EY744506 | Citrus Genome Database

Overview


Name	EY744506
Unique Name	EY744506
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	975

Libraries


Library Name	Type
Sweet orange flower, greenhouse plant	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY744506 vs. ExPASy Swiss-Prot
Match: IF4A_NEUCR (ATP-dependent RNA helicase eIF4A OS=Neurospora crassa GN=tif-1 PE=3 SV=2)

HSP 1 Score: 135.191 bits (339), Expect = 3.542e-33
Identity = 65/99 (65.66%), Postives = 81/99 (81.82%), Query Frame = 1

Query:  151 EAITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSSTEVQALILSPTRELATQTEKVILAIGDFI 447
            E + SFD M +K +LLRGIY YGFE+PSAIQQRA+MP+IKG DVIAQAQSGTGKT+  +++V Q +D S    QALIL+PTRELA Q +KV++AIGDF+
Sbjct:   21 ETVDSFDEMNLKPELLRGIYAYGFERPSAIQQRAIMPVIKGHDVIAQAQSGTGKTATFSISVLQKIDPSLKACQALILAPTRELAQQIQKVVVAIGDFM 119

HSP 2 Score: 26.9498 bits (58), Expect = 3.542e-33
Identity = 10/22 (45.45%), Postives = 16/22 (72.73%), Query Frame = 2

Query:  446 LNIRAPACVGGKSVGEDIRKLR 511
            +NI   AC+GG SV +D++ L+
Sbjct:  119 MNIECHACIGGTSVRDDMKALQ 140

HSP 3 Score: 21.9422 bits (45), Expect = 3.542e-33
Identity = 10/21 (47.62%), Postives = 13/21 (61.90%), Query Frame = 3

Query:  510 EHGLDVVSGTPAAL*DMITRK 572
            + G  VV GTP  + DMI R+
Sbjct:  140 QDGPQVVVGTPGRVHDMIQRR 160

[Close]

BLAST of EY744506 vs. ExPASy Swiss-Prot
Match: IF4A_CHAGB (ATP-dependent RNA helicase eIF4A OS=Chaetomium globosum GN=TIF1 PE=3 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 5.977e-33
Identity = 64/99 (64.65%), Postives = 81/99 (81.82%), Query Frame = 1

Query:  151 EAITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSSTEVQALILSPTRELATQTEKVILAIGDFI 447
            E + SFD M +K +LLRGIY YGFE+PSAIQQRA+MP+IKG DVIAQAQSGTGKT+  +++V Q +D +    QALIL+PTRELA Q +KV++AIGDF+
Sbjct:   21 ETVDSFDDMNLKSELLRGIYAYGFERPSAIQQRAIMPVIKGHDVIAQAQSGTGKTATFSISVLQKIDPTVKSCQALILAPTRELAQQIQKVVIAIGDFM 119

HSP 2 Score: 26.9498 bits (58), Expect = 5.977e-33
Identity = 10/22 (45.45%), Postives = 16/22 (72.73%), Query Frame = 2

Query:  446 LNIRAPACVGGKSVGEDIRKLR 511
            +NI   AC+GG SV +D++ L+
Sbjct:  119 MNIECHACIGGTSVRDDMKALQ 140

HSP 3 Score: 21.557 bits (44), Expect = 5.977e-33
Identity = 9/21 (42.86%), Postives = 13/21 (61.90%), Query Frame = 3

Query:  510 EHGLDVVSGTPAAL*DMITRK 572
            + G  V+ GTP  + DMI R+
Sbjct:  140 QDGPQVIVGTPGRVHDMIQRR 160

[Close]

BLAST of EY744506 vs. ExPASy Swiss-Prot
Match: FAL1_PICST (ATP-dependent RNA helicase FAL1 OS=Pichia stipitis GN=FAL1 PE=3 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 7.763e-33
Identity = 61/113 (53.98%), Postives = 85/113 (75.22%), Query Frame = 1

Query:  133 ETTEGVEAITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSSTEVQALILSPTRELATQTEKVILAIGDFIK-HTSTCV 468
            ++T+ V+   +F++M +K DLL+GIY YGFE PSAIQ RA+M II GRD IAQAQSGTGKT+  ++ + Q +DT++ + QALILSPTRELA Q + V+  +GD++  HT  C+
Sbjct:   17 KSTKNVKVHATFESMNLKPDLLKGIYGYGFEAPSAIQSRAIMQIINGRDTIAQAQSGTGKTATFSIGMLQAIDTNAKDCQALILSPTRELAVQIQNVVKHLGDYMNIHTHACI 129

HSP 2 Score: 31.9574 bits (71), Expect = 7.763e-33
Identity = 12/22 (54.55%), Postives = 17/22 (77.27%), Query Frame = 2

Query:  446 LNIRAPACVGGKSVGEDIRKLR 511
            +NI   AC+GG  VG+DI+KL+
Sbjct:  121 MNIHTHACIGGTHVGDDIKKLK 142

HSP 3 Score: 23.483 bits (49), Expect = 7.763e-33
Identity = 9/21 (42.86%), Postives = 14/21 (66.67%), Query Frame = 3

Query:  510 EHGLDVVSGTPAAL*DMITRK 572
            + G  +VSGTP  + DM+ R+
Sbjct:  142 KQGQQIVSGTPGRVVDMVKRQ 162

[Close]

BLAST of EY744506 vs. ExPASy Swiss-Prot
Match: FAL1_DEBHA (ATP-dependent RNA helicase FAL1 OS=Debaryomyces hansenii GN=FAL1 PE=3 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 7.763e-33
Identity = 59/113 (52.21%), Postives = 84/113 (74.34%), Query Frame = 1

Query:  133 ETTEGVEAITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSSTEVQALILSPTRELATQTEKVILAIGDFIK-HTSTCV 468
            ++T+ ++   +F++M +K DLL+GIY YGFE PSAIQ RA+M II G+D IAQAQSGTGKT+  ++ + + +DT S + QALILSPTRELA Q + V+  +GD++  HT  C+
Sbjct:   17 KSTKNIKVHATFESMNLKTDLLKGIYGYGFEAPSAIQSRAIMQIISGKDTIAQAQSGTGKTATFSIGMLEVIDTKSKDCQALILSPTRELAQQIQSVVKHLGDYMNVHTHACI 129

HSP 2 Score: 32.3426 bits (72), Expect = 7.763e-33
Identity = 12/22 (54.55%), Postives = 17/22 (77.27%), Query Frame = 2

Query:  446 LNIRAPACVGGKSVGEDIRKLR 511
            +N+   AC+GG  VGEDI+KL+
Sbjct:  121 MNVHTHACIGGTHVGEDIKKLQ 142

HSP 3 Score: 25.409 bits (54), Expect = 7.763e-33
Identity = 11/24 (45.83%), Postives = 15/24 (62.50%), Query Frame = 3

Query:  510 EHGLDVVSGTPAAL*DMITRKPFA 581
            + G  +VSGTP  + DMI R+  A
Sbjct:  142 QQGQQIVSGTPGRVVDMIKRRNLA 165

[Close]

BLAST of EY744506 vs. ExPASy Swiss-Prot
Match: IF4A_DROME (Eukaryotic initiation factor 4A OS=Drosophila melanogaster GN=eIF-4a PE=2 SV=3)

HSP 1 Score: 137.117 bits (344), Expect = 1.067e-32
Identity = 63/107 (58.88%), Postives = 88/107 (82.24%), Query Frame = 1

Query:  151 EAITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSSTEVQALILSPTRELATQTEKVILAIGDFIK-HTSTCV 468
            E   +FD M ++++LLRGIY YGFEKPSAIQQRA++P ++GRDVIAQAQSGTGKT+  ++ + Q +DTS  E QALIL+PTRELATQ ++V++A+G+++K H+  C+
Sbjct:   27 EVYDNFDDMNLREELLRGIYGYGFEKPSAIQQRAIIPCVRGRDVIAQAQSGTGKTATFSIAILQQIDTSIRECQALILAPTRELATQIQRVVMALGEYMKVHSHACI 133

HSP 2 Score: 25.0238 bits (53), Expect = 1.067e-32
Identity = 12/21 (57.14%), Postives = 13/21 (61.90%), Query Frame = 3

Query:  510 EHGLDVVSGTPAAL*DMITRK 572
            E G  VV GTP  + DMI RK
Sbjct:  146 ESGCHVVVGTPGRVYDMINRK 166

[Close]

BLAST of EY744506 vs. ExPASy Swiss-Prot
Match: FAL1_PICGU (ATP-dependent RNA helicase FAL1 OS=Pichia guilliermondii GN=FAL1 PE=3 SV=2)

HSP 1 Score: 122.479 bits (306), Expect = 2.872e-32
Identity = 58/105 (55.24%), Postives = 81/105 (77.14%), Query Frame = 1

Query:  133 ETTEGVEAITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSSTEVQALILSPTRELATQTEKVILAIGDFI 447
            ++T+ V+   +F++M +K +LL+GIY YGFE PSAIQ RA+M II+GRD IAQAQSGTGKT+  ++ +  ++DT S + QAL+LSPTRELA Q E VI  +GD++
Sbjct:   15 KSTKKVKIYPTFESMNLKPELLKGIYNYGFEAPSAIQSRAIMQIIRGRDTIAQAQSGTGKTATFSIGILSSIDTKSKDCQALVLSPTRELAQQIENVIEHLGDYM 119

HSP 2 Score: 34.6538 bits (78), Expect = 2.872e-32
Identity = 12/22 (54.55%), Postives = 19/22 (86.36%), Query Frame = 2

Query:  446 LNIRAPACVGGKSVGEDIRKLR 511
            +N+R+ AC+GG  VGED++KL+
Sbjct:  119 MNVRSHACIGGTQVGEDVKKLQ 140

HSP 3 Score: 23.8682 bits (50), Expect = 2.872e-32
Identity = 9/21 (42.86%), Postives = 14/21 (66.67%), Query Frame = 3

Query:  510 EHGLDVVSGTPAAL*DMITRK 572
            + G  ++SGTP  + DMI R+
Sbjct:  140 QQGQHIISGTPGRVLDMIKRR 160

[Close]

BLAST of EY744506 vs. ExPASy Swiss-Prot
Match: IF4A_AJECN (ATP-dependent RNA helicase eIF4A OS=Ajellomyces capsulata (strain NAm1 / WU24) GN=TIF1 PE=3 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 4.850e-32
Identity = 64/99 (64.65%), Postives = 81/99 (81.82%), Query Frame = 1

Query:  151 EAITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSSTEVQALILSPTRELATQTEKVILAIGDFI 447
            E   SFDAM ++ +LLRG+Y YGFE+PSAIQQRA+MP+IKG DVIAQAQSGTGKT+  +++  Q VDT+    QALIL+PTRELA Q +KV++AIGDF+
Sbjct:   22 EITDSFDAMNLRAELLRGVYAYGFERPSAIQQRAIMPVIKGSDVIAQAQSGTGKTATFSISALQKVDTNLKACQALILAPTRELAQQIQKVVVAIGDFM 120

HSP 2 Score: 25.409 bits (54), Expect = 4.850e-32
Identity = 10/22 (45.45%), Postives = 16/22 (72.73%), Query Frame = 2

Query:  446 LNIRAPACVGGKSVGEDIRKLR 511
            ++I   AC+GG SV ED++ L+
Sbjct:  120 MSIECHACIGGISVREDMKALQ 141

HSP 3 Score: 21.9422 bits (45), Expect = 4.850e-32
Identity = 10/21 (47.62%), Postives = 13/21 (61.90%), Query Frame = 3

Query:  510 EHGLDVVSGTPAAL*DMITRK 572
            + G  VV GTP  + DMI R+
Sbjct:  141 QDGPQVVVGTPGRVHDMIQRR 161

[Close]

BLAST of EY744506 vs. ExPASy Swiss-Prot
Match: IF4A_COCIM (ATP-dependent RNA helicase eIF4A OS=Coccidioides immitis GN=TIF1 PE=3 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 6.294e-32
Identity = 64/99 (64.65%), Postives = 81/99 (81.82%), Query Frame = 1

Query:  151 EAITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSSTEVQALILSPTRELATQTEKVILAIGDFI 447
            E   SFDAM ++ +LLRG+Y YGFE+PSAIQQRA+MP+IKG DVIAQAQSGTGKT+  +++  Q VDT+    QALIL+PTRELA Q +KV++AIGDF+
Sbjct:   22 EITDSFDAMNLRAELLRGVYAYGFERPSAIQQRAIMPVIKGSDVIAQAQSGTGKTATFSISALQKVDTNLKACQALILAPTRELAQQIQKVVVAIGDFM 120

HSP 2 Score: 25.409 bits (54), Expect = 6.294e-32
Identity = 9/21 (42.86%), Postives = 15/21 (71.43%), Query Frame = 2

Query:  446 LNIRAPACVGGKSVGEDIRKL 508
            +++   AC+GG +V EDI+ L
Sbjct:  120 MSVECHACIGGTNVREDIKAL 140

HSP 3 Score: 21.557 bits (44), Expect = 6.294e-32
Identity = 10/19 (52.63%), Postives = 12/19 (63.16%), Query Frame = 3

Query:  516 GLDVVSGTPAAL*DMITRK 572
            G  VV GTP  + DMI R+
Sbjct:  143 GPQVVVGTPGRVHDMIQRR 161

[Close]

BLAST of EY744506 vs. ExPASy Swiss-Prot
Match: IF4A_SCHPO (ATP-dependent RNA helicase eIF4A OS=Schizosaccharomyces pombe GN=tif1 PE=1 SV=2)

HSP 1 Score: 129.413 bits (324), Expect = 6.297e-32
Identity = 62/99 (62.63%), Postives = 81/99 (81.82%), Query Frame = 1

Query:  151 EAITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSSTEVQALILSPTRELATQTEKVILAIGDFI 447
            E I +FD M +K +LLRGIY YGFE+PSAIQQRA+MPI+  RDV+AQAQSGTGKT+  +++V Q +DTS  + QALIL+PTRELA Q +KV++A+GD +
Sbjct:   16 EVIDTFDDMNLKPELLRGIYAYGFERPSAIQQRAIMPILGERDVLAQAQSGTGKTATFSISVLQKIDTSLKQCQALILAPTRELAQQIQKVVVALGDLM 114

HSP 2 Score: 26.1794 bits (56), Expect = 6.297e-32
Identity = 10/24 (41.67%), Postives = 16/24 (66.67%), Query Frame = 2

Query:  443 LLNIRAPACVGGKSVGEDIRKLRA 514
            L+N+   AC+GG  V +D+  L+A
Sbjct:  113 LMNVECHACIGGTLVRDDMAALQA 136

HSP 3 Score: 24.2534 bits (51), Expect = 6.297e-32
Identity = 13/25 (52.00%), Postives = 17/25 (68.00%), Query Frame = 3

Query:  516 GLDVVSGTPAAL*DMITRKPFAVPT 590
            G+ VV GTP  + DMI R+  A+PT
Sbjct:  137 GVHVVVGTPGRVHDMIQRR--ALPT 159

[Close]

BLAST of EY744506 vs. ExPASy Swiss-Prot
Match: IF4A_USTMA (ATP-dependent RNA helicase eIF4A OS=Ustilago maydis GN=TIF1 PE=3 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 8.166e-32
Identity = 60/98 (61.22%), Postives = 81/98 (82.65%), Query Frame = 1

Query:  157 ITSFDAMGIKDDLLRGIYQYGFEKPSAIQQRAVMPIIKGRDVIAQAQSGTGKTSMIALTVCQTVDTSSTEVQALILSPTRELATQTEKVILAIGDFIK 450
            I +FD M +K++LLRG+Y YGFE+PSAIQ RA++P+IKG DVIAQAQSGTGKT+  ++ + Q +D S   VQALIL+PTRELA Q +KV++A+GD++K
Sbjct:   37 IDNFDNMELKEELLRGVYAYGFERPSAIQARAIVPVIKGHDVIAQAQSGTGKTATFSIAILQRIDPSIKAVQALILAPTRELAQQIQKVVIALGDYMK 134

HSP 2 Score: 25.0238 bits (53), Expect = 8.166e-32
Identity = 12/26 (46.15%), Postives = 14/26 (53.85%), Query Frame = 3

Query:  516 GLDVVSGTPAAL*DMITRKPFAVPTL 593
            G  VV GTP  + DMI R+ F    L
Sbjct:  156 GAQVVVGTPGRVYDMINRRAFKTDQL 181

HSP 3 Score: 24.6386 bits (52), Expect = 8.166e-32
Identity = 10/21 (47.62%), Postives = 14/21 (66.67%), Query Frame = 2

Query:  446 LNIRAPACVGGKSVGEDIRKL 508
            + I   AC+GG +V ED+ KL
Sbjct:  133 MKIDCHACIGGTNVREDMAKL 153

The following BLAST results are available for this feature:

BLAST of EY744506 vs. ExPASy Swiss-Prot

BLAST of EY744506 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 287


Match Name	E-value	Identity	Description
IF4A_NEUCR	3.542e-33	65.66	ATP-dependent RNA helicase eIF4A OS=Neurospora cra...	[more]
IF4A_CHAGB	5.977e-33	64.65	ATP-dependent RNA helicase eIF4A OS=Chaetomium glo...	[more]
FAL1_PICST	7.763e-33	53.98	ATP-dependent RNA helicase FAL1 OS=Pichia stipitis...	[more]
FAL1_DEBHA	7.763e-33	52.21	ATP-dependent RNA helicase FAL1 OS=Debaryomyces ha...	[more]
IF4A_DROME	1.067e-32	58.88	Eukaryotic initiation factor 4A OS=Drosophila mela...	[more]
FAL1_PICGU	2.872e-32	55.24	ATP-dependent RNA helicase FAL1 OS=Pichia guillier...	[more]
IF4A_AJECN	4.850e-32	64.65	ATP-dependent RNA helicase eIF4A OS=Ajellomyces ca...	[more]
IF4A_COCIM	6.294e-32	64.65	ATP-dependent RNA helicase eIF4A OS=Coccidioides i...	[more]
IF4A_SCHPO	6.297e-32	62.63	ATP-dependent RNA helicase eIF4A OS=Schizosaccharo...	[more]
IF4A_USTMA	8.166e-32	61.22	ATP-dependent RNA helicase eIF4A OS=Ustilago maydi...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C5-003-009-D04-CT.F Sweet orange flower, greenhouse plant Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY744506 ID=EY744506; Name=EY744506; organism=Citrus sinensis; type=EST; length=975bp
CCACGCGTCCGCTCTCATTGATTCTTTGATTTCGAAGAGTAATTGTTGCG
AATTTCGTGCGAGATGGCGGCGGCGACGGCTATGCGGAGGAGAGGAGGGG
GCGGCGGAATGGATGACGACAAGATGGTGTTCGAAACAACGGAAGGAGTG
GAGGCAATCACGAGCTTCGACGCAATGGGAATAAAAGACGATCTGTTGCG
TGGAATTTATCAGTACGGATTCGAGAAGCCATCGGCCATACAACAGAGGG
CCGTTATGCCAATAATAAAGGGCCGTGATGTAATTGCACAGGCCCAGTCT
GGTACTGGCAAGACCTCTATGATTGCCCTAACTGTTTGCCAGACGGTCGA
TACTTCTAGCACAGAGGTGCAGGCGTTGATTTTGTCACCTACGAGGGAGT
TGGCGACGCAGACAGAGAAAGTGATTTTGGCAATTGGTGATTTTATTAAA
CATACGAGCACCTGCGTGTGTTGGAGGCAAAAGTGTCGGGGAAGATATTA
GGAAGTTAAGAGCATGGACTTGATGTGGTGTCTGGAACTCCTGCTGCACT
CTGAGATATGATAACCAGGAAACCTTTTGCCGTACCTACACTTATTAATT
TATTTGGAGCCTCCCTACAACCCCCATCATGTGCTGACGCAACAGGTCCC
TGGAGAAACCTCTTCATAGGTGCCTGCCAAATTTCACCCAAATCTTTCCG
GGCAGTATCACCACAACCACGGCCCTTCTTCTTCATCCCATTGGCACACG
ACCTACCACCTCCAAAAAACCACCATCGCTCAAAAAAATCTCGGGCCAAA
ACCCGCTACGTGTACCTTTTTTAATGCGCCCCACCACCCTACTCTTCTAG
TCCTGGTCTGGGCGAGCTAGAATCAGCACGCCCGGTCCATCTATTAACTC
CCCCGATACTACATTCTATTCTATTTCCGCTACACACCACAGCAACGCAA
ACAACCAGCAACTCTACTGACTCCG