76%), Postives = 83/136 (61.03%), Query Frame = 2 
Query:   53 GRVHKGLCSTWMKNSLPMEKSNDCSWAPIFVRQS-NFKLPADAKVPIIMIGPGTGLPPFRGFLQERFALQEAGA*LGPSLLFFGCRNRKMDYIYEDELNNFVQSGALSQLIVAFSREGPTKEYVQHKMMEKSSDIW 457
            GR   G  S+++ + +  E         +F+  + NF+LPA+ + P+IMIGPGTG+ PFR F+Q+R A  EA    G + LFFG  +   D++Y+ E   +V+ G L+++ +A+SR+   K YVQ K+ E+ +++W
Sbjct:  414 GRARAGGASSFLADRVEEEGE-----VRVFIEHNDNFRLPANPQTPVIMIGPGTGIAPFRAFMQQR-AADEAP---GKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLREQGAELW 540

HSP 2 Score: 21.9422 bits (45), Expect = 4.621e-18
Identity = 6/11 (54.55%), Postives = 11/11 (100.00%), Query Frame = 3 
Query:  465 LSEGAYLYVCG 497
            +++GA++YVCG
Sbjct:  543 INDGAHIYVCG 553
[Close]
BLAST of EY744547 vs. ExPASy Swiss-Prot
Match: CYSJ_SHIFL (Sulfite reductase [NADPH] flavoprotein alpha-component OS=Shigella flexneri GN=cysJ PE=3 SV=3)

HSP 1 Score: 90.5077 bits (223), Expect = 4.621e-18
Identity = 50/136 (36.76%), Postives = 83/136 (61.03%), Query Frame = 2 
Query:   53 GRVHKGLCSTWMKNSLPMEKSNDCSWAPIFVRQS-NFKLPADAKVPIIMIGPGTGLPPFRGFLQERFALQEAGA*LGPSLLFFGCRNRKMDYIYEDELNNFVQSGALSQLIVAFSREGPTKEYVQHKMMEKSSDIW 457
            GR   G  S+++ + +  E         +F+  + NF+LPA+ + P+IMIGPGTG+ PFR F+Q+R A  EA    G + LFFG  +   D++Y+ E   +V+ G L+++ +A+SR+   K YVQ K+ E+ +++W
Sbjct:  414 GRARAGGASSFLADRVEEEGE-----VRVFIEHNDNFRLPANLETPVIMIGPGTGIAPFRAFMQQR-AADEAP---GKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLREQGAELW 540

HSP 2 Score: 21.9422 bits (45), Expect = 4.621e-18
Identity = 6/11 (54.55%), Postives = 11/11 (100.00%), Query Frame = 3 
Query:  465 LSEGAYLYVCG 497
            +++GA++YVCG
Sbjct:  543 INDGAHIYVCG 553
[Close]
BLAST of EY744547 vs. ExPASy Swiss-Prot
Match: CYSJ_SHIF8 (Sulfite reductase [NADPH] flavoprotein alpha-component OS=Shigella flexneri serotype 5b (strain 8401) GN=cysJ PE=3 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 4.621e-18
Identity = 50/136 (36.76%), Postives = 83/136 (61.03%), Query Frame = 2 
Query:   53 GRVHKGLCSTWMKNSLPMEKSNDCSWAPIFVRQS-NFKLPADAKVPIIMIGPGTGLPPFRGFLQERFALQEAGA*LGPSLLFFGCRNRKMDYIYEDELNNFVQSGALSQLIVAFSREGPTKEYVQHKMMEKSSDIW 457
            GR   G  S+++ + +  E         +F+  + NF+LPA+ + P+IMIGPGTG+ PFR F+Q+R A  EA    G + LFFG  +   D++Y+ E   +V+ G L+++ +A+SR+   K YVQ K+ E+ +++W
Sbjct:  414 GRARAGGASSFLADRVEEEGE-----VRVFIEHNDNFRLPANLETPVIMIGPGTGIAPFRAFMQQR-AADEAP---GKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLREQGAELW 540

HSP 2 Score: 21.9422 bits (45), Expect = 4.621e-18
Identity = 6/11 (54.55%), Postives = 11/11 (100.00%), Query Frame = 3 
Query:  465 LSEGAYLYVCG 497
            +++GA++YVCG
Sbjct:  543 INDGAHIYVCG 553
[Close]
BLAST of EY744547 vs. ExPASy Swiss-Prot
Match: CYSJ_SHIDS (Sulfite reductase [NADPH] flavoprotein alpha-component OS=Shigella dysenteriae serotype 1 (strain Sd197) GN=cysJ PE=3 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 4.621e-18
Identity = 50/136 (36.76%), Postives = 83/136 (61.03%), Query Frame = 2 
Query:   53 GRVHKGLCSTWMKNSLPMEKSNDCSWAPIFVRQS-NFKLPADAKVPIIMIGPGTGLPPFRGFLQERFALQEAGA*LGPSLLFFGCRNRKMDYIYEDELNNFVQSGALSQLIVAFSREGPTKEYVQHKMMEKSSDIW 457
            GR   G  S+++ + +  E         +F+  + NF+LPA+ + P+IMIGPGTG+ PFR F+Q+R A  EA    G + LFFG  +   D++Y+ E   +V+ G L+++ +A+SR+   K YVQ K+ E+ +++W
Sbjct:  414 GRTRAGGASSFLADRVEEEGE-----VRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQR-AADEAP---GKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLREQGAELW 540

HSP 2 Score: 21.9422 bits (45), Expect = 4.621e-18
Identity = 6/11 (54.55%), Postives = 11/11 (100.00%), Query Frame = 3 
Query:  465 LSEGAYLYVCG 497
            +++GA++YVCG
Sbjct:  543 INDGAHIYVCG 553
The following BLAST results are available for this feature:
BLAST of EY744547 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 117
Match NameE-valueIdentityDescription
NOS1_HUMAN2.273e-1838.97Nitric oxide synthase, brain OS=Homo sapiens GN=NO... [more]
CYSJ_CITK82.734e-1837.50Sulfite reductase [NADPH] flavoprotein alpha-compo... [more]
NOS3_CANFA2.969e-1840.94Nitric oxide synthase, endothelial OS=Canis famili... [more]
NOS2_MOUSE2.969e-1841.73Nitric oxide synthase, inducible OS=Mus musculus G... [more]
CYSJ_MANSM3.555e-1839.85Sulfite reductase [NADPH] flavoprotein alpha-compo... [more]
CYSJ_YERE84.621e-1841.28Sulfite reductase [NADPH] flavoprotein alpha-compo... [more]
CYSJ_SHISS4.621e-1836.76Sulfite reductase [NADPH] flavoprotein alpha-compo... [more]
CYSJ_SHIFL4.621e-1836.76Sulfite reductase [NADPH] flavoprotein alpha-compo... [more]
CYSJ_SHIF84.621e-1836.76Sulfite reductase [NADPH] flavoprotein alpha-compo... [more]
CYSJ_SHIDS4.621e-1836.76Sulfite reductase [NADPH] flavoprotein alpha-compo... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C5-003-009-G09-CT.F Sweet orange flower, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY744547 ID=EY744547; Name=EY744547; organism=Citrus sinensis; type=EST; length=933bp
CCACGCGTCCGATTCATGTTACCTGTGCTCTGGATTATGAGAAACACCAA
CAGGACGTGTTCACAAAGGATTGTGCTCCACTTGGATGAAGAATTCTTTG
CCAATGGAGAAAAGCAATGACTGCAGCTGGGCACCCATTTTTGTTAGGCA
ATCAAACTTCAAACTCCCTGCAGATGCGAAAGTGCCTATCATTATGATTG
GCCCTGGCACTGGATTACCTCCTTTCAGGGGTTTCCTACAGGAAAGGTTT
GCTCTGCAAGAAGCTGGAGCATAACTGGGACCCTCTCTATTATTTTTCGG
ATGCAGGAACCGTAAGATGGATTACATTTATGAGGATGAGCTCAACAACT
TTGTTCAAAGTGGTGCACTTTCACAGTTGATTGTTGCCTTCTCACGTGAG
GGACCCACCAAGGAGTATGTGCAGCATAAAATGATGGAGAAGTCTTCAGA
TATCTGGAACATGCCTTTCTGAGGGAGCTTACTTGTATGTGTGTGGGGGA
TGCCCAAAAGCATGGCTACAGAATGTCCACCCGAACTCCGGCCCATATTT
GCTCCCAACCTGGCACCCCTAAACAGCTCACAAACGGACAAAGTGGGGAA
CAAAATTCACCAATTGCTTGCACAGGACAACACCCGATGCCCGGCCGACC
CTCCCCCCCCAACCGGGACCCCATCATCCAATGAAACGGAAGAGCAGGGA
GGGGCGGGACCAACGACCATAAGCAATTAAGTCCCGGCGGACATTACCAA
TCAGCAACGACCCAAAACGAAAAGGCGCACCTCGCAGCGATTCGACCCCC
CCACGGCCACCCCGACCAAGCAGACAACCAGCCCCCTCGCCTCCATACCA
CACCATTTCCCAACCCACCCCCCGATACACCACACTCGATACCTCAGCAC
CGACACCCCACACATCCCACGCCACACCCCGCG
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